Últimas publicaciones científicas SCI
Current concepts and contents of Veterinary Public Health and Veterinary Health Polic
Revista Española De Salud Pública 92:e201810077.
Human and veterinary medicine obviously share most concepts and contents. However, both academic and scientific issues and the daily practice of many health disciplines can differ largely between human and veterinary medicine. Such differences can easily go unnoticed among other healthcare collectives or the general public. The aim of the present work was to provide an update, as well as some definitions of what we understand under the terms Veterinary Public Health and Veterinary Health Policy… Leer más
Briones V., Bezos J. y Alvarez J..
Ant(6)-I genes encoding aminoglycoside O-nucleotidyltransferases are widely spread among streptomycin resistant strains of Campylobacter jejuni and Campylobacter coli
Frontiers In Microbiology 9:2515.
Thermotolerant Campylobacter species C. jeuni and C. coli are actually recognized as the major bacterial agent responsible for food-transmitted gastroenteritis. The most effective antimicrobials against Campylobacter are macrolides and some, but not all aminoglycosides. Among these, susceptibility to streptomycin is reduced by mutations in the ribosomal RPSL protein or by expression of ANT(6)-I aminoglycoside O-nucleotidyltransferases. The presence of streptomycin resistance genes was evaluated among streptomycin-resistant Campylobacter isolated from humans and animals by using PCR with degenerated primers devised to distinguish ant(6)-Ia, ant(6)-Ib and other ant-like genes. Genes encoding ANT(6)-I enzymes were found in all possible combina… Leer más
Hormeno Garcia L., Ugarte-Ruiz M., Palomo G., Borge C., Vadillo Machota S., Piriz S., Dominguez L., Jorge Campos M. y Quesada A.
Pathologic findings and causes of death of stranded cetaceans in the Canary Islands (2006-2012)
PLoS ONE 13(10): e0204444.
This study describes the pathologic findings and most probable causes of death (CD) of 224 cetaceans stranded along the coastline of the Canary Islands (Spain) over a 7-year period, 2006-2012. Most probable CD, grouped as pathologic categories (PCs), was identified in 208/224 (92.8%) examined animals. Within natural PCs, those associated with good nutritional status represented 70/208 (33.6%), whereas, those associated with significant loss of nutritional status represented 49/208 (23.5%). Fatal intra- and interspecific traumatic interactions were 37/208 (17.8%). Vessel collisions included 24/208 (11.5%). Neonatal/perinatal pathology involved 13/208 (6.2%). Fatal interaction with fishing activities comprised 10/208 (4.8%). Within anthropoge… Leer más
Diaz-Delgado J., Fernandez A., Sierra E., Sacchini S., Andrada M., Vela AI., Quesada-Canales O., Paz Y., Zucca D., Groch K. y Arbelo M.
Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level
Journal Of Fish Diseases 41(10):1485-1493.
The aim of this study was to evaluate the usefulness of the MALDI-TOF MS to identify 151 isolates of Aeromonas obtained mostly from diseased fish. MALDI-TOF MS correctly identified all isolates to the genus level but important differences in the percentage of isolates correctly identified depending on the species were observed. Considering exclusively the first identification option, Aeromonas bestiarum, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas veronii and Aeromonas sobria were the best identified with results >95%. However, considering the first and second identification options, the only species that showed values >90% was A. hydrophila. Overall, when the database was supplemented with 14 new spectra, the number of accurate … Leer más
Perez-Sancho M., Cerda I., Fernandez-Bravo A., Dominguez L., Figueras MJ., Fernandez-Garayzabal JF. y Vela AI..
Management of hunting waste as control measure for tuberculosis in wild ungulates in south-central Spain
Transboundary And Emerging Diseases 65(5):1190-1196.
In recent decades, habitat change and the intensive management of wild ungulates for hunting have led to an increase in their populations in south-central Spain. This implies a higher generation of hunting waste, which can favour the transmission of infectious diseases, including tuberculosis (TB). The aim of this study was to assess the usefulness of the proper disposal of hunting waste as TB control measure in wild boar (Sus scrofa) and red deer (Cervus elaphus) during the 2008/2009 to 2016/2017 hunting seasons. Blood samples from 664 wild boar and 934 red deer were obtained in 14 game estates in two provinces in Andalusia (Area 1), where the disposal of hunting waste was implemented since the 2012/2013 hunting season. Besides, six game e… Leer más
Cano-Terriza D., Risalde MA., Jiménez-Ruiz S., Vicente J., Isla J., Paniagua J., Moreno I., Gortazar C., Infantes-Lorenzo JA. y Garcia-Bocanegra I.
Carriage of antibiotic-resistant bacteria in urban versus rural wild boars
European Journal Of Wildlife Research (2018) 64: 60.
