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Molecular characterization of Mycobacterium avium subspecies paratuberculosis Types II and III isolates by a combination of MIRU-VNTR loci

Investigation article published in Veterinary Microbiology

July 29th, 2010

Mycobacterial interspersed repetitive units and variable number tandem repeats typing (MIRU-VNTR) is a useful technique that has been recently applied to characterize members of the Mycobacterium avium complex (MAC). The aim of this study was to examine the genetic variability among a collection of Spanish M. avium subspecies paratuberculosis (M. a. paratuberculosis) isolates with a combination of MIRU-VNTR loci. For this purpose we tested six MIRU-VNTR loci (MIRU-2, MIRU-3, VNTR-25, VNTR-32, VNTR-292 and VNTR-259) in 70 M. a. paratuberculosis isolates of Types II and III that were recovered from 22 Spanish localities during a nine-year period (1998-2007). The combination of five loci (MIRU-2, MIRU-3, VNTR-25, VNTR-32 and VNTR-259) enabled the differentiation of 12 allelic profiles, with a resulting Hunter and Gaston discriminatory index (HGDI) of 0.84. Moreover, we obtained MIRU-VNTR patterns that were unique for each of the M. a. paratuberculosis types analyzed (II and III); other patterns were host-related or restricted to geographic areas. Therefore, this MIRU-VNTR approach could be a useful sub-typing molecular tool in order to get a better sense of the epidemiology of Johne`s disease. Copyright © 2010 Elsevier B.V. All rights reserved




Castellanos E., Romero B., Rodriguez-Campos S., de Juan L., Bezos J., Mateos A., Dominguez L. and Aranaz A.




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Molecular characterization of Mycobacterium avium subspecies paratuberculosis Types II and III isolates by a combination of MIRU-VNTR loci

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Molecular characterization of Mycobacterium avium subspecies paratuberculosis Types II and III isolates by a combination of MIRU-VNTR loci



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Veterinary Microbiology
FACTOR YEAR Q
3.256 2010

NLMID: 7705469

PMID: 20116185

ISSN: 0378-1135



TITLE: Molecular characterization of Mycobacterium avium subspecies paratuberculosis Types II and III isolates by a combination of MIRU-VNTR loci


JOURNAL: Vet. Microbiol.


NUMERACIÓN: 144:118-126


AÑO: 2010


PUBLISHER: Elsevier Science Publishers


AUTHORS: Castellanos E., Romero B., Rodriguez-Campos S., de Juan L., Bezos J., Mateos A., Dominguez L. and Aranaz A.


VISAVET PARTICIPANTS


2nd
Beatriz Romero Martínez
4th
Lucía de Juan Ferré
5th
Javier Bezos Garrido
7th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.vetmic.2009.12.028


CITE THIS PUBLICATION:

Castellanos E., Romero B., Rodriguez-Campos S., de Juan L., Bezos J., Mateos A., Dominguez L. and Aranaz A. Molecular characterization of Mycobacterium avium subspecies paratuberculosis Types II and III isolates by a combination of MIRU-VNTR loci. Veterinary Microbiology. 144:118-126. 2010. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2009.12.028


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