Publicaciones científicas 2005

Vancomycin-resistant Enterococcus faecium clone in swine, Europe

Emerging Infectious Diseases 11(12):1985-7.

Novais C., Coque TM., Boerlin P., Herrero IA., Moreno MA., Dominguez L. y Peixe L.

Characterization of Mycobacterium caprae isolates from Europe by mycobacterial interspersed repetitive unit genotyping

Journal Of Clinical Microbiology 43(10):4984-92.

     Mycobacterium caprae, a recently defined member of the Mycobacterium tuberculosis complex, causes tuberculosis among animals and, to a limited extent, in humans in several European countries. To characterize M. caprae in comparison with other Mycobacterium tuberculosis complex members and to evaluate genotyping methods for this species, we analyzed 232 M. caprae isolates by mycobacterial interspersed repetitive unit (MIRU) genotyping and by spoligotyping. The isolates originated from 128 distinct epidemiological settings in 10 countries, spanning a period of 25 years. We found 78 different MIRU patterns (53 unique types and 25 clusters with group sizes from 2 to 9) but only 17 spoligotypes, giving Hunter-Gaston discriminatory indices of 0.9… Leer más

Prodinger WM., Brandstatter A., Naumann N., Pacciarini M., Kubica T., Boschiroli ML., Aranaz A., Nagy G., Cvetnic Z., Ocepek M., Skrypnyk A., Erler W., Niemann S., Pavlik I. y Moser I.

Genetic basis for dissemination of armA

The Journal Of Antimicrobial Chemotherapy 56(3):583-5.

     OBJECTIVES AND METHODS: armA is a novel plasmid-borne 16S rRNA methyltransferase that confers high-level resistance to 4,6-disubstituted deoxystreptamines. Recently, we have isolated from a high-level broad-spectrum aminoglycoside-resistant Escherichia coli animal isolate a plasmid, pMUR050, that bore the armA gene. In order to elucidate the genetic basis for the spread of armA, we have determined the complete nucleotide sequence of pMUR050. RESULTS: armA was borne by a complex transposon composite flanked by two direct repeats of IS26. The transposon composite included a class one integron with sul1 for resistance to sulphonamides and ant3``9 conferring resistance to spectinomycin-streptomycin, and a macrolide efflux pump and mefE/mel conf… Leer más

Gonzalez-Zorn B., Catalan A., Escudero JA., Dominguez L., Teshager T., Porrero MC. y Moreno MA.

Occurrence and relatedness of vancomycin-resistant enterococci in animals, humans, and the environment in different European regions

Applied And Environmental Microbiology 71(9):5383-90.

     Vancomycin-resistant enterococci (VRE) in Europe are thought to have emerged partly due to the use of the glycopeptide avoparcin in animal husbandry. We compared the occurrence of VRE in geographical regions of Europe in which until 1997 large amounts of avoparcin were used (Spain, United Kingdom, and Denmark) with the occurrence of VRE in Sweden, where avoparcin was banned in 1986. We also studied the relatedness between VRE strains from different regions and habitats. In total, 2,580 samples were collected from humans, animals, and the environment (soil, sewage, recipient water). VRE resistant to 20 microg/ml vancomycin were identified in 8.2% of the samples and were found most frequently in raw and treated urban sewage samples (means, 71… Leer más

Kuhn I., Iversen A., Finn M., Greko C., Burman LG., Blanch AR., Vilanova X., Manero A., Taylor H., Caplin J., Dominguez L., Herrero IA., Moreno MA. y Mollby R.

armA and aminoglycoside resistance in Escherichia coli

Emerging Infectious Diseases 11(6):954-6.

     We report armA in an Escherichia coli pig isolate from Spain. The resistance gene was borne by self-transferable IncN plasmid pMUR050. Molecular analysis of the plasmid and of the armA locus confirmed the spread of this resistance determinant… Leer más

Gonzalez-Zorn B., Teshager T., Casas MC., Porrero MC., Moreno MA., Courvalin P. y Dominguez L..

Genetic diversity of Mycobacterium avium subspecies paratuberculosis isolates from goats detected by pulsed-field gel electrophoresis

Veterinary Microbiology 106(3-4):249-57.

     Paratuberculosis in goats occurs worldwide causing considerable economic losses mainly due to reduced milk production. Nowadays, there is still relatively little knowledge about the epidemiology of this disease in goats, and only a few epidemiological studies have been carried out in goats naturally infected with Mycobacterium avium subspecies paratuberculosis (M. a. paratuberculosis). The objective of this study was to characterize forty four clinical caprine isolates of M. a. paratuberculosis by different molecular techniques (pulsed-field gel electrophoresis [PFGE], restriction fragment length polymorphism analysis coupled with hybridization to IS900, and IS1311 polymerase chain reaction-restriction enzyme analysis) to determine the most… Leer más

de Juan L., Mateos A., Dominguez L., Sharp JM. y Stevenson K.

Bacterial and host factors implicated in nasal carriage of methicillin-resistant Staphylococcus aureus in mice

Infection And Immunity 73(3):1847-51.

     Nasal carriage is a major risk factor for Staphylococcus aureus infection, especially for methicillin-resistant strains (MRSA). Using a mouse model of nasal carriage, we have compared several S. aureus strains and demonstrated increased colonization levels by MRSA in cystic fibrosis transmembrane conductance regulator-deficient mice and Toll-like receptor 2 (TLR2)-deficient mice but not TLR4-deficient mice… Leer más

Gonzalez-Zorn B., Senna JP., Fiette L., Shorte S., Testard A., Chignard M., Courvalin P. y Grillot-Courvalin C.

