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Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus

Investigación publicada en The Veterinary quarterly

27 de marzo de 2024

Despite the great interest in the development of a vaccine against African swine fever (ASF) in wild boar, the immunological mechanisms that induce animal protection are still unknown. For this purpose, tertiary lymphoid organs (TLOs) of wild boar were characterised and compared with mucosa-associated lymphoid tissues (MALTs) by histopathology, histomorphometry and immunohistochemistry (CD3, CD79, PAX5, LYVE1, fibronectin). In addition, real-time polymerase chain reaction (qPCR) and immunohistochemistry (p72) were used to evaluate the presence of ASF virus (ASFV) in blood and tissues samples, respectively. TLOs were observed in animals infected with a low-virulent ASFV isolate (LVI), animals co-infected with low and high-virulent ASFV isolates (LVI-HVI) and animals infected only with the high virulence isolate (HVI). TLOs in LVI and LVI-HVI groups were located adjacent to the mucosa and presented a similar structure to MALT. Immunoexpresion of p72 observed in the inflammatory cells adjacent to TLOs/MALTs confirmed its development and reactivity generated by ASF attenuated isolates. Immunohistochemical evaluation, based on cellular composition (T and B lymphocytes), and histomorphometrical study revealed a more pronounced maturation of TLOs/MALTs in the LVI-HVI group. It is currently unclear whether these formations play a protective role by contributing to local immunity in chronic inflammatory diseases. However, the structural similarities between TLOs and MALTs and the location of TLOs close to the mucosa suggest that they may perform a similar function, facilitating a local protective response. Nevertheless, further investigations are warranted to assess the cellular and humoral dynamics of these lymphoid organs induced by attenuated isolates




Porras N., Sanchez-Vizcaino JM., Rodriguez-Bertos A., Kosowska A. y Barasona JA..




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Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus

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Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus



Participantes:

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Departamento de Medicina y Cirugía Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







The Veterinary quarterly
FACTOR YEAR Q
7.900 2023

NLMID: 7909485

PMID: 38533618

ISSN: 0165-2176



TÍTULO: Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus


REVISTA: Vet Q


NUMERACIÓN: 44(1):1-13


AÑO: 2024


EDITORIAL: Taylor & Francis


AUTORES: Porras N., Sanchez-Vizcaino JM., Rodriguez-Bertos A., Kosowska A. and Barasona JA..


First
Néstor Porras González
2nd
José Manuel Sánchez-Vizcaíno Rodríguez
3rd
Antonio Manuel Rodríguez Bertos
4th
Aleksandra Kosowska
Last
José Ángel Barasona García-Arévalo

DOI: https://doi.org/10.1080/01652176.2024.2331525


CITA ESTA PUBLICACIÓN:

Porras N., Sanchez-Vizcaino JM., Rodriguez-Bertos A., Kosowska A. y Barasona JA. Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus. The Veterinary quarterly. 44(1):1-13. 2024. (A). ISSN: 0165-2176. DOI: 10.1080/01652176.2024.2331525


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