Últimas publicaciones científicas SCI
A new test to detect antibodies against Mycobacterium tuberculosis complex in red deer serum
The Veterinary Journal 244(2019):98-103.
Red deer (Cervus elaphus) farming is a growing economic activity worldwide. However, the capacity of this species to act as reservoir of animal tuberculosis (TB) poses a threat to other wildlife and to livestock. Diagnostic assay accuracy in this species is therefore highly relevant for prevention and control measures. Our aim was to evaluate the diagnostic performance of the protein complex P22, obtained from Mycobacterium bovis derived purified protein derivative (bPPD), as a candidate antigen for the detection of antibodies against Mycobacterium tuberculosis complex (MTC). We assessed the performance of this new antigen in indirect enzyme-linked immunosorbent assays (ELISA) in TB-positive and TB-negative red deer, in comparison with a bP… Leer más
Thomas J., Infantes-Lorenzo JA., Moreno I., Romero B., Garrido F., Juste R., Dominguez M., Dominguez L., Gortazar C. y Risalde MA.
Spatio-temporal trends in the frequency of interspecific interactions between domestic and wild ungulates from Mediterranean Spain
PLoS ONE 14(1):e0211216.
Controlling infections shared by wildlife and livestock requires the understanding and quantification of interspecific interactions between the species involved. This is particularly important in extensive multi-host systems, in which controlled domestic animals interact with uncontrolled, abundant and expanding wild species, such as wild ungulates. We have, therefore, quantified the interspecific interactions between wild boar (Sus scrofa) and free-ranging cattle in Mediterranean Spain, along with their spatio-temporal variability. GPS-GSM-collars were used to monitor 12 cows and 14 wild boar in the Doñana National Park between 2011 and 2013. Interactions were defined as encounters between cattle and wild boar within a spatio-temporal wind… Leer más
Triguero-Ocana R., Barasona JA., Carro F., Soriguer RC., Vicente J. y Acevedo P.
New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers
Veterinary Medicine And Science (2019)1-9.
European badgers (Meles meles) have been identified as wildlife reservoirs for Mycobacterium bovis in the UK and Ireland, and may also have a role in the epidemiology of animal tuberculosis in other European regions. Thus, detection of M. bovis-infected badgers may be required for the purposes of surveillance and monitoring of disease levels in infected populations. Current serological assays to detect M. bovis infection in live badgers, while rapid and inexpensive, show limited diagnostic sensitivity. Here we describe and evaluate new ELISA platforms for the recognition of the P22 multiprotein complex derived from the purified protein derivative (PPD) of M. bovis. The recognition of IgG against P22 multiprotein complex derived from PPD-B w… Leer más
Infantes-Lorenzo JA., Dave D., Moreno I., Anderson P., Lesellier S., Gormley E., Dominguez L., Balseiro A., Gortazar C., Dominguez M. y Salguero FJ.
Revisiting area risk classification of visceral leishmaniasis in Brazil
BMC Infectious Diseases 19(1):2.
Background: Visceral leishmaniasis (VL) is a neglected tropical disease of public health relevance in Brazil. To prioritize disease control measures, the Secretaria de Vigilância em Saúde of Brazil’s Ministry of Health (SVS/MH) uses retrospective human case counts from VL surveillance data to inform a municipality-based risk classification. In this study, we compared the underlying VL risk, using a spatiotemporal explicit Bayesian hierarchical model (BHM), with the risk classification currently in use by the Brazil’s Ministry of Health. We aim to assess how well the current risk classes capture the underlying VL risk as modelled by the BHM.
Methods: Annual counts of human VL cases and the population at risk for all Brazil’s 5564 muni… Leer más
Machado G., Alvarez J., Bakka HC., Perez A., Donato LE., de Ferreira Lima Junior FE., Vieria Alves R. y del Rio Vilas VJ.
Validation of a new serological assay for the identification of Mycobacterium tuberculosis complex-specific antibodies in pigs and wild boar
Preventive Veterinary Medicine 162(2019):11-17.
Background and objectives
Animal tuberculosis (TB) is a multi-host disease involving a wide variety of domestic and wild mammals and causing a significant economic burden and sanitary problems. Wild boar and domestic pigs (Sus scrofa) are indicators of the circulation of the Mycobacterium tuberculosis complex (MTC) and can play a role in its maintenance. The proper diagnosis of MTC contact in these species is, therefore, a key factor as regards controlling TB. The objective of the current study is to evaluate the diagnostic performance of the protein complex P22 as a candidate for use in an in-house ELISA to identify M. tuberculosis complex-specific antibodies for the diagnosis of TB in comparison to the commonly used bPPD-based ELIS… Leer más
Thomas J., Infantes-Lorenzo JA., Moreno I., Cano-Terriza D., de Juan L., Garcia-Bocanegra I., Dominguez L., Dominguez M., Gortazar C. y Risalde MA.
