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SCI scientific publications 2006

November 1st, 2006

Molecular typing and anti-microbial susceptibility of clinical isolates of Streptococcus equi ssp. zooepidemicus from equine bacterial endometritis

Journal Of Veterinary Medicine. B, Infectious Diseases And Veterinary Public Health 53(9):451-4.

     The anti-microbial susceptibility and genetic diversity of 65 strains of Streptococcus equi ssp. zooepidemicus (Sez) isolated from mares presenting clinical signs of endometritis was determined by disk agar diffusion and pulsed field gel electrophoresis (PFGE) methods, respectively. Overall, Sez isolates were susceptible to beta-lactams, enrofloxacin, trimethoprim-sulfamethoxazole and gentamicin. These anti-microbials could be recommended as empiric anti-microbial therapy in cases of endometritis caused by Sez. Pulsed field gel electrophoresis typing revealed a great genetic diversity (56 different PFGE macrorestriction profiles) and a low level of genetic relatedness amongst the isolates… Leer más

Luque I., Fernandez-Garayzabal JF., Blume V., Maldonado A., Astorga R. and Tarradas C.

November 1st, 2006

Pseudomonas simiae sp. nov., isolated from clinical specimens from monkeys (Callithrix geoffroyi)

International Journal Of Systematic And Evolutionary Microbiology 56(Pt 11):2671-6.

     An unusual Gram-negative, catalase- and oxidase-positive, rod-shaped bacterium isolated from different clinical samples from two monkeys (Callithrix geoffroyi) was characterized by phenotypic and molecular genetic methods. The micro-organism was tentatively identified as a Pseudomonas species on the basis of the results of cellular morphological and biochemical tests. Fatty acid studies confirmed this generic placement and comparative 16S rRNA gene sequencing studies demonstrated that the unknown isolates were phylogenetically closely related to each other (100% sequence similarity) and were part of the `Pseudomonas fluorescens intrageneric cluster`. The novel bacterium, however, was distinguished from other phylogenetically related species… Leer más

Vela AI., Gutierrez M., Falsen E., Rollan E., Simarro I., Garcia P., Dominguez L., Ventosa A. and Fernandez-Garayzabal JF..

October 1st, 2006

Detection and characterization of extended-spectrum beta-lactamases in Salmonella enterica strains of healthy food animals in Spain

The Journal Of Antimicrobial Chemotherapy 58(4):844-7.

     OBJECTIVES: To carry out the characterization of the genes encoding extended-spectrum beta-lactamases (ESBLs) and their genetic environments in four expanded-spectrum cephalosporin-resistant Salmonella enterica isolates (serovars: two Virchow, one Enteritidis, one Rissen) recovered during the monitoring programmes performed in Spain by the VAV Network from faecal samples of pigs, poultry and laying hens at the slaughterhouse level. METHODS: The presence and characterization of ESBL genes as well as their genetic environments in the four S. enterica isolates were investigated by PCR and sequencing. The presence of other resistance genes was also analysed by PCR and sequencing. RESULTS: Three avian S. enterica isolates (two Virchow and one En… Leer más

Riano I., Moreno MA., Teshager T., Dominguez L. and Torres C.

October 1st, 2006

Clinical and molecular analysis of macrolide resistance in Mycobacterium avium complex lung disease

American Journal Of Respiratory And Critical Care Medicine 174(8):928-34.

     RATIONALE: The clinical features and outcome of macrolide-resistant Mycobacterium avium complex (MAC) lung disease are not known. OBJECTIVES: Characterize patients, treatment, and isolates in macrolide-resistant MAC lung disease. METHODS: Retrospective chart review, susceptibility testing, molecular fingerprinting, and DNA sequence analyses of resistant MAC isolates. MEASUREMENTS AND MAIN RESULTS: We identified 51 patients over a 15-yr period with clarithromycin-resistant MAC (minimum inhibitory concentration (MIC)>or=32 microg/ml) lung disease at a single referral center. Twenty-four (47%) patients had nodular disease with bronchiectasis and 27 (53%) had upper lobe cavitary disease. Most patients (77%) had M. intracellulare. Sequencing of … Leer más

Griffith DE., Brown-Elliott BA., Langsjoen B., Zhang Y., Pan X., Girard W., Nelson K., Caccitolo J., Alvarez J., Shepherd S., Willson R., Graviss EA. and Wallace RJ JR.

September 1st, 2006

Comparison of four different culture media for isolation and growth of type II and type I/III Mycobacterium avium subsp. paratuberculosis strains isolated from cattle and goats

Applied And Environmental Microbiology 72(9):5927-32.

