Últimas publicaciones científicas SCI
The bla NDM-1 carrying IncA/C2 plasmid underlies structural alterations and co-integrate formation in vivo
Antimicrobial Agents And Chemotherapy 63(8).
In 2012, a carbapenemase-producing Salmonella (S.) Corvallis carrying a bla NDM-1 multi-resistance IncA/C2 plasmid, apart from IncHI2 and ColE-like plasmids, was detected in a wild bird in Germany. In recent broiler chicken infection study, we observed transfer of this bla NDM-1-carrying IncA/C2 plasmid to other Enterobacteriaceae Here, we focused on the stability of this plasmid and gained insight into type and frequency of its structural alterations after an in vivo passage in a broiler infection study… Leer más
Hadziabdic S., Fischer J., Borowiak M., Malorny B., Juraschek K., Kasbohrer A., Guerra B., Deneke C., Gonzalez-Zorn B. y Szabo I.
Circulation of a novel strain of dolphin morbillivirus (DMV) in stranded cetaceans in the Mediterranean Sea
Scientific Reports 9:9792.
Dolphin morbillivirus (DMV) has been responsible for several outbreaks of systemic infection and has resulted in cetacean strandings in the Mediterranean. In August-October 2016, seven striped dolphins (Stenella coeruleoalba) stranded on the Sicilian coastline (Italy) tested positive for DMV. Tissue samples from brain, lung, pulmonary lymph nodes, heart, spleen, liver, stomach, intestine, kidneys and urinary bladder, as well as blowhole swabs, were collected during necropsy for molecular diagnostics and pathology studies. Extracted tissue RNA was screened for DMV by real-time reverse transcription polymerase chain reaction (PCR). Some tissues exhibited microscopic lesions that were consistent with DMV infection on histopathological and immu… Leer más
Mira F., Rubio-Guerri C., Purpari G., Puleio R., Caracappa G., Gucciardi F., Russoto L., Ruggero-Loria G. y Guercio A.
Nucleotide sequence variations may be associated with virulence of deformed wing virus
Apidologie 1-15.
Western honey bees (Apis mellifera) are key players in crop pollination and in the maintenance of global biodiversity. Their viability is threatened by Varroa destructor, which acts as a vector of the deformed wing virus (DWV). Several genetic DWV variants have been reported, but it is unclear whether their virulence differs. We examined the prevalence of V. destructor and DWV as well as bee health in two colonies over 21 months and then characterizing DWV variants from each colony using phylogenetics. Colony H showed no signs of disease or mortality, and DWV sequence from this colony clustered with VDV/DWV-B sequences previously reported in healthy colonies. Colony W showed DWV symptoms, and DWV sequence clustered with DWV-A sequences prev… Leer más
Barroso-Arevalo S., Vicente-Rubiano M., Molero F., Puerta F. y Sanchez-Vizcaino JM..
Effect of litter aggregation and pooling on detection of porcine reproductive and respiratory virus in piglet processing fluids
Journal Of Veterinary Diagnostic Investigation 31(4):625-628.
A sampling technique has been validated to monitor porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) using the serosanguinous exudate known as processing fluids (PFs) that accumulate from tissues obtained during tail docking and castration. PFs are an aggregate sample of large numbers of piglets and litters. However, little is known about the effect of litter aggregation on the ability of PCR to correctly classify an aggregated PF sample as positive. We evaluated both the effect of litter aggregation and of PF pooling on PCR detection. We estimated that aggregation of at least 50 litters was possible when a pig with a Ct value of ~22 was present in the sample, and aggregation of up to 40 litters was possible when there was a s… Leer más
Vilalta C., Baker J., Sanhueza J., Murray D., Sponheim A., Alvarez J., Sylvia F., Polson D., Torremorell M., Corzo C. y Morrison RB.
The possible route of introduction of bluetongue virus serotype 3 into Sicily by windborne transportation of infected Culicoides spp
Transboundary And Emerging Diseases 66(4):1665-1673.
In October 2017, the first outbreak of bluetongue virus serotype 3 (BTV-3) began in Italy, specifically in western Sicily. The route of entrance remains unclear, although since 2016 the same strain had been circulating only 150 km away, on the Tunisian peninsula of Cape Bon. The present analysis assessed the feasibility that wind could have carried BTV-3-infected Culicoides spp. from Tunisia to Sicily. An advection-deposition-survival (ADS) model was used to estimate when and where Culicoides spp. were likely to be introduced prior to the first BTV-3 report in Italy. Additionally, the Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model was used to support ADS outputs. The modelling suggests that during September 2017, st… Leer más
Aguilar-Vega C., Fernandez-Carrion E. y Sanchez-Vizcaino JM..
