Últimas publicaciones científicas SCI
National colistin sales versus colistin resistance in Spanish pig production
Research In Veterinary Science 123:141-143.
Colistin resistance was associated with infrequent spontaneous mutations that reduce bacterial fitness until 2015, when the plasmid-mediated colistin resistance gene mcr-1 was discovered in China (Liu et al., 2016). The mcr-1 gene was reported for the first time in Southern Europe in 2016 in nine strains of Escherichia coli and Salmonella enterica from farm animals (poultry and pigs) (Quesada et al., 2016). Currently, eight different mcr genes have been reported (mcr-1 to 8) (Garcia et al., 2018) (Yang et al., 2018) (Wang et al., 2018). Nevertheless, only mcr-1, 3, and 4 have been found in Spain (Hernandez et al., 2017) (Quesada et al., 2016) (Carattoli et al., 2017).
Food-producing animals, particularly pigs, have been shown… Leer más
Miguela-Villoldo P., Hernandez M., Moreno MA., Rodriguez-Lazaro D., Quesada A., Dominguez L. y Ugarte-Ruiz M..
Circulation of plasmids harboring resistance genes to quinolones and/or extended-spectrum cephalosporins in multiple Salmonella enterica serotypes from swine in the United States
Antimicrobial Agents And Chemotherapy 63(4):e02602-18 .
Nontyphoidal Salmonella enterica (NTS) poses a major public-health risk worldwide that is amplified by the existence of antimicrobial resistant strains, especially to quinolones and extended-spectrum cephalosporins (ESC). Little is known on the dissemination of plasmids harboring the acquired genetic determinants that confer resistance to these antimicrobials across NTS serotypes from livestock in the United States.NTS isolates (n=183) from U.S. swine clinical cases retrieved during 2014-2016 were selected for sequencing based on their phenotypic resistance to enrofloxacin (quinolone) or ceftiofur (3rd-generation cephalosporin). De-novo assemblies were used to identify chromosomal mutations and acquired antimicrobial resistance genes (AARGs… Leer más
Elnekave E., Hong SL., Lim S., Hayer SS., Boxrud D., Taylor AJ., Lappi V., Noves N., Johnson TJ., Rovira A., Davies P., Perez A. y Alvarez J..
Immune related genes as markers for monitoring health status of honey bee colonies
BMC Veterinary Research 15(1):72.
Background
Honey bee population decline threatens the beekeeping sector, agriculture and global biodiversity. Early detection of colony mortality may facilitate rapid interventions to contain and prevent mortality spread. Among others, deformed wing virus (DWV) is capable of inducing colony losses, especially when combined with Varroa destructor mite. Since the bee immune system plays a crucial role in ensuring that bees are able to face these pathogens, we explored whether expression of immune genes could serve as biomarkers of colony health.
Results
Herein, we describe a preliminary immunological marker composed of two immune genes (relish and defensin), which provide insight on honey bee antiviral defense mechanism. … Leer más
Barroso-Arevalo S., Vicente-Rubiano M., Puerta F., Molero F. y Sanchez-Vizcaino JM..
Validation of a Real-Time PCR for the Detection of Mycobacterium tuberculosis Complex Members in Bovine Tissue Samples
Frontiers In Veterinary Science 6(61):1-9.
Although the post-mortem diagnosis of bovine tuberculosis is mainly achieved through microbiological culture, the development of other techniques to detect Mycobacterium tuberculosis complex (MTBC) members directly from tissue samples has been pursued. The present study describes the development, optimization and validation of a Real-Time PCR based on the mpb70 gene to detect MTBC members in clinical tissue samples from cattle. Specific primers and a hybridization probe were used to amplify MTBC-specific sequences in order to avoid cross-reaction with non-MTBC species. An Internal Amplification Control (IAC) was included in order to assess the presence of PCR inhibitors in the samples. The PCR was optimized to achieve maximum efficiency, an… Leer más
Lorente-Leal V., Liandris E., Castellanos E., Bezos J., Dominguez L., de Juan L. y Romero B..
Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations
BMC Veterinary Research 15(1):70.
BACKGROUND:
Serum antibody detection has potential as a complementary diagnostic tool in animal tuberculosis (TB) control, particularly in multi-host systems. The objective of the present study was to assess the specificity (Sp) of an enzyme-linked immunosorbent assay (ELISA) based on the new multiprotein complex P22 for the detection of specific antibodies against the Mycobacterium tuberculosis complex (MTC) in the four most relevant domestic animals acting as MTC hosts: cattle, goat, sheep and pig. We used sera from an officially TB-free (OTF) country, Norway, and from a non-OTF one, Spain. The samples included sera from goats that had been vaccinated against M. avium subsp. paratuberculosis (MAP) and sheep from a herd in which Cor… Leer más
Infantes-Lorenzo JA., Moreno I., Roy A., Risalde MA., Balseiro A., de Juan L., Romero B., Bezos J., Puentes E., Akerstedt J., Tessema T., Gortazar C., Dominguez L. y Dominguez M.
