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Últimas publicaciones científicas ISI

28 de febrero de 2018

Phylodynamics and evolutionary epidemiology of African swine fever p72-CVR genes in Eurasia and Africa

PLoS ONE 13(2):e0192565.

     African swine fever (ASF) is a complex infectious disease of swine that constitutes devastating impacts on animal health and the world economy. Here, we investigated the evolutionary epidemiology of ASF virus (ASFV) in Eurasia and Africa using the concatenated gene sequences of the viral protein 72 and the central variable region of isolates collected between 1960 and 2015. We used Bayesian phylodynamic models to reconstruct the evolutionary history of the virus, to identify virus population demographics and to quantify dispersal patterns between host species. Results suggest that ASFV exhibited a significantly high evolutionary rate and population growth through time since its divergence in the 18th century from East Africa, with no signs … Leer más

Alkhamis MA., Gallardo C., Jurado C., Soler A., Arias M. y Sanchez-Vizcaino JM..

5 de febrero de 2018

In vivo transfer and microevolution of avian native IncA/C2blaNDM-1-carrying plasmid pRH-1238 during a broiler chicken infection study

Antimicrobial Agents And Chemotherapy AAC.02128-17.

     The emergence and spread of carbapenemase-producing Enterobacteriaceae (CPE) in wildlife and livestock animals poses an important safety concern for public health. With our in vivo broiler chicken infection study we investigated transfer and experimental microevolution of the blaNDM-1-carrying IncA/C2 plasmid (pRH-1238) introduced by avian native Salmonella (S.) Corvallis, without inducing antibiotic selection pressure. We evaluated dependency of the time point of inoculation on donor [S Corvallis (12-SA01738)] and a plasmid-free Salmonella spp. recipient [S Paratyphi B (dTa+), 13-SA01617] excretion by quantifying their excretion dynamics. Using S1-PFGE plasmid profiling we gained insight into the variability of native plasmid content among… Leer más

Hadziabdic S., Fischer J., Malorny B., Borowiak M., Guerra B., Kaesbohrer A., Gonzalez-Zorn B. y Szabo I.

1 de febrero de 2018

Understanding Q Fever Risk to Humans in Minnesota Through the Analysis of Spatiotemporal Trends

Vector Borne And Zoonotic Diseases 18(2):89-95.

     Q fever is a widely distributed, yet, neglected zoonotic disease, for which domestic ruminants are considered the main reservoirs in some countries. There are still many gaps in our knowledge of its epidemiology, and the source of sporadic cases is often not determined. In this study, we show how Q fever surveillance data in combination with information routinely collected by government agencies in Minnesota during 1997 to 2015 can be used to characterize patterns of occurrence of Q fever illnesses and detect variables potentially associated with increased human illness. Cluster analysis and Bayesian spatial regression modeling revealed the presence of areas in Southern Minnesota at higher risk of Q fever. The number of sheep flocks at the … Leer más

Alvarez J., Whitten T., Branscum AJ., Garcia-Seco T., Bender JB., Scheftel J. y Perez A.

25 de enero de 2018

Emergence of chromosome borne colistin resistance gene mcr-1 in clinical isolates of Klebsiella pneumoniae from India

Antimicrobial Agents And Chemotherapy 62(2). pii: e01885-17.

     Transmissible colistin resistance gene, mcr-1 has breached the one of the last line of defense for treatment of infections caused by carbapenem-resistant Enterobacteriaceae… Leer más

Singh S., Pathak A., Kumar A., Rahman M., Singh A., Gonzalez-Zorn B. y Prasad KN.

15 de enero de 2018

In-depth resistome analysis by targeted metagenomics

Microbiome 6(1):11.

Antimicrobial resistance is a major global health challenge. Metagenomics allows analyzing the presence and dynamics of "resistomes" (the ensemble of genes encoding antimicrobial resistance in a given microbiome) in disparate microbial ecosystems. However, the low sensitivity and specificity of available metagenomic methods preclude the detection of minority populations (often present below their detection threshold) and/or the identification of allelic variants that differ in the resulting phenotype. Here, we describe a novel strategy that combines targeted metagenomics using last generation in-solution capture platforms, with novel bioinformatics tools to establish a standardized framework that allows both quantitative … Leer más

Lanza VF., Baquero F., Martinez JL., Ramos-Ruiz R., Gonzalez-Zorn B., Andremont A., Sanchez-Valenzuela A., Ehrlich SD., Kennedy S., Ruppe E., van Schaik W., Willems RJ., de la Cruz F. y Coque TM.

9 de enero de 2018

Genomic comparisons of Streptococcus suis serotype 9 strains recovered from diseased pigs in Spain and Canada

Veterinary Research 49(1):1.

