Últimas publicaciones científicas ISI
The Journal Of Antimicrobial Chemotherapy 72(4):1050-1053.
The mobile colistin resistance gene mcr-1 has been identified worldwide in human and animal sources, while its occurrence in the environment is still largely unknown. The aim of this study was to investigate the presence of mcr-1-harbouring Enterobacteriaceae in water samples obtained from rivers and waste water treatment plants in the area of Barcelona, Spain.
The presence of mcr-1 was detected by PCR. Bacterial identification was performed via MALDI-TOF MS. Resistance to colistin was determined by a broth dilution method. The epidemiological relationship between the positive isolates was assessed with PFGE and ST was determined by MLST. Plasmid characterization was performed by transformation expe… Leer más
Ovejero CM., Delgado-Blas JF., Calero-Caceres W., Muniesa M. y Gonzalez-Zorn B..
Transboundary And Emerging Diseases 64(2):364-373.
Late detection of emergency diseases causes significant economic losses for pig producers and governments. As the first signs of animal infection are usually fever and reduced motion that lead to reduced consumption of water and feed, we developed a novel smart system to monitor body temperature and motion in real time, facilitating the early detection of infectious diseases. In this study, carried out within the framework of the European Union research project Rapidia Field, we tested the smart system on 10 pigs experimentally infected with two doses of an attenuated strain of African swine fever. Biosensors and an accelerometer embedded in an eartag captured data before and after infection, and video cameras were used to monitor the anima… Leer más
Understanding African Swine Fever infection dynamics in Sardinia using a spatially explicit transmission model in domestic pig farms
Transboundary And Emerging Diseases (2017)1-12.
African swine fever virus (ASFV) has been endemic in Sardinia since 1978, resulting in severe losses for local pig producers and creating important problems for the island`s veterinary authorities. This study used a spatially explicit stochastic transmission model followed by two regression models to investigate the dynamics of ASFV spread amongst domestic pig farms, to identify geographic areas at highest risk and determine the role of different susceptible pig populations (registered domestic pigs, non-registered domestic pigs [brado] and wild boar) in ASF occurrence. We simulated transmission within and between farms using an adapted version of the previously described model known as Be-FAST. Results from the model revealed a generally l… Leer más
Deformed wing virus (DWV) in association with Varroa destructor is currently attributed to being responsible for colony collapse in the western honey bee (Apis mellifera). The appearance of deformed individuals within an infested colony has long been associated with colony losses. However, it is unknown why only a fraction of DWV positive bees develop deformed wings. This study concerns two small studies comparing deformed and non-deformed bees. In Brazil, asymptomatic bees (no wing deformity) that had been parasitised by Varroa as pupae had higher DWV loads than non-parasitised bees. However, we found no greater bilateral asymmetry in wing morphology due to DWV titres or parasitisation. As expected, using RT-qPCR, deformed bees were found … Leer más
Brettell LE., Mordecai GJ., Schroeder DC., Jones IM., de Silva JR., Vicente-Rubiano M. y Martin SJ.
Prediction of Pig Trade Movements in Different European Production Systems Using Exponential Random Graph Models
Frontiers In Veterinary Science 4(27):1-12.
In most European countries, data regarding movements of live animals are routinely collected and can greatly aid predictive epidemic modeling. However, the use of complete movements’ dataset to conduct policy-relevant predictions has been so far limited by the massive amount of data that have to be processed (e.g., in intensive commercial systems) or the restricted availability of timely and updated records on animal movements (e.g., in areas where small-scale or extensive production is predominant). The aim of this study was to use exponential random graph models (ERGMs) to reproduce, understand, and predict pig trade networks in different European production systems. Three trade networks were built by aggregating movements of pig batches … Leer más
Relun A., Grosbois V., Alexandrov T., Sanchez-Vizcaino JM., Waret-Szkuta A., Molia S., Charles-Etter EM. y Martinez-Lopez B.
Epidemiology And Infection 145(4):802-817.
Animal health surveillance enables the detection and control of animal diseases including zoonoses. Under the EU-FP7 project RISKSUR, a survey was conducted in 11 EU Member States and Switzerland to describe active surveillance components in 2011 managed by the public or private sector and identify gaps and opportunities. Information was collected about hazard, target population, geographical focus, legal obligation, management, surveillance design, risk-based sampling, and multi-hazard surveillance. Two countries were excluded due to incompleteness of data. Most of the 664 components targeted cattle (26·7%), pigs (17·5%) or poultry (16·0%). The most common surveillance objectives were demonstrating freedom from disease (43·8%) and case det… Leer más
Bisdorff B., Schauer B., Rodriguez-Prieto V., Comin A., Brouwer A ., Dorea F., Drewe J., Hoinville L., Lindberg A., Martinez-Aviles M., Martinez-Lopez B., Peyre M., Pinto Ferreira J., Rushton J., Van Schaik G., Stark KD., Staubach C., Vicente-Rubiano M., Witteveen G., Pfeiffer D. y Hasler B.
CHROMagar mSuperCARBA performance in carbapenem-resistant Enterobacteriaceae isolates characterized at molecular level and routine surveillance rectal swab specimens
Diagnostic Microbiology And Infectious Disease 87(3):207-209.
Performance of the CHROMagar mSuperCARBA media was assessed in both well-characterized carbapenem-resistant Enterobacteriaceae (n=52) and routine surveillance rectal swab specimens (n=211). Limit of detection ranged between 101 and 102CFU/mL except for OXA-48 producers with low-carbapenem MICs (106CFU/mL). High sensitivity (100%) and specificity (100%) were obtained with rectal swabs… Leer más
Garcia-Fernandez S., Hernandez M., Valverde A., Ruiz-Garbajosa P., Morosini MI. y Canton R.
Acta Tropica 169(2017):163-169.
West Nile fever is an emergent disease in Europe. The objective of this study was to conduct a predictive risk mapping of West Nile Virus (WNV) circulation in Spain based on historical data of WNV circulation. Areas of Spain with evidence of WNV circulation were mapped based on data from notifications to the surveillance systems and a literature review. A logistic regression-based spatial model was used to assess the probability of WNV circulation. Data were analyzed at municipality level. Mean temperatures of the period from June to October, presence of wetlands and presence of Special Protection Areas for birds were considered as potential predictors. Two predictors of WNV circulation were identified: higher temperature [adjusted odds rat… Leer más
Evaluation of five serologic assays for bovine tuberculosis surveillance in domestic free-range pigs from southern Spain
Preventive Veterinary Medicine 137(2017):101-104.
In countries where bovine tuberculosis (bTB) is still prevalent the contact among different animal species in extensive systems contributes to the circulation of Mycobacterium bovis (M. bovis) and other members of the Mycobacterium tuberculosis complex (MTC). Thus, free-range pigs can develop subclinical infections and may contribute to disease spread to bovine and wildlife. Serodiagnosis has been proposed as a screening tool for detecting infected pig herds; however, the value of this method to obtain an accurate diagnosis in this species is still not clear. In this study, sensitivity (Se) and specificity (Sp) estimates of four ELISAs and a lateral flow immunochromatographic antibody assay based on different M. bovis antigens, including MP… Leer más
Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
Research In Veterinary Science 110(2017)34-39.
Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-α and to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of f… Leer más