Últimas publicaciones científicas ISI
Exploring the oxidative, antimicrobial and genomic properties of Campylobacter jejuni strains isolated from poultry
Research In Veterinary Science 119(2018)170-175.
Campylobacter jejuni is the leading cause of food-borne bacterial enteritis in humans, with contaminated poultry products considered the main source of infection. To survive the food chain, C. jejuni utilizes multiple defense mechanisms that counter oxidative and aerobic stresses. In this study, we phenotypically characterised 63 C. jejuni strains with oxidative stress survival and antimicrobial susceptibility testing to investigate correlations between these two phenotypes against the source of the strains and the presence of the MarR regulators RrpA and RrpB which have a role in regulating the response to oxidative and aerobic stress. C. jejuni strains isolated from meat and neck skin displayed the highest resistance to oxidative stress. … Leer más
Ugarte-Ruiz M., Dominguez L., Corcionivoschi N., Brendan WW., Dorrell N. y Gundogdu O.
Detection of Toxoplasma gondii in three common bottlenose dolphins (Tursiops truncatus); A first description from the Eastern Mediterranean Sea
Veterinary Parasitology 258(2018)74-78.
Toxoplasma gondii has been described in several marine mammals around the world including numerous species of cetaceans, yet infection and transmission mechanisms in the marine environment are not clearly defined. The Israel Marine Mammal Research and Assistance Center has been collating a database of all marine mammal stranding events along the country’s national coastlines since 1993. In this study, we describe the molecular detection and characterisation of T. gondii in three common bottlenose dolphins (Tursiops truncatus) including one case of coinfection with herpesvirus. The animals were found stranded on the Mediterranean coast of Israel in May and November 2013. In one of the three cases, the dolphin was found alive and admitted to … Leer más
Bigal E., Morick D., Scheinin AP., Salant H., Berkowitz A., King R., Levy Y., Melero M., Sanchez-Vizcaino JM., Goffman O., Hadar N., Roditi-Elasar M. y Tchernov D.
Educating in antimicrobial resistance awareness: adaptation of the Small World Initiative program to service-learning
FEMS Microbiology Letters 00.
The Small World Initiative (SWI) is a consolidated and successful education program rooted in the USA that tackles the antibiotic crisis by a crowdsourcing strategy. Based on active learning, it challenges young students to discover novel bioactive-producing microorganisms form environmental soil samples. Besides its pedagogical efficiency to impart Microbiology contents in academic curricula, SWI promotes vocations on research and development in Experimental Sciences and, at the same time, disseminates the antibiotic awareness guidelines of the World Health Organization (WHO). We have adapted the SWI program to the Spanish academic environment by a pioneering hierarchic strategy based on service-learning that involves two education levels … Leer más
Valderrama MJ., Gonzalez-Zorn B., Calvo de Pablo P., Diez Orejas R., Fernandez Acero T., Gil Serna J., de Juan L., Martin H., Molina M., Navarro Garcia Belen Patiño F., Pla J., Prieto D., Rodriguez C., Roman E., Sanz Santamaría AB., de Siloniz MI., Suarez M., Vazquez C. y Cid VJ.
Human Pasteurella multocida Infection with Likely Zoonotic Transmission from a Pet Dog, Spain
Emerging Infectious Diseases 24(6):1145-1146.
We report a case of urinary tract infection caused by an unusual genotype (sequence type 211) of Pasteurella multocida associated with human infection. Molecular genetic analysis of P. multocida isolates obtained from the human patient and his pet strongly suggests a zoonotic transmission of this bacterium… Leer más
Abreu F., Rodriguez-Lucas C., Rodicio MR., Vela AI., Fernandez-Garayzabal JF., Leiva PS., Cuesta F., Cid D. y Fernandez J.
Impact of piglet oral vaccination against tuberculosis in endemic free-ranging wild boar populations
Preventive Veterinary Medicine 155(2018)11-20.
The Eurasian wild boar (Sus scrofa) is the main wild reservoir of the Mycobacterium tuberculosis complex in Mediterranean woodlands and a key risk factor for cattle tuberculosis (TB) breakdowns. Wild boar vaccination therefore has the potential to be a valuable tool for TB control. We tested two orally delivered vaccines, heat-inactivated Mycobacterium bovis (IV) and BCG, in four sites (two per vaccine type: one Managed and one Natural or unmanaged) during four years. TB was also monitored in 15 unvaccinated sites (spatial control), as well as in all sites from one year prior to intervention (temporal control). The rationale is that by vaccinating 2–6 month old wild boar piglets we can reduce disease at the population level during the study… Leer más
Díez Delgado I., Sevilla IA., Romero B., Tanner E., Barasona JA., White AR., Lurz PWW., Boots M., de la Fuente J., Dominguez L., Vicente J., Garrido JM., Juste RA., Aranaz A. y Gortazar C.