     Streptococcus suis is one of the most important bacterial pathogens in the porcine industry and also a zoonotic agent. Serotype 9 is becoming one of the most prevalent serotypes within the S. suis population in certain European countries. In the present study, serotype 9 strains isolated from a country where infection due to this serotype is endemic (Spain), were compared to those recovered from Canada, where this serotype is rarely isolated from diseased pigs. For comparison purposes, strains from Brazil and the only strain isolated from a human case, in Thailand, were also incorporated. Firstly, sequence types (STs) were obtained followed by detection of putative virulence factors. Phylogenetic trees were constructed using the non-recombi… Leer más

Zheng H., Du P., Qiu X., Kerdsin A., Roy A., Bai X., Xu J., Vela AI. y Gottschalk M.

1 de enero de 2018

The relevance of host overcrowding in wildlife epidemiology: A new spatially explicit aggregation index

Ecological Indicators 84(2018)695-700.

     Species distribution and population abundance are keystone patterns in ecology, and currently also in epidemiology. The aggregation of individuals in the population is closely related to distribution and abundance, but they are not totally equivalent patterns. Despite the great efforts made in recent decades to harmonise the sampling protocols used to collect distribution and abundance data, studies regarding the development and testing of aggregation indices are scarce, even when individuals’ aggregation is quite relevant and necessary for the design of effective wildlife management policies. One of the most popular aggregation indices is the overcrowding index (m*), which quantifies the number of individuals per group, and does not, there… Leer más

Laguna E., Barasona JA., Triguero-Ocana R., Mulero-Pazmany M., Negro JJ., Vicente J. y Acevedo P.

1 de enero de 2018

Recoding of synonymous genes to expand evolutionary landscapes requires control of secondary structure affecting translation

Biotechnology And Bioengineering 115(1):184-191.

     Synthetic DNA design needs to harness the many information layers embedded in a DNA string. We previously developed the Evolutionary Landscape Painter (ELP), an algorithm that exploits the degeneracy of the code to increase protein evolvability. Here, we have used ELP to recode the integron integrase gene (intI1) in two alternative alleles. Although synonymous, both alleles yielded less IntI1 protein and were less active in recombination assays than intI1. We spliced the three alleles and mapped the activity decrease to the beginning of alternative sequences. Mfold predicted the presence of more stable secondary structures in the alternative genes. Using synonymous mutations, we decreased their stability and recovered full activity. Followi… Leer más

Escudero JA., Nivina A., Cambray G., Lopez-Igual R., Loot C. y Mazel D.

16 de diciembre de 2017

Genetic diversity assessment of Tunisian Mycobacterium bovis population isolated from cattle

BMC Veterinary Research (2017)13:393.

The genetic diversity of M. bovis in Tunisia is still underestimated despite the implementation of an eradication program. The lack of data about spatial distribution of the M. bovis population hinders the control of bovine tuberculosis (bTB) progress. This study represents the largest molecular analysis of M. bovis isolates in Tunisia. It is aimed to upgrade the understanding of bTB epidemiology and the geographical distribution of the infection. Tuberculosis research was performed in cattle (n = 149) with TB-compatible lesions collected over 5 months from a slaughterhouse located in Sfax, Tunisia.
Ninety-four animals were found to be infected by M. bovis and two others by M. caprae. Spol… Leer más

Djemal Saifeddine., Siala M., Smaoui S., Kammoun S., Marouane C., Bezos J., Messadi-Akrout F., Romero B. y Gdoura R.

1 de diciembre de 2017

Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain

Emerging Infectious Diseases 23(12):2013-2016.

     Streptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associated with different clinical backgrounds. We also investigated erythromycin resistance mechanisms and phenotypes and virulence genes. We observed 2 emm types: emm12 (11 isolates) and emm77 (4 isolates). Similarly, we observed 2 genetic linages, sequence type (ST) 26 and ST63. Most isolates exhibited the M macrolide resistance phenotype and the mefA/ermB genotype. Isolates were grouped into 2 clones on the basis of emm-MLST-PFGE-virulence gene pro… Leer más

Vela AI., Villalon P., Saez-Nieto JA., Chacon G., Dominguez L. y Fernandez-Garayzabal JF..

Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ICM 188 ZTA 78 ZTA 13
2 ZTA 183 SUAT 73 ICM 11
3 MYC 160 MYC 62 MYC 11
4 SUAT 147 ICM 42 SUAT 9
5 NED 65 NED 14 NED 3
  VISAVET 739 279 49

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.478 ZTA 3.584 ZTA 4.071
2 MYC 2.606 ICM 2.519 SEVISEQ 3.585
3 SUAT 2.452 SUAT 2.447 SUAT 3.115
4 ICM 2.286 SEVISEQ 2.251 ICM 2.749
5 SEVISEQ 2.251 SAP 2.236 MYC 2.391
VISAVET 2.630 2.671 3.069

ISI Scientific Publications
Total Last 60 mo.
1 Vet. Microbiol. 59 Transbound. Emerg. Dis. 24
2 J. Clin. Microbiol. 38 Vet. Microbiol. 21
3 Transbound. Emerg. Dis. 36 Res. Vet. Sci. 20
4 Int. J. Syst. Evol. Microbiol. 32 PLoS ONE 17
5 Res. Vet. Sci. 30 BMC Vet. Res 15
  All journals 208   All journals 32