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Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types using real-time PCR and high resolution melt analysis of the MAP1506 gene

Investigation published in Journal of Clinical Microbiology

April 1st, 2010

High resolution melt (HRM) can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (Proline-Proline-Glutamic acid) gene family




Castellanos E., Aranaz A. and De Buck J.




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Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types using real-time PCR and high resolution melt analysis of the MAP1506 gene

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Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types using real-time PCR and high resolution melt analysis of the MAP1506 gene



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Department of Production Animal Health. Faculty of Veterinary Medicine. University of Calgary.







Journal of Clinical Microbiology
FACTOR YEAR Q
4.220 2010

NLMID: 7505564

PMID: 20129970

ISSN: 0095-1137



TITLE: Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types using real-time PCR and high resolution melt analysis of the MAP1506 gene


JOURNAL: J. Clin. Microbiol.


NUMERACIÓN: 48(4):1474-7


AÑO: 2010


PUBLISHER: American Society for Microbiology


AUTHORS: Castellanos E., Aranaz A. and De Buck J.


DOI: https://doi.org/10.1128/JCM.02484-09


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