Evaluation of the use of a needle-free injection syringe as a cause of non-specific reactions in the intradermal tuberculin test used for the diagnosis of bovine tuberculosis
Research In Veterinary Science 119(2018):56-60.
The objective of the study was to elucidate whether the use of the needle-free Dermojet syringe, which is based on a high pressure inoculation and is used to inject tuberculin in cattle in several countries, may, in itself, cause skin reactions that can be interpreted as positive reactions to the intradermal tests that are not, in fact, related to the real infection status of the animals.
Forty-four cattle from an officially tuberculosis-free (OTF) herd were selected, and four single intradermal tuberculin (SIT) tests were performed on each animal, two on each side of the neck. Three different Dermojet (D1, D2 and D3) and one McLintock (M4) syringes were used to carry out sterile phosphate buffer saline (PBS) with 10% of glycerol and… Leer más
Diez-Guerrier A., Roy A., de la Cruz ML., Saez JL., Sanz C., Boschiroli L., Romero B., de Juan L., Dominguez L. y Bezos J..
Temporal analysis of the interference caused by paratuberculosis vaccination on the tuberculosis diagnostic tests in goats
Preventive Veterinary Medicine 156(2018)68-75.
Vaccination against paratuberculosis (PTB) in goats is a cost-effective control strategy, and is also effective as regards preventing the onset of clinical cases. However, it causes interference in the diagnostic tests used in the control of tuberculosis (TB). A group of 99 goats from a herd with no history of TB or PTB infection was vaccinated against PTB at seven months of age. They then underwent consecutive intradermal tests [single (SIT) and comparative (CIT) intradermal tuberculin tests), interferon-gamma release assays (IGRA) and two serological tests (p22_CE and DR-ELISA) every three months, until the interference disappeared. When using the SIT test, a variable number of positive reactors were observed at 3 months (T3; 32.3%, 95% C… Leer más
Roy A., Infantes-Lorenzo JA., Blazquez J., Venteo A., Mayoral-Alegre F., Dominguez M., Moreno I., Romero B., de Juan L., Grau A., Dominguez L. y Bezos J..
Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
Journal Of Visualized Experiments : JoVE (135).
Multicopy plasmids are extremely abundant in prokaryotes but their role in bacterial evolution remains poorly understood. We recently showed that the increase in gene copy number per cell provided by multicopy plasmids could accelerate the evolution of plasmid-encoded genes. In this work, we present an experimental system to test the ability of multicopy plasmids to promote gene evolution. Using simple molecular biology methods, we constructed a model system where an antibiotic resistance gene can be inserted into Escherichia coli MG1655, either in the chromosome or on a multicopy plasmid. We use an experimental evolution approach to propagate the different strains under increasing concentrations of antibiotics and we measure survival of ba… Leer más
Escudero JA., MacLean RC. y San Millan A.
The use of serological tests in combination with the intradermal tuberculin test maximizes the detection of tuberculosis infected goats
Veterinary Immunology And Immunopathology 199(2018):43-52.
The diagnosis of tuberculosis (TB) in goats is based mainly on the single and comparative intradermal tuberculin (SIT and CIT) tests and, exceptionally, on the interferon-gamma (IFN-γ) assay, however they are not perfect in terms of sensitivity and specificity. Nevertheless, various serological assays that provide a potential cost-effective approach for the control of TB are also available or under development, and a variety of results have been reported regarding the ability of these tests to detect infected animals, particularly in the early stages of infection.
In the present study, SIT/CIT and IFN-γ tests and three different serological assays were evaluated during two consecutive herd testing events in a recently infec… Leer más
Bezos J., Roy A., Infantes-Lorenzo JA., Gonzalez I., Venteo A., Romero B., Grau A., Minguez O., Dominguez L. y de Juan L..
Streptococcus penaeicida sp. nov., isolated from a diseased farmed Pacific white shrimp (Penaeus vannamei)
International Journal Of Systematic And Evolutionary Microbiology 68(5):1490-1495.
Strain CAIM 1838T, isolated from the hepatopancreas of a cultured diseased Pacific white shrimp (Penaeusvannamei), was subjected to characterization by a polyphasic taxonomic approach. On the basis of 16S rRNA gene sequence analysis, strain CAIM 1838T was most closely related to Streptococcus bovimastitidis 99.3 % and to other species of the Pyogenes clade of Streptococcus with lower similarity values. Average nucleotide identity values and the genome-to-genome distance of strain CAIM 1838T, as compared with the type strains, confirmed the separate species status with closely related species of the genus Streptococcus and were all below the thresholds to delimit a species, 93.1 and 49.4 %, respectively. The DNA G+C content… Leer más
Morales-Covarrubias MS., Del Carmen Bolan-Mejía M., Vela AI., Fernandez-Garayzabal JF. y Gomez-Gil B.
