Últimas publicaciones científicas SCI
Spoligotype-specific risk of finding lesions in tissues from cattle infected by Mycobacterium bovis
BMC Veterinary Research 17(1):148.
Although the pathogenic effect of members of the Mycobacterium tuberculosis complex in susceptible hosts is well known, differences in clinical signs and pathological findings observed in infected animals have been reported, likely due to a combination of host and pathogen-related factors. Here, we investigated whether Mycobacterium bovis strains belonging to different spoligotypes were associated with a higher risk of occurrence of visible/more severe lesions in target organs (lungs and/or lymph nodes) from infected animals. A large collection of 8889 samples belonging to cattle were classified depending on the presence/absence of tuberculosis-like lesions and its degree of severity. All samples were subjected to culture irrespective of th… Leer más
Gómez-Buendía A., Romero B., Bezos J., Lozano F., de Juan L. y Alvarez J..
Polyresistant Mycobacterium bovis Infection in Human and Sympatric Sheep, Spain, 2017-2018
Emerging Infectious Diseases 10.3201/eid2704.204467.
The main etiologic agent of tuberculosis (TB) in livestock is Mycobacterium bovis; human TB cases caused by M. bovis are rare. Analysis of a TB outbreak caused by polyresistant M. bovis involving a human and sympatric sheep in Spain suggests local circulation of drug-resistant M. bovis strains among livestock… Leer más
Perez del Val B., Romero B., Tortola MT., Herrera-Leon L., Pozo P., Mercader I., Saez JL., Domingo M. y Vidal E.
Evaluation of the performance of slaughterhouse surveillance for bovine tuberculosis detection in Castilla y Leon, Spain
Preventive Veterinary Medicine 189:105307.
Post-mortem inspection (PMI) of routinely slaughtered cattle in abattoirs is an extremely valuable tool for detecting bovine tuberculosis (bTB) infected herds that can supplement active surveillance activities. However, its true performance is difficult to assess due to the multiple factors that may affect it. Here, we determined relative efficiencies in the detection of bTB-compatible lesions and probabilities of subsequent laboratory confirmation of abattoirs located in Castilla y Leon, one of the regions with the largest cattle population in Spain, between 2010 and 2017. The slaughtered animal population was split based on the results of the ante-mortem tests (reactors or non-reactors), and two generalized linear multivariable mixed mode… Leer más
Pozo P., Cespedes-Cardenas N., Bezos J., Romero B., Grau A., Nacar J., Saez JL., Minguez O. y Alvarez J..
Towards a Better and Harmonized Education in Antimicrobial Stewardship in European Veterinary Curricula
Antibiotics 10(4):364.
Education in antimicrobial stewardship (AMS) in veterinary medicine is essential to foster responsible antimicrobial use and control of antimicrobial resistance (AMR) in animals. AMS is listed by the EU and international organizations among the basic `Day One Competences` required of veterinary students upon graduation. Our aim was to evaluate the quality of education of European veterinary students in AMS. We distributed a 27-item survey addressing the perceptions of preparedness and acquired skills on key topics related to AMS to final-year veterinary students in Europe. We collected 3423 complete answers from 89 veterinary schools in 30 countries. Selection of treatment strategies and awareness of emerging AMR problems were markedly diff… Leer más
Espinosa-Gongora C., Rem Jessen L., James-Dyar O., Bousquet-Melou A., Gonzalez-Zorn B., Pulcini C., Re G., Schwarz S., Timofte D., Toutain PL., Guardabassi L. y PREPARE-VET Working Group.
Transmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmer
Antibiotics 10(3):313.
Colistin use has mostly been stopped in human medicine, due to its toxicity. However, nowadays, it still is used as a last-resort antibiotic to treat hospital infections caused by multi-drug resistant Enterobacteriaceae. On the contrary, colistin has been used in veterinary medicine until recently. In this study, 210 fecal samples from pigs (n = 57), calves (n = 152), and the farmer (n = 1) were collected from a farm where E. coli harboring mcr-1–mcr-3 was previously detected. Samples were plated, and mcr-genes presence was confirmed by multiplex-PCR. Hybrid sequencing which determined the presence and location of mcr-1, other antibiotic resistance genes, and virulence factors. Eighteen colistin resistant isolates (13 from calves, four from… Leer más
Vines J., Cusco A., Napp S., Alvarez J., Saez Llorente JL., Rosas-Rodoreda M., Francino O. y Migura-Garcia L.
High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge
Vaccines 9:242.
