Últimas publicaciones científicas SCI
Zoonotic Transmission of Diphtheria from Domestic Animal Reservoir, Spain
Emerging Infectious Diseases 28:6.
Toxigenic Corynebacterium ulcerans is as an emerging zoonotic agent of diphtheria. We describe the zoonotic transmission of diphtheria caused by toxigenic C. ulcerans from domestic animals in Spain, confirmed by core-genome multilocus sequence typing. Alongside an increasing number of recent publications, our findings highlight the public health threat posed by diphtheria reemergence.
Diphtheria has been increasing in relevance because of increasing individual travel and surges in mass relocation events of refugees, asylum seekers, and immigrants from countries where diphtheria remains endemic (1–3). These importation events, in combination with growing vaccine hesitancy in nonendemic countries, give diphtheria a high potential for r… Leer más
Hoefer A., Herrera-Leon S., Dominguez L., Ordovas-Gavin M., Romero B., Araujo-Piedra XB., Sobrino-Calzada C., Uria-Gonzalez MJ., Herrera-Leon L. y Case Study Investigation Group.
Global Distribution of Extended Spectrum Cephalosporin and Carbapenem Resistance and Associated Resistance Markers in Escherichia coli of Swine Origin - A Systematic Review and Meta-Analysis
Frontiers In Microbiology 13:853810.
Third generation cephalosporins and carbapenems are considered critically important antimicrobials in human medicine. Food animals such as swine can act as reservoirs of antimicrobial resistance (AMR) genes/bacteria resistant to these antimicrobial classes, and potential dissemination of AMR genes or resistant bacteria from pigs to humans is an ongoing public health threat. The objectives of this systematic review and meta-analysis were to: (1) estimate global proportion and animal-level prevalence of swine E. coli phenotypically resistant to third generation cephalosporins (3GCs) and carbapenems at a country level; and (2) measure abundances and global distribution of the genetic mechanisms that confer resistance to these antimicrobial cla… Leer más
Singh-Hayer S., Casanova-Higes A., Paladino E., Elnekave E., Nault A., Johnson T., Bender J., Perez A. y Alvarez J..
Animal abuse in Spanish-breed fighting cocks (Gallus gallus domesticus)
Forensic Science International 2:100050.
Cockfighting is illegal both in the European Union and the United States, although fighting cock husbandry and breed preservation are allowed. Here, we describe the pathological findings observed in the carcasses of two Spanish-breed fighting cocks submitted to our laboratory after an animal abuse-suspected death. The postmortem examination revealed these cocks may have been used for cockfighting (comb, ear lobes, wattles, and chest feather removal; and trimmed spurs). The severe extensive hemorrhages observed in the cervical region of both birds, affecting C1-C4 in bird No. 1 and C8-C11 in No. 2, suggested cervical dislocation in a non-professional manner. To the best of our knowledge, this is the first report of abuse in a Spanish-breed f… Leer más
Rebollada A., Barcena C., Barreno-San Antolin L., Pizarro M., Dominguez L. y Rodriguez-Bertos A..
Garvicins AG1 and AG2: Two Novel Class IId Bacteriocins of Lactococcus garvieae Lg-Granada
International Journal Of Molecualr Sciences 23(9):4685.
Lactococcus garvieae causes infectious diseases in animals and is considered an emerging zoonotic pathogen involved in human clinical conditions. In silico analysis of plasmid pLG50 of L. garvieae Lg-Granada, an isolate from a patient with endocarditis, revealed the presence of two gene clusters (orf46-47 and orf48-49), each one encoding a novel putative bacteriocin, i.e., garvicin AG1 (GarAG1; orf46) and garvicin AG2 (GarAG2; orf48), and their corresponding immunity proteins (orf47 and orf49). The chemically synthesised bacteriocins GarAG1 and GarAG2 presented inhibitory activity against pathogenic L. garvieae strains, with AG2 also being active against Listeria monocytogenes, Listeria ivanovii and Enterococcus faecalis. Genetic organisati… Leer más
Maldonado-Barragan A., Alegria-Carrasco E., Blanco MM., Vela AI., Fernandez-Garayzabal JF., Rodriguez JM. y Gibello A.
Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs
Veterinary Research 53(1):31.
Trained immunity is the capacity of innate immune cells to produce an improved response against a secondary infection after a previous unrelated infection. Salmonellosis represents a public health issue and affects the pig farming industry. In general, vaccination against salmonellosis is still facing problems regarding the control of distinct serovars. Therefore, we hypothesized that an immunostimulant based on heat inactivated Mycobacterium bovis (HIMB) could have an immune training effect in pigs challenged with Salmonella enterica serovar Choleraesuis (S. Choleraesuis) and decided to explore the amplitude of this non-specific immune response. For this purpose, twenty-four 10 days-old female piglets were randomly separated in three group… Leer más
Vaz-Rodrigues R., Ferreras-Colino E., Ugarte-Ruiz M., Pesciarioli M., Thomas J., Garcia-Seco T., Sevilla IA., Perez-Sancho M., Mateo R., Dominguez L., Gortazar C. y Risalde MA.
