Investigación

First report and molecular characterization of cases of natural Taylorella asinigenitalis infection in three donkey breeds in Spain

Investigación publicada en Veterinary Microbiology

Taylorella asinigenitalis is a non-pathogenic bacteria isolated from the genital tract of donkeys but also a cause of metritis and vaginal discharge in mares. It is closely related to Taylorella equigenitalis, the cause of Contagious Equine Metritis (CEM) in horses, and has been present in different countries in Europe since 1995. Up to date, there are no studies on the prevalence of T. asinigenitalis in the equine or asinine populations in Spain; this is the first report of the presence of T. asinigenitalis in donkeys (Equus asinus) from different breeds in three regions of Spain. A total of … Leer más

Exploiting 16S rRNA-based metagenomics to reveal neglected microorganisms associated with infertility in breeding bulls in Spanish extensive herds

Research in Veterinary Science publica este artículo de investigación

Bovine infectious infertility represents a problem due to the high impact on animal production and, in many cases, in public health. A lack of information on the characteristics of the bacterial population of the bovine reproductive system can hamper a comprehensive understanding of reproductive pathologies and the role that the microbiome could play. A metagenomic study based on the V3-V4 hypervariable region of the bacterial 16S rRNA gene was performed in 1029 preputial samples from bulls raised in an extensive regimen in Spain (944 from herds with low fertility rates -case group-, and 85 sa… Leer más

Identifying high-risk areas for dog-mediated rabies using Bayesian spatial regression

Artículo de investigación publicado en One Health

Despite ongoing control efforts, rabies remains an endemic zoonotic disease in many countries. Determining high-risk areas and the space-time patterns of rabies spread, as it relates to epidemiologically important factors, can support policymakers and program managers alike to develop evidence-based targeted surveillance and control programs. In this One Health approach which selected Thailand as the example site, the location-based risk of contracting dog-mediated rabies by both human and animal populations was quantified using a Bayesian spatial regression model. Specifically, a conditional … Leer más

Animal abuse in Spanish-breed fighting cocks (Gallus gallus domesticus)

Artículo de investigación publicado en Forensic Science International

Cockfighting is illegal both in the European Union and the United States, although fighting cock husbandry and breed preservation are allowed. Here, we describe the pathological findings observed in the carcasses of two Spanish-breed fighting cocks submitted to our laboratory after an animal abuse-suspected death. The postmortem examination revealed these cocks may have been used for cockfighting (comb, ear lobes, wattles, and chest feather removal; and trimmed spurs). The severe extensive hemorrhages observed in the cervical region of both birds, affecting C1-C4 in bird No. 1 and C8-C11 in No… Leer más

Determinación de intervalos de referencia de parámetros hematológicos, bioquímicos y de estrés oxidativo en caballos residentes en centros hípicos en la zona central de España

Tesis Doctoral defendida por Aránzazu Buendía Andrés en la Facultad de Veterinaria de la Universidad Complutense de Madrid

En la actualidad, una de las principares áreas del sector equino en España es la del uso del caballo para actividades de ocio y competición en centros hípicos. El veterinario clínico de équidos desempeña un papel fundamental en la vigilancia de la salud de estos caballos, y una de las herramientas más frecuentemente utilizadas es la realización de análisis clinicos. Para la correcta interpretación de estos análisis son necesarios los intervalos de referencia, definidos como el rango de valores de una variable en una población con un estado de salud definido. Hasta la fecha, la población eq… Leer más

Intestinal T-cell lymphoma in an Asian small-clawed otter: case report and literature review of lymphoma in the subfamily Lutrinae

Artículo de investigación publicado en Journal of Veterinary Diagnostic Investigation

The Asian small-clawed otter (Aonyx cinereus) is an endangered species that is common in zoologic collections. A 17-y-old female Asian small-clawed otter under human care, with a clinical history of chronic renal disease, was euthanized because of deteriorating health. Histologically, the jejunal wall was infiltrated by a monomorphic population of small neoplastic lymphocytes that expanded the lamina propria of the villi and crypts, and on rare occasions invaded the submucosa. The tumor was composed of T cells (CD3+) with a proliferation index of 16%. Based on the World Health Organization (WH… Leer más

Effect of a recent parenteral dexamethasone and ketoprofen administration on the immunological diagnosis of tuberculosis in goats

Investigación publicada en Frontiers in veterinary science

Caprine tuberculosis (TB) is a zoonosis caused by members of the Mycobacterium tuberculosis complex (MTBC). Caprine TB eradication programmes are based mainly on intradermal tuberculin tests and slaughterhouse surveillance. Different factors may affect the performance of the TB diagnostic tests used in caprine herds and, therefore, their ability to detect infected animals. The present study evaluates the effect of the fraudulent administration of two anti-inflammatory substances, dexamethasone and ketoprofen, on the performance of the TB diagnostic techniques used in goats, as well as the suit… Leer más

P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection

Comparative Immunology, Microbiology and Infectious Diseases publica este artículo de investigación

The P22 ELISA was recently developed for the serodiagnosis of animal tuberculosis. Herein, the stability of the P22 antigen in different presentations and storage conditions, and the cross-reactivity with Corynebacterium pseudotuberculosis infection in small ruminants were evaluated. For the stability assay, serum samples from cows, sheep, goats, alpacas, badgers, and wild boar were used in the P22 ELISA. The cross-reactivity analysis used sera from sheep and goats with caseous lymphadenitis (CLA). Differences in the immune recognition of P22 were found when the antigen was stored at 40 °C, bu… Leer más

A subunit vaccine candidate based on the Spike protein of SARS-CoV-2 prevents infectious virus shedding in cats

Investigación publicada en Research in Veterinary Science

Of the numerous animal species affected by the SARS-CoV-2 virus, cats are one of the most susceptible, and cat-to-cat transmission has been described. Although cat-to-human infection has not, as yet, been demonstrated, preventive measures should be taken in order to avoid both viral infection in cats and transmission among them. In this respect, the application of an effective vaccine to at-risk populations would be a useful tool for controlling the disease in this species. Here, we test a new vaccine prototype based on the Spike protein of the virus in order to prevent infection and infectiou… Leer más

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Increasing the data on elasmobranch plasma protein electrophoresis: electrophoretogram reference values determination in the undulate skate (Raja Undulata) and the nursehound shark (Scyliorhinus stellaris) maintained under human care

Artículo de investigación publicado en BMC Veterinary Research

Background: This study determined plasma protein electrophoresis (PPE) reference intervals in two elasmobranch species: the undulate skate (Raja undulata) and the nursehound shark (Scyliorhinus stellaris), using a reference population of 48 undulate skates (27 males, 21 females) and 62 nursehounds (32 males, 30 females), considered to be clinically healthy. Plasma samples were analyzed using capillary zone electrophoresis (CZE). Results: The undulate skate electrophoretogram resembled those previously reported in other batoids and could be divided into seven consistent fractions. No statistic… Leer más