Uruburuella suis gen. nov., sp. nov., isolated from clinical specimens of pigs

International Journal Of Systematic And Evolutionary Microbiology 55(Pt 2):643-7.

     Five strains of an unusual Gram-negative, catalase-positive, oxidase-positive, coccobacillus-shaped bacterium isolated from the lungs and heart of pigs with pneumonia and pericarditis were characterized by phenotypic and molecular genetic methods. On the basis of cellular morphology and biochemical criteria, the isolates were tentatively assigned to the family Neisseriaceae, although they did not appear to correspond to any recognized genus or species. Comparative 16S rRNA gene sequencing showed that the five unidentified strains were phylogenetically highly related to each other and represent a hitherto unknown subline within the family Neisseriaceae. On the basis of both phenotypic and phylogenetic evidence, it is proposed that the unknow… Leer más

Vela AI., Collins MD., Lawson P., Garcia N., Dominguez L. y Fernandez-Garayzabal JF..

Monitoring and characterization of extended-spectrum beta-lactamases in Escherichia coli strains from healthy and sick animals in Spain in 2003

Antimicrobial Agents And Chemotherapy 49(3):1262-4.

     Genes encoding CTX-M-14, CTX-M-9, CTX-M-1, CTX-M-32, SHV-12, TEM-52, or CMY-2 beta-lactamases were detected in 21 Escherichia coli strains recovered during 2003 from sick animals (11 of 459 [2.4%] strains) and healthy animals (10 of 158 [6.3%] strains) in Spain. Twelve of these strains harbored bla(CTX-M) genes and showed unrelated pulsed-field gel electrophoresis patterns… Leer más

Brinas L., Moreno MA., Teshager T., Saenz Y., Porrero MC., Dominguez L. y Torres C.

Paratuberculosis and avian tuberculosis infections in one red deer farm studied by IS900 and IS901 RFLP analysis

Veterinary Microbiology 105(3-4):261-8.

     As the attempt to eradicate paratuberculosis in one red deer (Cervus elaphus) farm failed, all 167 red deer of different age groups were slaughtered and examined by culture for mycobacteria, and the farm was closed down. Spleen and hepatic lymph nodes, mediastinal lymph node, ileocecal lymph node, and ileum were collected from each animal and examined (a total of 835 organs). Neither tuberculosis lesions nor pathognomic signs of paratuberculosis were detected. Among all microscopically negative for mycobacteria organs, Mycobacterium avium subsp. paratuberculosis alone was isolated from 165 organs, M. a. avium alone from 41 organs, and both pathogens from four organs. M. a. paratuberculosis alone was detected in 71 red deer, M. a. avium alon… Leer más

Machackova-Kopecna M., Bartos M., Straka M., Ludvik V., Svastova P., Alvarez J., Lamka J., Trcka I., Treml F., Parmova I. y Pavlik I.

Mycobacterium avium subspecies paratuberculosis in fallow deer and wild boar in Spain

The Veterinary Record 156(7):212-3.

     Mycobacterium avium subspecies paratuberculosis is the aetiological agent of a chronic granulomatous enteritis in ruminants known as Johne’s disease or paratuberculosis. It is considered to be one of the most serious diseases affecting dairy cattle worldwide, being responsible for significant economic losses (Harris and Barletta 2001). Its clinical relevance is commonly recognised in captive or farmed wild ruminants, but some studies have suggested that wild ruminant populations could also be an important natural reservoir of M avium subspecies paratuberculosis. The pathogen has already been isolated from a number of wild ruminant species, such as red deer (Cervus elaphus), fallow deer (Dama dama) and tule elk (Cervus elaphus nannodes) (Co… Leer más

Alvarez J., de Juan L., Briones V., Romero B., Aranaz A., Fernandez-Garayzabal JF. y Mateos A.

Antimicrobial susceptibility of clinical strains of Streptococcus suis isolated from pigs in Spain

Veterinary Microbiology 105(2):143-7.

     The antimicrobial susceptibility of 151 clinical Streptococcus suis strains isolated from diseased pigs in Spain was determined by a microdilution method. Isolates were mostly susceptible to beta-lactam antimicrobials, aminoglycosides, enrofloxacin, novobiocin and spectinomycin. More than 87% of the S. suis isolates were resistant to tetracyclines, sulphonamides, macrolides and clindamycin. Strains of serotype 9 were significantly more resistant than strains of serotype 2 (P<0.05) to tylosin (94% versus 77%) and clindamycin (94% versus 64%). Eighty-seven percent of the S. suis isolates were resistant to at least four antimicrobials and nine isolates (6%) were resistant to at least six antimicrobials. The most frequently identified multidrug… Leer más

Vela AI., Moreno MA., Cebolla JA., Gonzalez S., Latre MV., Dominguez L. y Fernandez-Garayzabal JF..

First identification of Streptococcus phocae isolated from Atlantic salmon (Salmo salar)

Journal Of Clinical Microbiology 43(1):526-7.

     Different genera and species of gram-positive, catalase-negative cocci are pathogenic to fish. The numbers of infections caused by these microorganisms have increased during the last decade, and such infections are responsible for significant economic losses in the fish farm industry.… Leer más

Gibello A., Mata AI., Blanco MM., Casamayor A., Dominguez L. y Fernandez-Garayzabal JF..