Últimas publicaciones científicas SCI
Treatment with the senolytics dasatinib/quercetin reduces SARS-CoV-2-related mortality in mice
Aging Cell e13771.
The enormous societal impact of the ongoing COVID-19 pandemic has been particularly harsh for some social groups, such as the elderly. Recently, it has been suggested that senescent cells could play a central role in pathogenesis by exacerbating the pro-inflammatory immune response against SARS-CoV-2. Therefore, the selective clearance of senescent cells by senolytic drugs may be useful as a therapy to ameliorate the symptoms of COVID-19 in some cases. Using the established COVID-19 murine model K18-hACE2, we demonstrated that a combination of the senolytics dasatinib and quercetin (D/Q) significantly reduced SARS-CoV-2-related mortality, delayed its onset, and reduced the number of other clinical symptoms. The increase in senescent markers… Leer más
Pastor-Fernandez A., Rodriguez-Bertos A., Sierra-Ramirez A., del Moral-Salmoral J., Merino J., de Avila AI., Olague C., Villares R., Gonzalez-Aseguinolaza G., Rodriguez MA., Fresno M., Girones N., Bustos M., Smerdou C., Fernandez-Marcos PJ. y von Kobbe C.
Engineered live bacteria suppress Pseudomonas aeruginosa infection in mouse lung and dissolve endotracheal-tube biofilms
Nature Biotechnology 000.
Engineered live bacteria could provide a new modality for treating lung infections, a major cause of mortality worldwide. In the present study, we engineered a genome-reduced human lung bacterium, Mycoplasma pneumoniae, to treat ventilator-associated pneumonia, a disease with high hospital mortality when associated with Pseudomonas aeruginosa biofilms. After validating the biosafety of an attenuated M. pneumoniae chassis in mice, we introduced four transgenes into the chromosome by transposition to implement bactericidal and biofilm degradation activities. We show that this engineered strain has high efficacy against an acute P. aeruginosa lung infection in a mouse model. In addition, we demonstrated that the engineered strain could dissolv… Leer más
Mazzolini R., Rodriguez-Arce I., Fernandez-Barat L., Pinero-Lambea C., Garrido V., Rebollada A., Motos A., Torres A., Grillo MJ., Serrano L. y Lluch-Senar M.
Characterization of the Fecal and Mucosa-Associated Microbiota in Dogs with Chronic Inflammatory Enteropathy
Animals 13:326.
Chronic inflammatory enteropathies are the most common cause of chronic vomiting and diarrhea in dogs. The pathogenesis of this disease is known to be multifactorial, where intestinal barrier dysfunction, immunological dysregulation and gut microbiota changes play a central role. Most sequencing studies assessing the intestinal microbiota in canine species have been made to evaluate fecal samples. Therefore, in this study, we aimed to characterize the intestinal bacterial microbiota from duodenal biopsies and fecal samples in dogs with inflammatory bowel disease at the diagnosis time and to compare it to healthy dogs. Our study results demonstrate that
dogs with inflammatory bowel disease have significantly different gut microbiota w… Leer más
Diaz-Reganon D., Garcia-Sancho M., Villaescusa A., Sainz A., Agulla B., Reyes-Prieto M., Rodriguez-Bertos A. y Rodriguez-Franco F.
Struggling to improve farm biosecurity: Do free advice and subsidies hit the target?
Preventive Veterinary Medicine 212:105839.
Biosafety measures (BSMs) often aim at reducing the likelihood of cross-species interactions at the wildlife-livestock interface. Examples include means to segregate wild ungulates from cattle at waterholes or at feeders. Subsidies or incentives for BSM implementation are expected to contribute to improved BSM acceptance. However, several recent experiences led us to write a cautionary commentary on the variable success of incentives in farm biosafety promotion. We list examples where, after offering farm-specific biosecurity action plans for free or subsidizing 100% of the cost of a given BSM, 25-40% of the farmers remained unwilling to invest efforts in farm biosafety and BSM maintenance. We suggest seeking a better understanding of farme… Leer más
Preite L., Barroso P., Romero B., Balseiro A. y Gortazar C.
What about the bull? A systematic review about the role of males in bovine infectious infertility within cattle herds
Veterinary And Animal Science 19:100284.
Numerous pathogens affect cow fertility. Nevertheless, little information has been published about microorganisms associated with cattle infertility focusing on bulls. The present review offers a current analysis and highlights potential key aspects on the relevance of bulls in the emergence of infertility problems of infectious origin within herds that are still not completely determined. The present systematic review was conducted using the PubMed, Web of Science, and Scopus databases on December 9, 2022. In total, 2,224 bibliographic records were reviewed and, according to strict inclusion criteria, 38 articles were selected from 1966 to 2022, from which we ranked more than 27 different microorganisms (fungi were not identified). The mos… Leer más
Polo C., Garcia-Seco T., Diez-Guerrier A., Briones V., Dominguez L. y Perez-Sancho M..
