Últimas publicaciones científicas SCI
Evaluation of serological assays for intra vitam diagnosis of bovine tuberculosis in water buffalo (Bubalus bubalis)
Frontiers In Microbiology 16.
Introduction: Bovine tuberculosis (TB) in water buffalo (Bubalus bubalis) is primarily diagnosed using intra vitam tests, as the intradermal tuberculin test (IDT) and the interferon-gamma release assay (IGRA), both of which detect cell-mediated immunity (CMI). However, a subset of infected animals fails to mount a detectable CMI response, posing a significant risk of undetected transmission. Serological tests assessing the humoral immune response could provide a valuable complementary tool for identifying infected animals that escape detection through traditional CMI-based assays.
Methods: This study analyzed 895 serum samples from water buffaloes, including 393 from TB-free herds and 502 from TB-infected herds. Animals from TB-free … Leer más
Giovanozzi S., Martucciello A ., Dominguez M., Moreno I., Boifava M., Schiavo L., Viscito A., Parisio G., Vitale N., Bezos J., De Carlo E. y Boniotti MB.
Persistent multispecies dissemination of armA-carrying IncR plasmids among clinical and environmental bacterial populations in a Spanish veterinary hospital
Journal Of Clinical Microbiology e0067325.
Aminoglycoside resistance mediated by 16S rRNA methyltransferases poses a growing threat in both human and veterinary medicine. Here, we conducted a retrospective genomic study of Enterobacterales isolates (n = 789) collected from clinical samples at a veterinary teaching hospital in Spain between 2011 and 2020. We identified four high-level aminoglycoside-resistant ST171 Enterobacter hormaechei subsp. xiangfangensis isolates carrying the armA gene, all from horses. Using Illumina and Nanopore sequencing, we determined that armA was located on a 70 kb IncR plasmid, also carrying other resistance genes such as msr(E), mph(E), blaDHA-1, and qnrB4, embedded within a Tn1548-like element. Genomic comparisons indicated that the IncR plasmid was l… Leer más
Serna C., Pulido-Vadillo M., Matamoros BR., Fernandez-Favieres FJ., Montero N., Garcia-Berdun C., Garcia ME., Blanco JL., Delgado-Blas JF. y Gonzalez-Zorn B..
A time-space Bayesian regression model of rabies cases in the animal population of Kazakhstan (2013-2023)
Frontiers In Veterinary Science 12:1-9.
Despite its endemic status and socioeconomic impacts, the spatial-temporal variation in rabies risk and its underlying determinants in Kazakhstan animal populations remain poorly understood. This study aimed to characterise the time-space dynamics of rabies in animal populations across Kazakhstan regions from 2013 to 2023 and identify the key drivers of transmission. Using a Bayesian hierarchical regression model with spatial and temporal random effects, we analysed national surveillance data on rabies cases in livestock, companion animals, and wildlife, alongside sociodemographic and animal population variables. The model revealed that higher median income (odds ratio [OR]: 1.18, 95% posterior predictive interval [PPI]: 1.06–1.31), the p… Leer más
Gómez-Buendía A., Yessembekova G., Kadyrov A., Mukhanbetkaliyev Y., Cervino-Luridiana E., Alvarez J., Perez AM. y Abdrakhmanov SK.
Protective effect of heat-inactivated Mycobacterium bovis applied intramuscularly is associated with enhanced lung immune response in caprine tuberculosis
Veterinary Research 56(1):209.
Caprine tuberculosis (TB) causes a zoonotic disease with significant economic and health implications. However, excluding some regions, goat herds are not subjected to official TB eradication programs. Implementing vaccination protocols for this species could provide a complementary and effective control strategy against TB. We assessed the protective efficacy and immune response associated with a heat-inactivated Mycobacterium bovis (M. bovis)-based immunostimulant (HIMB) applied intramuscularly against caprine pulmonary TB on 20 kid goats (10 immunized, 10 controls) naturally exposed to M. caprae infected goats for 10 months. TB-compatible lung lesions were assessed, alongside a local immune response analysis by immunohistochemistry of ce… Leer más
Agullo-Ros I., Munoz-Fernandez L., Roy A., Bezos J., Sevilla IA., Moreno I., Garrido J., Rodriguez-Bertos A., Dominguez M., Juste R., Dominguez L., Gortazar C. y Risalde MA.
Diving Into the Depths: Unveiling the Main Etiologies of Piscine Lactococcosis With a Novel Multiplex qPCR Assay
Journal Of Fish Diseases 48(11):e14147.
Piscine lactococcosis poses a significant threat to a wide range of cultured and wild fish populations worldwide, typically presenting as acute haemorrhagic septicemia with high morbidity and mortality. Although Lactococcus garvieae was historically considered the sole causative agent of piscine lactococcosis, recent studies have identified L. petauri and L. formosensis as additional, highly pathogenic species. In this study, we developed a novel TaqMan-based multiplex qPCR assay for the simultaneous detection and differentiation of L. garvieae, L. petauri and L. formosensis, following a pangenome analysis of the publicly available genomes of these bacterial species. The assay demonstrated high sensitivity and specificity across 156 bacteri… Leer más
Shahin K., Abdel-Glil M., Burcin Saticioglu I., Duman M., Altun S., Colussi S., Esposito G., Acutis PL., Marino P., Spondler B., Altinok I., Kotzamanidis C., Vela AI., Soto E., Gomes-Leal CA., Ajmi N. y Aoki S.
