Últimas publicaciones científicas SCI
Application of machine learning with large-scale data for an effective vaccination against classical swine fever for wild boar in Japan
Scientific Reports 14(1):5312.
Classical swine fever has been spreading across the country since its re-emergence in Japan in 2018. Gifu Prefecture has been working diligently to control the disease through the oral vaccine dissemination targeting wild boars. Although vaccines were sprayed at 14,000 locations between 2019 and 2020, vaccine ingestion by wild boars was only confirmed at 30% of the locations. Here, we predicted the vaccine ingestion rate at each point by Random Forest modeling based on vaccine dissemination data and created prediction surfaces for the probability of vaccine ingestion by wild boar using spatial interpolation techniques. Consequently, the distance from the vaccination point to the water source was the most important variable, followed by elev… Leer más
Ito S., Aguilar-Vega C., Bosch J., Isoda N. y Sanchez-Vizcaino JM..
Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats
Veterinary Sciences 11(3):105.
Paratuberculosis (PTB) and tuberculosis (TB) are two mycobacterial diseases with a severe economic and health impact on domestic ruminants. The ante mortem diagnosis of PTB is hampered, among other factors, by the limited sensitivity of all the available diagnostic techniques. Since TB-infected goats subjected to the comparative intradermal tuberculin test (CITT) may experience a booster effect on their antibody titer and a potential enhancement to the sensitivity of humoral techniques for tuberculosis, in the present study we aimed to evaluate this diagnostic strategy on the humoral diagnosis of PTB in serum and milk samples collected from a caprine herd that was TB free and PTB infected. The results from 120 goats indicated a significant … Leer más
Velasco-Reinaldos C., Ortega J., Gómez-Buendía A., Grau A., Lopez M., Alvarez J., Romero B., de Juan L. y Bezos J..
Phenotypic and genetic characterization of antimicrobial resistance in Salmonella enterica serovar Choleraesuis isolates from humans and animals in Spain from 2006 to 2021
The Journal Of Antimicrobial Chemotherapy 1-11.
Objectives
While an increase in the levels of MDR in Salmonella enterica sevorar Choleraesuis has been reported in Europe, little is known about the situation in Spain. Therefore, we first aimed to assess the phenotypic resistance profile and to determine the presence of genetic determinants of resistance of S. Choleraesuis isolates collected in animal and human. Our second objective was to identify and characterize clusters of highly related isolates.
Methods
We analysed 50 human and 45 animal isolates retrieved from 2006 to 2021 using the disc diffusion method and performed WGS followed by analyses of genetic determinants and phylogenetic analysis.
Results
All isolates were of ST145 and c… Leer más
Jacqueline C., Samper-Cativiela C., Monzon S., Ugarte-Ruiz M., Cuesta I., Alvarez J. y Herrera-Leon S.
Landscape connectivity for predicting the spread of ASF in the European wild boar population
Scientific Reports 10;14(1):3414.
African swine fever (ASF) is an infectious and highly fatal disease affecting wild and domestic swine, which is unstoppably spreading worldwide. In Europe, wild boars are one of the main drivers of spread, transmission, and maintenance of the disease. Landscape connectivity studies are the main discipline to analyze wild-species dispersal networks, and it can be an essential tool to predict dispersal-wild boar movement routes and probabilities and therefore the associated potential ASF spread through the suitable habitat. We aimed to integrate wild boar habitat connectivity predictions with their occurrence, population abundance, and ASF notifications to calculate the impact (i.e., the capacity of a landscape feature to favor ASF spread) an… Leer más
Goicolea T., Cisneros-Araujo P., Aguilar-Vega C., Sanchez-Vizcaino JM., Mateo-Sanchez MC. y Bosch J.
Forensic investigations of suspected livestock depredation by vultures: scientific tools for compensation programmes
Journal Of Comparative Pathology 209:22-30.
Human–wildlife conflicts may have devastating consequences for fauna due to targeting by humans of wildlife populations suspected to have predated livestock. Suspicion of depredation of extensively raised livestock by vultures in Europe has triggered public administration-led forensic investigations intended to distinguish between predation and scavenging in order to compensate farmers for attacks on their livestock. In this study, gross and histological analyses were carried out on suspected cases of domestic animal depredation by griffon vultures (Gyps fulvus) over a 1-year period. Fifty-eight animals were affected (n = 41, suspected depredation), including domestic cattle (75.9%), sheep (22.4%) and a goat (1.7%). All the adults affected … Leer más
Rebollada A., Gómez-Buendía A., Dominguez L. y Rodriguez-Bertos A..
Effect of a recent intradermal test on the specificity of P22 ELISA for the diagnosis of caprine tuberculosis
Frontiers In Veterinary Science 11:1-7.
