Últimas publicaciones científicas ISI
Antibody detection tests improve the sensitivity of tuberculosis diagnosis in cattle
Research In Veterinary Science 112(2017):214-221.
We evaluated the sensitivity (Se) of the single cervical intradermal tuberculin (SIT) test, two interferon-gamma (IFN-γ) assays and three different antibody detection techniques for bovine tuberculosis (bTB) diagnosis in 131 mixed beef breed cattle. The results of the diagnostic techniques performed over the whole herd, and over the animals confirmed as infected based on the presence of lesions compatible with the disease and/or M. bovis isolation were compared to determine apparent prevalence (AP) and Se.
The Se of the SIT test (severe interpretation) was 63.7% (95% CI, 54.54–72.00), while the Se of the IFN-γ assays ranged between 60.2% and 92%. The proportion of infected cattle detected by the different antibody detection… Leer más
Casal C., Infantes-Lorenzo JA., Risalde MA., Diez-Guerrier A., Dominguez M., Moreno I., Romero B., de Juan L., Saez JL., Juste R., Gortazar C., Dominguez L. y Bezos J..
First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis
Journal Of Equine Veterinary Science 53(2017):30-37.
Strangles is one of the most frequently diagnosed equine respiratory infectious diseases in the world. It is caused by Streptococcus equi subsp. equi (S. equi), and it is an acute infection characterized by pyrexia, nasal discharge, pharyngitis, and abscessation of lymph nodes. Frequently, healthy horses might continue to harbor S. equi after clinical recovery. Although the genetic distance between S. equi isolates is short, strains can be differentiated by pulsed-field gel electrophoresis (PFGE) and single locus sequence typing for epidemiological studies. The aim of this study was to characterize by PFGE Argentine isolates of S. equi obtained from horses with acute strangles and those that had recovered. Bacterial isolation and identifica… Leer más
Bustos CP., Guida N., Casamayor A., Munoz AJ., Fernandez-Garayzabal JF. y Vela AI..
Human influence and biotic homogenization drive the distribution of Escherichia coli virulence genes in natural habitats
MicrobiologyOpen 6(3):1-10.
Cattle are the main reservoirs for Shiga-toxin-producing Escherichia coli (STEC), the only known zoonotic intestinal E. coli pathotype. However, there are other intestinal pathotypes that can cause disease in humans, whose presence has been seldom investigated. Thus, our aim was to identify the effects of anthropic pressure and of wild and domestic ungulate abundance on the distribution and diversity of the main human E. coli pathotypes and nine of their representative virulence genes (VGs). We used a quantitative real-time PCR (qPCR) for the direct detection and quantification of the genus-specific gene uidA, nine E. coli VGs (stx1, sxt2, eae, ehxA, aggR, est, elt, bfpA, invA), as well as four genes related to O157:H7 (rfbO157 , fliCH7 ) a… Leer más
Cabal A., Vicente J., Alvarez J., Barasona JA., Boadella M., Dominguez L. y Gortazar C.
Highly Tigecycline-Resistant Klebsiella pneumoniae Sequence Type 11 (ST11) and ST147 Isolates from Companion Animals
Antimicrobial Agents And Chemotherapy 61(6). pii: e02640-16.
In this study we have characterized two tigecycline resistant Klebsiella pneumoniae clones isolated from dog urine samples. The isolates were genetically unrelated, belonging to sequence types ST11 and ST147, both classically related to human isolates. To the best of our knowledge, this is the first identification of tigecycline resistant isolates from animals. We unveil the worrisome circulation among animals of bacterial clones resistant to this last resort antibiotic… Leer más
Ovejero CM., Escudero JA., Thomas-Lopez D., Hoefer A., Moyano G., Montero N., Martin-Espada C. y Gonzalez-Zorn B..
Jeotgalibaca porci sp. nov. and Jeotgalibaca arthritidis sp. nov., two new species isolated from pigs and emended description of the genus Jeotgalibaca
International Journal Of Systematic And Evolutionary Microbiology 67(5):1473-1477.
Biochemical and molecular genetic studies were performed on two novel Gram-positive catalase-negative coccus-shaped organisms isolated from liquid joint samples of two pigs. The microorganisms were not identified as a recognized species based on their cellular, morphological and biochemical tests. 16S rRNA gene sequence comparison studies allowed their identifications as members of the genus Jeotgalibaca, but the organisms were different to Jeotgalibaca dankookensis, the single species of the genus. The two microorganisms shared 96.3% and 96.9% 16S rRNA gene sequence similarity values with their nearest phylogenetic relative, J. dankookensis. The novel bacterial isolates were distinguished from J. dankookensis using biochemical tests. Based… Leer más
Vela AI., Perez-Sancho M., Zamora L., Dominguez L. y Fernandez-Garayzabal JF..
Phylogenomic analysis of the complete sequence of a gastroenteritis-associated cetacean adenovirus (bottlenose dolphin adenovirus 1) reveals a high degree of genetic divergence
Infection Genetics And Evolution S1567-1348(17)30159-4.
