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Últimas publicaciones científicas ISI

1 de mayo de 2018

Multicopy plasmids allow bacteria to escape from fitness trade-offs during evolutionary innovation

Nature Ecology & Evolution 2(5):873-881.

     Understanding the mechanisms governing innovation is a central element of evolutionary theory. Novel traits usually arise through mutations in existing genes, but trade-offs between new and ancestral protein functions are pervasive and constrain the evolution of innovation. Classical models posit that evolutionary innovation circumvents the constraints imposed by trade-offs through genetic amplifications, which provide functional redundancy. Bacterial multicopy plasmids provide a paradigmatic example of genetic amplification, yet their role in evolutionary innovation remains largely unexplored. Here, we reconstructed the evolution of a new trait encoded in a multicopy plasmid using TEM-1 β-lactamase as a model system. Through a combina… Leer más

Rodriguez Beltran J., Hernandez Beltran JCR., de la Fuente J., Escudero JA., Fuentes Hernandez A., Craig MacLean R., Pena Miller R. y San Millan A.

1 de mayo de 2018

The use of serological tests in combination with the intradermal tuberculin test maximizes the detection of tuberculosis infected goats

Veterinary Immunology And Immunopathology 199(2018):43-52.

     The diagnosis of tuberculosis (TB) in goats is based mainly on the single and comparative intradermal tuberculin (SIT and CIT) tests and, exceptionally, on the interferon-gamma (IFN-γ) assay, however they are not perfect in terms of sensitivity and specificity. Nevertheless, various serological assays that provide a potential cost-effective approach for the control of TB are also available or under development, and a variety of results have been reported regarding the ability of these tests to detect infected animals, particularly in the early stages of infection.
In the present study, SIT/CIT and IFN-γ tests and three different serological assays were evaluated during two consecutive herd testing events in a recently infec… Leer más

Bezos J., Roy A., Infantes-Lorenzo JA., Gonzalez I., Venteo A., Romero B., Grau A., Minguez O., Dominguez L. y de Juan L..

1 de mayo de 2018

Streptococcus penaeicida sp. nov., isolated from a diseased farmed Pacific white shrimp (Penaeus vannamei)

International Journal Of Systematic And Evolutionary Microbiology 68(5):1490-1495.

     Strain CAIM 1838T, isolated from the hepatopancreas of a cultured diseased Pacific white shrimp (Penaeusvannamei), was subjected to characterization by a polyphasic taxonomic approach. On the basis of 16S rRNA gene sequence analysis, strain CAIM 1838T was most closely related to Streptococcus bovimastitidis 99.3 % and to other species of the Pyogenes clade of Streptococcus with lower similarity values. Average nucleotide identity values and the genome-to-genome distance of strain CAIM 1838T, as compared with the type strains, confirmed the separate species status with closely related species of the genus Streptococcus and were all below the thresholds to delimit a species, 93.1 and 49.4 %, respectively. The DNA G+C content… Leer más

Morales-Covarrubias MS., Del Carmen Bolan-Mejía M., Vela AI., Fernandez-Garayzabal JF. y Gomez-Gil B.

9 de abril de 2018

Ovine Mannheimia haemolytica isolates from lungs with and without pneumonic lesions belong to similar genotypes

Veterinary Microbiology 2019(2018)80-86.

     This study investigated the genetic characteristics of 121 ovine Mannheimia haemolytica isolates from lungs with (n = 75) and without pneumonic lesions (n = 46) using multilocus sequence typing (MLST), virulence-associated gene typing and pulsed-field gel electrophoresis (PFGE). Twelve STs were identified with most isolates (81%) belonged to ST16, ST28 and ST8. Analysis of the M. haemolytica MLST Database indicate a wide distribution of these genotypes in small ruminants, never reported in bovine isolates. This could suggest the adaptation of certain genetic lineages of M. haemolytica to small ruminants. e-BURST analysis grouped most STs into three clonal complexes (CC2, CC8 and CC28), consistent with a clonal popula… Leer más

Garcia-Alvarez A., Fernandez-Garayzabal JF., Chaves F., Pinto C. y Cid D.

1 de abril de 2018

Relevant Measures to Prevent the Spread of African Swine Fever in the European Union Domestic Pig Sector

Frontiers In Veterinary Science 16;5:77.

     During the past decade, African swine fever (ASF) has spread from the Caucasus region to eastern European Union countries affecting domestic pig and wild boar populations. In order to avert ASF spread, mitigation measures targeting both populations have been established. However, despite these efforts, ASF has been reported in thirteen different countries (Georgia, Azerbaijan, Armenia, the Russian Federation, Ukraine, Belarus, Estonia, Latvia, Lithuania, Poland, Moldova, Czech Republic, and Romania). In the absence of an effective vaccine or treatment to ASF, introduction and spread of ASF onto domestic pig farms can only be prevented by strict compliance to control measures. This study systematically reviewed available measures to prevent … Leer más

Jurado C., Martinez-Aviles M., de la Torre A., Stukelj M., Cardoso de Carvalho Ferreira H., Cerioli M., Sanchez-Vizcaino JM. y Bellini S.

1 de abril de 2018

Why is African swine fever still present in Sardinia?

Transboundary And Emerging Diseases 65(2):557-566.

