Últimas publicaciones científicas SCI
Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis
Veterinary Research 50:82.
Effective vaccines against tuberculosis (TB) are needed in order to prevent TB transmission in human and animal populations. Evaluation of TB vaccines may be facilitated by using reliable animal models that mimic host pathophysiology and natural transmission of the disease as closely as possible. In this study, we evaluated the immunogenicity and efficacy of two attenuated vaccines, BCG and MTBVAC, after each was given to 17 goats (2 months old) and then exposed for 9 months to goats infected with M. caprae. In general, MTBVAC-vaccinated goats showed higher interferon-gamma release than BCG vaccinated goats in response to bovine protein purified derivative and ESAT-6/CFP-10 antigens and the response was significantly higher than that observ… Leer más
Roy A., Tome-Sanchez I., Romero B., Lorente-Leal V., Infantes-Lorenzo JA., Dominguez M., Martin C., Aguilo N., Puentes E., Rodriguez E., de Juan L., Risalde MA., Gortazar C., Dominguez L. y Bezos J..
Risk of African swine fever virus introduction into the United States through smuggling of pork in air passenger luggage
Scientific Reports 9(1):14423.
African swine fever causes substantial economic losses in the swine industry in affected countries. Traditionally confined to Africa with only occasional incursions into other regions, ASF began spreading into Caucasian countries and Eastern Europe in 2007, followed by Western Europe and Asia in 2018. Such a dramatic change in the global epidemiology of ASF has resulted in concerns that the disease may continue to spread into disease-free regions such as the US. In this study, we estimated the risk of introduction of ASF virus into the US through smuggling of pork in air passenger luggage. Results suggest that the mean risk of ASFV introduction into the US via this route has increased by 183.33% from the risk estimated before the disease ha… Leer más
Jurado C., Mur L., Perez-Aguirreburualde MS., Cadenas-Fernández E., Martinez-Lopez B., Sanchez-Vizcaino JM. y Perez A.
Tuberculosis vaccination sequence effect on protection in wild boar
Comparative Immunology, Microbiology And Infectious Diseases 66:101329.
The Eurasian wild boar (Sus scrofa) is a reservoir for tuberculosis (TB) in which vaccination is a valuable tool for control. We evaluated the protection and immune response achieved by homologous and heterologous regimes administering BCG and heat-inactivated Mycobacterium bovis (IV). Twenty-one wild boar piglets were randomly allocated in five groups: Control, homologous BCG, homologous IV, heterologous IV-BCG, heterologous BCG-IV. Significant 67% and 66% total lesion score reductions were detected in homologous IV (IVx2) and heterologous IV-BCG groups when compared with Control group (F4,16 = 6.393, p = 0.003; Bonferroni Control vs IVx2 p = 0.026, Tukey Control vs IV-BCG p = 0.021). No sign… Leer más
Diez-Delgado I., Sevilla IA., Garrido JM., Romero B., Geijo MV., Dominguez L., Juste RA., Aranaz A., de la Fuente J. y Gortazar C.
Mapping changes in the spatiotemporal distribution of lumpy skin disease virus
Transboundary And Emerging Diseases 66(5):2045-2057.
Lumpy skin disease virus (LSDV) is an infectious disease of cattle transmitted by arthropod vectors which results in substantial economic losses due to impact on production efficiency and profitability, and represents an emerging threat to international trade of livestock products and live animals. Since 2015, the disease has spread into the Northern Hemisphere including Azerbaijan, Kazakhstan, the Russian Federation and the Balkans. The rapid expansion of LSDV in those regions represented the emergence of the virus in more temperate regions than those in which LSDV traditionally occurred. The goal of this study was to assess the risk for further LSDV spread through the a) analysis of environmental factors conducive for LSDV, and b) estimat… Leer más
Machado G., Korennoy FI., Alvarez J., Picasso C., Perez A. y VanderWaal K.
Emergent subtype of hepatitis E virus genotype 3 in wild boar in Spain
Transboundary And Emerging Diseases 66(5):1803-1808.
Wild boar (Sus scrofa) is considered the main wildlife reservoir of zoonotic hepatitis E virus (HEV) genotypes. The aim of this study was to evaluate the circulation of HEV in free-ranging wild boar in the Doñana National Park (DNP), Spain. Blood samples were collected from 99 wild boar in the DNP during 2015. Sera were analyzed in parallel using indirect ELISA and real-time RT-PCR. A total of 57 of the 99 tested animals (57.6%; 95%CI: 47.8-67.3%) had anti-HEV antibodies, indicating that this virus is widespread in wild boar in the DNP. HEV RNA was detected in one animal and phylogenetic analysis showed that the sequence isolated belonged to subtype 3r. The results suggest a potential risk of zoonotic transmission of this novel HEV-3 subtyp… Leer más
Caballero-Gomez J., Jiménez-Ruiz S., Lopez-Lopez P., Vicente J., Risalde MA., Cano-Terriza D., Frias M., Barasona JA., Rivero A., Garcia-Bocanegra I. y Rivero-Juarez A.
