Últimas publicaciones científicas ISI
Escherichia coli ST167 carrying plasmid mobilisable mcr-1 and blaCTX-M-15 resistance determinants isolated from a human respiratory infection
International Journal Of Antimicrobial Agents 50(2):285-286.
The emergence of polymyxin resistance in Enterobacteriaceae is a matter of major concern and has instigated the recommendation for its surveillance since it compromises the use of colistin, a last-resort antibiotic.… Leer más
Sanchez-Benito R., Iglesias MR., Quijada NM., Campos MJ., Ugarte-Ruiz M., Hernandez M., Pazos C., Rodriguez-Palenzuela P., Garduno E., Dominguez L. y Quesada A.
Oral Vaccination with Heat-Inactivated Mycobacterium bovis Does Not Interfere with the Antemortem Diagnostic Techniques for Tuberculosis in Goats
Frontiers In Veterinary Science 4(124):1-7.
Vaccination against tuberculosis (TB) is prohibited in cattle or other species subjected to specific TB eradication campaigns, due to the interference that it may cause with the official diagnostic tests. However, immunization with a heat-inactivated (HI) Mycobacterium bovis vaccine via the oral route has been suggested to overcome this issue. In this study, the main goal was to assess the interference of the HI vaccine by different routes of administration using a previous vaccination and re-vaccination (boosting) protocol. TB-free kid goats were divided into three groups: oral (n = 16), intramuscular (IM; n = 16), and control (n = 16). Results showed that there was a significant difference in the percentage of animals positive to the sing… Leer más
Roy A., Risalde MA., Casal C., Romero B., de Juan L., Menshawy AMS., Diez-Guerrier A., Juste R., Garrido JM., Sevilla I., Gortazar C., Dominguez L. y Bezos J..
Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015
Eurosurveillance 22(31):3.
Colistin resistance genes mcr-3 and mcr-1 have been detected in an Escherichia coli isolate from cattle faeces in a Spanish slaughterhouse in 2015. The sequences of both genes hybridised to same plasmid band of ca 250 kb, although colistin resistance was non-mobilisable. The isolate was producing extended-spectrum beta-lactamases and belonged to serotype O9:H10 and sequence type ST533. Here we report an mcr-3 gene detected in Europe following earlier reports from Asia and the United States.… Leer más
Hernandez M., Iglesias MR., Rodriguez-Lazaro D., Gallardo A., Quijada NM., Miguela-Villoldo P., Campos MJ., Piriz S., Lopez G., De Frutos C., Saez JL., Ugarte-Ruiz M., Dominguez L. y Quesada A.
Effect of seafood peptones on biomass and metabolic activity by Enterococcus faecalis DM19
LWT - Food Science And Technology 81(2017):94-100.
Eight seafood protein hydrolysates (SPHs) obtained from squid, shrimp and fish gelatin were incorporated as substitutes of peptones in culture media in order to evaluate its effect on survival and metabolic activity (lactic acid, acetic acid and bacteriocins production) of Enterococcus faecalis DM19. The substitution of commercial peptones in culture media by either a shrimp hydrolysate prepared with Protamex, or by squid protein hydrolysates prepared with Esperase or Alkaline protease, stimulated E. faecalis DM19 growth up to 16%. The incorporation of SPHs, mainly from shrimp, in the culture media significantly increased production of lactic and acetic acids in more than 60%. Furthermore, the media containing SPHs stimulated antimicrobial … Leer más
Djellouli M., Martinez-Alvarez O., Arancibia MY., Florez-Cuadrado D., Ugarte-Ruiz M., Dominguez L., Zadi-Karam H., Karam N. y Roudj S.
Serological Surveillance and Direct Field Searching Reaffirm the Absence of Ornithodoros Erraticus Ticks Role in African Swine Fever Cycle in Sardinia
Transboundary And Emerging Diseases 64(4):1322-1328.
African swine fever (ASF), one of the most important diseases of swine, has been endemic in the Italian island of Sardinia for more than 35 years. During these decades, several strategies and eradication efforts have been implemented in the island with limited success. Strong climatic and ecological similarities exist between Sardinia and one area of the Iberian Peninsula where Ornithodoros erraticus ticks were involved in the persistence of ASF from 1960 to 1995. This fact leads to the hypothesis that, potentially, Ornithodoros ticks could be also involved in the ASF cycle in Sardinia, thus accounting for some of the reoccurring ASF outbreaks in this island. Initial efforts aimed at detection of Ornithodoros ticks in Sardinia were performe… Leer más
Mur L., Iscaro C., Cocco M., Jurado C., Rolesu S., de Mia GM., Feliziani F., Perez-Sanchez R., Oleaga A. y Sanchez-Vizcaino JM..