     African swine fever (ASF) is an infectious disease of swine that has been present in Sardinia since 1978. Soon after introduction of the disease, several control and eradication programmes were established with limited success. Some researchers attributed the persistence of the disease in central and eastern areas to certain socio-economic factors, the existence of some local and traditional farming practices (i.e., unregistered free-ranging pigs known as brado animals) and the high density of wild boar in the region. In the past, scarcity of swine data in Sardinia complicated the evaluation and study of ASF on the island. More complete, accurate and reliable information on pig farms has become available as a result of the most recent eradi… Leer más

Jurado C., Fernandez-Carrion E., Mur L., Rolesu S., Laddomada A. y Sanchez-Vizcaino JM..

22 de marzo de 2018

An advection-deposition-survival model to assess the risk of introduction of vector-borne diseases through the wind: Application to bluetongue outbreaks in Spain

PLoS ONE 13(3):e0194573.

     This work develops a methodology for estimating risk of wind-borne introduction of flying insects into a country, identifying areas and periods of high risk of vector-borne diseases incursion. This risk can be characterized by the role of suitable temperatures and wind currents in small insects` survival and movements, respectively. The model predicts the number density of introduced insects over space and time based on three processes: the advection due to wind currents, the deposition on the ground and the survival due to climatic conditions. Spanish livestock has suffered many bluetongue outbreaks since 2004 and numerous experts point to Culicoides transported by wind from affected areas in North Africa as a possible cause. This work imp… Leer más

Fernandez-Carrion E., Ivorra B., Ramos AM., Martinez-Lopez B., Aguilar-Vega C. y Sanchez-Vizcaino JM..

16 de marzo de 2018

PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samples Reveals Their Importance as Gene Capture Platforms

Frontiers In Microbiology 9(469):1-15.

     ColE1 plasmids are important vehicles for the spread of antibiotic resistance in the Enterobacteriaceae and Pasteurellaceae families of bacteria. Their monitoring is essential, as they harbor important resistant determinants in humans, animals and the environment. In this work, we have analyzed ColE1 replicons using bioinformatic and experimental approaches. First, we carried out a computational study examining the structure of different ColE1 plasmids deposited in databases. Bioinformatic analysis of these ColE1 replicons revealed a mosaic genetic structure consisting of a host-adapted conserved region responsible for the housekeeping functions of the plasmid, and a variable region encoding a wide variety of genes, including multiple antib… Leer más

Ares M., Bernabe-Balas C., Santos-Lopez A., Baquero MR., Prasad KN., Cid D., Martin-Espada C., San Millan A. y Gonzalez-Zorn B..

28 de febrero de 2018

Phylodynamics and evolutionary epidemiology of African swine fever p72-CVR genes in Eurasia and Africa

PLoS ONE 13(2):e0192565.

     African swine fever (ASF) is a complex infectious disease of swine that constitutes devastating impacts on animal health and the world economy. Here, we investigated the evolutionary epidemiology of ASF virus (ASFV) in Eurasia and Africa using the concatenated gene sequences of the viral protein 72 and the central variable region of isolates collected between 1960 and 2015. We used Bayesian phylodynamic models to reconstruct the evolutionary history of the virus, to identify virus population demographics and to quantify dispersal patterns between host species. Results suggest that ASFV exhibited a significantly high evolutionary rate and population growth through time since its divergence in the 18th century from East Africa, with no signs … Leer más

Alkhamis MA., Gallardo C., Jurado C., Soler A., Arias M. y Sanchez-Vizcaino JM..

5 de febrero de 2018

In vivo transfer and microevolution of avian native IncA/C2blaNDM-1-carrying plasmid pRH-1238 during a broiler chicken infection study

Antimicrobial Agents And Chemotherapy AAC.02128-17.

     The emergence and spread of carbapenemase-producing Enterobacteriaceae (CPE) in wildlife and livestock animals poses an important safety concern for public health. With our in vivo broiler chicken infection study we investigated transfer and experimental microevolution of the blaNDM-1-carrying IncA/C2 plasmid (pRH-1238) introduced by avian native Salmonella (S.) Corvallis, without inducing antibiotic selection pressure. We evaluated dependency of the time point of inoculation on donor [S Corvallis (12-SA01738)] and a plasmid-free Salmonella spp. recipient [S Paratyphi B (dTa+), 13-SA01617] excretion by quantifying their excretion dynamics. Using S1-PFGE plasmid profiling we gained insight into the variability of native plasmid content among… Leer más

Hadziabdic S., Fischer J., Malorny B., Borowiak M., Guerra B., Kaesbohrer A., Gonzalez-Zorn B. y Szabo I.

Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ICM 189 ZTA 80 ZTA 15
2 ZTA 186 SUAT 73 ICM 12
3 MYC 162 MYC 62 SUAT 10
4 SUAT 150 ICM 43 MYC 9
5 NED 65 NED 14 NED 3
  VISAVET 748 281 50

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.481 ZTA 3.598 ZTA 3.923
2 MYC 2.597 ICM 2.494 SEVISEQ 3.585
3 SUAT 2.435 SUAT 2.423 SUAT 2.795
4 ICM 2.288 SEVISEQ 2.251 ICM 2.698
5 SAP 2.236 SAP 2.236 MYC 2.191
VISAVET 2.625 2.665 2.946

ISI Scientific Publications
Total Last 60 mo.
1 Vet. Microbiol. 60 Transbound. Emerg. Dis. 25
2 J. Clin. Microbiol. 38 Vet. Microbiol. 21
3 Transbound. Emerg. Dis. 36 Res. Vet. Sci. 19
4 Int. J. Syst. Evol. Microbiol. 32 PLoS ONE 17
5 Res. Vet. Sci. 30 BMC Vet. Res 14
  All journals 209   All journals 30