Caprine tuberculosis (TB) is a zoonotic disease caused by members of the Mycobacterium tuberculosis complex. TB eradication programs in goats are based on the single and comparative intradermal tuberculin tests (SITT and CITT, respectively). Antibody-based diagnostic techniques have emerged as potential diagnostic tools for TB. P22 ELISA has been previously evaluated using samples collected after the intradermal tuberculin tests to maximize the sensitivity, a phenomenon known as booster effect. However, there is no information available on whether the use of this diagnostic strategy could lead to a decrease of its specificity (Sp). The aim of the present study was to elucidate the interference effect of a recent CITT on the Sp of the P22 EL… Leer más
Velasco-Reinaldos C., Ortega J., Rincón-Fernández de la Puente J., Romero B., de Juan L., Dominguez L., Dominguez M., Moreno I., Alvarez J. y Bezos J..
Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds
Frontiers In Veterinary Science 11:1361788.
Non-tuberculous mycobacteria (NTM) are considered a relevant cause of non-specific reactions to the most widely applied bovine tuberculosis (bTB) test, the intradermal tuberculin test. In order to establish which NTM species might act as a potential source of such diagnostic interference, a collection of 373 isolates obtained from skin test positive cows from 359 officially tuberculosis-free (OTF) herds, culled in the framework of the bTB eradication campaign in Spain, were identified at the species level through PCR and Sanger sequencing of the 16S rDNA, hsp65 and rpoB genes. Of the 308 isolates for which a reliable identification was achieved, 32 different mycobacterial species were identified, with certain species being most represented:… Leer más
Gómez-Buendía A., Alvarez J., Bezos J., Mourelo J., Amado J., Saez-Llorente JL., de Juan L. y Romero B..
Detection of Brucella in Dermacentor ticks of wild boar with brucellosis
Transboundary And Emerging Diseases 2024:5517000, 12 pag.
Brucellosis is a sanitary and economically relevant disease affecting humans, livestock, and wildlife. Ticks have been suggested as vectors, long-term carriers, and amplifiers of Brucella. In this study, ticks from wildlife ungulate hosts living in hunting reserves of a central region of Spain were collected during a 6-year period, pooled, and screened for Brucella spp. by PCR. Aiming to correlate Brucella spp. DNA presence in ticks with Brucella spp. infections in wildlife ungulate hosts, liver samples from deceased wildlife ungulates coming from the hunting reserves showing a positive result for Brucella in ticks were tested using a commercial ELISA. In total, 229 tick pools from wild boar (Sus scrofa, n = 176; 76.8%, 95% CI 7… Leer más
Rebollada A., Martinez I., Duque C., Garcia-Seco T., Escacena C., Dominguez L., Rodriguez-Bertos A. y Garcia N..
Identification of promoter activity in gene-less cassettes from Vibrionaceae superintegrons
Nucleic Acids Research 1-16.
Integrons are genetic platforms that acquire new genes encoded in integron cassettes (ICs), building arrays of adaptive functions. ICs generally encode promoterless genes, whose expression relies on the platform-associated Pc promoter, with the cassette array functioning as an operon-like structure regulated by the distance to the Pc. This is relevant in large sedentary chromosomal integrons (SCIs) carrying hundreds of ICs, like those in Vibrio species. We selected 29 gene-less cassettes in four Vibrio SCIs, and explored whether their function could be related to the transcription regulation of adjacent ICs. We show that most gene-less cassettes have promoter activity on the sense strand, enhancing the expression of downstream cassettes. Ad… Leer más
Blanco P., Hipolito A., Garcia-Pastor L., Trigo de Roza F., Toribio-Celestino L., Ortega AC., Vergara E., San Millan A. y Escudero JA..
Heat-inactivated mycobacteria activate the toll-like receptor 2 and 4 pathways in the zebrafish model of tuberculosis
Vaccine S0264-410X(23)01544-X.
Based on previous evidence demonstrating the efficacy of inactivated mycobacteria for the control of fish mycobacteriosis, we explored the protective efficacy of two inactivated Mycobacterium bovis administered via parenteral and mucosal routes against Mycobacterium marinum infection mimicking natural conditions in the zebrafish model of tuberculosis. Although we did not observe a clear effect of any of the immunostimulants on mycobacterial burden, the results showed a significant increase in TLR2 and TLR4 gene expression levels in fishes parenterally immunized with inactivated Bacillus Calmette-Guérin (BCG). Our findings demonstrated that the TLR2 and the TLR4 signaling pathways are involved in the immune response elicited by inactivated m… Leer más
Ferreras E., Contreras M., Risalde MA., Sevilla IA., Delgado E., Dominguez L., Gortazar C. y de la Fuente J.