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SCI scientific publications 2003

December 1st, 2003

Comparison of enterococcal populations in animals, humans, and the environment--a European study

International Journal Of Food Microbiology 88(2-3):133-45.

     The objectives of the present study were to generate knowledge of enterococcal populations in the food chain, by studying the population structure (in measures of abundance and diversity) among enterococci in different geographical regions and in different parts of the food chain, as well as the similarities between different enterococcal populations. Altogether, 2868 samples were collected from humans (healthy and hospitalised individuals and clinical isolates), animals (slaughterhouse carcasses and farm animals), and the environment (pig farms, sewage, and surface water) in four European countries-Sweden, Denmark, UK, and Spain. The samples were characterised with regard to presence and numbers of enterococci, and eight (for faecal sample… Leer más

Kuhn I., Iversen A., Burman LG., Olsson-Liljequist B., Franklin A., Finn M., Aarestrup F., Seyfarth AM., Blanch AR., Vilanova X., Taylor H., Caplin J., Moreno MA., Dominguez L., Herrero IA. and Mollby R.

November 1st, 2003

Characterization of genetic differences between Mycobacterium avium subsp. paratuberculosis type I and type II isolates

Journal Of Clinical Microbiology 41(11):5215-23.

     A combination of representational difference analysis and comparative DNA sequencing revealed that four type I (sheep) isolates of Mycobacterium avium subsp. paratuberculosis were differentiated from nine type II (bovine) isolates by the presence of an 11-bp insertion in a novel M. avium subsp. paratuberculosis-specific region of genomic DNA. Further, our studies show that M. avium subsp. paratuberculosis type I isolates contain three type-specific loci that are missing in M. avium subsp. paratuberculosis type II but are present in M. avium subsp. avium. Taken together, the results are consistent with the hypothesis that M. avium subsp. paratuberculosis type I strains are an evolutionary intermediate between M. avium subsp. avium and M. avi… Leer más

Dohmann K., Strommenger B., Stevenson K., de Juan L., Stratmann J., Kapur V., Bull TJ. and Gerlach GF.

November 1st, 2003

Elevation of Mycobacterium tuberculosis subsp. caprae Aranaz et al. 1999 to species rank as Mycobacterium caprae comb. nov., sp. nov.

International Journal Of Systematic And Evolutionary Microbiology 53(Pt 6):1785-9.

     Mycobacterium tuberculosis complex isolates recovered from goats were originally classified as Mycobacterium tuberculosis subsp. caprae; however, this subspecies was recently reclassified as Mycobacterium bovis subsp. caprae. Besides biochemical (sensitivity to pyrazinamide) and epidemiological features, strains of this unusual member of the M. tuberculosis complex show a special combination of pncA, oxyR, katG and gyrA gene polymorphisms. Sequence analysis of the gyrB gene in these strains reveals special nucleotide substitutions not found in other members of the M. tuberculosis complex that can be used to differentiate caprine mycobacterial strains from M. bovis and other members of the M. tuberculosis complex. M. tuberculosis subsp. capr… Leer más

Aranaz A., Cousins DV., Mateos A. and Dominguez L..

November 1st, 2003

Corynebacterium suicordis sp. nov., from pigs

International Journal Of Systematic And Evolutionary Microbiology 53(Pt 6):2027-31.

     Nineteen strains of Gram-positive, non-motile, non-spore-forming, catalase-positive, rod-shaped bacteria isolated from pigs were characterized by using biochemical, molecular chemical and molecular genetic methods. Two distinct groups of organisms were discerned, based on their colonial morphology, CAMP (Christie-Atkins-Munch-Petersen) reaction and numerical profile by using the API Coryne system. The first group (13 strains) gave a doubtful discrimination between Corynebacterium striatum and Corynebacterium amycolatum, whilst the second group (six strains) were identified tentatively as Corynebacterium urealyticum. Comparative 16S rRNA gene sequencing studies demonstrated that all of the isolates belonged phylogenetically to the genus CORY… Leer más

Vela AI., Mateos A., Collins MD., Briones V., Hutson RA., Dominguez L. and Fernandez-Garayzabal JF..

