Publicaciones científicas SCI 2006
Molecular typing and anti-microbial susceptibility of clinical isolates of Streptococcus equi ssp. zooepidemicus from equine bacterial endometritis
Journal Of Veterinary Medicine. B, Infectious Diseases And Veterinary Public Health 53(9):451-4.
The anti-microbial susceptibility and genetic diversity of 65 strains of Streptococcus equi ssp. zooepidemicus (Sez) isolated from mares presenting clinical signs of endometritis was determined by disk agar diffusion and pulsed field gel electrophoresis (PFGE) methods, respectively. Overall, Sez isolates were susceptible to beta-lactams, enrofloxacin, trimethoprim-sulfamethoxazole and gentamicin. These anti-microbials could be recommended as empiric anti-microbial therapy in cases of endometritis caused by Sez. Pulsed field gel electrophoresis typing revealed a great genetic diversity (56 different PFGE macrorestriction profiles) and a low level of genetic relatedness amongst the isolates… Leer más
Luque I., Fernandez-Garayzabal JF., Blume V., Maldonado A., Astorga R. y Tarradas C.
Pseudomonas simiae sp. nov., isolated from clinical specimens from monkeys (Callithrix geoffroyi)
International Journal Of Systematic And Evolutionary Microbiology 56(Pt 11):2671-6.
An unusual Gram-negative, catalase- and oxidase-positive, rod-shaped bacterium isolated from different clinical samples from two monkeys (Callithrix geoffroyi) was characterized by phenotypic and molecular genetic methods. The micro-organism was tentatively identified as a Pseudomonas species on the basis of the results of cellular morphological and biochemical tests. Fatty acid studies confirmed this generic placement and comparative 16S rRNA gene sequencing studies demonstrated that the unknown isolates were phylogenetically closely related to each other (100% sequence similarity) and were part of the `Pseudomonas fluorescens intrageneric cluster`. The novel bacterium, however, was distinguished from other phylogenetically related species… Leer más
Vela AI., Gutierrez M., Falsen E., Rollan E., Simarro I., Garcia P., Dominguez L., Ventosa A. y Fernandez-Garayzabal JF..
Detection and characterization of extended-spectrum beta-lactamases in Salmonella enterica strains of healthy food animals in Spain
The Journal Of Antimicrobial Chemotherapy 58(4):844-7.
OBJECTIVES: To carry out the characterization of the genes encoding extended-spectrum beta-lactamases (ESBLs) and their genetic environments in four expanded-spectrum cephalosporin-resistant Salmonella enterica isolates (serovars: two Virchow, one Enteritidis, one Rissen) recovered during the monitoring programmes performed in Spain by the VAV Network from faecal samples of pigs, poultry and laying hens at the slaughterhouse level. METHODS: The presence and characterization of ESBL genes as well as their genetic environments in the four S. enterica isolates were investigated by PCR and sequencing. The presence of other resistance genes was also analysed by PCR and sequencing. RESULTS: Three avian S. enterica isolates (two Virchow and one En… Leer más
Riano I., Moreno MA., Teshager T., Dominguez L. y Torres C.
Clinical and molecular analysis of macrolide resistance in Mycobacterium avium complex lung disease
American Journal Of Respiratory And Critical Care Medicine 174(8):928-34.
RATIONALE: The clinical features and outcome of macrolide-resistant Mycobacterium avium complex (MAC) lung disease are not known. OBJECTIVES: Characterize patients, treatment, and isolates in macrolide-resistant MAC lung disease. METHODS: Retrospective chart review, susceptibility testing, molecular fingerprinting, and DNA sequence analyses of resistant MAC isolates. MEASUREMENTS AND MAIN RESULTS: We identified 51 patients over a 15-yr period with clarithromycin-resistant MAC (minimum inhibitory concentration (MIC)>or=32 microg/ml) lung disease at a single referral center. Twenty-four (47%) patients had nodular disease with bronchiectasis and 27 (53%) had upper lobe cavitary disease. Most patients (77%) had M. intracellulare. Sequencing of … Leer más
Griffith DE., Brown-Elliott BA., Langsjoen B., Zhang Y., Pan X., Girard W., Nelson K., Caccitolo J., Alvarez J., Shepherd S., Willson R., Graviss EA. y Wallace RJ JR.