     Culture is considered the definitive technique for Johne`s disease diagnosis, and it is essential for later applications of certain molecular typing techniques. In this study, we have tested four solid media (Herrold`s egg yolk medium [HEYM] with sodium pyruvate and mycobactin [HEYMm-SP], HEYM with mycobactin and without sodium pyruvate [HEYMm], Middlebrook 7H11 with mycobactin [Mm], and Läwenstein-Jensen with mycobactin [LJm]) for isolation of Mycobacterium avium subsp. paratuberculosis strains in 319 tissue samples from cattle herds and goat flocks. We have shown that each of the two main groups of M. avium subsp. paratuberculosis (type II and type I/III) has different requirements for growth in the culture media studied. The recommended … Leer más

de Juan L., Alvarez J., Romero B., Bezos J., Castellanos E., Aranaz A., Mateos A. and Dominguez L..

September 1st, 2006

Molecular epidemiology of multidrug-resistant Mycobacterium bovis isolates with the same spoligotyping profile as isolates from animals

Journal Of Clinical Microbiology 44(9):3405-8.

     PCR-based characterization techniques have been adopted in most laboratories for Mycobacterium bovis typing. We report a molecular characterization of human multidrug-resistant M. bovis isolates and three bovine isolates that share the spoligotyping profile. The analysis of the direct repeat region showed that both groups differed in the presence of spacers not included in the current membrane. They were also distinguished by two out of the nine mycobacterial interspersed repetitive unit variable-number tandem repeat loci tested, indicating that the human infection was not acquired from the cattle from which isolates were obtained. These results highlight that a combination of techniques is required for appropriate discrimination, even for … Leer más

Romero B., Aranaz A., de Juan L., Alvarez J., Bezos J., Mateos A., Gomez-Mampaso E. and Dominguez L..

July 1st, 2006

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis

Veterinary Research 37(4):593-606.

     The intradermal tuberculin (IDTB) test and the interferon-gamma (IFN-gamma) assay are used worldwide for detection of bovine tuberculosis in cattle, but little is known about the effect of co-infecting agents on the performance of these diagnostic tests. This report describes a field trial conducted in a cattle herd with dual infection (bovine tuberculosis and paratuberculosis) during 3.5 years. It has been based on a strategic approach encompassing serial parallel testing (comparative IDTB test, the IFN-gamma assay and serology of paratuberculosis) that was repeated 8 times over the period, and segregation of animals into two herds. The IDTB test detected 65.2% and the IFN-gamma test detected 69.6% of the Mycobacterium bovis culture-positi… Leer más

Aranaz A., de Juan L., Bezos J., Alvarez J., Romero B., Lozano F., Paramio JL., Lopez-Sanchez J., Mateos A. and Dominguez L..

June 15th, 2006

Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattle

Veterinary Microbiology 115(1-3):102-10.

     Molecular characterization of Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) isolates classifies them into three groups: cattle or Type II, sheep or Type I, and intermediate or Type III. To avoid problems associated with characterization of extremely slow growth strains, PCR-based techniques that divide the M. a. paratuberculosis strains in two main groups (cattle or Type II, and sheep or Types I/III) can be performed. The objectives of this study were to characterize the M. a. paratuberculosis isolates identified by different PCR-based tests (IS1311-PCR and restriction endonuclease analysis, PCR test based on a DNA sequence difference, and a PCR aimed at three Type I-specific loci), and to determine the clinical and e… Leer más

de Juan L., Alvarez J., Aranaz A., Rodriguez-Bertos A., Romero B., Bezos J., Mateos A. and Dominguez L..

June 1st, 2006

Isolation of Corynebacterium xerosis from animal clinical specimens

Journal Of Clinical Microbiology 44(6):2242-3.

     This article describes the first identification of Corynebacterium xerosis from animal clinical specimens, which was confirmed by microbiological and molecular genetic (16S rRNA gene sequencing) methods… Leer más

Vela AI., Gracia E., Fernandez A., Dominguez L. and Fernandez-Garayzabal JF..

April 1st, 2006

R1940 Beta-lactam resistance in Haemophilus parasuis

Clinical Microbiology And Infection 12(4):S635-S767.

     Objectives: Haemophilus parasuis is a commensal organism of the upper respiratory tract of conventional pigs that, under appropriate conditions, can cause the Glasser disease, characterized by severe systemic infection, fibrinous polyserositis, arthritis and meningitis. This is an emerging infection with increasing importance in swine production. In our laboratory, we are detecting an increase in the prevalence of resistance to beta-lactam antibiotics in H. parasuis, being this antimicrobial family the first clinical election against respiratory tract infections in swine. Resistance to beta-lactam antimicrobials has yet not been described nor characterized in H. parasuis. Methods: Antimicrobial susceptibility test and MICs were performed fo… Leer más

San Millan A., Escudero JA., Catalan A., Porrero MC., Dominguez L., Moreno MA. and Gonzalez-Zorn B..

