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SCI scientific publications 1998

December 1st, 1998

Ribotyping and plasmid profiling of Yersinia ruckeri

Journal Of Applied Microbiology 85(6):949-55.

     A total of 183 strains of Yersinia ruckeri, isolated from a wide variety of sources, were studied with respect to their plasmid profile and ribotype patterns. Eight plasmid profiles and 11 ribotypes were demonstrated, with one profile being predominant by both typing methods. The results suggest a clonal structure for this species, with a predominant clone being responsible for most of the outbreaks worldwide. The results of a long-time survey in several fish farms in Spain and Denmark seem to support this idea… Leer más

Garcia JA., Dominguez L., Larsen JL. and Pedersen K.

December 1st, 1998

Functional similarities between the Listeria monocytogenes virulence regulator PrfA and cyclic AMP receptor protein: the PrfA* (Gly145Ser) mutation increases binding affinity for target DNA

Journal Of Bacteriology 180(24):6655-60.

     Most Listeria monocytogenes virulence genes are positively regulated by the PrfA protein, a transcription factor sharing sequence similarities with cyclic AMP (cAMP) receptor protein (CRP). Its coding gene, prfA, is regulated by PrfA itself via an autoregulatory loop mediated by the upstream PrfA-dependent plcA promoter. We have recently characterized prfA* mutants from L. monocytogenes which, as a result of a single amino acid substitution in PrfA, Gly145Ser, constitutively overexpress prfA and the genes of the PrfA virulence regulon. Here, we show that about 10 times more PrfA protein is produced in a prfA* strain than in the wild type. Thus, the phenotype of prfA* mutants is presumably due to the synthesis of a PrfA protein with higher p… Leer más

Vega Y., Dickneite C., Ripio MT., Bockmann R., Gonzalez-Zorn B., Novella S., Dominguez-Bernal G., Goebel W. and Vazquez-Boland JA.

October 1st, 1998

Streptococcus parasanguinis: new pathogen associated with asymptomatic mastitis in sheep

Emerging Infectious Diseases 4(4):645-7.

     We describe two unusual cases in sheep of subclinical mastitis caused by Streptococcus parasanguinis. This bacterium has been associated with the development of experimental endocarditis; its presence at relatively high concentrations in apparently healthy sheep milk may pose a health risk in persons with predisposing heart lesions… Leer más

Fernandez-Garayzabal JF., Fernandez E., Las Heras A., Pascual C., Collins MD. and Dominguez L..

September 1st, 1998

Haemorrhagic Septicaemia by Aeromonas salmonicida subsp. salmonicida in a Black-tip Reef Shark (Carcharhinus melanopterus)

Journal Of Veterinary Medicine. B, Infectious Diseases And Veterinary Public Health 45(7):443-5.

     One black-tip reef shark (Carcharhinus melanopterus) was found dead without previous signs of disease. Major lesions consisted in cutaneous erythema, mainly at the base of the fins, focal to diffuse inflammatory lesions in gills and intestinal wall, and discrete haemorrhages in the same organs, liver and kidneys. Microcolonies of Gram-negative rods were observed in the lamina propia of gills, underneath the intestinal mucosa and randomly distributed in the renal and hepatic parenchymas. Also, emboli containing Gram-negative rods were observed in capillaries of these organs. Aeromonas salmonicida subsp. salmonicida was isolated in pure culture from gills, liver and intestine. Specific immunostaining confirmed the relationship between the iso… Leer más

Briones V., Fernandez A., Blanco M., Ramiro F., Vicente ML., Garcia J., Mendez JL. and Goyache J..

April 15th, 1998

Restriction fragment length polymorphism and spacer oligonucleotide typing: a comparative analysis of fingerprinting strategies for Mycobacterium bovis

Veterinary Microbiology 61(4):311-24.

     The combination of conventional investigation and DNA fingerprinting is yielding important insights into the epidemiology of Mycobacterium bovis infections. Various genetic markers used in restriction fragment length polymorphism (RFLP) have recently been exploited for fingerprinting of M. bovis isolates. The newly developed spacer oligonucleotide typing aimed to investigate the polymorphism of M. tuberculosis in the DR locus, has also been applied to the molecular typing of M. bovis isolates. This work compared the performance of the insertion sequence (IS) IS6110, IS1081 and the genetic elements polymorphic G + C-rich repeat (PGRS) and direct repeat (DR) used in RFLP analysis with spoligotyping using a group of 128 Spanish M. bovis isolat… Leer más

Aranaz A., Liebana E., Mateos A., Dominguez L. and Cousins DV.

April 1st, 1998

Corynebacterium camporealensis sp. nov., associated with subclinical mastitis in sheep

International Journal Of Systematic Bacteriology 48(Pt 2):463-8.

     Four strains of a hitherto-unknown catalase-positive, facultatively anaerobic Corynebacterium species were isolated from the milk of sheep affected by subclinical mastitis. The most characteristic phenotypic reactions of the four strains were their weak fermentative acid production from glucose, their failure to produce acid from mannitol, xylose, sucrose and maltose, and a strong CAMP reaction with Staphylococcus aureus. Chemotaxonomic investigations revealed the presence of a cell wall based on meso-diaminopimelic acid and short-chain mycolic acids, which is consistent with the genus Corynebacterium. A comparative 16S rRNA gene sequence analysis confirmed that the organisms from sheep were members of the genus Corynebacterium, where they … Leer más

Fernandez-Garayzabal JF., Collins MD., Hutson RA., Gonzalez I., Fernandez E. and Dominguez L..

