Últimas publicaciones científicas SCI
Minigene Splicing Assays Identify 12 Spliceogenic Variants of BRCA2 Exons 14 and 15
Frontiers In Genetics 10:503.
A relevant fraction of BRCA2 variants is associated with splicing alterations and with an increased risk of hereditary breast and ovarian cancer (HBOC). In this work, we have carried out a thorough study of variants from BRCA2 exons 14 and 15 reported at mutation databases. A total of 294 variants from exons 14 and 15 and flanking intronic sequences were analyzed with the online splicing tools NNSplice and Human Splicing Finder. Fifty-three out of these 294 variants were selected as candidate splicing variants. All variants but one, were introduced into the minigene MGBR2_ex14-20 (with exons 14-20) by site-directed mutagenesis and assayed in MCF-7 cells. Twelve of the remaining 52 variants (23.1%) impaired splicing at different degrees, yie… Leer más
Fraile-Bethencourt E., Valenzuela-Palomo A., Diez-Gomez B., Caloca MJ., Gomez S. y Velasco EA.
Comparison of spatiotemporal patterns of historic natural Anthrax outbreaks in Minnesota and Kazakhstan
PLoS ONE 14(5):e0217144.
Disease spread in populations is a consequence of the interaction between host, pathogen, and environment, i.e. the epidemiological triad. Yet the influences of each triad component may vary dramatically for different settings. Comparison of environmental, demographic, socio-economic, and historical backgrounds may support tailoring site-specific control measures. Because of the long-term survival of Bacillus anthracis, Anthrax is a suitable example for studying the influence of triad components in different endemic settings. We compared the spatiotemporal patterns of historic animal Anthrax records in two endemic areas, located at northern latitudes in the western and eastern hemispheres. Our goal was to compare the spatiotemporal patterns… Leer más
Kanankege KST., Abdrakhmanov SK., Alvarez J., Glaser L., Bender JB., Mukhanbetkaliyev YY., Korennoy FI., Kadyrov AS., Abdrakhmanova AS. y Perez AM.
Development and characterization of mouse monoclonal antibodies to eight human complement components: analysis of reactivity with orthologs of nine mammalian genera
Comparative Immunology, Microbiology And Infectious Diseases 62:7-12.
Abstract
To study complement function in mammalian leishmanioses, we developed mouse monoclonal antibodies to the human complement system components C1q, C4, factor D, factor H, factor B, properdin, C5 and C9. Antibody specificity was determined by indirect and capture ELISA and by Western blot. In flow cytometry analysis, seven antibodies recognized the cognate component on human serum-opsonized Leishmania promastigotes. Antibody reactivity was screened against promastigotes opsonized with sera of nine mammalian genera: pig, guinea pig, goat, rabbit, cat, dog, hamster, jird and rat. No antibody recognized jird epitopes on promastigotes. Anti-C4, -properdin, and -C5b reacted with the orthologous protein of all other mammals tested ex… Leer más
Monedero M., Infantes-Lorenzo JA., Moreno I., Martin AB., Macias RIR., Torano A. y Dominguez M.
Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas
Veterinary Research 50(1):32.
Lactococcus garvieae is a significant pathogen in aquaculture with a potential zoonotic risk. To begin to characterize the late immune response of trout to lactococcosis, we selected infected individuals showing clinical signs of lactococcosis. At the time lactococcosis clinical signs appeared, infection by L. garvieae induced a robust inflammatory response in the spleen of rainbow trout, which correlated with abundant granulomatous lesions. The response in kidney goes in parallel with that of spleen, and most of the gene regulations are similar in both organs. A correlation existed between the early inflammatory granulomas in spleen (containing macrophages with internalized L. garvieae) and up-regulated gene sets, which defined the presenc… Leer más
Castro R., Coll J., Blanco MDM., Rodriguez-Bertos A., Jouneau L., Fernandez-Garayzabal JF. y Gibello A.
Antimicrobial susceptibility and genetic characterization of Trueperella pyogenes isolates from pigs reared under intensive and extensive farming practices
Veterinary Microbiology 232:89-95.
Trueperella pyogenes is an opportunistic pathogen associated with a variety of diseases and responsible for important economic losses for pig production. Minimal Inhibitory Concentration (MIC) and Pulsed Field Gel Electrophoresis (PFGE) typing analysis were used to determine the MIC distribution and to genetically characterize a total of 180 T. pyogenes isolates obtained from slaughtered pigs reared under intensive (TpIN, n = 89) and extensive (TpEX, n = 91) farming practices. Low MIC90 values for penicillin and amoxicillin (0.008 and 0.06 μg/ml, respectively), ceftiofur, gentamicin and enrofloxacin (1 μg/ml, respectively) were obtained, so they could be of choice for the empiric treatment of T.… Leer más
Galan-Relaño A., Gomez-Gascon L., Luque I., Barrero-Dominguez B., Casamayor A., Cardoso-Toset F., Vela AI., Fernandez-Garayzabal JF. y Tarradas C.
