Últimas publicaciones científicas SCI
Long-Term Determinants of the Seroprevalence of the Hepatitis E Virus in Wild Boar (Sus scrofa)
Animals 11:1805.
The hepatitis E virus (HEV) is an emerging zoonotic pathogen whose main reservoir is suids. Most of the ecological and epidemiological aspects of its sylvatic cycle remain unknown. Thus, in this work, we study the drivers of HEV exposure in the wild boar population of Doñana National Park (DNP, southwest Spain) operating in the medium and long-term (2005–2018). Anti-HEV antibodies are widely distributed throughout the wild boar (46.7 ± 3.8%, 327 out of 700 sampled), showing a statistically significant age-increasing pattern. The temporal pattern displayed important interannual fluctuations. This could be mediated by marked variations in the population control of the wild boar, and subsequent changes in abundance rates, and its interplay wit… Leer más
Barroso P., Risalde MA., Garcia-Bocanegra I., Acevedo O., Barasona JA., Caballero-Gomez J., Jiménez-Ruiz S., Rivero-Juarez A., Montoro V. y Vicente J.
The Role of Interleukine-10 and Interferon-γ as Potential Markers of the Evolution of African Swine Fever Virus Infection in Wild Boar
Pathogens 10(6):757.
African swine fever virus (ASFv) is one of the most challenging pathogens to affect both domestic and wild pigs. The disease has now spread to Europe and Asia, causing great damage to the pig industry. Although no commercial vaccine with which to control the disease is, as yet, available, some potential vaccine candidates have shown good results in terms of protection. However, little is known about the host immune mechanisms underlying that protection, especially in wild boar, which is the main reservoir of the disease in Europe. Here, we study the role played by two cytokines (IL-10 and IFN-γ) in wild boar orally inoculated with the attenuated vaccine candidate Lv17/WB/Rie1 and challenged with a virulent ASFv genotype II isolate. A g… Leer más
Barroso-Arevalo S., Barasona JA., Cadenas-Fernández E. y Sanchez-Vizcaino JM..
Colistin selection of the mcr-1 gene in broiler chicken intestinal microbiota
Antibiotics 10:677.
: Colistin has a long story of safe use in animals for the treatment and prevention of certain bacterial diseases. Nevertheless, the first description of the mcr-1 gene showed that colistin resistance can spread by horizontal gene transfer and changed the landscape. This study aimed to assess the effect of colistin administration on the dispersion of resistance in the microbiota of day-old broiler chicks and how the presence of mcr-1 genes influences the spread of colistin resistance determinants. In this study, 100 one-day-old chicks were divided into four groups of 25 animals (G1, G2, G3, and G4). Animals from G3/G4 were challenged with mcr-1-carrying Salmonella (day 7), while colistin (600 mg/L) was administered daily to G2/G4 animals th… Leer más
Miguela-Villoldo P., Moreno MA., Rebollada A., Rodriguez-Bertos A., Hernandez M., Rodriguez-Lazaro D., Gallardo A., Quesada A., Goyache J., Dominguez L. y Ugarte-Ruiz M..
Expansion of native wild boar populations is a new threat for semi-arid wetland areas
Ecological Indicators 125:107563.
Wildlife management and conservation requires monitoring of species distribution and population indicators, especially when the unbalanced demographic changes of some species can affect the whole ecosystem functioning. The populations of wild boar (Sus scrofa) have, over the past few decades, undergone an expansion around the world, reaching situations of overabundance that can cause serious economic, ecological and health problems. This numerical increase of wild boar and its new spatial invasion can affect certain vulnerable species in sensitive ecological zones, such as the main inland wetland complex in SW Europe.
In this context, we aim to (i) examine the association of wild boar abundance and that of lagomorphs, and waterbird p… Leer más
Barasona JA., Carpio A., Boadella M., Gortazar C., Pineiro X., Zumalacarregui C., Vicente J. y Vinuela J.
Influence of multiple factors on hematologic reference intervals in horses residing in livery yards in Spain
Veterinary Clinical Pathology 1-5.
The hemogram is a routine analysis for equine veterinary practitioners in the assessment of patient clinical status. Reference intervals (RIs) of hematologic constituents vary according to different horse populations and are often described for a particular breed or horse type. The aims of this study were to determine RIs for hematologic constituents in a mixed-breed horse population residing in livery yards in central Spain and evaluate the associations between estimated RIs and multiple phenotypic and management characteristics. A total of 122 healthy horses from different breeds in central Spain were included in the study. RIs were calculated following the American Society for Veterinary Clinical Pathology guidelines. Significant associa… Leer más
Buendia A., Teng KT., Camino E., Dominguez L. y Cruz F..
Capsular type diversity of Mannheimia haemolytica determined by multiplex real-time PCR and indirect hemagglutination in clinical isolates from cattle, sheep, and goats in Spain
Veterinary Microbiology 258:109121.
