Últimas publicaciones científicas SCI
Tuberculosis Epidemiology and Badger (Meles meles) Spatial Ecology in a Hot-Spot Area in Atlantic Spain
Pathogens 8(4)292.
We provide a temporal overview (from 2012 to 2018) of the outcomes of tuberculosis (TB) in the cattle and badger populations in a hot-spot in Asturias (Atlantic Spain). We also study the badger’s spatial ecology from an epidemiological perspective in order to describe hazardous behavior in relation to TB transmission between cattle and badgers. Culture and single intradermal tuberculin test (SITT) were available for cattle as part of the National Program for the Eradication of TB. A field survey was also carried out in order to determine the paddocks and buildings used by each farm, and the information obtained was stored by using geographic information systems. Moreover, eighty-three badgers were submitted for necropsy and subsequent bacte… Leer más
Acevedo P., Prieto M., Quiros P., Merediz I., de Juan L., Infantes-Lorenzo JA., Triguero-Ocana R. y Balseiro A.
Association between results of diagnostic tests for bovine tuberculosis and Johne`s disease in cattle
The Veterinary Record 31554708.
BACKGROUND:
Bovine tuberculosis (bTB) diagnosis is impaired by numerous factors including cross-reactivity with Mycobacterium avium subspecies paratuberculosis, which causes Johne`s disease (JD). In addition, the effect of repeated bTB-intradermal testing on the performance of JD diagnostic tests is not fully understood. This study aimed to evaluate the impact of repeated bTB-intradermal tests under field conditions in Spain on the JD serological status of cattle.
METHODS:
bTB-positive herds (n=264) from Castilla-y-Leon region were selected and matched with officially tuberculosis-free control herds. The association between JD and bTB status at the herd level was assessed using conditional logistic regression an… Leer más
Picasso C., Grau A., Bakker D., Nacar J., Minguez O., Perez A. y Alvarez J..
Use of a voluntary testing program to study the spatial epidemiology of Johne’s disease affecting dairy herds in Minnesota: a cross sectional study
BMC Veterinary Research 15(1):429.
One of the key steps in the management of chronic diseases in animals including Johne’s disease (JD), caused by Mycobacterium avium subsp. paratuberculosis (MAP), is the ability to track disease incidence over space and time. JD surveillance in the U.S. dairy cattle is challenging due to lack of regulatory requirements, imperfect diagnostic tests, and associated expenses, including time and labor. An alternative approach is to use voluntary testing programs. Here, data from a voluntary JD testing program, conducted by the Minnesota Dairy Herd Improvement Association, were used to: a) explore whether such a program provides representative information on JD-prevalence in Minnesota dairy herds, b) estimate JD distribution, and, c) identify her… Leer más
Kanankege KST., Machado G., Zhang L., Dokkebakken B., Schumann V., Wells SJ., Perez AM. y Alvarez J..
Assessment of the sensitivity of the bovine tuberculosis eradication program in a high prevalence region of Spain using scenario tree modeling
Preventive Veterinary Medicine 173:104800.
In spite of the efforts invested to eradicate bovine tuberculosis (bTB) in cattle, the disease is still present in several developed countries, including Spain. Among the factors related with disease persistence in high prevalence areas, the lack of sensitivity of the screening test for detection of infected herds [single intradermal tuberculin (SIT) test] can play a major role. Here, a scenario tree model mimicking the diagnostic test scheme in place in the region of Castilla y Leon (Spain) was developed to estimate the probability of detecting bTB in an infected-non detected officially tuberculosis free (OTF) herd (herd sensitivity, HSe). In order to do so the probability of detecting at least one positive animal in the SIT test with/with… Leer más
de la Cruz ML., Pozo P., Grau A., Nacar J., Bezos J., Perez A., Dominguez L., Saez JL., Minguez O., de Juan L. y Alvarez J..
Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease
Environmental Microbiology 21(12):4597-4613.
The use of antimicrobials in human and veterinary medicine has coincided with a rise in antimicrobial resistance (AMR) in the food‐borne pathogens Campylobacter jejuni and Campylobacter coli. Faecal contamination from the main reservoir hosts (livestock, especially poultry) is the principal route of human infection but little is known about the spread of AMR among source and sink populations. In particular, questions remain about how Campylobacter resistomes interact between species and hosts, and the potential role of sewage as a conduit for the spread of AMR. Here we investigate the genomic variation associated with AMR in 168 C. jejuni and 92 C. coli strains isolated from humans, livestock and urban effluents in Spain. Antimicrobia… Leer más
Mourkas E., Florez-Cuadrado D., Pascoe B., Calland JK., Bayliss SC., Mageiros L., Meric G., Hitchings MD., Quesada A., Porrero MC., Ugarte-Ruiz M., Gutierrez-Fernandez J., Dominguez L. y Sheppard SK.
A Summer Mortality Outbreak of Lactococcosis by Lactococcus garvieae in a Raceway System Affecting Farmed Rainbow Trout (Oncorhynchus mykiss) and Brook Trout (Salvelinus fontinalis)
Animals (Basel) 9(12).