February 15th, 1998

Comparison of four tests to evaluate the reactivity of rabbit sera against envelope or Gag-related proteins of bovine leukemia virus (BLV)

Veterinary Microbiology 60(1):13-25.

     Bovine leukemia virus (BLV) has a long latency period during which animals are inapparently infected, may spread the disease, and are only detected by serological techniques or by the most cumbersome molecular biology techniques. We have compared techniques for detecting either total antibodies (ELISA), anti-p24 and Gag-related proteins (Western blot), or anti-gp51 (agar gel immunodiffusion, AGID, and syncytia inhibition, SI) in rabbits inoculated experimentally with inocula of variable immunogenicity. The two tests to detect antibodies to gp51 correlated well in sera clearly positive or clearly negative by either one, but correlation was poor in the intermediate groups. All sera positive by AGID were also positive by ELISA, but results did… Leer más

Domenech A., Llames L., Goyache J., Suarez G. and Gomez-Lucia E.

January 1st, 1998

Allele-specific PCR method based on pncA and oxyR sequences for distinguishing Mycobacterium bovis from Mycobacterium tuberculosis: intraspecific M. bovis pncA sequence polymorphism

Journal Of Clinical Microbiology 36(1):239-42.

     An allele-specific amplification method based on two genetic polymorphisms to differentiate Mycobacterium tuberculosis from Mycobacterium bovis was tested. Based on the differences found at position 169 in the pncA genes from M. tuberculosis and M. bovis, a PCR system which was able to differentiate most of the 237 M. tuberculosis complex isolates tested in one of the two species was developed. All 121 M. tuberculosis strains showed the expected base (cytosine) at position 169. Most of the M. bovis isolates had a guanine at the cited position. Nevertheless, 18 of the 116 M. bovis isolates, all of them goat isolates, showed the pncA polymorphism specific to M. tuberculosis. These results suggest that goat M. bovis may be the nicotinamidase-m… Leer más

Espinosa LE., Galan JC., Gutierrez M., Samper S., Garcia-Marin JF., Martin C., Dominguez L., de Rafael L., Baquero F., Gomez-Mampaso E. and Blazquez J.

January 1st, 1998

Evaluation of the gamma-interferon assay for eradication of tuberculosis in a goat herd

Australian Veterinary Journal 76(1):50-3.

     OBJECTIVE: To evaluate the usefulness of the gamma-interferon assay in the diagnosis of caprine tuberculosis in comparison with a single intradermal tuberculin test, and to obtain a group of animals free from this infection in a herd with a high prevalence. DESIGN: An immunological study involving four serial comparative gamma-interferon and single intradermal tuberculin tests. ANIMALS: A herd of 87 goats of Guadarrama breed. PROCEDURE: Serial testing and segregation of animals. RESULTS: We found that the number of infections detected by the gamma-interferon test was considerably greater than the number detected by the single intradermal tuberculin test. A group of 10 animals was negative to both tests in two consecutive rounds and three ki… Leer más

Liebana E., Aranaz A., Urquia JJ., Mateos A. and Dominguez L..

January 1st, 1998

Evaluation of four DNA typing techniques in epidemiological investigations of bovine tuberculosis

Journal Of Clinical Microbiology 36(1):168-78.

     DNA fingerprinting techniques were used to type 273 isolates of Mycobacterium bovis from Australia, Canada, the Republic of Ireland, and Iran. The results of restriction fragment length polymorphism (RFLP) analysis with DNA probes from IS6110, the direct repeat (DR), and the polymorphic GC-rich sequence (PGRS) were compared with those of a new PCR-based method called spacer oligonucleotide typing (spoligotyping) developed for the rapid typing of Mycobacterium tuberculosis (J. Kamerbeek et al., J. Clin. Microbiol. 35:907-914, 1997). Eighty-five percent of the isolates harbored a single copy of IS6110, and 81.5% of these carried IS6110 on the characteristic 1.9-kb restriction fragment. RFLP analysis with IS6110 identified 23 different types, … Leer más

Cousins DV., Williams S., Liebana E., Aranaz A., Bunschotten A., Van Emnden JDA. and Ellis T.

Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ZTA 250 SUAT 61 SUAT 15
2 ICM 237 ZTA 53 MYC 11
3 MYC 227 MYC 48 SAP 11
4 SUAT 219 ICM 40 ZTA 6
5 NED 75 SAP 31 ICM 4
  VISAVET 1124 315 55

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.907 ZTA 5.359 ZTA 6.183
2 SAP 3.818 SUAT 4.227 SAP 6.06
3 SUAT 2.987 SAP 4.095 NED 4.3
4 MYC 2.8 NED 3.858 SUAT 4.287
5 DICM 2.776 ICM 3.798 MYC 3.118
VISAVET 3.284 4.885 6.794

ISI Scientific Publications
Total Last 60 mo.
1 Transbound Emerg Dis 93 Front Vet Sci 35
2 Vet Microbiol 88 Transbound Emerg Dis 32
3 Prev Vet Med 77 Animals 15
4 Front Vet Sci 61 Res Vet Sci 14
5 PLoS ONE 56 Sci Rep 14
  All journals 354   All journals 39