Seroprevalence of Brucella canis antibodies in dogs entering a Minnesota humane society, Minnesota, 2016-2017
Preventive Veterinary Medicine 168:90-94.
Background: Canine brucellosis, caused by the bacterium Brucella canis, is a zoonotic and largely reproductive disease of dogs. The disease is a recognized problem in canine breeding populations, and the risk to individuals assisting with birthing is well described. Prior to 2015, all cases of canine brucellosis reported to the Minnesota Board of Animal Health were in dogs used for breeding. In 2015, canine brucellosis was identified in eight Minnesota rescue dogs, all originating from specific geographic areas in South Dakota. Our objective was to measure the seroprevalence of B. canis in stray and previously owned dogs entering a large Minnesota animal rescue organization to determine if our observations represented a localized or general… Leer más
Whitten T., Brayshaw G., Patnayak D., Alvarez J., Larson CM., Root-Kustritz M., Holzbauer SM., Torrison J. y Scheftel J.
First Oral Vaccination of Eurasian Wild Boar Against African Swine Fever Virus Genotype II
Frontiers In Veterinary Science 6(137):1-10.
African swine fever (ASF), the most significant threat to the pig industry worldwide, has spread to more than 55 countries on three continents, and it affects more than 77% of the world swine population. In the European Union, wild boar (Sus scrofa) is the most severely affected host. The main reasons for the unprecedented and constant spread of ASF in Europe are the trade activities, the continuous movement of infected-wild boar populations among regions and the lack of vaccine to prevent ASF infection. In this study, we demonstrate that oral immunization of wild boar with a non-hemadsorbing, attenuated ASF virus of genotype II isolated in Latvia in 2017 (Lv17/WB/Rie1) conferred 92% protection against challenge with a virulent ASF virus is… Leer más
Barasona JA., Gallardo C., Cadenas-Fernández E., Jurado C., Rivera B., Rodriguez-Bertos A., Arias M. y Sanchez-Vizcaino JM..
Pasteurella multocida isolates associated with ovine pneumonia are toxigenic
Veterinary Microbiology 232:40_73.
The P. multocida toxin (PMT), a dermonecrotic protein encoded by the toxA gene, is the major virulence factor of capsular type D P. multocida strains causing progressive atrophic rhinitis (PAR) in pigs. A high frequency of P. multocida isolates harboring the toxA gene has been found among ovine pneumonic isolates, although the ability of these isolates to express PMT has never been examined. In this study we have investigated the ability of ovine toxA+ P. multocida isolates (n = 57) to express a functional toxin by detection of PMT toxin antigen using an ELISA test and its cytopathic effect in a Vero cell assay. PMT antigen was expressed in the great majority (54/57; 94.7%) of toxA+ isolates. Moreover, the 100% toxA+ ovine isola… Leer más
Cid D., Garcia-Alvarez A., Dominguez L., Fernandez-Garayzabal JF. y Vela AI..
National colistin sales versus colistin resistance in Spanish pig production
Research In Veterinary Science 123:141-143.
Colistin resistance was associated with infrequent spontaneous mutations that reduce bacterial fitness until 2015, when the plasmid-mediated colistin resistance gene mcr-1 was discovered in China (Liu et al., 2016). The mcr-1 gene was reported for the first time in Southern Europe in 2016 in nine strains of Escherichia coli and Salmonella enterica from farm animals (poultry and pigs) (Quesada et al., 2016). Currently, eight different mcr genes have been reported (mcr-1 to 8) (Garcia et al., 2018) (Yang et al., 2018) (Wang et al., 2018). Nevertheless, only mcr-1, 3, and 4 have been found in Spain (Hernandez et al., 2017) (Quesada et al., 2016) (Carattoli et al., 2017).
Food-producing animals, particularly pigs, have been shown… Leer más
Miguela-Villoldo P., Hernandez M., Moreno MA., Rodriguez-Lazaro D., Quesada A., Dominguez L. y Ugarte-Ruiz M..
Circulation of plasmids harboring resistance genes to quinolones and/or extended-spectrum cephalosporins in multiple Salmonella enterica serotypes from swine in the United States
Antimicrobial Agents And Chemotherapy 63(4):e02602-18 .
Nontyphoidal Salmonella enterica (NTS) poses a major public-health risk worldwide that is amplified by the existence of antimicrobial resistant strains, especially to quinolones and extended-spectrum cephalosporins (ESC). Little is known on the dissemination of plasmids harboring the acquired genetic determinants that confer resistance to these antimicrobials across NTS serotypes from livestock in the United States.NTS isolates (n=183) from U.S. swine clinical cases retrieved during 2014-2016 were selected for sequencing based on their phenotypic resistance to enrofloxacin (quinolone) or ceftiofur (3rd-generation cephalosporin). De-novo assemblies were used to identify chromosomal mutations and acquired antimicrobial resistance genes (AARGs… Leer más
Elnekave E., Hong SL., Lim S., Hayer SS., Boxrud D., Taylor AJ., Lappi V., Noves N., Johnson TJ., Rovira A., Davies P., Perez A. y Alvarez J..
