Investigación

The bla NDM-1 carrying IncA/C2 plasmid underlies structural alterations and co-integrate formation in vivo

Antimicrobial Agents and Chemotherapy publica este artículo de investigación

In 2012, a carbapenemase-producing Salmonella (S.) Corvallis carrying a bla NDM-1 multi-resistance IncA/C2 plasmid, apart from IncHI2 and ColE-like plasmids, was detected in a wild bird in Germany. In recent broiler chicken infection study, we observed transfer of this bla NDM-1-carrying IncA/C2 plasmid to other Enterobacteriaceae Here, we focused on the stability of this plasmid and gained insight into type and frequency of its structural alterations after an in vivo passage in a broiler infection study… Leer más

Identificación de factores predictores de virulencia en cepas de Mycobacterium bovis

Alberto Gómez Buendía defendió este Trabajo Fin de Grado

Detección molecular de Trypanosoma cruzi en pelo de ratón infectado experimentalmente

Trabajo Fin de Grado defendido por Silvia Díaz Franchy

Trypanosoma cruzi es el parásito protozoo causante de la enfermedad de Chagas, que afecta entre 6 y 8 millones de personas. Es una enfermedad transmitida principalmente por insectos de la familia Reduviidae, aunque también se puede transmitir por vía oral, mediante transfusiones o vía congénita. Se trata de una enfermedad infradiagnosticada en Europa, siendo España el país europeo con mayor número de personas afectadas por T. cruzi. En los países endémicos los animales salvajes suponen uno de los principales reservorios, haciendo el manejo y obtención de muestras difícil. Por tanto, se ha cons… Leer más

Desarrollo y puesta a punto de una técnica de base inmunológica para la detección conjunta de ricina y aglutinina

Virginia Colmena García defenderó este trabajo para la obtención del Máster Universitario en Gestión de Desastres

La ricina es una de las toxinas más potentes conocidas, que se encuentra en la semilla del ricino (Ricinus communis). El Centro para el Control y la Prevención de Enfermedades de Estados Unidos (CDC) la clasifica como posible arma biológica. Existen diferentes métodos para la detección de esta proteína, siendo la técnica inmunológica ELISA, especialmente el ELISA tipo sándwich, la que está considerada como la técnica más robusta para su análisis, ya que es sensible, específica, con límites de detección de picogramos y fácil de realizar. No obstante, el reto está en poder diferenciar la ricina … Leer más

Circulation of a novel strain of dolphin morbillivirus (DMV) in stranded cetaceans in the Mediterranean Sea

Artículo de investigación publicado en Scientific reports

Dolphin morbillivirus (DMV) has been responsible for several outbreaks of systemic infection and has resulted in cetacean strandings in the Mediterranean. In August-October 2016, seven striped dolphins (Stenella coeruleoalba) stranded on the Sicilian coastline (Italy) tested positive for DMV. Tissue samples from brain, lung, pulmonary lymph nodes, heart, spleen, liver, stomach, intestine, kidneys and urinary bladder, as well as blowhole swabs, were collected during necropsy for molecular diagnostics and pathology studies. Extracted tissue RNA was screened for DMV by real-time reverse transcrip… Leer más

Nucleotide sequence variations may be associated with virulence of deformed wing virus

Investigación publicada en Apidologie

Western honey bees (Apis mellifera) are key players in crop pollination and in the maintenance of global biodiversity. Their viability is threatened by Varroa destructor, which acts as a vector of the deformed wing virus (DWV). Several genetic DWV variants have been reported, but it is unclear whether their virulence differs. We examined the prevalence of V. destructor and DWV as well as bee health in two colonies over 21 months and then characterizing DWV variants from each colony using phylogenetics. Colony H showed no signs of disease or mortality, and DWV sequence from this colony clustere… Leer más

Evaluación de la situación sanitaria de las principales especies de cultivo acuícola en Papúa Nueva Guinea

Tesis Doctoral que será defendida por Ruth García Gómez en el Centro VISAVET de la Universidad Computense de Madrid

Desde hace mas de dos décadas se ha tratado de promover y de desarrollar el sector acuícola en la Región de Oceanía de manera económica y técnicamente viable. Numerosas agencias y programas internacionales, intergubernamentales y regionales han focalizados sus esfuerzos económicos y humanos en el desarrollo sostenible de este sub-sector dentro del sector pesquero en Oceanía, con resultados ciertamente limitados. La región de Oceanía sigue siendo la zona del mundo con el menor grado de desarrollo del sector acuícola del mundo, con algunas excepciones como Australia y Nueva Zelya, seguida de reg… Leer más

Effect of litter aggregation and pooling on detection of porcine reproductive and respiratory virus in piglet processing fluids

Investigación publicada en Journal of Veterinary Diagnostic Investigation

A sampling technique has been validated to monitor porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) using the serosanguinous exudate known as processing fluids (PFs) that accumulate from tissues obtained during tail docking and castration. PFs are an aggregate sample of large numbers of piglets and litters. However, little is known about the effect of litter aggregation on the ability of PCR to correctly classify an aggregated PF sample as positive. We evaluated both the effect of litter aggregation and of PF pooling on PCR detection. We estimated that aggregation of at least 50… Leer más

The possible route of introduction of bluetongue virus serotype 3 into Sicily by windborne transportation of infected Culicoides spp

Artículo de investigación publicado en Transboundary and Emerging Diseases

In October 2017, the first outbreak of bluetongue virus serotype 3 (BTV-3) began in Italy, specifically in western Sicily. The route of entrance remains unclear, although since 2016 the same strain had been circulating only 150 km away, on the Tunisian peninsula of Cape Bon. The present analysis assessed the feasibility that wind could have carried BTV-3-infected Culicoides spp. from Tunisia to Sicily. An advection-deposition-survival (ADS) model was used to estimate when and where Culicoides spp. were likely to be introduced prior to the first BTV-3 report in Italy. Additionally, the Hybrid S… Leer más

Potentially human-virulent Vibrio vulnificus isolates from diseased great pompano (Trachinotus goodei)

Transboundary and Emerging Diseases publica este artículo de investigación

Vibrio vulnificus is an opportunistic human pathogen responsible for the majority of seafood-associated deaths worldwide and is also a relevant fish pathogen for the aquaculture industry. In addition to infections in aquatic livestock, Vibrio vulnificus also represents a risk to aquarium animals. For the first time, this work describes an important mortality outbreak in Trachinotus goodei in a zoo aquarium, with the isolation of Vibrio vulnificus (Vv) from the internal organs of the diseased fish. The isolates were identified by MALDI-TOF MS, serotyped and characterized by PFGE. Although the i… Leer más