Potentially human-virulent Vibrio vulnificus isolates from diseased great pompano (Trachinotus goodei)
Transboundary And Emerging Diseases 66(4):1765-1770.
Vibrio vulnificus is an opportunistic human pathogen responsible for the majority of seafood-associated deaths worldwide and is also a relevant fish pathogen for the aquaculture industry. In addition to infections in aquatic livestock, Vibrio vulnificus also represents a risk to aquarium animals. For the first time, this work describes an important mortality outbreak in Trachinotus goodei in a zoo aquarium, with the isolation of Vibrio vulnificus (Vv) from the internal organs of the diseased fish. The isolates were identified by MALDI-TOF MS, serotyped and characterized by PFGE. Although the isolates from great pompanos did not belong to pathovar piscis (formerly biotype 2) or to any of the fish-related serovars, they all had identical phen… Leer más
Gibello A., Vela AI., Martinez-Nevado E., Rodriguez-Bertos A., Casamayor A., Garcia J., Dominguez L., Montoto P., Fernandez-Garayzabal JF. y Amaro C.
Host Richness Increases Tuberculosis Disease Risk in Game-Managed Areas
Microorganisms 7(6):182.
Current scientific debate addresses whether species richness in animal communities may negatively moderate pathogen transmission and disease outcome (dilution effect), or to the contrary, if disease emergence benefits from more diverse community assemblages (amplification effect). The result may not depend exclusively on patterns of host species biodiversity but may depend on the specific composition of reservoir hosts and vectors, and their ecology. Host–pathogen interactions have shaped variations in parasite virulence, transmissibility and specificity. In the same way the importance of factors related to host exposure or to life history trade-offs are expected to vary. In this study, we demonstrate that ungulate host species richness cor… Leer más
Barasona JA., Gortazar C., de la Fuente J. y Vicente J.
Weaned piglets: another factor to be considered for the control of Salmonella infection in breeding pig farms
Veterinary Research 50:45.
Field studies on Salmonella infection in suckling piglets are scarce due to the intrinsic difficulties of collecting proper samples (i.e. tonsils or mesenteric lymph nodes), and most of them rely on the analysis of rectal swabs that limit their accuracy. We used 495 slaughtered 4-weeks-old male piglets intended for human consumption from 5 Salmonella-seropositive breeding farms to collect gastrointestinal packages and perform a thorough detection of Salmonella on mesenteric lymph nodes and intestinal content. The overall prevalence of both infection and shedding was high (≈ 36%) indicating that piglets played an active role in Salmonella maintenance in the farms. Major serotypes found in piglets included 4,[5],12:i: (35.4%), Ris… Leer más
Casanova-Higes A., Marin-Alcala CM., Andres-Barranco S., Cebollada-Solanas A., Alvarez J. y Mainar-Jaime RC.
High Load of Deformed Wing Virus and Varroa destructor Infestation Are Related to Weakness of Honey Bee Colonies in Southern Spain
Frontiers In Microbiology (10)1331, 1-8.
Many factors, including pathogens, contribute to the continuing losses of colonies of the honey bee Apis mellifera, which has led to steady population decline. In particular, colony losses have been linked to deformed wing virus (DWV) and the Varroa destructor mite. To clarify the potential role of these two pathogens in honey bee colony weakening and loss, we sampled colonies in southern Spain during a 21-month period and analyzed the samples for loads of four viruses and varroa. Loads of DWV and black queen cell virus as well as varroa infestation negatively correlated with colony vigor as measured using the subjective colony strength method. Logistic regression identified varroa and DWV as the main factors involved in colony weakening. O… Leer más
Barroso-Arevalo S., Fernandez-Carrion E., Goyache J., Molero F., Puerta F. y Sanchez-Vizcaino JM..
Estimating day range from camera-trap data: the animals’ behaviour as a key parameter
Journal Of Zoology 1-9.
Day range (DR), the distance travelled by an individual during the day, is an important metric in movement ecology that recently gained interest by its relevance for estimating population density through the random encounter model (REM). Traditionally, DR has been estimated using GPS technology and considering raw straight-line distances between consecutive locations, which is an underestimation of the true path distance. In this work, we tested the accuracy of a new approach based on camera-trap data for the estimation of DR taking into account the animals’ behaviour. For this purpose, we considered wild boar (Sus scrofa) as a model species. We tagged 18 individuals with telemetry devices and then monitored the population with camera-traps… Leer más
Palencia P., Vicente J., Barroso P., Barasona JA., Soriguer RC. y Acevedo P.
