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Últimas noticias de Investigación
14 de febrero de 2019
Vía publiDB
Comparison of individual, group and environmental sampling strategies to conduct influenza surveillance in pigs
BMC Veterinary Research publica este artículo de investigación
BACKGROUND:
Influenza A virus (IAV) is an important pathogen in pigs that affects productivity and has important public health implications because of its zoonotic nature. Surveillance is central to the control of influenza, however, detection of IAV infections can be challenging in endemically infected herds with low prevalence of infection.
METHODS:
In groups of suckling (18-21 days of age) and growing (35-45 days of age) pigs, we compared various sampling approaches to detect, isolate and sequence IAV using individual (nasal swabs, nasal wipes and oropharyngeal swabs), grou…
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3 de febrero de 2019
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Day-old chicks are of source of antimidrobial resistant bacteria for laying hen farms
Investigación publicada en Veterinary Microbiology
Antimicrobial resistant bacteria are rarely detected in laying hens and the objective of this longitudinal study was to test day-old chick as a source. Four different commercial batches raised on the same farm were monitored from day-old chick to laying hens using Escherichia coli as a model. Ten colonies from each of the eight samplings per batch were tested for antimicrobial susceptibility using 14 antimicrobials.
Overall (313 isolates), higher resistance percentages were detected for tetracycline (26.8%), followed by sulphonamides (16.3%), ampicillin (16.0 %) and quinolones (10.9% and 9.3%…
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1 de febrero de 2019
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A new test to detect antibodies against Mycobacterium tuberculosis complex in red deer serum
The Veterinary Journal publica este artículo de investigación
Red deer (Cervus elaphus) farming is a growing economic activity worldwide. However, the capacity of this species to act as reservoir of animal tuberculosis (TB) poses a threat to other wildlife and to livestock. Diagnostic assay accuracy in this species is therefore highly relevant for prevention and control measures. Our aim was to evaluate the diagnostic performance of the protein complex P22, obtained from Mycobacterium bovis derived purified protein derivative (bPPD), as a candidate antigen for the detection of antibodies against Mycobacterium tuberculosis complex (MTC). We assessed the p…
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25 de enero de 2019
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Spatio-temporal trends in the frequency of interspecific interactions between domestic and wild ungulates from Mediterranean Spain
Artículo de investigación publicado en PLoS ONE
Controlling infections shared by wildlife and livestock requires the understanding and quantification of interspecific interactions between the species involved. This is particularly important in extensive multi-host systems, in which controlled domestic animals interact with uncontrolled, abundant and expanding wild species, such as wild ungulates. We have, therefore, quantified the interspecific interactions between wild boar (Sus scrofa) and free-ranging cattle in Mediterranean Spain, along with their spatio-temporal variability. GPS-GSM-collars were used to monitor 12 cows and 14 wild boar…
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18 de enero de 2019
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New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers
Artículo de investigación publicado en Veterinary Medicine and Science
European badgers (Meles meles) have been identified as wildlife reservoirs for Mycobacterium bovis in the UK and Ireland, and may also have a role in the epidemiology of animal tuberculosis in other European regions. Thus, detection of M. bovis-infected badgers may be required for the purposes of surveillance and monitoring of disease levels in infected populations. Current serological assays to detect M. bovis infection in live badgers, while rapid and inexpensive, show limited diagnostic sensitivity. Here we describe and evaluate new ELISA platforms for the recognition of the P22 multiprotei…
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3 de enero de 2019
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Revisiting area risk classification of visceral leishmaniasis in Brazil
Artículo de investigación publicado en BMC infectious diseases
Background: Visceral leishmaniasis (VL) is a neglected tropical disease of public health relevance in Brazil. To prioritize disease control measures, the Secretaria de Vigilância em Saúde of Brazil’s Ministry of Health (SVS/MH) uses retrospective human case counts from VL surveillance data to inform a municipality-based risk classification. In this study, we compared the underlying VL risk, using a spatiotemporal explicit Bayesian hierarchical model (BHM), with the risk classification currently in use by the Brazil’s Ministry of Health. We aim to assess how well the current risk classes captur…
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1 de enero de 2019
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Validation of a new serological assay for the identification of Mycobacterium tuberculosis complex-specific antibodies in pigs and wild boar
Investigación publicada en Preventive Veterinary Medicine
Background and objectives
Animal tuberculosis (TB) is a multi-host disease involving a wide variety of domestic and wild mammals and causing a significant economic burden and sanitary problems. Wild boar and domestic pigs (Sus scrofa) are indicators of the circulation of the Mycobacterium tuberculosis complex (MTC) and can play a role in its maintenance. The proper diagnosis of MTC contact in these species is, therefore, a key factor as regards controlling TB. The objective of the current study is to evaluate the diagnostic performance of the protein complex P22 as a candidate for use in an i…
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1 de enero de 2019
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Assessment of reproducibility of a VP7 Blocking ELISA diagnostic test for African horse sickness
Transboundary and Emerging Diseases publica este artículo de investigación
The laboratory diagnosis of African horse sickness (AHS) is important for: (a) demonstrating freedom from infection in a population, animals or products for trade (b) assessing the efficiency of eradication policies; (c) laboratory confirmation of clinical diagnosis; (d) estimating the prevalence of AHS infection; and (e) assessing postvaccination immune status of individual animals or populations. Although serological techniques play a secondary role in the confirmation of clinical cases, their use is very important for all the other purposes due to their high throughput, ease of use and good…
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19 de diciembre de 2018
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Aplicación de herramientas cuantitativas para el estudio epidemiológico de zoonosis
Tesis Doctoral defendida por Mª Luisa de la Cruz Conty en la Facultad de Veterinaria de la Universidad Complutense de Madrid
En la presente tesis doctoral se ha explorado la utilidad de la aplicación de técnicas de análisis cuantitativo para el estudio de la epidemiología de enfermedades de relevancia en sanidad animal y salud pública.
La mayor parte de estudios epidemiológicos se fundamentan en la clasificación de los individuos de la población en las categorías de infectado y no infectado en función de los resultados de una prueba diagnóstica. No obstante, con frecuencia la fiabilidad de esa prueba no se evalúa previamente, o bien dicha evaluación tiene lugar en unas circunstancias no aplicables posteriormente …
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14 de diciembre de 2018
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Caracterización inmunoproteómica de derivados proteicos de Mycobacterium bovis, Mycobacterium avium (subespecies avium y paratuberculosis) y Corynebacterium pseudotuberculosis. Aplicación diagnóstica
Jose Antonio Infantes Lorenzo defendió su tesis doctoral en la Facultad de Veterinaria de la Universidad Complutense de Madrid
La tuberculosis animal es una zoonosis bacteriana producida por bacterias del complejo Mycobacterium tuberculosis, especialmente M. bovis y M. caprae que representa un importante problema sanitario en el hombre y los animales (domésticos y salvajes). Por esta razón, en la Unión Europea, los bovinos están sometidos a un programa de erradicación que se basa en el “diagnóstico y sacrificio” de los animales reaccionantes a las pruebas de diagnóstico oficiales, cuyo reactivo básico es la tuberculina bovina o PPDb. Esta es un derivado proteico purificado de M. bovis de composición y titulación compl…
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