Insights into a novel blaKPC-2 -encoding IncP-6 plasmid reveal carbapenem-resistance circulation in several Enterobacteriaceae species from wastewater and a hospital source in Spain
Frontiers In Microbiology 8:1143.
Untreated wastewater, particularly from hospitals and other healthcare facilities, is considered to be a reservoir for multidrug-resistant bacteria. However, its role in the spread of antibiotic resistances in the human population remains poorly investigated. We used whole genome sequencing (WGS) to analyze 25 KPC-2-producing Enterobacteriaceae isolates from sewage water collected during a 3-year period and three clinical Citrobacter freundii isolates from a tertiary hospital in the same collection area in Spain. We detected a common, recently described, IncP-6 plasmid carrying the gene blaKPC-2 in 21 isolates from both sources. The plasmid was present in diverse environmental bacterial species of opportunistic pathogens such as C. freundii… Leer más
Yao Y., Lazaro-Perona F., Falgenhauer L., Valverde A., Imizalioglu C., Dominguez L., Canton R., Mingorance J. y Chakraborty T.
Antibody detection tests improve the sensitivity of tuberculosis diagnosis in cattle
Research In Veterinary Science 112(2017):214-221.
We evaluated the sensitivity (Se) of the single cervical intradermal tuberculin (SIT) test, two interferon-gamma (IFN-γ) assays and three different antibody detection techniques for bovine tuberculosis (bTB) diagnosis in 131 mixed beef breed cattle. The results of the diagnostic techniques performed over the whole herd, and over the animals confirmed as infected based on the presence of lesions compatible with the disease and/or M. bovis isolation were compared to determine apparent prevalence (AP) and Se.
The Se of the SIT test (severe interpretation) was 63.7% (95% CI, 54.54–72.00), while the Se of the IFN-γ assays ranged between 60.2% and 92%. The proportion of infected cattle detected by the different antibody detection… Leer más
Casal C., Infantes-Lorenzo JA., Risalde MA., Diez-Guerrier A., Dominguez M., Moreno I., Romero B., de Juan L., Saez JL., Juste R., Gortazar C., Dominguez L. y Bezos J..
First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis
Journal Of Equine Veterinary Science 53(2017):30-37.
Strangles is one of the most frequently diagnosed equine respiratory infectious diseases in the world. It is caused by Streptococcus equi subsp. equi (S. equi), and it is an acute infection characterized by pyrexia, nasal discharge, pharyngitis, and abscessation of lymph nodes. Frequently, healthy horses might continue to harbor S. equi after clinical recovery. Although the genetic distance between S. equi isolates is short, strains can be differentiated by pulsed-field gel electrophoresis (PFGE) and single locus sequence typing for epidemiological studies. The aim of this study was to characterize by PFGE Argentine isolates of S. equi obtained from horses with acute strangles and those that had recovered. Bacterial isolation and identifica… Leer más
Bustos CP., Guida N., Casamayor A., Munoz AJ., Fernandez-Garayzabal JF. y Vela AI..
Human influence and biotic homogenization drive the distribution of Escherichia coli virulence genes in natural habitats
MicrobiologyOpen 6(3):1-10.
Cattle are the main reservoirs for Shiga-toxin-producing Escherichia coli (STEC), the only known zoonotic intestinal E. coli pathotype. However, there are other intestinal pathotypes that can cause disease in humans, whose presence has been seldom investigated. Thus, our aim was to identify the effects of anthropic pressure and of wild and domestic ungulate abundance on the distribution and diversity of the main human E. coli pathotypes and nine of their representative virulence genes (VGs). We used a quantitative real-time PCR (qPCR) for the direct detection and quantification of the genus-specific gene uidA, nine E. coli VGs (stx1, sxt2, eae, ehxA, aggR, est, elt, bfpA, invA), as well as four genes related to O157:H7 (rfbO157 , fliCH7 ) a… Leer más
Cabal A., Vicente J., Alvarez J., Barasona JA., Boadella M., Dominguez L. y Gortazar C.
Highly Tigecycline-Resistant Klebsiella pneumoniae Sequence Type 11 (ST11) and ST147 Isolates from Companion Animals
Antimicrobial Agents And Chemotherapy 61(6). pii: e02640-16.
In this study we have characterized two tigecycline resistant Klebsiella pneumoniae clones isolated from dog urine samples. The isolates were genetically unrelated, belonging to sequence types ST11 and ST147, both classically related to human isolates. To the best of our knowledge, this is the first identification of tigecycline resistant isolates from animals. We unveil the worrisome circulation among animals of bacterial clones resistant to this last resort antibiotic… Leer más
Ovejero CM., Escudero JA., Thomas-Lopez D., Hoefer A., Moyano G., Montero N., Martin-Espada C. y Gonzalez-Zorn B..