This study compares the utility of a commercially available multiplex q-PCR assay for serotyping A1, A2, and A6 M. haemolytica serotypes with indirect hemagglutination, for determining the relative distribution of M. haemolytica capsular types associated with respiratory disorders in cattle, sheep, and goats. For the 129 isolates analyzed, both q-PCR and IHA assays exhibited nearly complete agreement for capsular types A1 (k = 0.965) and A2 (k = 0.888) and substantial agreement for A6 (k = 0.801). Despite the overall good performance of the commercial q-PCR, its effectiveness differed between the host origin of the isolates. The serotype was identified by q-PCR in 83.3 % of cattle, 77.8 % of goat, and 53.8 % of sheep isolates. Combining the… Leer más
Arnal JL., Fernandez A., Vela AI., Sanz C., Fernandez-Garayzabal JF. y Cid D.
Spatial and Temporal Distribution of Mycobacterium tuberculosis Complex Infection in Eurasian Badger (Meles meles) and Cattle in Asturias, Spain
Animals 11(5):1294.
The present work investigated the prevalence, spatial distribution, and temporal distribution of tuberculosis (TB) in free-ranging Eurasian badgers (Meles meles) and cattle in Asturias (Atlantic Spain) during a 13-year follow-up. The study objective was to assess the role of badgers as a TB reservoir for cattle and other sympatric wild species in the region. Between 2008 and 2020, 673 badgers (98 trapped and 575 killed in road traffic accidents) in Asturias were necropsied, and their tissue samples were cultured for the Mycobacterium tuberculosis complex (MTC) isolation. Serum samples were tested in an in-house indirect P22 ELISA to detect antibodies against the MTC. In parallel, data on MTC isolation and single intradermal tuberculin test … Leer más
Blanco-Vazquez C., Doria-Barral T., Romero B., Queipo M., Merediz I., Quiros P., Armenteros JA., Juste R., Dominguez L., Dominguez M., Casais R. y Balseiro A.
The plasmid-mediated Kluyvera-like arnBCADTEF operon confers colistin (hetero) resistance to Escherichia coli
Antimicrobial Agents And Chemotherapy 65(5):e00091-21.
The use of colistin as a last resort antimicrobial is compromised by the emergence of resistant enterobacteria with acquired determinants like mcr genes, mutations that activate the PmrAB system and by still unknown mechanisms. This work analyzed 74 E. coli isolates from healthy swine, turkey or bovine, characterizing their colistin resistance determinants. The mcr-1 gene, detected in 69 isolates, was the main determinant found among which 45% were carried by highly mobile plasmids, followed by four strains lacking previously known resistance determinants or two with mcr-4 (one in addition to mcr-1), whose phenotypes were not transferred by conjugation. Although a fraction of isolates carrying mcr-1 or mcr-4 genes also presented missense po… Leer más
Gallardo A., Iglesias M., Ugarte-Ruiz M., Hernandez M., Miguela-Villoldo P., Gutierrez G., Rodriguez-Lazaro D., Dominguez L. y Quesada A.
Population genomics and antimicrobial resistance dynamics of Escherichia coli in wastewater and river environments
Communications Biology 4(1):457.
Aquatic environments are key niches for the emergence, evolution and dissemination of antimicrobial resistance. However, the population diversity and the genetic elements that drive the dynamics of resistant bacteria in different aquatic environments are still largely unknown. The aim of this study was to understand the population genomics and evolutionary events of Escherichia coli resistant to clinically important antibiotics including aminoglycosides, in anthropogenic and natural water ecosystems. Here we show that less different E. coli sequence types (STs) are identified in wastewater than in rivers, albeit more resistant to antibiotics, and with significantly more plasmids/cell (6.36 vs 3.72). However, the genomic diversity within E. … Leer más
Delgado-Blas JF., Ovejero CM., David S., Montero N., Calero-Caceres W., Garcillan-Barcia MP., de la Cruz F., Muniesa M., Aanensen DM. y Gonzalez-Zorn B..
Description and implementation of an On-farm Wildlife Risk Mitigation Protocol at the wildlife-livestock interface: tuberculosis in Mediterranean environments
Preventive Veterinary Medicine 191:105346.
Animal tuberculosis (TB), which is caused by the Mycobacterium tuberculosis complex (MTC), is a zoonotic disease of global concern, and has a wide variety of wild and domestic reservoirs that can establish complex epidemiological systems. Of all the strategies employed to control TB, reducing the risks of interaction at the wildlife-livestock interface is a cornerstone. However, detailed protocols with which to assess and implement farm-specific preventive actions that can be employed against interactions with wildlife are lacking for extensive production systems. We describe an On-farm Wildlife Risk Mitigation Protocol that is applicable to beef cattle farming in Mediterranean environments in order to control the wildlife-livestock interac… Leer más
Martinez-Guijosa J., Lima-Barbero JF., Acevedo P., Cano-Terriza D., Jiménez-Ruiz S., Barasona JA., Boadella M., Garcia-Bocanegra I., Gortazar C. y Vicente J.
