Investigación

Effect of heat-inactivated Mycobacterium avium subspecies paratuberculosis (MAP) vaccine on the lesions and immunopathology developed in target tissues of naturally MAP-infected goats

Investigación publicada en Veterinary Microbiology

Paratuberculosis (PTB) is a disease caused by Mycobacterium avium subspecies paratuberculosis (MAP), which affects a broad range of hosts, including domestic and wild animals. PTB is a chronic granulomatous enteritis and lymphadenitis that compromises animal welfare and causes economic losses. The objective of this study was to evaluate the effect of a commercial heat-inactivated MAP vaccine on lesions and immunopathology developed in the target tissues of goats naturally infected with MAP. Lesions compatible with PTB in the intestine and regional lymph nodes (LNs), as well as local immune res… Leer más

Modelo experimental para Streptococcus suis: evaluación comparativa de dos técnicas dirigidas a la determinación de la presencia del patógeno en tejidos

Trabajo Fin de Grado defendido por Andrea Brigidano Lopez

Streptococcus suis (S. suis) es uno de los patógenos más relevantes en sanidad animal a los que se enfrenta actualmente el sector porcino a nivel mundial. Además, tiene un carácter zoonótico de importancia para la salud pública. Sin embargo, las estrategias disponibles para su control son limitadas, especialmente en lo referido a la disponibilidad de vacunas comerciales, debido a la ausencia de inmunidad cruzada asociado a su gran variabilidad antigénica. Es por ello por lo que la utilización de modelos animales para el estudio de candidatos vacunales y el empleo de métodos diagnósticos sensib… Leer más

Evaluación de la serología para el diagnóstico de la tuberculosis en cérvidos

Eva Barrio Calvo defendió este Trabajo Fin de Grado

Fundamento y objetivo: Los cérvidos son reservorios de la tuberculosis (TB) para el ganado doméstico. La vigilancia de TB en cérvidos se basa sobre todo en el examen de lesiones compatibles con tuberculosis (LCT), cultivo e intradermotuberculinización (IDTB), aunque estas pruebas tienen ciertas limitaciones. Además, el Reglamento Delegado (UE) 2020/688 no especifica la prueba diagnóstica requerida para el movimiento de cérvidos dentro de la Unión Europea. Para este fin, es necesario una prueba rápida y coste-efectiva, entre las que se encuentran las pruebas de detección de anticuerpos. El obje… Leer más

Patterns of antimicrobial resistance in Salmonella isolates from fattening pigs in Spain

Artículo de investigación publicado en BMC Veterinary Research

Swine are considered a major source of foodborne salmonellosis, a public health issue further complicated by the circulation of multidrug-resistant Salmonella strains that threaten the safety of the food chain. The current study aimed to identify patterns that can help to understand the epidemiology of antimicrobial resistance (AMR) in Salmonella in pigs in Spain through the application of several multivariate statistical methods to data from the AMR national surveillance programs from 2001 to 2017. A total of 1,318 pig Salmonella isolates belonging to 63 different serotypes were isolated a… Leer más

- El antibiótico frente al que presenta más resistencia la salmonela en cerdos de España - animalshealth.es

Evaluation of the clinical evolution and transmission of SARS-CoV-2 infection in cats by simulating natural routes of infection

Investigación publicada en Veterinary Research Communications

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the current pandemic disease denominated as Coronavirus Disease 2019 (COVID-19). Several studies suggest that the original source of this virus was a spillover from an animal reservoir and its subsequent adaptation to humans. Of all the different animals affected, cats are one of the most susceptible species. Moreover, several cases of natural infection in domestic and stray cats have been reported in the last few months. Although experimental infection assays have demonstrated that cats are successfully inf… Leer más

Harmonisation of in-silico next-generation sequencing based methods for diagnostics and surveillance

Scientific reports publica este artículo de investigación

Improvements in cost and speed of next generation sequencing (NGS) have provided a new pathway for delivering disease diagnosis, molecular typing, and detection of antimicrobial resistance (AMR). Numerous published methods and protocols exist, but a lack of harmonisation has hampered meaningful comparisons between results produced by different methods/protocols vital for global genomic diagnostics and surveillance. As an exemplar, this study evaluated the sensitivity and specificity of five well-established in-silico AMR detection software where the genotype results produced from running a pan… Leer más

Hidden dissemination of carbapenem-susceptible OXA-48-producing Proteus mirabilis

Investigación publicada en The Journal of Antimicrobial Chemotherapy

Objectives: To detect a potential hidden dissemination of the blaOXA-48 gene among Proteus mirabilis isolates obtained from a single centre. Methods: P. mirabilis from diverse clinical samples presenting an ESBL phenotype or obtained from blood cultured from 2017 to 2019 were evaluated. Bacterial identification was performed using MALDI-TOF MS. MICs were determined using International Organization for Standardization (ISO) standard microdilution and interpreted following EUCAST guidelines. WGS was performed using both short- and long-read technologies and assemblies were done using Unicycler.… Leer más

The Omicron (B.1.1.529) SARS-CoV-2 variant of concern also affects companion animals

Artículo de investigación publicado en Frontiers in veterinary science

The emergence of the Omicron variant (B.1. 1.529) has brought with it an increase in the incidence of SARS-CoV-2 disease. However, there is hardly any data on its incidence in companion animals. We have detected the presence of this new variant in domestic animals (dogs and cats) living with infected owners in Spain. None of the RT-qPCR positive animals (10.13%) presented any clinical signs and the viral loads detected were low. In addition, the shedding of viral RNA lasted a short period of time in the positive animals. Infection with this variant of concern (VOC) was confirmed by RT-qPCR and… Leer más

The expression of aminoglycoside resistance genes in integron cassettes is not controlled by riboswitches

Nucleic Acids Research publica este artículo de investigación

Regulation of gene expression is a key factor influencing the success of antimicrobial resistance determinants. A variety of determinants conferring resistance against aminoglycosides (Ag) are commonly found in clinically relevant bacteria, but whether their expression is regulated or not is controversial. The expression of several Ag resistance genes has been reported to be controlled by a riboswitch mechanism encoded in a conserved sequence. Yet this sequence corresponds to the integration site of an integron, a genetic platform that recruits genes of different functions, making the presence… Leer más

- Desmienten una teoría sobre resistencia a antibióticos nueve años después - ucm.es

- Un equipo de científicos españoles desmiente una teoría de resistencia a antibióticos publicada hace nueve años - phmk.es

- Investigadores españoles desmienten una teoría sobre resistencia a antibióticos - eldebate.com

- Nuevas aportaciones nacionales al estudio de la resistencia a los antibióticos - imfarmacias.es

- Veterinarios españoles desmienten una teoría sobre resistencia a antibióticos 9 años después - animalshealth.es

Budd-Chiari-like pathology in dolphins

Scientific reports publica este artículo de investigación

Nearly two decades ago, pathologic examination results suggested that acoustic factors, such as mid-frequency active naval military sonar (MFAS) could be the cause of acute decompression-like sickness in stranded beaked whales. Acute systemic gas embolism in these whales was reported together with enigmatic cystic liver lesions (CLL), characterized by intrahepatic encapsulated gas-filled cysts, tentatively interpreted as "gas-bubble" lesions in various other cetacean species. Here we provide a pathologic reinterpretation of CLL in odontocetes. Among 1,200 cetaceans necropsied, CLL were only ob… Leer más