Últimas publicaciones científicas ISI
Vaccination as a control strategy against Salmonella infection in pigs: A systematic review and meta-analysis of the literature
Research In Veterinary Science 114(2017)86-94.
Consumption or handling of improperly processed or cooked pork is considered one of the top sources for foodborne salmonellosis, a common cause of intestinal disease worldwide. Asymptomatic carrier pigs may contaminate pork at slaughtering; therefore, pre-harvest reduction of Salmonella load can contribute to reduce public health risk. Multiple studies have evaluated the impact of vaccination on controlling Salmonella in swine farms, but results are highly variable due to the heterogeneity in vaccines and vaccination protocols. Here, we report the results of an inclusive systematic review and a meta-analysis of the peer-reviewed scientific literature to provide updated knowledge on the potential effectiveness of Salmonella vaccination. A to… Leer más
de la Cruz ML., Conrado I., Nault A., Perez A., Dominguez L. y Alvarez J.
How does temperature influences the development of lactococcosis? Transcriptomic and immunoproteomic in vitro approaches
Journal Of Fish Diseases 40(10):1285-1297.
Lactococcus garvieae is the aetiological agent of lactococcosis, a haemorrhagic septicaemia that affects marine and freshwater fish, with special incidence and economic relevance in farmed rainbow trout. Water temperature is one of the most important predisposing factors in the development of lactococcosis outbreaks. Lactococcosis in trout usually occur when water temperatures rise to about 18 °C, while fish carriers remain asymptomatic at temperatures below 13 °C. The aim of this work was to analyse the differences in the complete transcriptome response of L. garvieae grown at 18 °C and at 13 °C and to identify the immunogenic proteins expressed by this bacterium at 18 °C. Our results show that water temperature influences the expression o… Leer más
Castro R., Reguera-Brito M., Lopez-Campos GH., Blanco MM., Aguado-Urda M., Fernandez-Garayzabal JF. y Gibello A.
Update on the Risk of Introduction of African Swine Fever by Wild Boar into Disease-Free European Union Countries
Transboundary And Emerging Diseases 64(5):1424-1432.
Despite efforts to prevent the appearance and spread of African swine fever (ASF) in the European Union, several Member States are now affected (Lithuania, Poland, Latvia and Estonia). Disease appearance in 2014 was associated with multiple entrances linked to wild boar movement from endemic areas (EFSA Journal, 8, 2015, 1556), but the risk of new introductions remains high (Gallardo et al., Porcine Health Management, 1, and 21) as ASF continues to be active in endemic countries (Russian Federation, Belarus and Ukraine). Since 2014, the number of ASF notifications has increased substantially, particularly in wild boar (WB), in parallel with slow but constant geographical advance of the disease. This situation suggests a real risk of further… Leer más
Bosch J., Rodriguez A., Iglesias I., Munoz MJ., Jurado C., Sanchez-Vizcaino JM. y de la Torre A.
Parenteral Vaccination with Heat-Inactivated Mycobacterium bovis Reduces the Prevalence of Tuberculosis-Compatible Lesions in Farmed Wild Boar
Transboundary And Emerging Diseases 64(5):e18-e21.
In 2012, a wild boar (Sus scrofa) tuberculosis (TB) control programme was set up in a wild boar farm by means of intramuscular (IM) vaccination with a heat-inactivated Mycobacterium bovis vaccine (IV). The goal was to assess safety and efficacy of the parenterally administered IV in a large farm setting with natural M. bovis circulation. Based on preceding results under laboratory conditions, we hypothesized that vaccinated piglets would show smaller scores of TB-compatible lesions (TBCL) than unvaccinated controls. After vaccination, no adverse reactions were detected by visual inspection or at post-mortem examination (n = 668 and 97, respectively). Post-mortem data on TBCL were available for 97 vaccinated wild boar and 182 controls. The o… Leer más
Diez-Delgado I., Rodriguez O., Boadella M., Garrido JM., Sevilla I., Bezos J., Juste R., Dominguez L. y Gortazar C.
Identification of a New Genotype of African Swine Fever Virus in Domestic Pigs from Ethiopia
Transboundary And Emerging Diseases 64(5):1393-1404.
African swine fever (ASF) is an important emerging transboundary animal disease (TAD), which currently has an impact on many countries in Africa, Eastern Europe, the Caucasus and the Russian Federation. The current situation in Europe shows the ability of the virus to rapidly spread, which stands to threaten the global swine industry. At present, there is no viable vaccine to minimize spread of the disease and stamping out is the main source of control. In February 2011, Ethiopia had reported its first suspected outbreaks of ASF. Genomic analyses of the collected ASF virus (ASFV) strains were undertaken using 23 tissue samples collected from domestic swine in Ethiopia from 2011 to 2014. The analysis of Ethiopian ASFVs partial p72 gene seque… Leer más
Achenbach JE., Gallardo C., Nieto-Pelegrin E., Rivera B., Degefa T., Arias M., Jenberie S., Mulisa DD., Gizaw D., Gelaye E., Chibssa TR., Belaye A., Loitsch A., Forsa M., Yami M., Diallo A., Soler A., Lamien CE. y Sanchez-Vizcaino JM..