March 1st, 2006

Unexpected inefficiency of the European pharmacopoeia sterility test for detecting contamination in clostridial vaccines

Vaccine 24(10):1710-15.

     Three outbreaks of clostridial disease in ruminants were reported in Spain. Out of 202,525 animals in affected herds, 41,767 were infected and 22,189 died. Epidemiological investigation linked these outbreaks with vaccination with three different commercial anti-clostridial vaccines contaminated with Clostridium sordellii. Vaccines were produced by the same manufacturer. Microbiological and molecular genetic analyses confirmed this association, and isolates of C. sordellii with identical biochemical and pulsed-field gel electrophoresis (PFGE) macrorestriction patterns were isolated from both diseased animals and the epidemiologically related vaccines. Contamination of the commercial vaccines was not detected by the sterility test proposed b… Leer más

Tellez S., Casimiro R., Vela AI., Fernandez-Garayzabal JF., Ezquerra R., Latre MV., Briones V., Goyache J., Bullido R., Arboix M. and Dominguez L..

March 1st, 2006

Antigenic properties and diagnostic potential of African swine fever virus protein pp62 expressed in insect cells

Journal Of Clinical Microbiology 44(3):950-6.

     African swine fever (ASF) is an infectious and economically important disease of domestic pigs. The absence of vaccine renders the diagnostic test the only tool that can be used for the control of new outbreaks of the disease. At present, the enzyme-linked immunosorbent assay (ELISA) test is the most useful method for large-scale ASF serological studies, although false positives have been detected, mainly on poorly preserved sera. In order to improve the current diagnostic test available for ASF, we have studied the antigenic properties of the ASF virus polyprotein pp62 and its suitability for use in a novel ELISA. Two well-known antigenic proteins of ASF virus, p32 and p54, were also included in this study. These proteins were expressed in… Leer más

Gallardo C., Blanco E., Rodriguez JM., Carrascosa AL. and Sanchez-Vizcaino JM..

February 1st, 2006

Neonatal mortality in puppies due to bacteremia by Streptococcus dysgalactiae subsp. dysgalactiae

Journal Of Clinical Microbiology 44(2):666-8.

     We report a case of bacteremia in puppies caused by Streptococcus dysgalactiae subsp. dysgalactiae. Identification was achieved by phenotypic and molecular genetic methods. This is the first report of the recovery of S. dysgalactiae subsp. dysgalactiae from dogs… Leer más

Vela AI., Falsen E., Simarro I., Rollan E., Collins MD., Dominguez L. and Fernandez-Garayzabal JF..

January 1st, 2006

A spontaneous genomic deletion in Listeria ivanovii identifies LIPI-2, a species-specific pathogenicity island encoding sphingomyelinase and numerous internalins

Molecular Microbiology 59(2):415-32.

     Listeria ivanovii differs from the human pathogen Listeria monocytogenes in that it specifically affects ruminants, causing septicaemia and abortion but not meningo-encephalitis. The genetic characterization of spontaneous L. ivanovii mutants lacking the virulence factor SmcL (sphingomyelinase) led us to identify LIPI-2, the first species-specific pathogenicity island from Listeria. Besides SmcL, this 22 kb chromosomal locus encodes 10 internalin (Inl) proteins: i-InlB1 and -B2 are large/surface-associated Inls similar to L. monocytogenes InlB; i-InlE to -L are small/excreted (SE)-Inls, i-InlG being a tandem fusion of two SE-Inls. Except i-inlB1, all LIPI-2 inl genes are controlled by the virulence regulator, PrfA. LIPI-2 is inserted into a… Leer más

Dominguez-Bernal G., Muller-Altrock S., Gonzalez-Zorn B., Scortti M., Herrmann P., Monzo HJ., Lacharme L., Kreft J. and Vazquez-Boland JA.

Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ZTA 250 SUAT 61 SUAT 15
2 ICM 237 ZTA 53 MYC 11
3 MYC 227 MYC 48 SAP 11
4 SUAT 219 ICM 40 ZTA 6
5 NED 75 SAP 31 ICM 4
  VISAVET 1124 315 55

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.907 ZTA 5.359 ZTA 6.183
2 SAP 3.818 SUAT 4.227 SAP 6.06
3 SUAT 2.987 SAP 4.095 NED 4.3
4 MYC 2.8 NED 3.858 SUAT 4.287
5 DICM 2.776 ICM 3.798 MYC 3.118
VISAVET 3.284 4.885 6.794

ISI Scientific Publications
Total Last 60 mo.
1 Transbound Emerg Dis 93 Front Vet Sci 35
2 Vet Microbiol 88 Transbound Emerg Dis 32
3 Prev Vet Med 77 Animals 15
4 Front Vet Sci 61 Res Vet Sci 14
5 PLoS ONE 56 Sci Rep 14
  All journals 354   All journals 39