The Western European population of wild boar (Sus scrofa) has increased its distribution over the past several decades, and some populations have colonized areas strongly influenced by human activity. Wild boars are known carriers of antibiotic-resistant bacteria acquired from the environment, and urban populations of wild boars may be more exposed than their rural counterparts. In this work, we compared the frequency of antibiotic resistance in indicator bacteria (Escherichia coli, Enterococcus faecalis, Enterococcus faecium) isolated from urban wild boars with that from rural wild boars in NE Spain. We further assessed whether bacterial isolates from the urban wild boars had a higher probability of showing antibiotic resistance when their… Leer más
Navarro Gonzalez N., Castillo Contreras R., Casas Diaz E., Morellet N., Porrero MC., Molina Vacas G., Torres RT., Fonseca C., Mentaberre G., Dominguez L., Lavin S. y Serrano E.
Evaluation of the performance of the IDvet IFN-gamma test for diagnosis of bovine tuberculosis in Spain
Frontiers In Veterinary Science 229(5):1-9.
In Spain, the national bovine tuberculosis (bTB) eradication program is based on yearly skin testing of every ≥6 weeks old animal using the single or comparative tuberculin test and parallel use of the interferon-gamma (IFN-γ) assay as an ancillary diagnostic test in infected herds. There are several versions of the latter. Recently, a new commercial IDvet IFN-γ assay has been authorized for use in the program, but there is limited scientific evidence about its performance in different epidemiological settings. Therefore, two studies to evaluate the performance of the IDvet assay were conducted. In study 1, a concordance analysis between the new IDvet and the Bovigam IFN-γ assay in use in Spain for over 10 years was con… Leer más
de la Cruz ML., Branscum AJ., Nacar J., Pages E., Pozo P., Perez AM., Grau A., Saez JL., de Juan L., Diaz R., Minguez O. y Alvarez J..
Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguays University Hospital Identifies the First rmtC Gene in the Species
Microbial Drug Resistance (Larchmont, N.Y.) 24(7):1012-1019.
Carbapenem-resistant Acinetobacter baumannii (CRAB) infections are an increasing concern in intensive care units (ICUs) worldwide. The combination of carbapenemases and 16S rRNA-methyltransferases (16S-RMTases) further reduces the therapeutic options. OXA-carbapenemase/A. baumannii clone tandems in Latin America have already been described; however, no information exists in this region regarding the occurrence of 16S-RMTases in this microorganism. In addition, the epidemiology of A. baumannii in ICUs and its associated resistance profiles are poorly understood. Our objectives were as follows: to study the clonal relationship and antibiotic resistance profiles of clinical and digestive colonizing A. baumannii isolates in an ICU, to character… Leer más
Bado I., Papa-Ezdra R., Delgado-Blas JF., Gaudio M., Gutierrez C., Cordeiro NF., Garcia-Fulgueiras V., Pirez LA., Seija V., Medina JC., Rieppi G., Gonzalez-Zorn B. y Vignoli R.
Novel and highly sensitive sybr® green real-time pcr for poxvirus detection in odontocete cetaceans
Journal Of Virological Methods 259(2018)45-49.
Poxviruses are emerging pathogens in cetaceans, temporarily named `Cetaceanpoxvirus` (CePV, family Poxviridae), classified into two main lineages: CePV-1 in odontocetes and CePV-2 in mysticetes. Only a few studies performed the molecular detection of CePVs, based on DNA-polymerase gene and/or DNA-topoisomerase I gene amplification. Herein we describe a new real-time PCR assay based on SYBR® Green and a new primer set to detect a 150 bp fragment of CePV DNA-polymerase gene, also effective for conventional PCR detection. The novel real-time PCR was able to detect 5 up to 5 × 106 copies per reaction of a cloned positive control. Both novel PCR methods were 1000 to 100,000-fold more sensitive than those previously described in the l… Leer más
Sacristan C., Luiz Catao-Dias J., Ewbank AC., Machado EF., Neves E., Santos-Neto EB., Avezedo A., Laison-Brito J., De Castillo PV., Daura-Jorge FG., Simoes-Lopes PC., Carballo M., Garcia-Parraga D., Sanchez-Vizcaino JM. y Esperon F.
MultiOrgan Disease and Death Associated With Streptococcus equi spp. zooepidemicus in a 2-Month-Old Foal
Journal Of Equine Veterinary Science 70(2018):112-116.
A 2-month-old Andalusian colt in central Spain presented dyspnea, diarrhea, and depression. Despite the initial treatment for presumptive pneumonia caused by Rhodococcus equi, after 1 hour, the colt showed signs of shock and died. The necropsy revealed multiple abscesses in the lung, as well as enlarged mesenteric lymph nodes, and hemorrhagic peritonitis. Samples were taken from trachea, lung parenchyma, small intestine, and colon. All samples yielded a pure culture of Streptococcus equi spp. zooepidemicus, whereas R. equi was excluded by using a specific qPCR performed on all samples. Isolates from all samples were characterized by pulse-field gel electrophoresis and the representative strain was identified by MALDI-TOF and qPCR. Its genom… Leer más
Dominguez-Gimbernat M., Florez-Cuadrado D., Steward K., Waller A., Porrero MC., Vela AI., Perez-Sancho M., de Juan L. y Cruz F..