Carbapenemase-producing Elizabethkingia meningoseptica from healthy pigs associated with colistin use in Spain
Antibiotics 8,146.
Carbapenems are considered last-resort antimicrobials, especially for treating infections involving multidrug-resistant Gram-negative bacteria. In recent years, extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Gram-negative bacteria have become widespread in hospitals, community settings, and the environment, reducing the range of effective therapeutic alternatives. The use of colistin to treat infection caused by these multi-drug bacteria may favour the selection and persistence of carbapenem-resistant bacteria. In this study, it is described, for the first time to our knowledge, a carbapenemase-producing isolate of Elizabethkingia meningoseptica from healthy pigs in Spain. The isolate we report was recovered during a … Leer más
Miguela-Villoldo P., Hernandez M., Moreno MA., Rodriguez-Lazaro D., Quesada A., Dominguez L. y Ugarte-Ruiz M..
Risk of Introduction of Infectious Animal Diseases for Europe Based on the Health Situation of North Africa and the Arabian Peninsula
Frontiers In Veterinary Science 293(6):1-13.
The current growth of the human population, the intensification of animal production, climate change or globalization favors an increase in the transmission of infectious diseases. Risk analysis is the tool that allows the identification of the factors involved in the introduction and the spread of infectious diseases. The main objective of this work is to evaluate the risk of entry of animal infectious zoonotic and non-zoonotic diseases from North Africa and the Arabian Peninsula to countries of the European Union. A probabilistic formulation has been developed to obtain the probabilities of introduction of diseases associated with each possible route of entry in the European Union. The results show that, among the infectious diseases anal… Leer más
Masso-Sagues E., Fernandez-Carrion E. y Sanchez-Vizcaino JM..
Prevalence of Beta-Lactam and Quinolone/Fluoroquinolone Resistance in Enterobacteriaceae From Dogs in France and Spain-Characterization of ESBL/pAmpC Isolates, Genes, and Conjugative Plasmids
Frontiers In Veterinary Science 279(6):1-10.
Quantitative data on fecal shedding of antimicrobial-resistant bacteria are crucial to assess the risk of transmission from dogs to humans. Our first objective was to investigate the prevalence of quinolone/fluoroquinolone-resistant and beta-lactam-resistant Enterobacteriaceae in dogs in France and Spain. Due to the particular concern about possible transmission of extended-spectrum cephalosporin (ESC)-resistant isolates from dogs to their owners, we characterized the ESBL/pAmpC producers collected from dogs. Rectal swabs from 188 dogs, without signs of diarrhea and that had not received antimicrobials for 4 weeks before the study, were quantified for total and resistant Enterobacteriaceae on selective media alone or containing relevant ant… Leer más
Dupouy V., Abdelli M., Moyano G., Arpailange N., Bibbal D., Cadiergues MC., Lopez-Pulin D., Sayah-Jeanne S., de Gunzburg J., Saint-Lu N., Gonzalez-Zorn B., Andremont A. y Bousquet-Melou A.
Modeling the Accuracy of Two in-vitro Bovine Tuberculosis Tests Using a Bayesian Approach
Frontiers In Veterinary Science 6:261.
Accuracy of new or alternative diagnostic tests is typically estimated in relation to a well-standardized reference test referred to as a gold standard. However, for bovine tuberculosis (bTB), a chronic disease of cattle, affecting animal and public health, no reliable gold standard is available. In this context, latent-class models implemented using a Bayesian approach can help to assess the accuracy of diagnostic tests incorporating previous knowledge on test performance and disease prevalence. In Uruguay, bTB-prevalence has increased in the past decades partially because of the limited accuracy of the diagnostic strategy in place, based on intradermal testing (caudal fold test, CFT, for screening and comparative cervical test, CCT, for c… Leer más
Picasso C., Perez A., Gil A., Nunez A., Salaberry X., Suanses A. y Alvarez J..
The bla NDM-1 carrying IncA/C2 plasmid underlies structural alterations and co-integrate formation in vivo
Antimicrobial Agents And Chemotherapy 63(8).
In 2012, a carbapenemase-producing Salmonella (S.) Corvallis carrying a bla NDM-1 multi-resistance IncA/C2 plasmid, apart from IncHI2 and ColE-like plasmids, was detected in a wild bird in Germany. In recent broiler chicken infection study, we observed transfer of this bla NDM-1-carrying IncA/C2 plasmid to other Enterobacteriaceae Here, we focused on the stability of this plasmid and gained insight into type and frequency of its structural alterations after an in vivo passage in a broiler infection study… Leer más
Hadziabdic S., Fischer J., Borowiak M., Malorny B., Juraschek K., Kasbohrer A., Guerra B., Deneke C., Gonzalez-Zorn B. y Szabo I.