Day-old chicks are a source of antimicrobial resistant bacteria for laying hen farms
Veterinary Microbiology 230:221-227.
Antimicrobial resistant bacteria are rarely detected in laying hens and the objective of this longitudinal study was to test day-old chick as a source. Four different commercial batches raised on the same farm were monitored from day-old chick to laying hens using Escherichia coli as a model. Ten colonies from each of the eight samplings per batch were tested for antimicrobial susceptibility using 14 antimicrobials.
Overall (313 isolates), higher resistance percentages were detected for tetracycline (26.8%), followed by sulphonamides (16.3%), ampicillin (16.0 %) and quinolones (10.9% and 9.3% for ciprofloxacin and nalidixic acid, respectively). Resistance percentages of bacteria from day-old chicks were higher than those of pullets a… Leer más
Moreno MA., Garcia-Soto S., Hernandez M., Barcena C., Rodriguez-Lazaro D., Ugarte-Ruiz M. y Dominguez L..
Epidemiology and Control of Notifiable Animal Diseases
Frontiers In Veterinary Science 43(6):1-2.
There are a number of criteria by which an animal disease is classified as notifiable; the most important are typically related with its potential to spread internationally, as well as its impact on the health of domestic livestock, wildlife and, not the least, on human health (1). Because of the above, surveillance, early detection, control, and eradication of these diseases is of critical importance for countries in order to maintain or improve their animal health status. This requires the collaboration of all stakeholders involved (e.g., animal health authorities, livestock industry, and veterinary research institutions). The ability to prevent or respond adequately to the novel introduction of a notifiable disease into a herd, region or… Leer más
Salmonella Surveillance Systems in Swine and Humans in Spain: A Review
Veterinary Sciences 6(1):1-16.
Non-typhoid salmonellosis is a common and problematic foodborne zoonotic disease in which pork and pork products can be an important potential source of infection. To prevent this disease, important efforts to monitor the situation in the main source, livestock, are conducted in most developed countries. In the European Union, European Food Safety Agency (EFSA) and European Center for Disease Control (ECDC) compile information at the member-state level, even though important differences in production systems and surveillance systems exist. Here, Salmonella surveillance systems in one of the main sources of foodborne salmonellosis, swine, and humans in Spain were reviewed to identify potential gaps and discuss potential ways of integration u… Leer más
Martinez-Aviles M., Garrido-Estepa M., Alvarez J. y de la Torre A.
Comparison of individual, group and environmental sampling strategies to conduct influenza surveillance in pigs
BMC Veterinary Research 15(1):61.
BACKGROUND:
Influenza A virus (IAV) is an important pathogen in pigs that affects productivity and has important public health implications because of its zoonotic nature. Surveillance is central to the control of influenza, however, detection of IAV infections can be challenging in endemically infected herds with low prevalence of infection.
METHODS:
In groups of suckling (18-21 days of age) and growing (35-45 days of age) pigs, we compared various sampling approaches to detect, isolate and sequence IAV using individual (nasal swabs, nasal wipes and oropharyngeal swabs), group (oral fluids, surface wipes and sow udder skin wipes) and environmental (airborne particles deposited on surfaces and air sa… Leer más
Garrido J., Alvarez J., Culhane M., Nirmala J., Cano JP. y Torremorell M.
A new test to detect antibodies against Mycobacterium tuberculosis complex in red deer serum
The Veterinary Journal 15(1):70.
Red deer (Cervus elaphus) farming is a growing economic activity worldwide. However, the capacity of this species to act as reservoir of animal tuberculosis (TB) poses a threat to other wildlife and to livestock. Diagnostic assay accuracy in this species is therefore highly relevant for prevention and control measures. Our aim was to evaluate the diagnostic performance of the protein complex P22, obtained from Mycobacterium bovis derived purified protein derivative (bPPD), as a candidate antigen for the detection of antibodies against Mycobacterium tuberculosis complex (MTC). We assessed the performance of this new antigen in indirect enzyme-linked immunosorbent assays (ELISA) in TB-positive and TB-negative red deer, in comparison with a bP… Leer más
Thomas J., Infantes-Lorenzo JA., Moreno I., Romero B., Garrido F., Juste R., Dominguez M., Dominguez L., Gortazar C. y Risalde MA.