First report and molecular characterization of cases of natural Taylorella asinigenitalis infection in three donkey breeds in Spain
Veterinary Microbiology 276:109604.
Taylorella asinigenitalis is a non-pathogenic bacteria isolated from the genital tract of donkeys but also a cause of metritis and vaginal discharge in mares. It is closely related to Taylorella equigenitalis, the cause of Contagious Equine Metritis (CEM) in horses, and has been present in different countries in Europe since 1995. Up to date, there are no studies on the prevalence of T. asinigenitalis in the equine or asinine populations in Spain; this is the first report of the presence of T. asinigenitalis in donkeys (Equus asinus) from different breeds in three regions of Spain. A total of 106 healthy animals of three different Spanish donkey breeds: Andaluza (26), Majorera (12) and Zamorano-Leonés (68) were sampled between June and July… Leer más
Dorrego-Rodriguez A., Herranz-Benito C., Perez-Sancho M., Camino E., Gomez-Arrones V., Carrasco JJ., de Gabriel-Perez J., Serres C. y Cruz F..
Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks
Animals 13(1):75.
Studies that characterize bovine respiratory disease (BRD)-associated Pasteurella multocida isolates are scarce compared with research on isolates from other hosts and clinical backgrounds. In the present study, 170 P. multocida isolates from 125 BRD outbreaks were characterized by capsular and LPS typing as well as by virulotyping. Three capsular types (A, B, F) and three LPS genotypes (L2, L3, L6) were identified. Capsular and LPS typing revealed a very low genetic diversity (GD = 0.02) among P. multocida, with most isolates belonging to genotype A:L3 (97.6%). Virulotyping identified seven virulence-associated gene profiles, with two profiles including 95.9% of the isolates. A subset of isolates was further characterized by MLST and PFGE.… Leer más
Calderón-Bernal JM., Fernandez A., Arnal JL., Sanz-Tejero C., Fernandez-Garayzabal JF., Vela AI. y Cid D.
A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats
Scientific Reports 12(1):22132.
This study aimed to assess the efficacy of a heat-inactivated Mycobacterium caprae (HIMC) vaccine in goats experimentally challenged with the same strain of M. caprae. Twenty-one goats were divided into three groups of seven: vaccinated with heat-inactivated Mycobacterium bovis (HIMB), with HIMC and unvaccinated. At 7 weeks post-vaccination all animals were endobronchially challenged with M. caprae. Blood samples were collected for immunological assays and clinical signs were recorded throughout the experiment. All goats were euthanized at 9 weeks post-challenge. Gross pathological examination, analysis of lung pathology using computed tomography, and bacterial load quantification in pulmonary lymph nodes (LN) by qPCR were carried out. Only… Leer más
Melgarejo C., Planas C., Cobos A., Arrieta-Villegas C., Sevilla IA., Bezos J., Moll X., Espada Y., Garrido JM., Domingo M., Vidal E. y Perez del Val B.
Genomics, Transcriptomics, and Metabolomics Reveal That Minimal Modifications in the Host Are Crucial for the Compensatory Evolution of ColE1-Like Plasmids
MSphere 7(6):e0018422.
Plasmid-mediated antimicrobial resistance is one of the major threats to public health worldwide. The mechanisms involved in the plasmid/host coadaptation are still poorly characterized, and their understanding is crucial to comprehend the genesis and evolution of multidrug-resistant bacteria. With this purpose, we designed an experimental evolution using Haemophilus influenzae RdKW20 as the model strain carrying the ColE1-like plasmid pB1000. Five H. influenzae populations adapted previously to the culture conditions were transformed with pB1000 and subsequently evolved to compensate for the plasmid-associated fitness cost. Afterward, we performed an integrative multiomic analysis combining genomics, transcriptomics, and metabolomics to ex… Leer más
Ares M., Fernandez-Garcia M., Wedel E., Montero N., Barba C., Fernanda Rey-Stolle M., García A. y Gonzalez-Zorn B..
Environmental factors driving fine-scale ixodid tick abundance patterns
Science Of The Total Environment 853:158633.
Tick abundance is an essential demographic parameter to infer tick-borne pathogen transmission risks. Spatiotemporal patterns of tick abundance are heterogeneous, so its determinants at small spatial scales need to be understood to reduce their negative effects on hosts. Current knowledge of these determinants is scarce, especially in Mediterranean environments, limiting the possibilities for designing efficient tick control strategies. With the goal of unravelling tick abundance determinants and informing new tick management strategies, we estimated tick burdens on 1965 wild ungulates in Doñana National Park, Spain, annually between 2010 and 2020. Under the hypothesis of a predominant host influence on tick abundance, we modelled the burde… Leer más
Peralbo-Moreno A., Baz-Flores S., Cuadrado-Matias R., Barroso P., Triguero-Ocana R., Jiménez-Ruiz S., Herraiz C., Ruiz-Rodriguez C., Acevedo P. y Ruiz-Fons F.
