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Últimas publicaciones científicas SCI

17 de julio de 2025

Global dissemination of npmA mediated pan-aminoglycoside resistance via a mobile genetic element in Gram-positive bacteria

Nature Communications 16(1):6360.

     The npmA gene, encoding a 16S rRNA methyltransferase, confers resistance to all clinically available aminoglycosides, posing a significant threat to effective antibiotic therapy. We analyze 1,932,812 bacterial genomes to investigate the distribution and mobilization of npmA variants. npmA is not found in Gram-negative bacteria, where it was originally described, but is identified among Gram-positive bacteria, predominantly as the npmA2 variant in the globally distributed Clostridioides difficile ST11 lineage. We also detect npmA2 in two vancomycin-resistant Enterococcus faecium isolates from a Dutch hospital. Upon sequencing and phenotypic analysis, we determine that E. faecium isolates are pan-resistant to aminoglycosides. Genomic characte… Leer más

Serna-Bernaldo C., Matamoros BR., Pulido-Vadillo M., Delgado-Blas JF., Jansen RR., Willems RJL., Almeida A., Harrison EM., Dupuy B., Coll F. y Gonzalez-Zorn B..

1 de julio de 2025

Spatiotemporal Distribution and Clinical Characteristics of Zoonotic Tuberculosis, Spain, 2018-2022

Emerging Infectious Diseases 31(7):1344-1352.

     Zoonotic tuberculosis (zTB) is a communicable disease that has major effects on both human and animal health. Spain reports the highest number of zTB cases in humans annually in the European Union. We describe the epidemiology of human cases of zTB caused by Mycobacterium bovis and M. caprae in Spain during 2018-2022. The incidence of M. bovis infection compared with M. tuberculosis infection was higher in patients who were native-born (adjusted odds ratio [aOR) 2.32, 95% CI 1.44-3.82), HIV-negative (aOR 3.39, 95% CI 1.24-14.0), or had extrapulmonary forms of TB (aOR 2.20, 95% CI 1.46-3.28). The spatial pattern differed by M. tuberculosis complex species; we identified 3 significant clusters of M. bovis and 1 of M. caprae in bovine TB-free … Leer más

Roy A., Gomez-Barroso D., Cruz-Ferro E., Fernandez A., Martinez-Pino I., del Henar-Marcos M., Ursua-Díaz I., Miras S., Echave N., Ouranou E., Romero B., Herrera-Leon L., Herrador Z. y Study Group on Zoonotic Tuberculosis.

27 de mayo de 2025

Sponge-based environmental DNA detection as a useful tool in monitoring Mycobacterium tuberculosis complex markers in European bison (Bison bonasus)

Scientific Reports 15(1):18503.

     The European bison (Bison bonasus), also called wisent, is the largest terrestrial mammal in Europe, classified by the International Union for Conservation of Nature (IUCN) as a "Near Threatened" species. Tuberculosis (TB) represents a well-known threat to wisent, especially nowadays when infectious diseases are emerging to this species, due to locally high population density and frequent translocation (and consequently increased exposure to infectious diseases). There is an urgent need to control the TB-epidemiological situation in the European bison environment. This study aimed to assess the usefulness of the sponge-based environmental-DNA (eDNA) for monitoring TB in free-ranging and captive European bison herds based on the knowledge of… Leer más

Didkowska A., Perez-Sancho M., Herranz-Benito C., Klich D., Anusz K., Witkowski L., Dominguez L. y Gortazar C.

8 de mayo de 2025

Mobile integrons encode phage defense systems

Science 388:6747.

