Últimas publicaciones científicas SCI
Effect of heat-inactivated Mycobacterium avium subspecies paratuberculosis (MAP) vaccine on the lesions and immunopathology developed in target tissues of naturally MAP-infected goats
Veterinary Microbiology 273:109543.
Paratuberculosis (PTB) is a disease caused by Mycobacterium avium subspecies paratuberculosis (MAP), which affects a broad range of hosts, including domestic and wild animals. PTB is a chronic granulomatous enteritis and lymphadenitis that compromises animal welfare and causes economic losses. The objective of this study was to evaluate the effect of a commercial heat-inactivated MAP vaccine on lesions and immunopathology developed in the target tissues of goats naturally infected with MAP. Lesions compatible with PTB in the intestine and regional lymph nodes (LNs), as well as local immune response to MAP, were evaluated and compared in Gudair®-vaccinated (n = 14) and unvaccinated (n = 11) goats from a MAP-infected farm. The percentage of a… Leer más
Agullo-Ros I., Andrada M., Perez-Sancho M., Roy A., Bezos J., Bonnet T., Moreno I., Paz-Sanchez Y., Dominguez M., Gomez-Villamandos JC., Dominguez L. y Risalde MA.
Patterns of antimicrobial resistance in Salmonella isolates from fattening pigs in Spain
BMC Veterinary Research 18(1):333.
Swine are considered a major source of foodborne salmonellosis, a public health issue further complicated by the circulation of multidrug-resistant Salmonella strains that threaten the safety of the food chain. The current study aimed to identify patterns that can help to understand the epidemiology of antimicrobial resistance (AMR) in Salmonella in pigs in Spain through the application of several multivariate statistical methods to data from the AMR national surveillance programs from 2001 to 2017.
A total of 1,318 pig Salmonella isolates belonging to 63 different serotypes were isolated and their AMR profiles were determined. Tetracycline resistance across provinces in Spain was the highest among all antimicrobials and rang… Leer más
Teng KT., Ugarte-Ruiz M., Moreno MA., Saez-Llorente JL., Collado S., De Frutos C., Dominguez L. y Alvarez J..
Evaluation of the clinical evolution and transmission of SARS-CoV-2 infection in cats by simulating natural routes of infection
Veterinary Research Communications 46(3):837-852.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the current pandemic disease denominated as Coronavirus Disease 2019 (COVID-19). Several studies suggest that the original source of this virus was a spillover from an animal reservoir and its subsequent adaptation to humans. Of all the different animals affected, cats are one of the most susceptible species. Moreover, several cases of natural infection in domestic and stray cats have been reported in the last few months. Although experimental infection assays have demonstrated that cats are successfully infected and can transmit the virus to other cats by aerosol, the conditions used for these experiments have not been specified in terms of ventilation. … Leer más
Barroso-Arevalo S., Sánchez-Morales L., Barasona JA., Rivera B., Sanchez R., Risalde MA., Argullo-Ros I. y Sanchez-Vizcaino JM..
Harmonisation of in-silico next-generation sequencing based methods for diagnostics and surveillance
Scientific Reports 12(1):14372.
Improvements in cost and speed of next generation sequencing (NGS) have provided a new pathway for delivering disease diagnosis, molecular typing, and detection of antimicrobial resistance (AMR). Numerous published methods and protocols exist, but a lack of harmonisation has hampered meaningful comparisons between results produced by different methods/protocols vital for global genomic diagnostics and surveillance. As an exemplar, this study evaluated the sensitivity and specificity of five well-established in-silico AMR detection software where the genotype results produced from running a panel of 436 Escherichia coli were compared to their AMR phenotypes, with the latter used as gold-standard. The pipelines exploited previously known geno… Leer más
Nunez-Garcia J., Abuoun M., Storey N., Brouwer MS., Delgado-Blas JF., Mo SS., Ellaby N., Veldman KT., Haenni M., Chatre P., Madec JY., Hammerl JA., Serna-Bernaldo C., Getino M., La Ragione R., Naas T., Telke AA., Glaser A., Sunde M., Gonzalez-Zorn B., Ellington MJ. y Anjum MF.
Hidden dissemination of carbapenem-susceptible OXA-48-producing Proteus mirabilis
The Journal Of Antimicrobial Chemotherapy dkac267.
Objectives: To detect a potential hidden dissemination of the blaOXA-48 gene among Proteus mirabilis isolates obtained from a single centre.
Methods: P. mirabilis from diverse clinical samples presenting an ESBL phenotype or obtained from blood cultured from 2017 to 2019 were evaluated. Bacterial identification was performed using MALDI-TOF MS. MICs were determined using International Organization for Standardization (ISO) standard microdilution and interpreted following EUCAST guidelines. WGS was performed using both short- and long-read technologies and assemblies were done using Unicycler. Resistomes were assessed using the ResFinder database. SNPs were detected using the PATRIC bioinformatics platform. Cloning experiments were pe… Leer más
Pedraza R., Kieffer N., Guzman-Puche J., Artacho MJ., Pitart C., Hernandez-Garcia M., Vila J., Canton R. y Martinez-Martinez L.
