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10 de abril de 2024

Time, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples

Veterinary Research Communications 1-11.

     The isolation of Campylobacter fetus subsp. venerealis (Cfv) from clinical samples is the gold standard for confirming cases of bovine genital campylobacteriosis, an important cause of infertility in cattle and a potential public health concern. Furthermore, isolation is also necessary for the development of autologous vaccines, characterization of strains for antimicrobial susceptibility patterns, etc. Nevertheless, the sensitivity of culture methods is usually low, and there is no standardized protocol to maximize the recovery of Cfv from clinical samples. The aim of the current study is to design a protocol for the culture of Cfv from preputial samples by evaluating the combination of different transport, enrichment and culture media con… Leer más

Polo C., Garcia-Seco T., Garcia N., Fernandez-Benito V., Briones V., Diez-Guerrier A., Alvarez J., Dominguez L. y Perez-Sancho M..

2 de abril de 2024

Forensic findings in urban red fox mortality in the metropolitan area of Madrid, 2014-2022

Journal Of Veterinary Diagnostic Investigation 1-5.

     The expanding presence of red foxes (Vulpes vulpes) in urban and suburban regions could potentially lead to increased instances of human aggression towards this species. We studied 10 deceased red foxes that were submitted by law enforcement agencies in the metropolitan area of Madrid in 2014-2022 because of suspected abuse. Forensic autopsies were performed to establish the cause and manner of death. In 4 of the 10 cases, the cause of death was deemed unnatural, involving blunt-force trauma (n = 2), asphyxia resulting from hanging (n = 1), and firearm injury (n = 1). Among the remaining cases, most had succumbed to natural causes (n = 4), often marked by severe emaciation and a high burden of parasites, primarily Sarcoptes scabiei. In 2 ca… Leer más

Rebollada A., Barcena C., Porras N., Dominguez L. y Rodriguez-Bertos A..

27 de marzo de 2024

Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus

The Veterinary Quarterly 44(1):1-13.

     Despite the great interest in the development of a vaccine against African swine fever (ASF) in wild boar, the immunological mechanisms that induce animal protection are still unknown. For this purpose, tertiary lymphoid organs (TLOs) of wild boar were characterised and compared with mucosa-associated lymphoid tissues (MALTs) by histopathology, histomorphometry and immunohistochemistry (CD3, CD79, PAX5, LYVE1, fibronectin). In addition, real-time polymerase chain reaction (qPCR) and immunohistochemistry (p72) were used to evaluate the presence of ASF virus (ASFV) in blood and tissues samples, respectively. TLOs were observed in animals infected with a low-virulent ASFV isolate (LVI), animals co-infected with low and high-virulent ASFV isola… Leer más

Porras N., Sanchez-Vizcaino JM., Rodriguez-Bertos A., Kosowska A. y Barasona JA..

22 de marzo de 2024

Eco-Sanitary Regionalization of Wild Boar (Sus scrofa) in the Western Palearctic Realm as a Tool for the Stewardship of African Swine Fever

Transboundary And Emerging Diseases 8080496.

     African swine fever (ASF) is a viral hemorrhagic disease affecting domestic and wild suids, with catastrophic socioeconomic impact worldwide. In the European scenario, wild boar (Sus scrofa) actively contributes to the maintenance and spread of the disease. In this study, we applied a multivariate clustering method to define ecoregions in the western Palearctic realm based on ecological and sanitary aspects of ASF in wild boar. Beforehand, we performed a principal components analysis for the eight selected variables. An analysis of the countries was carried out in terms of the extent of ecoregions and ASF notifications. After clustering, we identified nine eco-regions that showed important differences based on the used eco-sanitary variable… Leer más

Aguilar-Vega C., Munoz-Perez C., Sanchez-Vizcaino JM., Martinez-Aviles M. y Bosch J.

9 de marzo de 2024

Molecular detection of herpesvirus in a skin lesion of a humpback whale (Megaptera novaeangliae) from the Western Mediterranean Sea

European Journal Of Wildlife Research 70:31.

     Herpesvirus has the potential to infect a wide variety of animal species. In cetaceans, Alpha- and/or Gammaherpesvirinae have been identified in eight families of odontocetes, and one family of mysticetes. In May 2022, an adult humpback whale (Megaptera novaeangliae) was found stranded in Valencia, Spain. The whale was emaciated, in poor body condition, with multiple lacerations on the dorsal fin and a high number of epibionts of the Cyamidae family, known as whale lice. The individual had been previously released from a ghost net entanglement 5 days before becoming stranded. In a closer examination, various skin lesions were observed, including chronic, proliferative, and erosive dermatitis and a large ulcer extending to the deep dermis. A… Leer más

Vargas-Castro I., Crespo-Picazo JL., Jimenez-Martinez MA., Munoz-Baquero M., Marco-Cabedo V., Garcia-Parraga D. y Sanchez-Vizcaino JM..

8 de marzo de 2024

Pancreatic adenocarcinoma treated with surgical resection, toceranib phosphate and firocoxib in a dog: a case report

Veterinary Research Communications 1-7.