Potential for improved detection of bovine tuberculosis by targeting combined blood biomarkers in multi-test algorithms
Veterinary Immunology And Immunopathology 248:110419.
Bovine tuberculosis (bTB) control programs can be improved by combined use of tests for humoral and cell-mediated immune responses targeting multiple biomarkers of Mycobacterium bovis. To further the diagnostic benefits of this approach, we used Dual Path Platform (DPP) technology to test sera from cattle with naturally acquired bTB in the United States (US) and Spain for the presence of M. bovis antigen, IgM and/or IgG antibodies to MPB70/MPB83 fusion antigen in conjunction with tuberculin skin tests (TST) or interferon-gamma release assays (IGRA). When TST was complemented with detection of IgM and IgG antibodies, the diagnostic sensitivity increased from 85.4% to 95.1% in the US and from 64.2% to 81.5% in Spain. Likewise, adding the DPP … Leer más
Sridhara AA., Johnathan-Lee A., Elahi R., Sikar-Gang A., Lambotte P., Esfandiari J., de Juan L., Gortazar C., Marpe BN., Thacker TC., Palmer MV., Waters WR. y Lyashchenko K.
First Detection of SARS-CoV-2 B.1.617.2 (Delta) Variant of Concern in a Symptomatic Cat in Spain
Frontiers In Veterinary Science 9:841430.
Natural and experimental SARS-CoV-2 infection in pets has been widely evidenced since the beginning of the COVID-19 pandemic. Among the numerous affected animals, cats are one of the most susceptible species. However, little is known about viral pathogenicity and transmissibility in the case of variants of concern (VOCs) in animal hosts, such as the B.1.617.2 (Delta) variant first detected in India. Here, we have identified the B.1.617.2 (Delta) VOC in a cat living with a COVID-19 positive owner. The animal presented mild symptoms (sneezing) and a high viral load was detected in the oropharyngeal swab, suggesting that an active infection was occurring in the upper respiratory tract of the cat. Transmission from the owner to the cat occurred… Leer más
Barroso-Arevalo S., Sánchez-Morales L., Perez-Sancho M., Dominguez L. y Sanchez-Vizcaino JM..
Bcl-2 Immunoexpression in Feline Epitheliotropic Intestinal T-Cell Lymphomas
Veterinary Sciences 9:168.
Abstract: Lymphoma is the most common malignant hematopoietic neoplasm in domestic felines. Twenty-two cases of feline epitheliotropic duodenal T-cell lymphoma were characterized morphologically and immunohistochemically (CD3, Pax5, Ki-67), and Bcl-2 immunoexpression was established. Most cases were in domestic shorthair cats (88.2%), with a mean age of 11.2 years. All lymphomas were CD3+, with a low-to-moderate expression of Ki-67 (< 30%). A correlation between the tumoral pattern of infiltration in the lamina propria and the intraepithelial distribution of the neoplastic lymphocytes was established (p = 0.0155). Intraepithelial nests of neoplastic lymphocytes were predominantly observed in lymphomas with a patchy distribution in the lamin… Leer más
Rebollada A., Porras-Gonzalez N., Calvo-Ibbitson A., Rodriguez-Franco F. y Rodriguez-Bertos A..
The Evolution of African Swine Fever in China: A Global Threat?
Frontiers In Veterinary Science 9:828498.
African swine fever (ASF) is one of the most critical diseases in the pig industry. In Asia, 15 countries have already reported an outbreak as of November 22, 2021. In 2021, China reported the genotype II lower virulent ASF virus (ASFV) and the emergence of genotype I ASFV. ASF is generally known as a contagious and lethal disease, but if chronic infection spreads, then disease control would be more difficult. In the current study, we highlighted the possibility of lower virulent virus distribution throughout China and the subsequent general risk of the virus being released from the country. The kernel density estimation showed that the two highest kernel density areas of ASF notification were located in Northeast and Midwest China. Four of… Leer más
Ito S., Bosch J., Martinez-Aviles M. y Sanchez-Vizcaino JM..
Molecular detection of Tritrichomonas foetus in bovine samples: a novel real-time polymerase chain reaction (PCR) assay targeting EF1-alpha-Tf1 and a comparative study of published PCR techniques
Parasitology Research 00.
The parasite T. foetus causes trichomonosis in cattle but is generally asymptomatic in males. Thus, many bulls carrying the disease go unnoticed, making the detection of T. foetus in bulls an important aspect for its control. Due to drawbacks posed by its cultivation, PCR is a preferred option for diagnostic laboratories. Most published PCR protocols target the genomic region compring the 18S, 5.8S, and 28S rRNA genes and internal transcribed spacers 1 and 2 (rRNA-ITS region), homologous to that of other Tritrichomonas species. There is minimal information on alternative genetic targets and no comparative studies have been published. We compared a protocol based on the microsatellite TfRE (called H94) and five protocols based on the rRNA-IT… Leer más
Polo C., Garcia-Seco T., Fernandez V., Hernandez M., Briones V., Diez-Guerrier A., Dominguez L. y Perez-Sancho M..
