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Últimas publicaciones científicas ISI

1 de diciembre de 2017

Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain

Emerging Infectious Diseases 23(12):2013-2016.

     Streptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associated with different clinical backgrounds. We also investigated erythromycin resistance mechanisms and phenotypes and virulence genes. We observed 2 emm types: emm12 (11 isolates) and emm77 (4 isolates). Similarly, we observed 2 genetic linages, sequence type (ST) 26 and ST63. Most isolates exhibited the M macrolide resistance phenotype and the mefA/ermB genotype. Isolates were grouped into 2 clones on the basis of emm-MLST-PFGE-virulence gene pro… Leer más

Vela AI., Villalon P., Saez-Nieto JA., Chacon G., Dominguez L. y Fernandez-Garayzabal JF..

1 de diciembre de 2017

Multi-Laboratory Evaluation of a Novel Lateral Flow Immunochromatographic Assay for Confirming Isolation of Mycobacterium bovis from Veterinary Diagnostic Specimens

Journal Of Clinical Microbiology 55(12):3411-3425.

     A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for Mycobacterium bovis and M. caprae cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotyping or qPCR) in each laboratory; liquid (MGIT or BacT/Alert) and/or solid (Stonebrink, Coletsos or Lowenstein-Jensen) cultures were tested. In comparison to spoligotyping of acid-fast positive MGIT cultures, percentage agreement between positive LFD and spoligotyping results was excellent in two UK laboratories (97.7-100%), but lower in the Spanish context (76%) where spoligotyping was applied to MGIT cultures previously confirm… Leer más

Stewart LD., McCallan L., McNair J., McGoldrick A., Morris R., Moyen JL., de Juan L., Romero B., Alonso E., Parsons SDC., van Helden P., Araujo FR. y Grant IR.

1 de diciembre de 2017

Differentiation of Flavobacterium psychrophilum from Flavobacterium psychrophilum-like species by MALDI-TOF mass spectrometry

Research In Veterinary Science 115(2017):345-352.

     Rainbow trout fry syndrome (RTFS) is an important infectious disease caused by Flavobacterium psychrophilum affecting farmed salmonids worldwide. Other Flavobacterium psychrophilum-like species (F. plurextorum, F. oncorhynchi, F. tructae, F. collinsii and F. piscis) have been isolated from diseased rainbow trout fry suspected of RTFS although the epidemiological and clinical relevance of these pathogens are unknown. The objective of this study was to evaluate the potential use of MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time of Flight) Mass Spectrometry as method for specific identification of F. psychrophilum and its differentiation from other F. psychrophilum-like species isolated from diseased fish. Fifty-three isolates wer… Leer más

Perez-Sancho M., Vela AI., Wiklund T., Kostrzewa M., Dominguez L. y Fernandez-Garayzabal JF..

15 de noviembre de 2017

Genome comparison of erythromycin resistant Campylobacter from turkey identifies hosts and pathways for horizontal spread of erm(B) genes

Frontiers In Microbiology 2240(8):1-8.

     Pathogens in the genus Campylobacter are the most common cause of food-borne bacterial gastro-enteritis. Campylobacteriosis, caused principally by Campylobacter jejuni and C. coli, is transmitted to humans by food of animal origin, especially poultry. As for many pathogens, antimicrobial resistance in Campylobacter is increasing at an alarming rate. Erythromycin prescription is the treatment of choice for clinical cases requiring antimicrobial therapy but this is compromised by mobility of the erythromycin resistance gene erm(B) between strains. Here we evaluate resistance to six antimicrobials in 170 Campylobacter isolates (133 C. coli and 37 C. jejuni) from turkeys. Erythromycin resistant isolates (n=85; 81 C. coli and 4 C. jejuni) were s… Leer más

Florez-Cuadrado D., Ugarte-Ruiz M., Meric G., Quesada A., Porrero MC., Pascoe B., Saez Llorente JL., Lopez-Orozco G., Dominguez L. y Sheppard S.

10 de noviembre de 2017

First analysis by MALDI-TOF MS technique of Chryseobacterium species relevant

Journal Of Fish Diseases 2017:1-5.

Perez-Sancho M., Vela AI., Kostrzewa M., Zamora L., Casamayor A., Dominguez L. y Fernandez-Garayzabal JF..

2 de noviembre de 2017

Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis

Clinical Proteomics (2017):1-10.

     Bovine purified protein derivative (bPPD) and avian purified protein derivative (aPPD) are widely used for bovine tuberculosis diagnosis. However, little is known about their qualitative and quantitative characteristics, which makes their standardisation difficult. In addition, bPPD can give false-positive tuberculosis results because of sequence homology between Mycobacterium bovis (M. bovis) and M. avium proteins. Thus, the objective of this study was to carry out a proteomic characterisation of bPPD, aPPD and an immunopurified subcomplex from bPPD called P22 in order to identify proteins contributing to cross-reactivity among these three products in tuberculosis diagnosis… Leer más

Infantes-Lorenzo JA., Moreno I., Risalde MA., Roy A., Villar M., Romero B., Ibarrola N., de la Fuente J., Puentes E., de Juan L., Gortazar C., Bezos J., Dominguez L. y Dominguez M.

