Investigación

Disease-mediated piglet mortality prevents wild boar population growth in fenced overabundant settings

Investigación publicada en European Journal of Wildlife Research

Assessing Eurasian wild boar (Sus scrofa) mortality is a key for understanding population dynamics and adjusting hunting harvest and population management. We used radio-tagging and video-trapping to quantify piglet summer mortality in a managed (i.e. fenced and year-round fed) wild boar population from southern Spain. We used two independent tools to assess wild boar mortality, i.e. radio-tracking and video footage. A total of 32 wild boar piglets were captured at selective feeders acting as cage-traps, and all the piglets were equipped with ear-tag transmitters including a mortality sensor. … Leer más

Dynamic Network of Interactions in the Wildlife-Livestock Interface in Mediterranean Spain: An Epidemiological Point of View

Pathogens publica este artículo de investigación

The correct management of diseases that are transmitted between wildlife and livestock requires a reliable estimate of the pathogen transmission rate. The calculation of this parameter is a challenge for epidemiologists, since transmission can occur through multiple pathways. The social network analysis is a widely used tool in epidemiology due to its capacity to identify individuals and communities with relevant roles for pathogen transmission. In the present work, we studied the dynamic network of interactions in a complex epidemiological scenario using information from different methodologi… Leer más

A ten-year-surveillance program of zoonotic pathogens in feral pigeons in the City of Madrid (2005-2014): The importance of a systematic pest control

Artículo de investigación publicado en Research in Veterinary Science

Feral pigeons have increased in urban settings worldwide becoming a potential health risk for humans and other animals. Control and surveillance programs are essential to prevent the possible transmission of zoonotic pathogens carried by pigeons. A surveillance program was carried out in Madrid City (Spain) during 2005–2014 to determine the role of urban pigeons as carriers of zoonotic agents comparing these results with studies performed elsewhere in the last fifteen years. A total of 1372 pigeons were randomly captured and tested for detection of Antimicrobial susceptibility and genetic h… Leer más

Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk

Investigación publicada en Research in Veterinary Science

Caprine tuberculosis (TB) is a zoonosis with sanitary and economic repercussions. Caprine TB control programs are based on a test and cull strategy using the intradermal tuberculin tests and slaughterhouse surveillance. However, this approach is not always feasible and may have a limited sensitivity under specific circumstances. In this study, performance of a new experimental test based on the P22 protein complex (P22 ELISA) was evaluated in two TB-infected herds using milk and serum samples and compared with cell-based diagnostic tests. Samples from a low (n = 62, herd 1) and a high (n = 52,… Leer más

Comparison of the sensitivity of laryngeal swabs and deep tracheal catheters for detection of Mycoplasma hyopneumoniae in experimentally and naturally infected pigs early and late after infection

Investigación publicada en Veterinary Microbiology

Detection of Mycoplasma hyopneumoniae infection in live pigs is a critical component to measure the success of disease control or elimination strategies. However, in vivo diagnosis of M. hyopneumoniae is difficult and the imperfect sensitivity of diagnostic tools has been deemed as one of the main challenges. Here, the sensitivity of laryngeal swabs and deep tracheal catheters for detection of M. hyopneumoniae early and late after infection was determined using inoculation status as a gold standard in experimentally infected pigs and a Bayesian approach in naturally infected pigs. Three-hundre… Leer más

- Detección de Mycoplasma hyopneumoniae – ¿Hisopado de laringe o catéter traqueal? - porcino.info

Prevalence and time trend analysis of antimicrobial resistance in respiratory bacterial pathogens collected from diseased pigs in USA between 2006-2016

Artículo de investigación publicado en Research in Veterinary Science

Swine respiratory disease complex (SRDC) causes massive economic losses to the swine industry and is a major animal welfare concern. Antimicrobials are mainstay in treatment and control of SRDC. However, there is a lack of data on the prevalence and trends in resistance to antimicrobials in bacterial pathogens associated with SRDC. The objective of this study was to estimate the prevalence and changes in resistance to 13 antimicrobials in swine bacterial pathogens (Streptococcus suis, Pasteurella multocida, Actinobacillus suis and Haemophilus parasuis) in the U.S.A using data collected at Univ… Leer más

Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017

Investigación publicada en Frontiers in Microbiology

Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver-derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from 9 Spanish slaughterhouses (50% National production), that could enter into the food supply chain. Anti-HEV antibod… Leer más

Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration

Cell metabolism publica este artículo de investigación

Life on Earth emerged in a hydrogen sulfide (H2S)-rich environment eons ago and with it protein persulfidation mediated by H2S evolved as a signaling mechanism. Protein persulfidation (S-sulfhydration) is a post-translational modification of reactive cysteine residues, which modulate protein structure and/or function. Persulfides are difficult to label and study due to their reactivity and similarity with cysteine. Here, we report a facile strategy for chemoselective persulfide bioconjugation using dimedone-based probes, to achieve highly selective, rapid, and robust persulfide labeling in bio… Leer más

Econometric ARIMA methodology to elucidate the evolution of trends in nosocomial antimicrobial resistance rates in the European Union

International Journal of Antimicrobial Agents publica este artículo de investigación

BACKGROUND: Infectious pathogens harboring cephalosporin or fluoroquinolones resistance constitute a serious hazard to human health. OBJECTIVES: The aim of this study is to set up a methodology based on econometric analysis and largest European Union (EU) resistance database (EARS-Net) to model EU nosocomial antimicrobial resistance (AMR) and to detect tendency changes, steps or peaks. The contribution of legislation based on third generation cephalosporin (3GC) and fluoroquinolones (FQ) class referrals on EU resistance rate patterns is evaluated. METHODS: We studied 3GC and FQ resistance… Leer más

Identifying emerging trends in antimicrobial resistance using Salmonella surveillance data in poultry in Spain

Artículo de investigación publicado en Transboundary and Emerging Diseases

Despite of controls and preventive measures implemented along the food chain, infection with non-typhoidal Salmonella (NTS) remains one of the major causes of foodborne disease worldwide. Poultry is considered one of the major sources of NTS. This has led to the implementation of monitoring and control programs in many countries (including Spain) to ensure that in poultry flocks infection is kept to a minimum and to allow the identification and monitoring of circulating NTS strains and their antimicrobial resistance (AMR) phenotypes. Here, we investigated the information from the monitoring pr… Leer más