Investigación

Sensibilidad antibiótica de Pasteurella multicocida de ovino

Trabajo Fin de Grado defendido por Mónica Sánchez Cauce

Caracterización y detección genética de Enterobacterias resistentes a colistina en muestras de origen animal

Marta Valero Lorenzo defendió este Trabajo Fin de Grado

Fundamento. La resistencia a colistina supone un grave problema de Salud Pública. La primera descripción de un gen plasmídico que codifica para resistencia a esta polimixina se realizó en 2015, habiéndose descrito hasta la fecha un total de ocho de estos genes. Generalmente, estos genes confieren resistencia mediante la modificación del lípido A, molécula diana de la colistina en la pared bacteriana, y, además, al estar codificados en plásmidos, pueden estar implicados en una transmisión horizontal de la resistencia. La reciente clasificación de la colistina por parte de la OMS como un “antibi… Leer más

Prevalencia de la Hepatitis E en cerdo blanco en España

Trabajo Fin de Grado defendido por Sara Carral Irijoa

El virus de la hepatitis E (HEV) es responsable de casos, endémicos y epidémicos, de hepatitis aguda en humanos en todo el mundo. En países en desarrollo la transmisión es fundamentalmente a partir del consumo de agua contaminada. En países desarrollados la hepatitis E se considera una enfermedad zoonótica emergente, siendo el consumo de alimentos contaminados, principalmente del cerdo, la principal fuente de infección. Es un virus ARN, clasificado en la familia Hepeviridae, que incluye 7 genotipos, 4 principales (1-4), y un octavo genotipo que está propuesto. Los genotipos 1 y 2 sólo afectan … Leer más

Pathologic findings and causes of death of stranded cetaceans in the Canary Islands (2006-2012)

PLoS ONE publica este artículo de investigación

This study describes the pathologic findings and most probable causes of death (CD) of 224 cetaceans stranded along the coastline of the Canary Islands (Spain) over a 7-year period, 2006-2012. Most probable CD, grouped as pathologic categories (PCs), was identified in 208/224 (92.8%) examined animals. Within natural PCs, those associated with good nutritional status represented 70/208 (33.6%), whereas, those associated with significant loss of nutritional status represented 49/208 (23.5%). Fatal intra- and interspecific traumatic interactions were 37/208 (17.8%). Vessel collisions included 24/… Leer más

Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level

Investigación publicada en Journal of Fish Diseases

The aim of this study was to evaluate the usefulness of the MALDI-TOF MS to identify 151 isolates of Aeromonas obtained mostly from diseased fish. MALDI-TOF MS correctly identified all isolates to the genus level but important differences in the percentage of isolates correctly identified depending on the species were observed. Considering exclusively the first identification option, Aeromonas bestiarum, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas veronii and Aeromonas sobria were the best identified with results >95%. However, considering the first and second identification options… Leer más

Management of hunting waste as control measure for tuberculosis in wild ungulates in south-central Spain

Transboundary and Emerging Diseases publica este artículo de investigación

In recent decades, habitat change and the intensive management of wild ungulates for hunting have led to an increase in their populations in south-central Spain. This implies a higher generation of hunting waste, which can favour the transmission of infectious diseases, including tuberculosis (TB). The aim of this study was to assess the usefulness of the proper disposal of hunting waste as TB control measure in wild boar (Sus scrofa) and red deer (Cervus elaphus) during the 2008/2009 to 2016/2017 hunting seasons. Blood samples from 664 wild boar and 934 red deer were obtained in 14 game estat… Leer más

Carriage of antibiotic-resistant bacteria in urban versus rural wild boars

Artículo de investigación publicado en European Journal of Wildlife Research

The Western European population of wild boar (Sus scrofa) has increased its distribution over the past several decades, and some populations have colonized areas strongly influenced by human activity. Wild boars are known carriers of antibiotic-resistant bacteria acquired from the environment, and urban populations of wild boars may be more exposed than their rural counterparts. In this work, we compared the frequency of antibiotic resistance in indicator bacteria (Escherichia coli, Enterococcus faecalis, Enterococcus faecium) isolated from urban wild boars with that from rural wild boars in N… Leer más

Evaluation of the performance of the IDvet IFN-gamma test for diagnosis of bovine tuberculosis in Spain

Artículo de investigación publicado en Frontiers in veterinary science

In Spain, the national bovine tuberculosis (bTB) eradication program is based on yearly skin testing of every ≥6 weeks old animal using the single or comparative tuberculin test and parallel use of the interferon-gamma (IFN-γ) assay as an ancillary diagnostic test in infected herds. There are several versions of the latter. Recently, a new commercial IDvet IFN-γ assay has been authorized for use in the program, but there is limited scientific evidence about its performance in different epidemiological settings. Therefore, two studies to evaluate the performance of the IDvet ass… Leer más

Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguays University Hospital Identifies the First rmtC Gene in the Species

Microbial Drug Resistance (Larchmont, N.Y.) publica este artículo de investigación

Carbapenem-resistant Acinetobacter baumannii (CRAB) infections are an increasing concern in intensive care units (ICUs) worldwide. The combination of carbapenemases and 16S rRNA-methyltransferases (16S-RMTases) further reduces the therapeutic options. OXA-carbapenemase/A. baumannii clone tandems in Latin America have already been described; however, no information exists in this region regarding the occurrence of 16S-RMTases in this microorganism. In addition, the epidemiology of A. baumannii in ICUs and its associated resistance profiles are poorly understood. Our objectives were as follows: … Leer más

Novel and highly sensitive sybr® green real-time pcr for poxvirus detection in odontocete cetaceans

Investigación publicada en Journal of Virological Methods

Poxviruses are emerging pathogens in cetaceans, temporarily named `Cetaceanpoxvirus` (CePV, family Poxviridae), classified into two main lineages: CePV-1 in odontocetes and CePV-2 in mysticetes. Only a few studies performed the molecular detection of CePVs, based on DNA-polymerase gene and/or DNA-topoisomerase I gene amplification. Herein we describe a new real-time PCR assay based on SYBR® Green and a new primer set to detect a 150 bp fragment of CePV DNA-polymerase gene, also effective for conventional PCR detection. The novel real-time PCR was able to detect 5 up to 5 × 106 copi… Leer más