     Exocrine pancreatic carcinomas are rarely reported in dogs. A ductal pancreatic adenocarcinoma in a 10-year-old intact beagle is described in this report. The diagnosis was made based on clinical signs, imaging (abdominal ultrasound and CT scan) and histopathology. Treatment consisted of partial right lobe pancreatectomy followed by adjuvant therapy with toceranib phosphate (Palladia®) and firocoxib (Previcox®) for six months. The treatment was well tolerated, and the survival time was 445 days. To our knowledge, this is the longest survival reported in the literature for a dog diagnosed with exocrine pancreatic adenocarcinoma. The results described here may contribute to provide a better understanding about this neoplasia and potential tre… Leer más

Fuertes-Recuero M., Vazquez-Fernandez E., Lizasoain-Sanz G., Arroba-Alonso A., Sanchez-Lopez A., Martinez-de Merlo E., Suarez-Redondo M. y Ortiz-Diez G.

4 de marzo de 2024

Application of machine learning with large-scale data for an effective vaccination against classical swine fever for wild boar in Japan

Scientific Reports 14(1):5312.

     Classical swine fever has been spreading across the country since its re-emergence in Japan in 2018. Gifu Prefecture has been working diligently to control the disease through the oral vaccine dissemination targeting wild boars. Although vaccines were sprayed at 14,000 locations between 2019 and 2020, vaccine ingestion by wild boars was only confirmed at 30% of the locations. Here, we predicted the vaccine ingestion rate at each point by Random Forest modeling based on vaccine dissemination data and created prediction surfaces for the probability of vaccine ingestion by wild boar using spatial interpolation techniques. Consequently, the distance from the vaccination point to the water source was the most important variable, followed by elev… Leer más

Ito S., Aguilar-Vega C., Bosch J., Isoda N. y Sanchez-Vizcaino JM..

28 de febrero de 2024

Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats

Veterinary Sciences 11(3):105.

     Paratuberculosis (PTB) and tuberculosis (TB) are two mycobacterial diseases with a severe economic and health impact on domestic ruminants. The ante mortem diagnosis of PTB is hampered, among other factors, by the limited sensitivity of all the available diagnostic techniques. Since TB-infected goats subjected to the comparative intradermal tuberculin test (CITT) may experience a booster effect on their antibody titer and a potential enhancement to the sensitivity of humoral techniques for tuberculosis, in the present study we aimed to evaluate this diagnostic strategy on the humoral diagnosis of PTB in serum and milk samples collected from a caprine herd that was TB free and PTB infected. The results from 120 goats indicated a significant … Leer más

Velasco-Reinaldos C., Ortega J., Gómez-Buendía A., Grau A., Lopez M., Alvarez J., Romero B., de Juan L. y Bezos J..

16 de febrero de 2024

Phenotypic and genetic characterization of antimicrobial resistance in Salmonella enterica serovar Choleraesuis isolates from humans and animals in Spain from 2006 to 2021

The Journal Of Antimicrobial Chemotherapy 1-11.

     Objectives
While an increase in the levels of MDR in Salmonella enterica sevorar Choleraesuis has been reported in Europe, little is known about the situation in Spain. Therefore, we first aimed to assess the phenotypic resistance profile and to determine the presence of genetic determinants of resistance of S. Choleraesuis isolates collected in animal and human. Our second objective was to identify and characterize clusters of highly related isolates.

Methods
We analysed 50 human and 45 animal isolates retrieved from 2006 to 2021 using the disc diffusion method and performed WGS followed by analyses of genetic determinants and phylogenetic analysis.

Results
All isolates were of ST145 and c… Leer más

Jacqueline C., Samper-Cativiela C., Monzon S., Ugarte-Ruiz M., Cuesta I., Alvarez J. y Herrera-Leon S.

12 de febrero de 2024

Landscape connectivity for predicting the spread of ASF in the European wild boar population

Scientific Reports 10;14(1):3414.

     African swine fever (ASF) is an infectious and highly fatal disease affecting wild and domestic swine, which is unstoppably spreading worldwide. In Europe, wild boars are one of the main drivers of spread, transmission, and maintenance of the disease. Landscape connectivity studies are the main discipline to analyze wild-species dispersal networks, and it can be an essential tool to predict dispersal-wild boar movement routes and probabilities and therefore the associated potential ASF spread through the suitable habitat. We aimed to integrate wild boar habitat connectivity predictions with their occurrence, population abundance, and ASF notifications to calculate the impact (i.e., the capacity of a landscape feature to favor ASF spread) an… Leer más

Goicolea T., Cisneros-Araujo P., Aguilar-Vega C., Sanchez-Vizcaino JM., Mateo-Sanchez MC. y Bosch J.





Science Publication
Indicators
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ZTA 248 SUAT 64 SUAT 16
2 ICM 236 ZTA 52 MYC 12
3 MYC 227 MYC 49 SAP 11
4 SUAT 219 ICM 42 ZTA 5
5 NED 75 SAP 31 ICM 5
  VISAVET 1120 322 57

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 SAP 3.898 ZTA 5.366 ZTA 6.42
2 ZTA 3.875 SUAT 4.187 SAP 6.109
3 SUAT 2.987 SAP 4.172 SUAT 4.25
4 MYC 2.8 ICM 3.774 NED 3.25
5 DICM 2.776 NED 3.674 MYC 3.125
VISAVET 3.279 4.841 6.681

ISI Scientific Publications
Total Last 60 mo.
1 Transbound Emerg Dis 93 Front Vet Sci 36
2 Vet Microbiol 88 Transbound Emerg Dis 33
3 Prev Vet Med 77 Animals 16
4 Front Vet Sci 61 Res Vet Sci 14
5 PLoS ONE 56 Sci Rep 14
  All journals 354   All journals 37