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Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization

Investigation article published in BMC Microbiology

March 16th, 2010

Lactococcus garvieae is a bacterial pathogen that affects different animal species in addition to humans. Despite the widespread distribution and emerging clinical significance of L. garvieae in both veterinary and human medicine, there is almost a complete lack of knowledge about the genetic content of this microorganism. In the present study, the genomic content of L. garvieae CECT 4531 was analysed using bioinformatics tools and microarray-based comparative genomic hybridization (CGH) experiments. Lactococcus lactis subsp. lactis IL1403 and Streptococcus pneumoniae TIGR4 were used as reference microorganisms.

The combination and integration of in silico analyses and in vitro (CGH) experiments performed in comparison with the reference microorganisms allowed establishment of an inter-species hybridization framework with a detection threshold based on a sequence similarity of [greater than or equal to] 70%. With this threshold value, 267 genes were identified as having an analogue in L. garvieae, most of which (n = 258) have been documented for the first time in this pathogen. Most of the genes are related to ribosomal, sugar metabolism or energy conversion systems. Some of the identified genes, such as als and mycA, could be involved in the pathogenesis of L. garvieae infections.

In this study, we identified 267 genes that were potentially present in L. garvieae CECT 4531. Some of the identified genes could be involved in the pathogenesis of L. garvieae infections. These results provide the first insight into the genome content of L. garvieae




Aguado-Urda M., Lopez-Campos GH., Fernandez-Garayzabal JF., Martin-Sanchez F., Gibello A., Dominguez L. and Blanco MM.




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Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization

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Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization



Participants:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto de Salud Carlos IIICentro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP). Instituto de Salud Carlos III (ISCIII).







BMC Microbiology
FACTOR YEAR Q
2.960 2010

NLMID: 100966981

PMID: 20233401

ISSN: 1471-2180



TITLE: Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization


JOURNAL: BMC Microbiol.


NUMERACIÓN: 10:79


AÑO: 2010


PUBLISHER: BioMed Central


AUTHORS: Aguado-Urda M., Lopez-Campos GH., Fernandez-Garayzabal JF., Martin-Sanchez F., Gibello A., Dominguez L. and Blanco MM.


VISAVET PARTICIPANTS


3rd
José Francisco Fernández-Garayzabal Fernández
6th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1186/1471-2180-10-79


CITE THIS PUBLICATION:

Aguado-Urda M., Lopez-Campos GH., Fernandez-Garayzabal JF., Martin-Sanchez F., Gibello A., Dominguez L. and Blanco MM. Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization. BMC Microbiology. 10:79. 2010. (A). ISSN: 1471-2180. DOI: 10.1186/1471-2180-10-79


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