Abstract: African swine fever (ASF) is currently the major concern of the global swine industry, as a consequence of which a reconsideration of the containment and prevention measures taken to date is urgently required. A great interest in developing an effective and safe vaccine against ASF virus (ASFV) infection has, therefore, recently appeared. The objective of the present study
is to test an inactivated ASFV preparation under a vaccination strategy that has not previously been tested in order to improve its protective effect. The following have been considered: (i) virus inactivation by using a low binary ethyleneimine (BEI) concentration at a low temperature, (ii) the use of new and strong adjuvants; (iii) the use of very high … Leer más
Cadenas-Fernández E., Sanchez-Vizcaino JM., van den Born E., Kosowska A., van Kilsdonk E., Fernandez-Pacheco P., Gallardo C., Arias M. y Barasona JA..
Globetrotting strangles: the unbridled national and international transmission of Streptococcus equi between horses
Microbial Genomics 00.
The equine disease strangles, which is characterized by the formation of abscesses in the lymph nodes of the head and neck, is one of the most frequently diagnosed infectious diseases of horses around the world. The causal agent, Streptococcus equi subspecies equi , establishes a persistent infection in approximately 10 % of animals that recover from the acute disease. Such ‘carrier’ animals appear healthy and are rarely identified during routine veterinary examinations pre-purchase or transit, but can transmit S. equi to naïve animals initiating new episodes of disease. Here, we report the analysis and visualization of phylogenomic and epidemiological data for 670 isolates of S. equi recovered from 19 different countries using a new … Leer más
Mitchell C., Steward KF., Charbonneau ARL., Walsh S., Wilson H., Timoney JF., Wernery U., Joseph M., Craig D., van Maanen K., Hoogkamer-van Gennep A., Leon A., Witkowski L., Rzewuska M., Stefanska I., Zychska M., van Loon G., Cursons R., Patty O., Acke E., Gilkerson JR., El-Hage C., Allen J., Bannai H., Kinoshita Y., Niwa H., Becu T., Pringle J., Guss B., Bose R., Abbott Y., Katz L., Leggett B., Buckley TC., Blum SE., Cruz F., Fernadez-Ros A., Marotti Campi MC., Preziuso S., Robinson C., Newton JR., Schofield E., Brooke B., Boursnell M., de Brauwere N., Kirton R., Barton CK., Abudahab K., Taylor B., Yeats CA., Goater R., Aanensen DM., Harris SR., Parkhill J., Holden MTG. y Waller AS.
Impact of nurse sows on influenza A virus transmission in pigs under field conditions
Preventive Veterinary Medicine 188:105257.
Piglets prior to weaning play a central role in maintaining influenza infections in breeding herds and the use of nurse sows is a common practice to adopt piglets that fall behind and that otherwise would die. Transmission of influenza A virus (IAV) from nurse sows to adopted pigs has been reported experimentally, however, the importance of this route of transmission under field conditions has not yet been elucidated. A cohort study to assess the IAV status in nurse and control sows and their respective litters was carried out in three influenza positive breed-to-wean farms. A total of 94 control and 90 nurse sows were sampled by collecting udder skin wipes and oral swabs at enrollment (∼ 5–7 days after farrowing) and at weaning. Six … Leer más
Garrido Mantilla J., Sanhueza J., Alvarez J., Culhane M.R., Davies P., Allerson M.W. y Torremorel M.
Efficacy of a Salmonella enterica serovar Abortusovis (S. Abortusovis) inactivated vaccine in experimentally infected gestating ewes
Research In Veterinary Science 135:486-494.
Salmonella enterica serovar Abortusovis (S. Abortusovis) infection is one of the most important causes of infectious late-term abortion as well as birth of weak lambs in sheep in many countries throughout the world. Implementation of protocols based on the application of effective vaccines is one of the most effective approaches for controlling this disease, but variable efficacy has been reported, possibly related to factors associated with the host, the vaccine, the parameters used for determining efficacy and the challenge protocols. In this context, a new commercial inactivated vaccine (INMEVA; Laboratorios Hipra S.A., Spain) was evaluated in 20 control and 17 vaccinated gestating ewes, subcutaneously challenged at 90 days of gest… Leer más
Garcia-Seco T., Montbrau C., Fontseca M., March R., Sitja M., Dominguez L. y Bezos J..
Assessing red deer hunting management in the Iberian Peninsula: the importance of longitudinal studies
PeerJ 00.
Understanding the dynamics of a wildlife population in relation to hunting strategies is essential to achieve sustainable management. We used monitoring data over 25 years from two red deer (Cervus elaphus) populations with different management (with and without supplemental feeding) in South Central Spain to: (i) characterise the density dependence of population dynamics under contrasted management, and (ii) provide the basis for sustainable extraction by considering the theoretical maximum sustainable yield (MSYt) as the reference. The red deer population displayed a typical management reactive culling approach (‘saw-tooth-like’ curves), with occasional strong annual harvests but not occurring on a regular basis. Interestingly, we found r… Leer más
Carpio-Camargo AJ., Barasona JA., Acevedo O., Fierro Y., Gortazar C., Vigal C., Moreno A. y Vicente J.
