Investigación

The plasmid-mediated Kluyvera-like arnBCADTEF operon confers colistin (hetero) resistance to Escherichia coli

Antimicrobial Agents and Chemotherapy publica este artículo de investigación

The use of colistin as a last resort antimicrobial is compromised by the emergence of resistant enterobacteria with acquired determinants like mcr genes, mutations that activate the PmrAB system and by still unknown mechanisms. This work analyzed 74 E. coli isolates from healthy swine, turkey or bovine, characterizing their colistin resistance determinants. The mcr-1 gene, detected in 69 isolates, was the main determinant found among which 45% were carried by highly mobile plasmids, followed by four strains lacking previously known resistance determinants or two with mcr-4 (one in addition to … Leer más

Population genomics and antimicrobial resistance dynamics of Escherichia coli in wastewater and river environments

Investigación publicada en Communications Biology

Aquatic environments are key niches for the emergence, evolution and dissemination of antimicrobial resistance. However, the population diversity and the genetic elements that drive the dynamics of resistant bacteria in different aquatic environments are still largely unknown. The aim of this study was to understand the population genomics and evolutionary events of Escherichia coli resistant to clinically important antibiotics including aminoglycosides, in anthropogenic and natural water ecosystems. Here we show that less different E. coli sequence types (STs) are identified in wastewater tha… Leer más

Effect of Biscuit Flour and Fermented Defatted Alperujo Co-Administration on Intestinal Mucosa Morphology and Productive Performance in Laying Hens

Artículo de investigación publicado en Animals

In this study, the effects of co‐administration with biscuit flour and fermented defatted “alperujo” (FDA) on gut health were evaluated in a batch of laying hens (Hy‐Line 2015) on a commercial farm. Animals were divided into two groups: control group and treatment group; and histological and morphometric analyses of all sections of the intestine (duodenum, jejunum, ileum, cecum and rectum) were performed at 10, 18, 25, 50 and 75 weeks of age. During the whole productive period, a decrease in the mortality rate (p = 0.01) was observed in treated hens, as well as an increase in the n… Leer más

Spoligotype-specific risk of finding lesions in tissues from cattle infected by Mycobacterium bovis

BMC Veterinary Research publica este artículo de investigación

Although the pathogenic effect of members of the Mycobacterium tuberculosis complex in susceptible hosts is well known, differences in clinical signs and pathological findings observed in infected animals have been reported, likely due to a combination of host and pathogen-related factors. Here, we investigated whether Mycobacterium bovis strains belonging to different spoligotypes were associated with a higher risk of occurrence of visible/more severe lesions in target organs (lungs and/or lymph nodes) from infected animals. A large collection of 8889 samples belonging to cattle were classifi… Leer más

Polyresistant Mycobacterium bovis Infection in Human and Sympatric Sheep, Spain, 2017-2018

Artículo de investigación publicado en Emerging Infectious Diseases

The main etiologic agent of tuberculosis (TB) in livestock is Mycobacterium bovis; human TB cases caused by M. bovis are rare. Analysis of a TB outbreak caused by polyresistant M. bovis involving a human and sympatric sheep in Spain suggests local circulation of drug-resistant M. bovis strains among livestock… Leer más

- Caso zoonótico de tuberculosis resistente a antibióticos en España - diarioveterinario.com

Evaluation of the performance of slaughterhouse surveillance for bovine tuberculosis detection in Castilla y Leon, Spain

Artículo de investigación publicado en Preventive Veterinary Medicine

Post-mortem inspection (PMI) of routinely slaughtered cattle in abattoirs is an extremely valuable tool for detecting bovine tuberculosis (bTB) infected herds that can supplement active surveillance activities. However, its true performance is difficult to assess due to the multiple factors that may affect it. Here, we determined relative efficiencies in the detection of bTB-compatible lesions and probabilities of subsequent laboratory confirmation of abattoirs located in Castilla y Leon, one of the regions with the largest cattle population in Spain, between 2010 and 2017. The slaughtered ani… Leer más

Antibiotic use in the COVID-19 crisis in Spain

Investigación publicada en Clinical Microbiology and Infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

Before the coronavirus disease 2019 (COVID-19) pandemic, antimicrobial resistance was the world`s most pressing health crisis. The United Nations, the World Health Organization (WHO) and the European Center for Disease Control and Prevention have made antimicrobial resistance a top priority, establishing a task force in the G-20 and organizing a joint program initiative on antimicrobial resistance including countries from al over the world [1]. However, with the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus pandemic in January 2020, all efforts are now foc… Leer más

Towards a Better and Harmonized Education in Antimicrobial Stewardship in European Veterinary Curricula

Investigación publicada en Antibiotics

Education in antimicrobial stewardship (AMS) in veterinary medicine is essential to foster responsible antimicrobial use and control of antimicrobial resistance (AMR) in animals. AMS is listed by the EU and international organizations among the basic `Day One Competences` required of veterinary students upon graduation. Our aim was to evaluate the quality of education of European veterinary students in AMS. We distributed a 27-item survey addressing the perceptions of preparedness and acquired skills on key topics related to AMS to final-year veterinary students in Europe. We collected 3423 co… Leer más

Transmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmer

Antibiotics publica este artículo de investigación

Colistin use has mostly been stopped in human medicine, due to its toxicity. However, nowadays, it still is used as a last-resort antibiotic to treat hospital infections caused by multi-drug resistant Enterobacteriaceae. On the contrary, colistin has been used in veterinary medicine until recently. In this study, 210 fecal samples from pigs (n = 57), calves (n = 152), and the farmer (n = 1) were collected from a farm where E. coli harboring mcr-1–mcr-3 was previously detected. Samples were plated, and mcr-genes presence was confirmed by multiplex-PCR. Hybrid sequencing which determined the pre… Leer más

High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge

Artículo de investigación publicado en Vaccines

Abstract: African swine fever (ASF) is currently the major concern of the global swine industry, as a consequence of which a reconsideration of the containment and prevention measures taken to date is urgently required. A great interest in developing an effective and safe vaccine against ASF virus (ASFV) infection has, therefore, recently appeared. The objective of the present study is to test an inactivated ASFV preparation under a vaccination strategy that has not previously been tested in order to improve its protective effect. The following have been considered: (i) virus inactivation by … Leer más