Comparison of four different culture media for isolation and growth of type II and type I/III Mycobacterium avium subsp. paratuberculosis strains isolated from cattle and goats
Applied And Environmental Microbiology 72(9):5927-32.
Culture is considered the definitive technique for Johne`s disease diagnosis, and it is essential for later applications of certain molecular typing techniques. In this study, we have tested four solid media (Herrold`s egg yolk medium [HEYM] with sodium pyruvate and mycobactin [HEYMm-SP], HEYM with mycobactin and without sodium pyruvate [HEYMm], Middlebrook 7H11 with mycobactin [Mm], and Läwenstein-Jensen with mycobactin [LJm]) for isolation of Mycobacterium avium subsp. paratuberculosis strains in 319 tissue samples from cattle herds and goat flocks. We have shown that each of the two main groups of M. avium subsp. paratuberculosis (type II and type I/III) has different requirements for growth in the culture media studied. The recommended … Leer más
de Juan L., Alvarez J., Romero B., Bezos J., Castellanos E., Aranaz A., Mateos A. y Dominguez L..
Molecular epidemiology of multidrug-resistant Mycobacterium bovis isolates with the same spoligotyping profile as isolates from animals
Journal Of Clinical Microbiology 44(9):3405-8.
PCR-based characterization techniques have been adopted in most laboratories for Mycobacterium bovis typing. We report a molecular characterization of human multidrug-resistant M. bovis isolates and three bovine isolates that share the spoligotyping profile. The analysis of the direct repeat region showed that both groups differed in the presence of spacers not included in the current membrane. They were also distinguished by two out of the nine mycobacterial interspersed repetitive unit variable-number tandem repeat loci tested, indicating that the human infection was not acquired from the cattle from which isolates were obtained. These results highlight that a combination of techniques is required for appropriate discrimination, even for … Leer más
Romero B., Aranaz A., de Juan L., Alvarez J., Bezos J., Mateos A., Gomez-Mampaso E. y Dominguez L..
Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis
Veterinary Research 37(4):593-606.
The intradermal tuberculin (IDTB) test and the interferon-gamma (IFN-gamma) assay are used worldwide for detection of bovine tuberculosis in cattle, but little is known about the effect of co-infecting agents on the performance of these diagnostic tests. This report describes a field trial conducted in a cattle herd with dual infection (bovine tuberculosis and paratuberculosis) during 3.5 years. It has been based on a strategic approach encompassing serial parallel testing (comparative IDTB test, the IFN-gamma assay and serology of paratuberculosis) that was repeated 8 times over the period, and segregation of animals into two herds. The IDTB test detected 65.2% and the IFN-gamma test detected 69.6% of the Mycobacterium bovis culture-positi… Leer más
Aranaz A., de Juan L., Bezos J., Alvarez J., Romero B., Lozano F., Paramio JL., Lopez-Sanchez J., Mateos A. y Dominguez L..
Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattle
Veterinary Microbiology 115(1-3):102-10.
Molecular characterization of Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) isolates classifies them into three groups: cattle or Type II, sheep or Type I, and intermediate or Type III. To avoid problems associated with characterization of extremely slow growth strains, PCR-based techniques that divide the M. a. paratuberculosis strains in two main groups (cattle or Type II, and sheep or Types I/III) can be performed. The objectives of this study were to characterize the M. a. paratuberculosis isolates identified by different PCR-based tests (IS1311-PCR and restriction endonuclease analysis, PCR test based on a DNA sequence difference, and a PCR aimed at three Type I-specific loci), and to determine the clinical and e… Leer más
de Juan L., Alvarez J., Aranaz A., Rodriguez-Bertos A., Romero B., Bezos J., Mateos A. y Dominguez L..
Isolation of Corynebacterium xerosis from animal clinical specimens
Journal Of Clinical Microbiology 44(6):2242-3.
This article describes the first identification of Corynebacterium xerosis from animal clinical specimens, which was confirmed by microbiological and molecular genetic (16S rRNA gene sequencing) methods… Leer más
Vela AI., Gracia E., Fernandez A., Dominguez L. y Fernandez-Garayzabal JF..
R1940 Beta-lactam resistance in Haemophilus parasuis
Clinical Microbiology And Infection 12(4):S635-S767.
