Últimas publicaciones científicas SCI
Dynamic Network of Interactions in the Wildlife-Livestock Interface in Mediterranean Spain: An Epidemiological Point of View
Pathogens 9:120(1-16).
The correct management of diseases that are transmitted between wildlife and livestock requires a reliable estimate of the pathogen transmission rate. The calculation of this parameter is a challenge for epidemiologists, since transmission can occur through multiple pathways. The social network analysis is a widely used tool in epidemiology due to its capacity to identify individuals and communities with relevant roles for pathogen transmission. In the present work, we studied the dynamic network of interactions in a complex epidemiological scenario using information from different methodologies. In 2015, nine red deer, seven fallow deer, six wild boar and nine cattle were simultaneously monitored using GPS-GSM-Proximity collars in Doñana N… Leer más
Triguero-Ocana R., Martinez-Lopez B., Vicente J., Barasona JA., Martinez-Guijosa J. y Acevedo P.
A ten-year-surveillance program of zoonotic pathogens in feral pigeons in the City of Madrid (2005-2014): The importance of a systematic pest control
Research In Veterinary Science 128:293-298.
Feral pigeons have increased in urban settings worldwide becoming a potential health risk for humans and other animals. Control and surveillance programs are essential to prevent the possible transmission of zoonotic pathogens carried by pigeons. A surveillance program was carried out in Madrid City (Spain) during 2005–2014 to determine the role of urban pigeons as carriers of zoonotic agents comparing these results with studies performed elsewhere in the last fifteen years.
A total of 1372 pigeons were randomly captured and tested for detection of Antimicrobial susceptibility and genetic heterogeneity of Campylobacter and Salmonella isolates were determined. During the first phase (August 2005–July 2010), 428 animals were an… Leer más
Perez-Sancho M., Garcia-Seco T., Porrero MC., Garcia N., Gomez S., Camara JM., Dominguez L. y Alvarez J..
Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk
Research In Veterinary Science 128:217-223.
Caprine tuberculosis (TB) is a zoonosis with sanitary and economic repercussions. Caprine TB control programs are based on a test and cull strategy using the intradermal tuberculin tests and slaughterhouse surveillance. However, this approach is not always feasible and may have a limited sensitivity under specific circumstances. In this study, performance of a new experimental test based on the P22 protein complex (P22 ELISA) was evaluated in two TB-infected herds using milk and serum samples and compared with cell-based diagnostic tests. Samples from a low (n = 62, herd 1) and a high (n = 52, herd 2) TB prevalence herd were selected. Moreover, bulk tank
milk samples from both herds were analysed using the P22 ELISA. At the end of th… Leer más
Roy A., Infantes-Lorenzo JA., Dominguez M., Moreno I., Perez-Sancho M., Garcia N., Garcia-Seco T., Alvarez J., Romero B., Gortazar C., de Juan L., Dominguez L. y Bezos J..
Comparison of the sensitivity of laryngeal swabs and deep tracheal catheters for detection of Mycoplasma hyopneumoniae in experimentally and naturally infected pigs early and late after infection
Veterinary Microbiology 241:108500.
Detection of Mycoplasma hyopneumoniae infection in live pigs is a critical component to measure the success of disease control or elimination strategies. However, in vivo diagnosis of M. hyopneumoniae is difficult and the imperfect sensitivity of diagnostic tools has been deemed as one of the main challenges. Here, the sensitivity of laryngeal swabs and deep tracheal catheters for detection of M. hyopneumoniae early and late after infection was determined using inoculation status as a gold standard in experimentally infected pigs and a Bayesian approach in naturally infected pigs. Three-hundred and twenty 8-week old seeder pigs were intra-tracheally inoculated
with M. hyopneumoniae strain 232 and immediately placed with 1920 contact … Leer más
Sponheim A., Alvarez J., Fano E., Schmaling E., Dee S., Hanson D., Wetzell T. y Pieters M.
Prevalence and time trend analysis of antimicrobial resistance in respiratory bacterial pathogens collected from diseased pigs in USA between 2006-2016
Research In Veterinary Science 128:135-144.
Swine respiratory disease complex (SRDC) causes massive economic losses to the swine industry and is a major animal welfare concern. Antimicrobials are mainstay in treatment and control of SRDC. However, there is a lack of data on the prevalence and trends in resistance to antimicrobials in bacterial pathogens associated with SRDC. The objective of this study was to estimate the prevalence and changes in resistance to 13 antimicrobials in swine bacterial pathogens (Streptococcus suis, Pasteurella multocida, Actinobacillus suis and Haemophilus parasuis) in the U.S.A using data collected at University of Minnesota Veterinary Diagnostic Laboratory between 2006 and 2016. For antimicrobials for which breakpoints were available, prevalence of res… Leer más
Hayer SS., Rovira A., Olsen K., Johnson TJ., Vannucci F., Rendahl A., Perez A. y Alvarez J..
Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
Frontiers In Microbiology 10:2990.
Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver-derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from 9 Spanish slaughterhouses (50% National production), that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by a RT-qPCR. The overall seropre… Leer más
Garcia N., Hernandez M., Gutierrez-Boada M., Valero A., Navarro A., Munoz-Chimeno M., Fernandez-Manzano A., Escobar FM., Martinez I., Barcena C., Gonzalez S., Avellon A., Eiros JM., Fongaro G., Dominguez L., Goyache J. y Rodriguez-Lazaro D.
Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration
Cell Metabolism S1550-4131(19)30562-5.
Life on Earth emerged in a hydrogen sulfide (H2S)-rich environment eons ago and with it protein persulfidation mediated by H2S evolved as a signaling mechanism. Protein persulfidation (S-sulfhydration) is a post-translational modification of reactive cysteine residues, which modulate protein structure and/or function. Persulfides are difficult to label and study due to their reactivity and similarity with cysteine. Here, we report a facile strategy for chemoselective persulfide bioconjugation using dimedone-based probes, to achieve highly selective, rapid, and robust persulfide labeling in biological samples with broad utility. Using this method, we show persulfidation is an evolutionarily conserved modification and waves of persulfidation … Leer más
Zivanovic J., Kouroussis E., Kohl JB., Adhikari B., Bursac B., Schott-Roux S., Petrovic D., Miljkovic JL., Thomas-Lopez D., Jung Y., Miler M., Mitchell S., Milosevic V., Gomes JE., Benhar M., Gonzalez-Zorn B., Ivanovic-Burmazovic I., Torregrossa R., Mitchell JR., Whiteman M., Schwarz G., Snyder SH., Paul BD., Carroll KS. y Filipovic MR.
Econometric ARIMA methodology to elucidate the evolution of trends in nosocomial antimicrobial resistance rates in the European Union
International Journal Of Antimicrobial Agents 55(1):105800.
BACKGROUND:
Infectious pathogens harboring cephalosporin or fluoroquinolones resistance constitute a serious hazard to human health.
OBJECTIVES:
The aim of this study is to set up a methodology based on econometric analysis and largest European Union (EU) resistance database (EARS-Net) to model EU nosocomial antimicrobial resistance (AMR) and to detect tendency changes, steps or peaks. The contribution of legislation based on third generation cephalosporin (3GC) and fluoroquinolones (FQ) class referrals on EU resistance rate patterns is evaluated.
METHODS:
We studied 3GC and FQ resistance in nosocomial E. coli, K. pneumoniae and P. aeruginosa in at least 25 out of 30 EU countries (> 94% population coverage… Leer más
Ortega-Huedo R., Cuesta M., Hoefer A. y Gonzalez-Zorn B..
Identifying emerging trends in antimicrobial resistance using Salmonella surveillance data in poultry in Spain
Transboundary And Emerging Diseases 67(1):250-262.
Despite of controls and preventive measures implemented along the food chain, infection with non-typhoidal Salmonella (NTS) remains one of the major causes of foodborne disease worldwide. Poultry is considered one of the major sources of NTS. This has led to the implementation of monitoring and control programs in many countries (including Spain) to ensure that in poultry flocks infection is kept to a minimum and to allow the identification and monitoring of circulating NTS strains and their antimicrobial resistance (AMR) phenotypes. Here, we investigated the information from the monitoring program for AMR in Salmonella from poultry in Spain in 2011-2017 to assess the diversity in phenotypic resistance and to evaluate the program`s ability … Leer más
Alvarez J., Lopez-Perez G., Muellner P., De Frutos C., Ahistrom C., Serrano T., Moreno MA., Duran M., Saez JL., Dominguez L. y Ugarte-Ruiz M..
Hypervitaminosis D has no positive effects on goat tuberculosis and may cause chronic renal lesions
The Veterinary Record 185(24):759.
BACKGROUND:
There is evidence for a link between vitamin D deficiency and active tuberculosis (TB). In human beings, several trials have evaluated the role of vitamin D supplementation in TB treatment with conflicting results. However, the role of vitamin D supplementation in animal TB control has received less attention. The authors evaluated the benefit of vitamin D supplementation for preventing mycobacterial infection or reducing TB lesions (TBL) in a controlled trial with goats naturally exposed to Mycobacterium caprae.
METHODS:
Two groups of goats, a vitamin D-supplemented group and a non-supplemented control group, were housed for 10 months in direct contact with M caprae-infected adult goats. Upon contact … Leer más
Risalde MA., Roy A., Bezos J., Pineda C., Casal C., Diez-Guerrier A., Lopez-Villalba I., Fernandez-Manzano A., Moreno I., de Juan L., Dominguez L. y Gortazar C.