Serosurvey Reveals Exposure to West Nile Virus in Asymptomatic Horse Populations in Central Spain Prior to Recent Disease Foci
Transboundary And Emerging Diseases 64(5):1387-1392.
West Nile fever/encephalitis (WNF) is an infectious disease affecting horses, birds and humans, with a cycle involving birds as natural reservoirs and mosquitoes as transmission vectors. It is a notifiable disease, re-emerging in Europe. In Spain, it first appeared in horses in the south (Andalusia) in 2010, where outbreaks occur every year since. However, in 2014, an outbreak was declared in horses in central Spain, approximately 200 km away from the closest foci in Andalusia. Before that, evidence of West Nile virus (WNV) circulation in central Spain had been obtained only from wildlife, but never in horses. The purpose of this work was to perform a serosurvey to retrospectively detect West Nile virus infections in asymptomatic horses in … Leer más
Abad-Cobo A., Llorente F., Barbero MD., Cruz F., Fores P. y Jimenez-Clavero MA.
Motion-based video monitoring for early detection of livestock diseases: The case of African swine fever
PLoS ONE 12(9):e0183793.
Early detection of infectious diseases can substantially reduce the health and economic impacts on livestock production. Here we describe a system for monitoring animal activity based on video and data processing techniques, in order to detect slowdown and weakening due to infection with African swine fever (ASF), one of the most significant threats to the pig industry. The system classifies and quantifies motion-based animal behaviour and daily activity in video sequences, allowing automated and non-intrusive surveillance in real-time. The aim of this system is to evaluate significant changes in animals` motion after being experimentally infected with ASF virus. Indeed, pig mobility declined progressively and fell significantly below pre-i… Leer más
Fernandez-Carrion E., Martinez-Aviles M., Ivorra B., Martinez-Lopez B., Ramos AM. y Sanchez-Vizcaino JM..
Short communication: Isolation frequency of bacteria causing lymphadenitis and abscesses in small ruminants in central Spain
Small Ruminant Research 154(2017):5-8.
Infectious lymphadenitis in small ruminants caused by Corynebacterium pseudotuberculosis and Staphylococcus aureus subsp. anaerobius are widely distributed throughout the world, and result in significant economic losses. Trueperella pyogenes has also been associated with lymphadenitis in sheep and goats. In order to determinate the isolation frequency of the different agents associated with lymphadenitis and abscesses, 171 pus samples (135 from sheep and 36 from goats) from 46 flocks were investigated. Isolated bacteria were identified by MALDI-TOF method. S. aureus subsp. anaerobius was the most frequently detected agent. It was identified in 76 animals (59 sheep and 17 goats) from 24 of the surveyed flocks. Of these infected animal, 25 (3… Leer más
de la Fuente R., de las Heras M., Torrijos C., Diez de Tejada P., Perez-Sancho M., Carrion FJ., Orden JA. y Dominguez-Bernal G.
The mobile dso-gene-sso element in rolling circles plasmids of staphylococci reflects the evolutionary history of its resistance gene
Letters In Applied Microbiology 65(3):192-198.
The qacC and lnuA genes of Staphylococcus species were recently proposed to comprise a mobile element when residing on rolling-circle plasmids. Here we present other examples of resistance genes on staphylococcal rolling-circle plasmids, including fosB producing resistance to fosfomycin, cat resulting in resistance to chloramphenicol, and cadB for resistance to the toxic heavy metal cadmium. For three of these genes (qacC, lnuA and fosB) evidence was obtained that the genes have spread between different plasmid backgrounds. The lack of mutations in qacC suggests that the spread occurred relatively recently, while the build up of mutations in lnuA and fosB suggests their mobilization occurred in the more distant past. These observations can … Leer más
Wassenaar TM. y Cabal A.
Application of a specific quantitative real-time PCR (qPCR) to identify Leishmania infantum DNA in spleen, skin and hair samples of wild Leporidae
Veterinary Parasitology 243:92-99.
The aim of this study was to compare a quantitative real-time PCR (qPCR) validated for the detection of Leishmania infantum in dogs with a nested PCR but in wild Leporidae. Additionally, L. infantum results from indirect immunofluorescent antibody test (IFAT) and in vitro culture were also compared with qPCR. Different samples (spleen, skin and hair) recovered from 224 European rabbits and 70 Iberian hares from two green areas of Madrid Council were analysed for the detection of L. infantum. The presence of Leishmania kDNA was detected by qPCR in 58 out of 221 (26.24%), 162 out of 203 (79.8%) and 22 out of 33 (66.67%) analysed rabbits on spleen, skin and hair samples, respectively; and in 7 out of 69 (10.14%), 39 out of 70 (55.71%) and 17 o… Leer más
Ortega-García MV., Moreno I., Dominguez M., de la Cruz ML., Martin AB., Rodriguez-Bertos A., Lopez R., Navarro A., Gonzalez S., Mazariegos M., Goyache J., Dominguez L. y Garcia N..