1 de noviembre de 2017

Development and evaluation of an interferon gamma assay for the diagnosis of tuberculosis in red deer experimentally infected with Mycobacterium bovis

BMC Veterinary Research 16;13(1):341.

Red deer (Cervus elaphus) is regarded as an epidemiologically relevant host for Mycobacterium bovis (M. bovis) and closely related members of the Mycobacterium tuberculosis complex that cause animal tuberculosis (TB). The standard antemortem screening test for the detection of TB in deer is the intradermal tuberculin skin test, but the detection of interferon-gamma (IFNγ) produced by white blood cells exposed to M. bovis antigens can be used as an alternative or supplemental assay in most TB eradication/control programs. This study aims to develop an in-house sandwich ELISA for deer IFNγ, based on the cross-reactivity of the antibodies to both cervid and bovine IFNγ, and to evaluate the potential of this as… Leer más

Risalde MA., Thomas J., Sevilla I., Serrano M., Ortiz JA., Garrido J., Dominguez M., Dominguez L., Gortazar C. y Ruiz-Fons JF.

1 de noviembre de 2017

Streptococcus ovuberis sp. nov., isolated from a subcutaneous abscess in the udder of a sheep

International Journal Of Systematic And Evolutionary Microbiology 2017(67):4340-4344.

     One unidentified, Gram-stain-positive, catalase-negative coccus-shaped organism was recovered from a subcutaneous abscess of the udder of a sheep and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolate was tentatively assigned to the genus Streptococcus, although the organism did not appear to match any recognized species. 16S rRNA gene sequence comparison studies confirmed its identification as a member of the genus Streptococcus and showed that the nearest phylogenetic relatives of the unknown coccus corresponded to Streptococcus moroccensis and Streptococcus cameli (95.9 % 16S rRNA gene sequence similarity). The sodA sequence analysis showed less than 89.3 % sequenc… Leer más

Zamora L., Perez-Sancho M., Fernandez-Garayzabal JF., Orden JA., Dominguez-Bernal G., de la Fuente R., Dominguez L. y Vela AI..

1 de octubre de 2017

Body temperature and motion: Evaluation of an online monitoring system in pigs challenged with Porcine Reproductive & Respiratory Syndrome Virus

Research In Veterinary Science 114(2017):482-488.

     Highly contagious and emerging diseases cause significant losses in the pig producing industry worldwide. Rapid and exact acquisition of real-time data, like body temperature and animal movement from the production facilities would enable early disease detection and facilitate adequate response. In this study, carried out within the European Union research project RAPIDIA FIELD, we tested an online monitoring system on pigs experimentally infected with the East European subtype 3 Porcine Reproductive & Respiratory Syndrome Virus (PRRSV) strain Lena. We linked data from different body temperature measurement methods and the real-time movement of the pigs. The results showed a negative correlation between body temperature and movement of the … Leer más

Suli T., Halas M., Benyeda Z., Boda R., Belak S., Martinez-Aviles M., Fernandez-Carrion E. y Sanchez-Vizcaino JM..

1 de octubre de 2017

MCR-2-mediated plasmid-borne polymyxin resistance most likely originates from Moraxella pluranimalium

The Journal Of Antimicrobial Chemotherapy 72(10):2947-2949.

     The recent identification of the first plasmid-mediated polymyxin resistance determinants, namely the MCR-1 and MCR-2 enzymes, has constituted an ultimate threat of pandrug resistance in Gram-negative organisms.1–3 These enzymes have been reported only in Enterobacteriaceae, mainly in Escherichia coli and Klebsiella pneumoniae; however, there have been reports from other enterobacterial genera, including Enterobacter, Salmonella and Shigella.1 The mcr-1 and mcr-2 genes have both been identified from cattle and pigs.1–3 In addition, the mcr-1 gene has been identified from chickens,4 but also from river samples and vegetables… Leer más

Poirel L., Kieffer N., Fernandez-Garayzabal JF., Vela AI., Larpin Y. y Nordmann P.

Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ICM 186 SUAT 79 SUAT 13
2 ZTA 179 ZTA 78 MYC 12
3 MYC 158 MYC 63 ZTA 11
4 SUAT 146 ICM 42 ICM 11
  VISAVET 729 284 52

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.45 ZTA 3.477 ZTA 3.769
2 MYC 2.606 ICM 2.488 ICM 2.75
3 SUAT 2.449 SUAT 2.406 SEVISEQ 2.442
4 ICM 2.285 SEVISEQ 2.251 SUAT 2.38
5 SEVISEQ 2.251 SAP 2.236 MYC 2.32
VISAVET 2.621 2.616 2.712

ISI Scientific Publications
Total Last 60 mo.
1 Vet. Microbiol. 59 Vet. Microbiol. 25
2 J. Clin. Microbiol. 38 Transbound. Emerg. Dis. 24
3 Transbound. Emerg. Dis. 35 Res. Vet. Sci. 21
4 Int. J. Syst. Evol. Microbiol. 31 PLoS ONE 17
5 Res. Vet. Sci. 30 BMC Vet. Res 14
  All journals 207   All journals 33