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PCR amplification and high resolution melt curve analysis as a rapid diagnostic method to genotype members of the Mycobacterium avium-intracellulare complex

Clinical Microbiology and Infection publish this investigation article

November 1st, 2010

Some of the members of the Mycobacterium avium-intracellulare (MAI) complex are recognised as human pathogens in both immunocompromised and immunocompetent patients. The current molecular methods that are available to genotype the MAI complex members can be both expensive and technically demanding. In this report, we describe for the first time the application of a real-time PCR and high resolution melt approach to differentiate between the complex members by targeting a member of the PPE (Pro-Pro-Glu) gene family, MACPPE24. In this aim, reference strains of the Mycobacterium avium subspecies and Mycobacterium intracellulare (M. intracellulare) were used to optimise the technique. Then, this real time PCR-HRM approach was used to distinguish 10 Mycobacterium avium subspecies hominissuis field isolates from the M. intracellulare reference strain




Castellanos E., Aranaz A. and De Buck J.




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PCR amplification and high resolution melt curve analysis as a rapid diagnostic method to genotype members of the Mycobacterium avium-intracellulare complex

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PCR amplification and high resolution melt curve analysis as a rapid diagnostic method to genotype members of the Mycobacterium avium-intracellulare complex



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Department of Production Animal Health. Faculty of Veterinary Medicine. University of Calgary.







Clinical Microbiology and Infection
FACTOR YEAR Q
4.784 2010

NLMID: 9516420

PMID: 20167007

ISSN: 1198-743X



TITLE: PCR amplification and high resolution melt curve analysis as a rapid diagnostic method to genotype members of the Mycobacterium avium-intracellulare complex


JOURNAL: Clin. Microbiol. Infect.


NUMERACIÓN: 16:1658-1662


AÑO: 2010


PUBLISHER: Blackwell Scientific Publications


AUTHORS: Castellanos E., Aranaz A. and De Buck J.


DOI: https://doi.org/10.1111/j.1469-0691.2010.03198.x


CITE THIS PUBLICATION:

Castellanos E., Aranaz A. and De Buck J. PCR amplification and high resolution melt curve analysis as a rapid diagnostic method to genotype members of the Mycobacterium avium-intracellulare complex. Clinical Microbiology and Infection. 16:1658-1662. 2010. (A). ISSN: 1198-743X. DOI: 10.1111/j.1469-0691.2010.03198.x


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