October 1st, 2003

Weissella confusa infection in primate (Cercopithecus mona)

Emerging Infectious Diseases 9(10):1307-9.

     We describe systemic infection by Weissella confusa in a mona monkey (Cercopithecus mona) on the basis of microbiologic, molecular genetic, and histologic data. The same strain of W. confusa, as determined by pulsed-field gel electrophoresis, was isolated in pure culture from the primate’s brain, liver, spleen, and intestine. Histologic lesions showed inflammatory infiltrates mainly composed of neutrophils, indicating an acute septicemic process.

Weissella microorganisms are gram-positive, catalase-negative coccobacilli, which have been isolated from a wide variety of habitats such as soil, fresh vegetables, fermented foods, or meat and meat products (1,2). The genus Weissella is peculiar since it currently includes 11 valida… Leer más

Vela AI., Porrero MC., Goyache J., Nieto A., Sanchez C., Briones V., Moreno MA., Dominguez L. and Fernandez-Garayzabal JF..

September 12nd, 2003

Susceptibility of Listeria monocytogenes to antimicrobial peptides

FEMS Microbiology Letters 226(1):101-5.

     We assessed the susceptibility of several pathogenic and non-pathogenic Listeria species to antimicrobial peptides of animal and plant origin. Human defensins and thionins were highly inhibitory, whereas protamine, snakin and magainin showed an intermediate effect. A temperature dependence in the activity of potato defensin was observed for Listeria monocytogenes and Listeria ivanovii. PrfA* L. monocytogenes mutants, that overexpress constitutively PrfA-dependent genes, were sensitive to the peptide independently of the temperature whereas isogenic PrfA(-) derivatives were constitutively resistant. These data indicate that the thermoregulated transcription factor PrfA controls the expression of bacterial products that influence the suscepti… Leer más

Lopez-Solanilla E., Gonzalez-Zorn B., Novella S., Vazquez-Boland JA. and Rodriguez-Palenzuela P.

September 1st, 2003

Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples

Journal Of Clinical Microbiology 41(9):4431-4.

     This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A confirmatory test of the specificity of this method based on restriction endonuclease analysis was also developed… Leer más

Aguero M., Fernandez J., Romero LJ., Sanchez-Mascaraque C., Arias M. and Sanchez-Vizcaino JM..

July 1st, 2003

Rapid identification of Rhodococcus equi by a PCR assay targeting the choE gene

Journal Of Clinical Microbiology 41(7):3241-5.

     The actinomycete Rhodococcus equi is an important pathogen of horses and an emerging opportunistic pathogen of humans. Identification of R. equi by classical bacteriological techniques is sometimes difficult, and misclassification of an isolate is not uncommon. We report here on a specific PCR assay for the rapid and reliable identification of R. equi. It is based on the amplification of a fragment of the choE gene encoding cholesterol oxidase. The choE-based PCR was assessed by using a panel of strains comprising 132 isolates from different sources and of different geographical origins, all initially identified biochemically as R. equi, and 30 isolates of representative non-R. equi actinomycete species, including cholesterol oxidase produc… Leer más

Ladron N., Fernandez M., Aguero J., Gonzalez-Zorn B., Vazquez-Boland JA. and Navas J.

July 1st, 2003

Corynebacterium sphenisci sp. nov., isolated from wild penguins

International Journal Of Systematic And Evolutionary Microbiology 53(Pt 4):1009-12.

     Six unidentified gram-positive, rod-shaped organisms recovered from the cloacae of apparently healthy wild penguins were characterized by phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of a cell wall based on meso-diaminopimelic acid and long-chain cellular fatty acids of the straight-chain saturated and monounsaturated types, consistent with the genus Corynebacterium. Corynomycolic acids, which are characteristic of the genus, were also detected, albeit in small amounts. Comparative 16S rRNA gene sequencing studies showed that the unidentified organisms were phylogenetically related to corynebacteria and represent a novel subline associated with a small subcluster of species that includes Co… Leer más

Goyache J., Ballesteros C., Vela AI., Collins MD., Briones V., Hutson RA., Potti J., Garcia-Borboroglu P., Dominguez L. and Fernandez-Garayzabal JF..

