Últimas publicaciones científicas SCI
The expression of integron arrays is shaped by the translation rate of cassettes
Nature Communications 15(1):9232.
Integrons are key elements in the rise and spread of multidrug resistance in Gram-negative bacteria. These genetic platforms capture cassettes containing promoterless genes and stockpile them in arrays of variable length. In the current integron model, expression of cassettes is granted by the Pc promoter in the platform and is assumed to decrease as a function of its distance. Here we explored this model using a large collection of 136 antibiotic resistance cassettes and show the effect of distance is in fact negligible. Instead, cassettes have a strong impact in the expression of downstream genes because their translation rate affects the stability of the whole polycistronic mRNA molecule. Hence, cassettes with reduced translation rates d… Leer más
Carvalho A., Hipolito A., Trigo de Roza F., Garcia-Pastor L., Vergara E., Buendia A., Garcia-Seco T. y Escudero JA..
Herpesvirus surveillance in stranded striped dolphins (Stenella coeruleoalba) and bottlenose dolphins (Tursiops truncatus) from Italy with emphasis on neuropathological characterization
PLoS ONE 19(10):e0311767.
Herpesvirus (HV) is widely distributed among cetacean populations, with the highest prevalence reported in the Mediterranean Sea. In this study, a comprehensive analysis was conducted, including epidemiological, phylogenetic, and pathological aspects, with particular emphasis on neuropathology, to better understand the impact of HV in these animals. Our results show a higher presence of HV in males compared to females, with males exhibiting a greater number of positive tissues. Additionally, adults were more frequently affected by HV infection than juveniles, with no infections detected in calves or neonates. The affected species were striped (Stenella coeruleoalba) and bottlenose dolphins (Tursiops truncatus). The highest positivity rates … Leer más
Vargas-Castro I., Giorda F., Mattioda V., Goria M., Serraca L., Varello K., Carta V., Nodari S., Maniaci MG., dell´Atti L., Testori C., Pussini N., Iulini B., Battistini R., Zoppi S., Di Nocera F., Lucifora G., Fontanesi E., Acutis P., Casalone C., Grattarola C. y Peletto S.
Whole-genome sequencing of toxigenic Clostridioides difcile reveals multidrug resistance and virulence genes in strains of environmental and animal origin
BMC Veterinary Research 20(1):479.
Background
Clostridioides difficile has been recognized as an emerging pathogen in both humans and animals. In this context, antimicrobial resistance plays a major role in driving the spread of this disease, often leading to therapeutic failure. Moreover, recent increases in community-acquired C. difficile infections have led to greater numbers of investigations into the animal origin of the disease. The aim of this study was to evaluate the genetic similarities between 23 environmental and animal isolates by using whole-genome sequencing and to determine antimicrobial resistance and virulence factor genes in toxigenic C. difficile strains to provide important data for the development of diagnostic methods or treatment guidelines.
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Tercero-Guerrero D., Blanco JL., Hernandez M., Torre-Fuentes L., Alvarez J. y Garcia ME.
Serum total antioxidant status in dogs: Reference intervals and influence of multiple biological and analytical factors
Veterinary Clinical Pathology 1-10.
Background: Total antioxidant status (TAS) is one of the most widely used oxidative stress biomarkers, but the lack of canine RI and the influence of analytical factors hinder its application in clinical practice.
Objectives: The aims of this study were to establish canine assay-specific RI for TAS and evaluate the sources of biological variation and the association between TAS and multiple hematologic and biochemical variables.
Methods: Blood samples from 190 clinically healthy dogs were collected, encompassing pet dogs (82), police dogs (56), and shelter dogs (52). After hematologic and biochemical analysis, serum TAS was determined by means of a commercial 2,2`-azinobis (3-ethylbenzthiazolin-6-sulfonic acid) (ABTS) test. Th… Leer más
Perez-Montero B., Fermin-Rodriguez ML., Portero-Fuentes M., Sarquis J., Caceres S., Illera del Portal JC., de Juan L., Miro G. y Cruz F..
Chromosomal integrons are genetically and functionally isolated units of genomes
Nucleic Acids Research 1-7.
Integrons are genetic elements that increase the evolvability of bacteria by capturing new genes and stockpiling them in arrays. Sedentary chromosomal integrons (SCIs) can be massive and highly stabilized structures encoding hundreds of genes, whose function remains generally unknown. SCIs have co-evolved with the host for aeons and are highly intertwined with their physiology from a mechanistic point of view. But, paradoxically, other aspects, like their variable content and location within the genome, suggest a high genetic and functional independence. In this work, we have explored the connection of SCIs to their host genome using as a model the Superintegron (SI), a 179-cassette long SCI in the genome of Vibrio cholerae N16961. We have … Leer más
Blanco P., Trigo de Roza F., Toribio-Celestino L., Garcia-Pastor L., Caselli N., Moron A., Ojeda F., Darracq B., Vergara E., Amaro F., San Millan A., Skovgaard O., Mazel D., Loot C. y Escudero JA..
