Investigación

Evolution of ColE1-like plasmids across γ-Proteobacteria: From bacteriocin production to antimicrobial resistance

Investigación publicada en PLoS Genetics

Antimicrobial resistance is one of the major threats to Public Health worldwide. Understanding the transfer and maintenance of antimicrobial resistance genes mediated by mobile genetic elements is thus urgent. In this work, we focus on the ColE1-like plasmid family, whose distinctive replication and multicopy nature has given rise to key discoveries and tools in molecular biology. Despite being massively used, the hosts, functions, and evolutionary history of these plasmids remain poorly known. Here, we built specific Hidden Markov Model(HMM) profiles to search ColE1 replicons within genomes. … Leer más

Differences in wild boar spatial behaviour among land uses and management scenarios in Mediterranean ecosystems

Investigación publicada en Science of the total environment

The ubiquitous wild boar is causing diverse and growing conflicts of socio-ecological and economic relevance worldwide. For that reason, knowledge of its spatial ecology is crucial to designing effective management programmes. But this knowledge is scarce in Mediterranean areas with mixed land uses. We describe the spatial ecology and habitat selection of 41 adult wild boar monitored using GPS collars and analyse the effects of sex and the period (food shortage period, hunting season and food abundance period) under different land uses (protected areas, mixed farms and fenced hunting estates).… Leer más

Avances en el conocimiento de la fisiopatología del buceo en tortugas marinas: El descubrimiento de la enfermedad descompresiva

Daniel García Párraga defendió su tesis doctoral en la Facultad de Veterinaria de la Universidad Complutense de Madrid

La enfermedad descompresiva (DCS), diagnosticada clínicamente mediante la reversión de los síntomas tras el tratamiento de recompresión, no había sido nunca antes reportada en vertebrados buceadores en apnea, a pesar de la previsible aparición en sus tejidos de tensiones de gas suficientemente elevadas como para dar lugar a la formación de burbujas y lesiones en base a los estudios en modelos experimentales terrestres. En mamíferos marinos, han sido descritas lesiones compatibles con DCS en estudios post mortem, en casos en los que se presupone una alteración del comportamiento de buceo como c… Leer más

Bottlenose dolphins (Tursiops truncatus) aggressive behavior towards other cetacean species in the western Mediterranean

Scientific reports publica este artículo de investigación

Aggressive behavior of bottlenose dolphins (Tursiops truncatus) towards conspecifics is widely described, but they have also often been reported attacking and killing harbour porpoises (Phocoena phocoena) around the world. However, very few reports exist of aggressive interactions between bottlenose dolphins and other cetacean species. Here, we provide the first evidence that bottlenose dolphins in the western Mediterranean exhibit aggressive behavior towards both striped dolphins (Stenella coeruleoalba) and Risso`s dolphins (Grampus griseus). Necropsies and visual examination of stranded stri… Leer más

A new method for sampling African swine fever virus genome and its inactivation in environmental samples

Investigación publicada en Scientific reports

African swine fever (ASF) is currently the most dangerous disease for the global pig industry, causing huge economic losses, due to the lack of effective vaccine or treatment. Only the early detection of ASF virus (ASFV) and proper biosecurity measures are effective to reduce the viral expansion. One of the most widely recognized risks as regards the introduction ASFV into a country is infected animals and contaminated livestock vehicles. In order to improve ASF surveillance, we have assessed the capacity for the detection and inactivation of ASFV genome by using Dry-Sponges (3 M) pre-hydrated… Leer más

Large-scale study on virological and serological prevalence of SARS-CoV-2 in cats and dogs in Spain

Artículo de investigación publicado en Transboundary and Emerging Diseases

The disease produced by the severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), is currently one of the primary concerns worldwide. Knowing the zoonotic origin of the disease and that several animal species, including dogs and cats, are susceptible to viral infection, it is critical to assess the relevance of pets in this pandemic. Here, we performed a large-scale study on SARS-CoV-2 serological and viral prevalence in cats and dogs in Spain in order to elucidate their role and susceptibility. Samples from animals in contact with COVID-19 positive people and/or compatible sym… Leer más

- Estudian la prevalencia serológica de SARS-CoV-2 en mascotas españolas - diarioveterinario.com

Equine infection with Leishmania spp. in Costa Rica: Study of five cases

Investigación publicada en Veterinary Medicine and Science

Cutaneous forms of leishmaniosis due to Leishmania braziliensis have been reported in horses in the New World. Domestic animals play a role in the transmission of the disease. In Costa Rica, human cases of L. braziliensis, L. panamensis and L. infantum have been reported. The present report describes five cases of equine cutaneous leishmaniosis in Costa Rica. The aetiological diagnosis was based on the presence of the parasite within the lesions. Skin biopsies were used to perform histopathological analyses of the lesions. Immunohistochemistry was used to detect the presence of the Leishmania … Leer más

- Veterinarios españoles describen 5 casos de leishmaniosis equina - animalshealth.es

Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions

Artículo de investigación publicado en Viruses-Basel

The monitoring of herpesvirus infection provides useful information when assessing marine mammals’ health. This paper shows the prevalence of herpesvirus infection (80.85%) in 47 cetaceans stranded on the coast of the Valencian Community, Spain. Of the 966 tissues evaluated, 121 tested positive when employing nested-PCR (12.53%). The largest proportion of herpesvirus-positive tissue samples was in the reproductive system, nervous system, and tegument. Herpesvirus was more prevalent in females, juveniles, and calves. More than half the DNA PCR positive tissues contained herpesvirus RNA, indicat… Leer más

Susceptibility testing of Prototheca bovis isolates from cases of bovine mastitis using the CLSI reference broth microdilution method and the Sensititre YeastOne colorimetric panel

Artículo de investigación publicado en Medical Mycology

A total of 62 Prototheca bovis isolates from cases of bovine mastitis were tested for susceptibility to different antifungal compounds by the Clinical and Laboratory Standards Institute (CLSI) reference microdilution method and a commercial colorimetric microdilution panel (Sensititre YeastOne). All isolates displayed low susceptibility to echinocandins (MICs > 8 μg/ml for anidulafungin, caspofungin, and micafungin), flucytosine (MIC > 64 μg/ml), and the azoles enilconazole and fluconazole (MICs > 4 and > 64 μg/ml, respectively). Moreover, 45.2%, 32.3%, and 1.6% of isolates had … Leer más

Genomic and Pathogenic Investigations of Streptococcus suis Serotype 7 Population derived from a Human Patient and Pigs

Investigación publicada en Emerging microbes and infections

Streptococcus suis is one of the important emerging zoonotic pathogens. Serotype 2 is most prevalent in patients worldwide. In the present study, we first isolated one S. suis serotype 7 strain GX69 from the blood culture of a patient with septicemia complicated with pneumonia in China. In order to deepen the understanding of S. suis serotype 7 population characteristics, we investigated the phylogenetic structure, genomic features, and virulence of S. suis serotype 7 population, including 35 strains and 79 genomes. Significant diversities were revealed in S. suis serotype 7 population, which … Leer más