     Integrons are bacterial genetic elements that capture, stockpile, and modulate the expression of genes encoded in integron cassettes. Mobile integrons (MIs) are borne on plasmids, acting as a vehicle for hundreds of antimicrobial resistance genes among key pathogens. These elements also carry gene cassettes of unknown function (gcus) whose role and adaptive value remain unexplored. In this work, we show that gcus encode phage resistance systems, many of which are newly discovered. Bacteriophage resistance integron cassettes (BRiCs) can be combined and mixed with resistance cassettes to produce multiphage or drug and phage resistance. The fitness costs of BRiCs are variable and dependent on the genetic context and can be modulated by changin… Leer más

Kieffer N., Hipolito A., Ortiz-Miravalles L., Blanco P., Delobelle T., Vizuete P., Ojeda F., Jove T., Jurenas D., García-Quintanilla M., Carvalho A., Domingo-Calap P. y Escudero JA..

1 de mayo de 2025

Evaluation of techniques for post-mortem diagnosis of Mycobacterium tuberculosis complex infection in goats

Veterinary Microbiology 304:110485.

     Goats are highly susceptible to Mycobacterium tuberculosis complex (MTBC) infection and can play an important role in the epidemiology of animal tuberculosis (TB) in certain epidemiological scenarios. Historically, culture has been considered the only gold standard technique for post-mortem confirmation of MTBC infection. However, it is constrained by its low sensitivity, the slow growth of MTBC, and stringent biosecurity requirements. Thus, alternative post-mortem techniques are essential for effective TB control and eradication. This study aimed to compare the use of different diagnosis techniques for post-mortem confirmation of MTBC infection in goats positive for official tuberculin skin tests (TST). The techniques evaluated were direct… Leer más

Jimenez-Martin D., Munoz-Fernandez L., Agullo-Ros I., Jimenez-Pizarro N., Cano-Terriza D., Garcia-Bocanegra I., Romero B., Caballero-Gomez J., Gonzalvez M., Rejon S., Martinez R. y Risalde MA.

1 de mayo de 2025

Reproductive loss attributed to Lactococcus petauri infection in a black-and-white ruffed lemur

Journal Of Veterinary Diagnostic Investigation 37(3):471-474.

     Lactococci have been associated with fetal and neonatal infections in humans and cattle. Here we describe a case of reproductive loss attributed to Lactococcus petauri in a lemur. A full-term black-and-white ruffed lemur (Varecia variegata) was found dead in the indoor area of a zoologic exhibit. Classification as a late-term abortion or stillbirth was unclear as the precise gestational time was unknown. A medical checkup of the dam revealed fever and neutrophilic leukocytosis; recovery followed treatment with enrofloxacin. The main histologic findings were placental edema and hemorrhage, hepatic necrosis, desquamated amniotic epithelial cells in alveoli, and subendocardial and myocardial hemorrhages. Tissue Gram stain revealed abundant gra… Leer más

Rebollada A., Vela AI., Canales R., Romani-Cremaschi U., Ugarte-Ruiz M., Buendia A., Perez-Sancho M., Dominguez L., Fernandez-Garayzabal JF. y Rodriguez-Bertos A..

26 de abril de 2025

Trends and Challenges in the Detection and Environmental Surveillance of the Hepatitis E Virus

Microorganisms 13:998.

     The Hepatitis E virus (HEV) is responsible for causing Hepatitis E, a zoonotic disease that has emerged as a significant global health concern, accounting for about 20 million infections and 70,000 deaths annually. Although it is often recognized as a disease that is acute in low-income countries, HEV has also been recognized as a zoonotic disease in high-income countries. The zoonotic transmission requires flexible approaches to effectively monitor the virus, vectors, and reservoirs. However, the environmental monitoring of HEV presents additional challenges due to limitations in current detection methods, making it difficult to accurately assess the global prevalence of the virus. These challenges hinder efforts to fully understand the sc… Leer más

Alves-Elois M., Paula-Pavi C., Ferreira Souza-Hoffmann Jempierre Y., Von Tönnemann-Pilati G., Zanchetta L., Borges-da Silva Grisard H., Garcia N., Rodriguez-Lazaro D. y Fongaro G.

25 de abril de 2025

Effect of oxygen on antimicrobial resistance genes from a one health perspective

Science Of The Total Environment 979:179523.