Identifying sites where wild boars can consume anthropogenic food waste with implications for African swine fever
PLoS ONE 19(8):e0308502.
Wild boar population dynamics promote the increase in numbers and distribution of the species in Eurasia, leading to a rise in the interaction with human activities, as well as generating problems with the management of certain infectious diseases, most notably African swine fever (ASF). ASF virus possesses high stability in several contaminated pork and pork products that can be a source of indirect transmission to susceptible hosts habituated to anthropogenic food waste. This transmission route is a concerning threat for the dispersion of the disease, primarily into unaffected areas given the worldwide widespread distribution of the disease and the increase of wild boar contact with humans. Thus, in this study, a straightforward tool to a… Leer más
Aguilar-Vega C., Sanchez-Vizcaino JM. y Bosch J.
Challenging boundaries: is cross-protection evaluation necessary for African swine fever vaccine development? A case of oral vaccination in wild boar
Frontiers In Immunology 15.
African swine fever (ASF) poses a significant threat to domestic pigs and wild boar (Sus scrofa) populations, with the current epidemiological situation more critical than ever. The disease has spread across five continents, causing devastating losses in the swine industry. Although extensive research efforts are ongoing to develop an effective and safe vaccine, this goal remains difficult to achieve.
Among the potential vaccine candidates, live attenuated viruses (LAVs) have emerged as the most promising option due to their ability to provide strong protection against experimental challenges. However, ASF virus (ASFV) is highly diverse, with genetic and phenotypic variations across different isolates, which differ in virulence. This… Leer más
Cadenas-Fernández E., Barroso-Arevalo S., Kosowska A., Diaz-de Frutos M., Gallardo A., Rodriguez-Bertos A., Bosch BJ., Sanchez-Vizcaino JM. y Barasona JA..
Novel Epidemiologic Features of High Pathogenicity Avian Influenza Virus A H5N1 2.3.3.4b Panzootic: A Review
Transboundary And Emerging Diseases 1-13.
Avian influenza is one of the most devastating avian diseases. The current high pathogenicity avian influenza (HPAI) A virus H5N1 clade 2.3.4.4b epizootic began in the 2020–2021 season, and has caused a panzootic, considered one of the worst ever reported. The present panzootic has novel epidemiological features that represent a challenge for its prevention and control. This review examines key epidemiological changes of the disease such as seasonality, geographic spread, and host range. The seasonality of the virus has changed, and contrary to previous avian influenza epizootics, this subclade was able to persist during boreal summer. Its geographic range has expanded, with reports in all continents except Australia. During this epizootic,… Leer más
Sacristan C., Ewbank AC., Ibanez-Porras P., Perez-Ramirez E., de la Torre A., Briones V. y Iglesias C.
Stream of revelation: detection of equine herpesvirus-1 (EHV-1) in urine during myeloencephalopathy outbreaks
Equine Veterinary Journal 56(S60)67-67.
Background: The detection of EHV-1 by real-time PCR in nasal swabs (NS), whole blood, brain, and spinal cord samples has been extensively described. However, there remains a notable gap in understanding the excretion of the virus in urine, the patterns of DNA detection, and the potential role of urine in viral dissemination during an outbreak.
Objectives: To ascertain the presence of EHV-1 DNA in urine during natural infection and to compare the DNA detection patterns of EHV-1 in urine, buffy coat (BC), and NS.
Study design: Prospective clinical study.
Methods: During the hospitalisation of twenty-one horses involved in two EHV-1 myeloencephalopathy outbreaks in Spain in 2021 and 2023, urine, who… Leer más
Velloso-Alvarez A., Cunilleras EJ., Dorrego-Rodriguez A., Santiago-Llorente I., Cuesta M., Troya-Portillo L., Rivera B., de Juan L. y Cruz F..
Genomic comparison between Mycobacterium bovis and Mycobacterium microti and in silico analysis of peptide-based biomarkers for serodiagnosis
Frontiers In Veterinary Science 11:1446930.
In recent years, there has been an increase in the number of reported cases of Mycobacterium microti infection in various animals, which can interfere with the ante-mortem diagnosis of animal tuberculosis caused by Mycobacterium bovis. In this study, whole genome sequencing (WGS) was used to search for protein-coding genes to distinguish M. microti from M. bovis. In addition, the population structure of the available M. microti genomic WGS datasets is described, including three novel Belgian isolates from infections in alpacas. Candidate genes were identified by examining the presence of the regions of difference and by a pan-genome analysis of the available WGS data. A total of 80 genes showed presence-absence variation between the two spe… Leer más
Moens C., Bogaerts B., Lorente-Leal V., Vanneste K., Keersmaeecker SCJ., Roosens NHC., Mostin L., Fretin D. y Marche S.