July 1st, 2003

Isolation of Corynebacterium falsenii and description of Corynebacterium aquilae sp. nov., from eagles

International Journal Of Systematic And Evolutionary Microbiology 53(Pt 4):1135-8.

     Biochemical, molecular chemical and molecular genetic studies were performed on seven unidentified gram-positive, rod-shaped organisms recovered from eagles. The strains were provisionally identified as Corynebacterium jeikeium with the commercial API Coryne system, but they were able to grow under anaerobic conditions and were non-lipophilic. Comparative 16S rRNA gene sequencing studies demonstrated that the isolates belonged phylogenetically to the genus Corynebacterium. Three strains were identified genotypically as Corynebacterium falsenii; the remaining four strains corresponded to a hitherto unknown lineage within the genus Corynebacterium, associated with a small subcluster of species that included Corynebacterium diphtheriae and its… Leer más

Fernandez-Garayzabal JF., Egido R., Vela AI., Briones V., Collins MD., Mateos A., Hutson RA., Dominguez L. and Goyache J..

June 1st, 2003

Analysis of genetic diversity of Streptococcus suis clinical isolates from pigs in Spain by pulsed-field gel electrophoresis

Journal Of Clinical Microbiology 41(6):2498-502.

     Pulsed-field gel electrophoresis (PFGE) was used to investigate the diversity of Streptococcus suis isolates of various serotypes recovered from swine clinical samples in Spain. Capsular types 9 (64.9%) and 2 (14.8%) were the most frequently isolated serotypes followed by serotype 7 (5.9%) and serotype 8 (4.3%). The PFGE results of this study with 60 different pulsotypes indicate a great genetic diversity among the S. suis isolates, which is consistent with the broad distribution of S. suis in the swine population. Forty-five percent of the pulsotypes corresponded to single isolates, no pulsotype was common to all farms, and at least 3 different pulsotypes were isolated in 56% of herds in which more than 3 clinical isolates were analyzed. T… Leer más

Vela AI., Goyache J., Tarradas C., Luque I., Mateos A., Moreno MA., Borge C., Perea JA., Dominguez L. and Fernandez-Garayzabal JF..

June 1st, 2003

Detection of CMY-2, CTX-M-14, and SHV-12 beta-lactamases in Escherichia coli fecal-sample isolates from healthy chickens

Antimicrobial Agents And Chemotherapy 47(6):2056-8.

     Genes encoding the CMY-2, CTX-M-14, and SHV-12 beta-lactamases were detected in three of five Escherichia coli isolates from fecal samples from healthy chickens which showed resistance or diminished susceptibility to extended-spectrum cephalosporins. A -42 mutation at the promoter region of the ampC gene was detected in the other two isolates… Leer más

Brinas L., Moreno MA., Zarazaga M., Porrero MC., Saenz Y., Garcia M., Dominguez L. and Torres C.

April 1st, 2003

Early detection of PrPres in BSE-infected bovine PrP transgenic mice

Archives Of Virology 148(4):677-91.

     Transgenic mouse lines expressing different levels of the bovine prion protein gene (boPrP(C)) were generated. Upon infection with BSE prions, all transgenic lines tested exhibited characteristics of the bovine disease. Typical CNS spongiform degeneration was observed by histopathology and presence of PrP(res) could be detected both by Western blot and immunohistochemistry (IHC) assays, confirming for this model the absence of an interspecies barrier to BSE infection. Differences in incubation times post-inoculation depend upon the expression level of boPrP(C) and the amount of prions in the inoculum. In the absence of clinical signs, pathognomonic markers of disease could be detected as early as 150 or 196 days post-inoculation by IHC and … Leer más

Castilla J., Gutierrez-Adan A., Brun A., Pintado B., Ramirez MA., Parra B., Doyle D., Rogers M., Salguero FJ., Sanchez C., Sanchez-Vizcaino JM. and Torres JM.

March 1st, 2003

Psychrobacter pulmonis sp. nov., isolated from the lungs of lambs

International Journal Of Systematic And Evolutionary Microbiology 53(Pt 2):415-9.