     Bacteria must face and adapt to a variety of physicochemical conditions in the environment and during infection. A key condition is the concentration of dissolved oxygen, proportional to the partial pressure of oxygen (PO2), which is extremely variable among environmental biogeographical areas and also compartments of the human and animal body. Here, we sought to understand if the phenotype of resistance determinants commonly found in Enterobacterales can be influenced by oxygen pressure. To do so, we have compared the MIC in aerobic and anaerobic conditions of isogenic Escherichia coli strains containing 136 different resistance genes against 8 antibiotic families. Our results show a complex landscape of changes in the performance of resis… Leer más

Ortiz-Miravalles L., Prieto A., Kieffer N., Vergara E., Canton R., San Millan A., Baquero F., Hipolito A. y Escudero JA..

3 de abril de 2025

Revisiting Mycobacterium bovis SB0121 genetic diversity reflects the complexity behind bovine tuberculosis persistence in Spain

Preventive Veterinary Medicine 239:106519.

     Identifying the causes of tuberculosis (TB) chronicity in cattle herds in Spain is a complex endeavour, mainly due to the multiple factors involved in persistence and the clonal population structure of the Mycobacterium tuberculosis complex. This study assessed the genomic diversity among M. bovis isolates belonging to SB0121, the most prevalent genotype in Spain, in chronically-infected herds. A total of 70 M. bovis isolates from 22 herds, located in six Spanish provinces, in which M. bovis SB0121 was isolated in at least three different sampling events were sequenced. Forty-three isolates from wildlife and cattle herds from the same or neighbouring municipalities to the problem herds were also included to identify putative local transmiss… Leer más

Lorente-Leal V., Pozo P., Bezos J., Collado S., Vicente J., Stuber T., Alvarez J., de Juan L. y Romero B..

2 de abril de 2025

Molecular epidemiology of Salmonella Enteritidis in humans and animals in Spain

Antimicrobial Agents And Chemotherapy 69(4):e0073824.

     Salmonella Enteritidis, the most prevalent serovar causing human gastroenteritis, has been traditionally linked to poultry sources. Although antimicrobial resistance (AMR) is not common in this serovar, increasing levels of resistance to fluoroquinolones and ampicillin have been reported in 42 the last years. Here, 298 isolates retrieved from different sources (human, livestock, wildlife, food and
environment) and years (2002-2021) in Spain were analysed to evaluate their diversity, the distribution of AMR-conferring genes (ARGs) and mutations, and reconstruct the epidemiology of infection due to this serovar. Isolates were clustered in two major clades (I, II), with strains in clade I (including 61.5% of all human isolates) display… Leer más

Samper-Cativiela C., Torre-Fuentes L., Bernabé Diéguez B., Maex M., Ugarte-Ruiz M., Carrizo-Coronado P., Hernandez M., Hofle U., Saez-Llorente JL., De Frutos C., Aguero M., Moreno MA., Dominguez L., Herrera-Leon S. y Alvarez J..





Science Publication
Indicators
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ZTA 261 SUAT 60 MYC 12
2 ICM 248 MYC 54 ZTA 11
3 MYC 243 ZTA 54 SUAT 9
4 SUAT 232 ICM 47 ICM 8
5 NED 77 SAP 30 SEVISEQ 5
  VISAVET 1189 307 54

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.919 ZTA 5.228 ZTA 5.4
2 SAP 3.214 SUAT 4.069 MYC 4.508
3 SUAT 2.947 MYC 3.723 SUAT 3.322
4 MYC 2.835 ICM 3.694 NED 3
5 DICM 2.776 SAP 3.248 SEVISEQ 2.98
VISAVET 3.281 4.908 4.940

ISI Scientific Publications
Total Last 60 mo.
1 Transbound Emerg Dis 96 Front Vet Sci 30
2 Vet Microbiol 91 Transbound Emerg Dis 21
3 Prev Vet Med 78 Animals 16
4 Front Vet Sci 68 Sci Rep 13
5 PLoS ONE 60 BMC Vet Res 11
  All journals 359   All journals 39