Healthcare-related transmission of mobile genetic elements co-carrying blaNDM and 16S rRNA methyltransferase genes in multiple Enterobacterales
Microbial Genomics 11(8):001473.
Aminoglycosides are used in the treatment of serious infections with Gram-negative bacteria, especially those resistant to beta-lactams and carbapenems. 16S rRNA methyltransferases (16S-RMTase) are capable of conferring resistance to nearly all aminoglycosides. They are sometimes detected in combination with bla NDM. This study describes the mobile genetic elements associated with bla NDM and 16S-RMTase (co-)carriage in Enterobacterales from Ireland in the period 2019-2023. All isolates (n=58) carrying both bla NDM and a 16S-RMTase gene between 2019 and 2023 were obtained from the CPE National Reference Laboratory Service. Short-read sequences were generated for all isolates, and long-read sequences were generated for a subset of isolates (… Leer más
Maguire M., Serna C., Delgado-Blas JF., Clarke C., DeLappe N., Cormican M., Coughlan SC., Miliotis G., Gonzalez-Zorn B. y Burke LP.
WiBISS: a tool to estimate avoided lost revenue of African swine fever wild boar vaccination at municipality level
Frontiers In Veterinary Science 12:1667173.
This study introduces the WiBISS model, a simulation tool designed to assess the economic and epidemiological impact of a hypothetical African Swine Fever (ASF) vaccination in wild boar in Northern Italy. Using real ASF outbreak data from January 2022 to June 2024, the model evaluates how prompt vaccination could reduce disease spread and economic losses. WiBISS integrates three modules: vaccination simulation, restriction zone estimation, and economic impact analysis. The first two use custom-built cellular automata (CA) in Python and ArcGIS Pro, modeling each ASF case as a cell that can be in one of three states: unvaccinated, infected, or vaccinated. Weekly iterations over 2.5 years simulate ASF progression and vaccination impact based o… Leer más
Ibanez-Porras P., Bosch J., Feliziani F., Maresca C., Sanchez-Vizcaino JM., Iglesias I., Aguilar-Vega C. y Martinez-Aviles M.
Primary leptomeningeal histiocytic lymphoproliferative disorder associated with SARS-CoV-2 brain infection in k18-hACE2 mouse: a case report
Laboratory Animal Research 41(1):23.
Background: Histiocytic proliferative disorders in the central nervous system are rare, and their potential association with viral infections remains largely unexplored. This case is relevant because it suggests a potential interaction between SARS-CoV-2 neuroinvasion and tumor development, providing insights into how viral infections might influence oncogenesis.
Case presentation: A 4.5-month-old male k18-hACE-2 mouse, part of an experimental study of SARS-CoV-2, displayed a small mass in leptomeningeal area composed by neoplastic round cells. This process is associated with typical acute inflammatory and neurodegenerative lesions according to viral neuroinvasion. Histopathology revealed a well-demarcated tumor composed of lymphobla… Leer más
Porras N., Sánchez-Morales L., Perez-Sancho M., Dominguez L. y Rodriguez-Bertos A..
Evidence of ESBL plasmid transfer and selective persistence of multiple host-associated Escherichia coli isolates in a chicken cecal fermentation model
Applied And Environmental Microbiology 91(10):e0082225.
The guts of animals and humans harbor diverse microbial communities that are regularly exposed to bacteria originating from food, water, and their surroundings. Species such as Escherichia coli are adept at colonizing multiple hosts, along with surviving in the environment. By encoding pathogenic traits and transmissible forms of antimicrobial resistance (AMR), E. coli can also pose a zoonotic risk. Our understanding of the factors that govern host residency is limited. Here, we used a chicken cecal fermentation model to study survival and the AMR transfer potential of 17 host-associated extended-spectrum ?-lactamase (ESBL)-producing E. coli isolates. Vessels containing chicken cecal contents were stabilized for 4 days before the addition o… Leer más
Leng J., Ferrandis-Vila M., Oldenkamp R., Mehat JW., Fivian-Hughes A., Kumar-Tiwari S., Van der Putten B., Trung-Nguyen V., Bethe A., Clark J., Singh P., Semmler T., Schwarz S., Alvarez J., Hoa NT., Bootsma M., Menge C., Berens C., Schultsz C., Ritchie JM. y La Ragione R.
Evolution of Suspected Cat Abuse Between 2020 and 2024 in the Community of Madrid (Spain)
Animals 15:2892.
Animal abuse is a serious and underreported problem, with cats, particularly those living in colonies, being frequent victims. In Spain, reliable data on feline abuse are limited. This study evaluated the cause, manner and mechanisms of death from 53 cats over five years at the Pathology and Forensic Veterinary Unit of the VISAVET Health Surveillance Center in Madrid (Spain). More than half of the cats died from non-natural causes, including blunt force trauma, poisoning, and projectile injuries, while the remaining cats died from natural
causes. Compared to a previous regional study, suspected abuse cases have increased, including new instances of antifreeze poisoning. These findings provide insight into trends in feline abuse and u… Leer más
Aradilla-Macias N., De Pablo-Moreno J., Porras N., Chinchilla-Rodríguez B. y Rodriguez-Bertos A..