Lactococcosis is a fish disease of major concern in Mediterranean countries caused by Lactococcus garvieae. The most susceptible species is the rainbow trout (Oncorhynchus mykiss), suffering acute disease associated with elevated mortalities compared to other fish species. References reported that other salmonids are also susceptible to the disease, but no mortality outbreak has been described to date. The aim of this study was to present a mortality outbreak that occurred in brook trout (Salvelinus fontinalis) farmed in northwestern Italy during the summer of 2018. Fish exhibited clinical signs, such as exophthalmos, diffused hemorrhages localized in the ocular zone, hemorrhagic enteritis, and enlarged spleen. L. garvieae was isolated in a… Leer más
Pastorino P., Vela AI., Colussi S., Cavazza G., Menconi V., Mugetti D., Righetti M., Barbero R., Zuccaro G., Fernandez-Garayzabal JF., Dondo A., Acutis PL. y Prearo M.
Diarrheagenic Escherichia coli Pathotypes From Children Younger Than 5 Years in Kano State, Nigeria
Frontiers In Public Health 7:348.
Diarrheagenic Escherichia coli (DEC) is one of the leading causes of gastrointestinal disorders worldwide and an important public health challenge. DEC infection is often underdiagnosed during routine microbiological analysis, especially in resource constrained settings; the use of molecular tests could however help to determine the distribution of DEC and its clinical significance. Here, a study to assess the prevalence of DEC in clinical samples from patients <5 years attending three hospitals in Kano state, Nigeria, was carried out. Samples from 400 patients and 50 controls were collected and screened for E. coli. Compatible colonies from 248 individuals (215 patients and 33 controls) were characterized using biochemical test, a set of r… Leer más
Saka, K.H., Dabo NT., Muhammad B., Garcia-Soto S., Ugarte-Ruiz M. y Alvarez J..
Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration
Cell Metabolism S1550-4131(19)30562-5.
Life on Earth emerged in a hydrogen sulfide (H2S)-rich environment eons ago and with it protein persulfidation mediated by H2S evolved as a signaling mechanism. Protein persulfidation (S-sulfhydration) is a post-translational modification of reactive cysteine residues, which modulate protein structure and/or function. Persulfides are difficult to label and study due to their reactivity and similarity with cysteine. Here, we report a facile strategy for chemoselective persulfide bioconjugation using dimedone-based probes, to achieve highly selective, rapid, and robust persulfide labeling in biological samples with broad utility. Using this method, we show persulfidation is an evolutionarily conserved modification and waves of persulfidation … Leer más
Zivanovic J., Kouroussis E., Kohl JB., Adhikari B., Bursac B., Schott-Roux S., Petrovic D., Miljkovic JL., Thomas-Lopez D., Jung Y., Miler M., Mitchell S., Milosevic V., Gomes JE., Benhar M., Gonzalez-Zorn B., Ivanovic-Burmazovic I., Torregrossa R., Mitchell JR., Whiteman M., Schwarz G., Snyder SH., Paul BD., Carroll KS. y Filipovic MR.
Effects on Intestinal Mucosal Morphology, Productive Parameters and Microbiota Composition after Supplementation with Fermented Defatted Alperujo (FDA) in Laying Hens
Antibiotics 8(4):E215.
The olive oil sector is currently adapting its traditional function to also become a supplier of high-value by-products that possess antioxidant, anti-inflammatory and antimicrobial properties. In this study, we evaluated the effect of the fermented defatted alperujo (FDA) on the intestinal health of laying hens. The morphology of the duodenal and cecal mucosa, the composition of the intestinal
microbiota and the productivity of a batch of laying hens were evaluated after FDA supplementation. At early life stages, significant differences (p < 0.001) were observed in duodenal villi height and in crypt depth of both the duodenum and the cecum in the FDA-supplemented group, indicating improved intestinal health in this group. Microbiota… Leer más
Rebollada A., Barcena C., Ugarte-Ruiz M., Porras-Gonzalez N., Mayoral-Alegre F., Tome-Sanchez I., Dominguez L. y Rodriguez-Bertos A..
Coffee Silverskin Extract: Nutritional Value, Safety and Effect on Key Biological Functions
Nutrients 11(11).
This study aimed to complete the scientific basis for the validation of a coffee silverskin extract (CSE) as a novel food ingredient according to European legislation. Nutritional value, safety, effects on biochemical biomarkers and excretion of short chain fatty acids (SCFAs) in vivo of CSE were assessed. Proteins, amino acids, fat, fatty acids, fiber, simple sugars and micronutrients were analyzed. For the first time, toxicological and physiological effects were evaluated in vivo by a repeated-dose study in healthy Wistar rats. Hormone secretion, antioxidant (enzymatic and no-enzymatic) and anti-inflammatory biomarkers, and dietary fiber fermentability of CSE (analysis of SCFAs in feces) were studied in biological samples. This unique res… Leer más
Iriondo-Dehond A., Rios MB., Herrera T., Rodriguez-Bertos A., Nunez F., San Andres MI., Sanchez-Fortun S. y del Castillo MD.