Objectives: Haemophilus parasuis is a commensal organism of the upper respiratory tract of conventional pigs that, under appropriate conditions, can cause the Glasser disease, characterized by severe systemic infection, fibrinous polyserositis, arthritis and meningitis. This is an emerging infection with increasing importance in swine production. In our laboratory, we are detecting an increase in the prevalence of resistance to beta-lactam antibiotics in H. parasuis, being this antimicrobial family the first clinical election against respiratory tract infections in swine. Resistance to beta-lactam antimicrobials has yet not been described nor characterized in H. parasuis. Methods: Antimicrobial susceptibility test and MICs were performed fo… Leer más
San Millan A., Escudero JA., Catalan A., Porrero MC., Dominguez L., Moreno MA. y Gonzalez-Zorn B..
Unexpected inefficiency of the European pharmacopoeia sterility test for detecting contamination in clostridial vaccines
Vaccine 24(10):1710-15.
Three outbreaks of clostridial disease in ruminants were reported in Spain. Out of 202,525 animals in affected herds, 41,767 were infected and 22,189 died. Epidemiological investigation linked these outbreaks with vaccination with three different commercial anti-clostridial vaccines contaminated with Clostridium sordellii. Vaccines were produced by the same manufacturer. Microbiological and molecular genetic analyses confirmed this association, and isolates of C. sordellii with identical biochemical and pulsed-field gel electrophoresis (PFGE) macrorestriction patterns were isolated from both diseased animals and the epidemiologically related vaccines. Contamination of the commercial vaccines was not detected by the sterility test proposed b… Leer más
Tellez S., Casimiro R., Vela AI., Fernandez-Garayzabal JF., Ezquerra R., Latre MV., Briones V., Goyache J., Bullido R., Arboix M. y Dominguez L..
Antigenic properties and diagnostic potential of African swine fever virus protein pp62 expressed in insect cells
Journal Of Clinical Microbiology 44(3):950-6.
African swine fever (ASF) is an infectious and economically important disease of domestic pigs. The absence of vaccine renders the diagnostic test the only tool that can be used for the control of new outbreaks of the disease. At present, the enzyme-linked immunosorbent assay (ELISA) test is the most useful method for large-scale ASF serological studies, although false positives have been detected, mainly on poorly preserved sera. In order to improve the current diagnostic test available for ASF, we have studied the antigenic properties of the ASF virus polyprotein pp62 and its suitability for use in a novel ELISA. Two well-known antigenic proteins of ASF virus, p32 and p54, were also included in this study. These proteins were expressed in… Leer más
Gallardo C., Blanco E., Rodriguez JM., Carrascosa AL. y Sanchez-Vizcaino JM..
Neonatal mortality in puppies due to bacteremia by Streptococcus dysgalactiae subsp. dysgalactiae
Journal Of Clinical Microbiology 44(2):666-8.
We report a case of bacteremia in puppies caused by Streptococcus dysgalactiae subsp. dysgalactiae. Identification was achieved by phenotypic and molecular genetic methods. This is the first report of the recovery of S. dysgalactiae subsp. dysgalactiae from dogs… Leer más
Vela AI., Falsen E., Simarro I., Rollan E., Collins MD., Dominguez L. y Fernandez-Garayzabal JF..
A spontaneous genomic deletion in Listeria ivanovii identifies LIPI-2, a species-specific pathogenicity island encoding sphingomyelinase and numerous internalins
Molecular Microbiology 59(2):415-32.
Listeria ivanovii differs from the human pathogen Listeria monocytogenes in that it specifically affects ruminants, causing septicaemia and abortion but not meningo-encephalitis. The genetic characterization of spontaneous L. ivanovii mutants lacking the virulence factor SmcL (sphingomyelinase) led us to identify LIPI-2, the first species-specific pathogenicity island from Listeria. Besides SmcL, this 22 kb chromosomal locus encodes 10 internalin (Inl) proteins: i-InlB1 and -B2 are large/surface-associated Inls similar to L. monocytogenes InlB; i-InlE to -L are small/excreted (SE)-Inls, i-InlG being a tandem fusion of two SE-Inls. Except i-inlB1, all LIPI-2 inl genes are controlled by the virulence regulator, PrfA. LIPI-2 is inserted into a… Leer más
Dominguez-Bernal G., Muller-Altrock S., Gonzalez-Zorn B., Scortti M., Herrmann P., Monzo HJ., Lacharme L., Kreft J. y Vazquez-Boland JA.