The Omicron (B.1.1.529) SARS-CoV-2 variant of concern also affects companion animals
Frontiers In Veterinary Science 9:940710.
The emergence of the Omicron variant (B.1. 1.529) has brought with it an increase in the incidence of SARS-CoV-2 disease. However, there is hardly any data on its incidence in companion animals. We have detected the presence of this new variant in domestic animals (dogs and cats) living with infected owners in Spain. None of the RT-qPCR positive animals (10.13%) presented any clinical signs and the viral loads detected were low. In addition, the shedding of viral RNA lasted a short period of time in the positive animals. Infection with this variant of concern (VOC) was confirmed by RT-qPCR and sequencing. These outcomes suggest a lower virulence of this variant in infected cats and dogs. They also demonstrate the transmission from infected … Leer más
Sánchez-Morales L., Sanchez-Vizcaino JM., Perez-Sancho M., Dominguez L. y Barroso P.
The expression of aminoglycoside resistance genes in integron cassettes is not controlled by riboswitches
Nucleic Acids Research 50(15):8566-8579.
Regulation of gene expression is a key factor influencing the success of antimicrobial resistance determinants. A variety of determinants conferring resistance against aminoglycosides (Ag) are commonly found in clinically relevant bacteria, but whether their expression is regulated or not is controversial. The expression of several Ag resistance genes has been reported to be controlled by a riboswitch mechanism encoded in a conserved sequence. Yet this sequence corresponds to the integration site of an integron, a genetic platform that recruits genes of different functions, making the presence of such a riboswitch counterintuitive. We provide, for the first time, experimental evidence against the existence of such Ag-sensing riboswitch. We … Leer más
Hipolito A., Garcia-Pastor L., Blanco P., Trigo de Roza F., Kieffer N., Vergara E., Jove T., Alvarez J. y Escudero JA..
Budd-Chiari-like pathology in dolphins
Scientific Reports 12(1):12635.
Nearly two decades ago, pathologic examination results suggested that acoustic factors, such as mid-frequency active naval military sonar (MFAS) could be the cause of acute decompression-like sickness in stranded beaked whales. Acute systemic gas embolism in these whales was reported together with enigmatic cystic liver lesions (CLL), characterized by intrahepatic encapsulated gas-filled cysts, tentatively interpreted as "gas-bubble" lesions in various other cetacean species. Here we provide a pathologic reinterpretation of CLL in odontocetes. Among 1,200 cetaceans necropsied, CLL were only observed in four striped dolphins (Stenella coeruleoalba), with a low prevalence (2%, N = 179). Together, our data strongly suggest that CLL are the res… Leer más
Fernandez A., Jepson PD., Diaz-Delgado J., Bernaldo de Quiros Y., Sierra E., Mompeo B., Vela AI., Di Guardo G., Suarez-Santana C., Espinosa de los Monteros A., Herraez P., Andrada M., Caballero MJ., Rivero M., Consoli F., Castro-Alonso A., Quesada-Canales O. y Arbelo M.
Corynebacterium conjunctivae: A New Corynebacterium Species Isolated from the Ocular Surface of Healthy Horses
Animals 12(14):1827.
Twenty-two unidentified Gram-positive, rod-shaped organisms were recovered from the conjunctival surface of apparently healthy horses and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Corynebacterium, although they did not match any recognized species. Comparative 16S rRNA gene sequencing studies demonstrated that all of the isolates were phylogenetically members of the genus Corynebacterium. The isolates shared 99.4 to 100% 16S rRNA gene sequence similarity among the strains and 96.5% similarity with Corynebacterium tapiri 2385/12T, which was the closest phylogenetically related species. The DNA G+C content was 58.4 mol%. The major fa… Leer más
Fernandez-Garayzabal JF., LaFrentz S., Casamayor A., Abarca E., Mohammed HH., Cuming RS., Arias CR., Dominguez L. y Vela AI..
Use of Whole-Genome Sequencing to Unravel the Genetic Diversity of a Prevalent Mycobacterium bovis Spoligotype in a Multi-Host Scenario in Spain
Frontiers In Microbiology 13:915843.
Despite the efforts invested in the eradication of bovine tuberculosis in Spain, herd prevalence has remained constant in the country during the last 15 years (~1.5–1.9%) due to a combination of epidemiological factors impairing disease control, including between-species transmission. Here, our aim was to investigate the molecular diversity of Mycobacterium bovis isolates belonging to the highly prevalent SB0339 spoligotype in the cattle-wildlife interface in different regions of Spain using whole-genome sequencing (WGS). Genomic data of 136 M. bovis isolates recovered from different animal species (cattle, wild boar, fallow deer, and red deer) and locations between 2005 and 2018 were analyzed to investigate between- and within-specie… Leer más
Pozo P., Lorente-Leal V., Robbe-Austerman S., Hicks J., Stuber T., Bezos J., de Juan L., Saez-Llorente JL., Romero B., Alvarez J. y on behalf of the Spanish Network on Surveillance Monitoring of Animal Tuberculosis.