     Unusual Gram-negative, catalase- and oxidase-positive, coccus-shaped bacteria isolated from the lungs of two lambs were characterized by phenotypic and molecular-genetic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown isolates were genealogically highly related to each other (99.8% sequence similarity) and represent a novel subline within the genus Psychrobacter. The unknown bacterium was phylogenetically closely related to, but distinct from, Psychrobacter phenylpyruvicus, Psychrobacter immobilis, Psychrobacter glacincola and Psychrobacter urativorans. The novel Psychrobacter isolates were readily distinguished from all other Psychrobacter species and other Gram-negative, oxidase-positive bacteria usuall… Leer más

Vela AI., Collins MD., Latre MV., Mateos A., Moreno MA., Hutson RA., Dominguez L. and Fernandez-Garayzabal JF..

February 1st, 2003

VanA-mediated high level glycopeptide resistance in MRSA

The Lancet Infectious Diseases 3(2):67-8.

     We recently encountered what many consider to be the most important
event of the past 20 years in bacterial antibiotic resistance—namely, the isolation in hospitals in Michigan (July 2002) and Pennsylvania (October 2002) of two distinct meticillinresistant Staphylococcus aureus (MRSA)that are also resistant to high concentrations of vancomycin… Leer más

Gonzalez-Zorn B. and Courvalin P.

January 1st, 2003

Detection of foot-and-mouth disease virus from culture and clinical samples by reverse transcription-PCR coupled to restriction enzyme and sequence analysis

Veterinary Research 34(1):105-17.

     A reverse transcription-PCR (RT-PCR) method is presented for the highly sensitive and specific detection of foot-and-mouth disease virus (FMDV). A primer pair flanking a region of the viral polymerase gene (3D) corresponding to the C-terminus of the protein was designed and a single step RT-PCR reaction was developed. The assay allowed the detection of viral RNA from a variety of animal samples and from a wide range of FMDV isolates of different origins and serotypes. The presence of an Ahd I restriction site within the amplicon in 96% of the isolates analyzed allowed an additional confirmation step of the positive reactions by a simple digestion yielding characteristic fragment sizes. The set of primers described here was suitable for dire… Leer más

Saiz M., de la Morena DB., Blanco E., Nunez JL., Fernandez R. and Sanchez-Vizcaino JM..

January 1st, 2003

Corynebacterium spheniscorum sp. nov., isolated from the cloacae of wild penguins

International Journal Of Systematic And Evolutionary Microbiology 53(Pt 1):43-6.

     Twenty unidentified Gram-positive, rod-shaped organisms were recovered from the cloacae of apparently healthy wild penguins (Spheniscus magellanicus) and subjected to a polyphasic taxonomic analysis. On the basis of cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Corynebacterium, although the organisms did not appear to correspond to any recognized species. Lipid studies confirmed this generic placement, and comparative 16S rRNA gene sequencing showed that the unidentified organisms represent a hitherto unknown subline, associated with a small subcluster of species that includes Corynebacterium diphtheriae and its close relatives. On the basis of phenotypic and phylogenetic evidence, it is p… Leer más

Goyache J., Vela AI., Collins MD., Ballesteros C., Briones V., Moreno J., Yorio P., Dominguez L., Hutson RA. and Fernandez-Garayzabal JF..

Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ZTA 250 SUAT 61 SUAT 15
2 ICM 237 ZTA 53 SAP 11
3 MYC 227 MYC 48 MYC 11
4 SUAT 219 ICM 40 ZTA 6
5 NED 75 SAP 31 ICM 4
  VISAVET 1124 315 55

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.907 ZTA 5.359 ZTA 6.183
2 SAP 3.818 SUAT 4.227 SAP 6.06
3 SUAT 2.987 SAP 4.095 NED 4.3
4 MYC 2.8 NED 3.858 SUAT 4.287
5 DICM 2.776 ICM 3.798 MYC 3.118
VISAVET 3.284 4.885 6.794

ISI Scientific Publications
Total Last 60 mo.
1 Transbound Emerg Dis 93 Front Vet Sci 35
2 Vet Microbiol 88 Transbound Emerg Dis 32
3 Prev Vet Med 77 Animals 15
4 Front Vet Sci 61 Res Vet Sci 14
5 PLoS ONE 56 Sci Rep 14
  All journals 354   All journals 39