Assessment of reproducibility of a VP7 Blocking ELISA diagnostic test for African horse sickness
Transboundary And Emerging Diseases 66:83–90.
The laboratory diagnosis of African horse sickness (AHS) is important for: (a) demonstrating freedom from infection in a population, animals or products for trade (b) assessing the efficiency of eradication policies; (c) laboratory confirmation of clinical diagnosis; (d) estimating the prevalence of AHS infection; and (e) assessing postvaccination immune status of individual animals or populations. Although serological techniques play a secondary role in the confirmation of clinical cases, their use is very important for all the other purposes due to their high throughput, ease of use and good cost-benefit ratio. The main objective of this study was to support the validation of AHS VP7 Blocking ELISA up to the Stage 3 of the World Animal He… Leer más
Duran-Ferrer M., Aguero M., Zientara S., Smith S., Potgieter C., Rueda P., Sastre P., Monaco F., Villalba R., Tena-Tomás C., Batten C., Frost L., Flannery J., Gubbins S., Lubisi BA., Sanchez-Vizcaino JM., Emery M., Sturgill T., Ostlund E. y Castillo-Olivares J.
Antimicrobial Prescriptions for Dogs in the Capital of Spain
Frontiers In Veterinary Science 5:309.
Objective: To characterize antimicrobial prescription patterns for dogs in veterinary practices in Spain using the city of Madrid as a model.
Design: Retrospective survey.
Settings: Dogs attending veterinary practices in the city of Madrid in 2017 were enrolled.
Subjects: Three hundred dogs from 30 veterinary practices randomly selected from a set of 388 practices grouped by zip code. The inclusion criterion for dogs was treatment with antibiotics within a few days of the data collection day.
Results: For the 300 dogs enrolled, 374 treatments with antimicrobials were recorded, 62.8% (235/374) were veterinary medicinal products and 37.2% (139/374) human medicinal products. The main route of administration was oral… Leer más
Gomez-Poveda B. y Moreno MA..
Spatio-temporal epidemiology of anthrax in Hippopotamus amphibious in Queen Elizabeth Protected Area, Uganda
PLoS ONE 13(11): e0206922.
Background
Anthrax is a zoonotic disease primarily of herbivores, caused by Bacillus anthracis, a bacterium with diverse geographical and global distribution. Globally, livestock outbreaks have declined but in Africa significant outbreaks continue to occur with most countries still categorized as enzootic, hyper endemic or sporadic. Uganda experiences sporadic human and livestock cases. Severe large-scale outbreaks occur periodically in hippos (Hippopotamus amphibious) at Queen Elizabeth Protected Area, where in 2004/2005 and 2010 anthrax killed 437 hippos. Ecological drivers of these outbreaks and potential of hippos to maintain anthrax in the ecosystem remain unknown. This study aimed to describe spatio-temporal patterns of anthrax… Leer más
Dicriu M., Rwego IB., Asiimwe B., Travis DA., Alvarez J., VanderWaal K. y Pelican K.
Evaluation of the McLintock syringe as a cause of non-specific reactions in the intradermal tuberculin test used for the diagnosis of bovine tuberculosis
Research In Veterinary Science 122(2019)175-178.
The objective of this study was to elucidate whether the use of the McLintock syringe, used to inject tuberculin in cattle in several countries and based on an intradermal inoculation by needle, may, in itself, cause skin reactions that can be interpreted as positive reactions regardless of the real tuberculosis (TB) infection status of the animals.
Forty-four cattle from an officially TB-free (OTF) herd were selected for the experiment. Each animal received four inoculations [one with sterile phosphate buffer saline (PBS) with 10% of glycerol and three with bovine purified protein derivative (PPD), as performed during the single intradermal tuberculin (SIT) test], two on each side of the neck (n=176 inoculations). Three different Mc… Leer más
Roy A., Diez-Guerrier A., Ortega J., de la Cruz ML., Saez JL., Dominguez L., de Juan L., Alvarez J. y Bezos J..
Development and characterization of mouse monoclonal antibodies to eight human complement components: analysis of reactivity with orthologs of nine mammalian genera
Comparative Immunology, Microbiology And Infectious Diseases 62(2019):7-12.
Abstract
To study complement function in mammalian leishmanioses, we developed mouse monoclonal antibodies to the human complement system components C1q, C4, factor D, factor H, factor B, properdin, C5 and C9. Antibody specificity was determined by indirect and capture ELISA and by Western blot. In flow cytometry analysis, seven antibodies recognized the cognate component on human serum-opsonized Leishmania promastigotes. Antibody reactivity was screened against promastigotes opsonized with sera of nine mammalian genera: pig, guinea pig, goat, rabbit, cat, dog, hamster, jird and rat. No antibody recognized jird epitopes on promastigotes. Anti-C4, -properdin, and -C5b reacted with the orthologous protein of all other mammals tested ex… Leer más
Monedero M., Infantes-Lorenzo JA., Moreno I., Martin AB., Macias RIR., Torano A. y Dominguez M.