Adenoviruses are common pathogens in vertebrates, infecting a wide range of hosts, but only having rarely been detected and correlated with disease in cetaceans. This article describes the first complete genomic sequence of a cetacean adenovirus, bottlenose dolphin adenovirus 1 (BdAdV-1), detected in captive bottlenose dolphin population (Tursiops truncatus) suffering from self-limiting gastroenteritis. The complete genome sequence of BdAdV-1 was recovered from data generated by high-throughput sequencing and validated by Sanger sequencing. The genome is 34,080bp long and has 220 nucleotides long inverted terminal repeats. A total of 29 coding sequences were identified, 26 of which were functionally annotated. Among the unusual features of … Leer más
Malmberg M., Rubio-Guerri C., Hayer J., Garcia-Parraga D., Nieto-Pelegrin E., Melero M., Alvaro T., Valls M., Sanchez-Vizcaino JM., Belak S. y Granberg F.
Tuberculin Skin Testing Boosts Interferon-gamma Responses to DIVA Reagents in Mycobacterium bovis-infected Cattle
Clinical And Vaccine Immunology 24(5). pii: e00551-16.
BCG vaccination sensitises cattle to bovine tuberculin which compromises the use of the current bovine TB surveillance tests. Although the performance of a blood test (that utilises antigens expressed by Mycobacterium bovis but not by BCG) capable of discriminating infected from vaccinated animals (DIVA interferon-gamma test, DIT) has been evaluated in naturally infected TB field reactors, there is a need to perform similar analysis in a BCG vaccinated/M. bovis infected population. Furthermore, we explored different scenarios under which a DIT may be implemented alongside BCG vaccination: (i) serial testing to resolve potential false positive skin test results; or (ii) standalone test to replace the single intradermal comparative cervical t… Leer más
Jones GJ., Coad M., Khatri B., Bezos J., Parlane NA., Buddle BM., Villareal-Ramos B., Hewinson RG. y Vordermeier M.
Characterization of Pasteurella multocida associated with ovine pneumonia using multi-locus sequence typing (MLST) and virulence-associated gene profile analysis and comparison with porcine isolates
Veterinary Microbiology 204:180-187.
Pasteurella multocida is a pathogen causing disease in a wide range of hosts including sheep and pigs. Isolates from ovine pneumonia were characterized by MLST (Multi-host and RIRDC databases) and virulence-associated gene (VAG) typing and compared with porcine isolates. Ovine and porcine isolates did not share any STs as determined by both schemes and exhibited different VAG profiles. With the Multi-host database, sixteen STs were identified among 43 sheep isolates with two STs (ST50 and ST19) comprising 53.5% of the isolates, and seven MLST genotypes (ST3, ST11 and ST62 included 75% of the isolates) among the 48 pig isolates. The most frequent VAG profile among sheep isolates was tbpA+/toxA+ (69.8% of isolates) and pfhA+ (62.5%) and hgbB+… Leer más
Garcia-Alvarez A., Vela AI., San Martin E., Chaves F., Fernandez-Garayzabal JF., Dominguez L. y Cid D.
In-Depth Characterization and Functional Analysis of Clonal Variants in a Mycobacterium tuberculosis Strain Prone to Microevolution
Frontiers In Microbiology (2017)8:694.
The role of clonal complexity has gradually been accepted in infection by Mycobacterium tuberculosis (MTB), although analyses of this issue are limited. We performed an in-depth study of a case of recurrent MTB infection by integrating genotyping, whole genome sequencing, analysis of gene expression and infectivity in in vitro and in vivo models. Four different clonal variants were identified from independent intrapatient evolutionary branches. One of the single-nucleotide polymorphisms in the variants mapped in mce3R, which encodes a repressor of an operon involved in virulence, and affected expression of the operon. Competitive in vivo and in vitro co-infection assays revealed higher infective efficiency for one of the clonal variants. A … Leer más
Navarro Y., Perez-Lago L., Herranz M., Sierra O., Comas I., Sicilia J., Bouza E. y Garcia de Viedma D.
Spread of mcr-1-carrying Enterobacteriaceae in sewage water from Spain
The Journal Of Antimicrobial Chemotherapy 72(4):1050-1053.
OBJECTIVES:
The mobile colistin resistance gene mcr-1 has been identified worldwide in human and animal sources, while its occurrence in the environment is still largely unknown. The aim of this study was to investigate the presence of mcr-1-harbouring Enterobacteriaceae in water samples obtained from rivers and waste water treatment plants in the area of Barcelona, Spain.
METHODS:
The presence of mcr-1 was detected by PCR. Bacterial identification was performed via MALDI-TOF MS. Resistance to colistin was determined by a broth dilution method. The epidemiological relationship between the positive isolates was assessed with PFGE and ST was determined by MLST. Plasmid characterization was performed by transformation expe… Leer más
Ovejero CM., Delgado-Blas JF., Calero-Caceres W., Muniesa M. y Gonzalez-Zorn B..
