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Últimas publicaciones científicas SCI

15 de enero de 2021

Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System

Sensors 21:584.

     More effective methods to detect bovine tuberculosis, caused by Mycobacterium bovis, in wildlife, is of paramount importance for preventing disease spread to other wild animals, livestock, and human beings. In this study, we analyzed the volatile organic compounds emitted by fecal samples collected from free-ranging wild boar captured in Doñana National Park, Spain, with an electronic nose system based on organically-functionalized gold nanoparticles. The animals were separated by the age group for performing the analysis. Adult (>24 months) and sub-adult (12–24 months) animals were anesthetized before sample collection, whereas the juvenile (<12 months) animals were manually restrained while collecting the sample. Good accuracy was obtain… Leer más

Beleno-Saenz KJ., Caceres-Tarazona JM., Nol P., Jaimes-Mogollon AL., Gualdron-Guerrero OE., Duran-Acevedo CM., Barasona JA., Vicente J., Torres MJ., Welearegay TG., Osterlund L., Rhyan J. y Ionescu F.

16 de diciembre de 2020

Distinct African Swine Fever Virus Shedding in Wild Boar Infected with Virulent and Attenuated Isolates

Vaccine 8:767.

     Abstract: Since the reappearance of African swine fever virus (ASFV), the disease has spread in an unprecedented animal pandemic in Eurasia. ASF currently constitutes the greatest global problem for the swine industry. The wild boar (Sus scrofa) in which the pathogen has established wild self-sustaining cycles, is a key reservoir for ASFV, signifying that there is an urgent need to develop an effective vaccine against this virus. Current scientific debate addresses whether live attenuated vaccines (LAVs), which have shown promising results in cross-protection of susceptible hosts, may be feasible for vaccinations carried out owing to safety concerns. The objective of this study was, therefore, to compare the ASFV shedding in wild boar infec… Leer más

Kosowska A., Cadenas-Fernández E., Barroso-Arevalo S., Sanchez-Vizcaino JM. y Barasona JA..

15 de diciembre de 2020

Primary and promiscuous functions coexist during evolutionary innovation through whole protein domain acquisitions

ELife 9:e58061.

     Abstract Molecular examples of evolutionary innovation are scarce and generally involve point mutations. Innovation can occur through larger rearrangements, but here experimental data is extremely limited. Integron integrases innovated from double-strand- toward single-strand-DNA recombination through the acquisition of the I2 a-helix. To investigate how this transition was possible, we have evolved integrase IntI1 to what should correspond to an early innovation state by selecting for its ancestral activity. Using synonymous alleles to enlarge sequence space exploration, we have retrieved 13 mutations affecting both I2 and the multimerization domains of
IntI1. We circumvented epistasis constraints among them using a combinatorial l… Leer más

Escudero JA., Nivina A., Kemble HE., Loot C., Tenaillon O. y Mazel D.

10 de diciembre de 2020

Global emergence and evolutionary dynamics of bluetongue virus

Scientific Reports 10(1):21677.

     Bluetongue virus (BTV) epidemics are responsible for worldwide economic losses of up to US$ 3 billion. Understanding the global evolutionary epidemiology of BTV is critical in designing intervention programs. Here we employed phylodynamic models to quantify the evolutionary characteristics, spatiotemporal origins, and multi-host transmission dynamics of BTV across the globe. We inferred that goats are the ancestral hosts for BTV but are less likely to be important for cross-species transmission, sheep and cattle continue to be important for the transmission and maintenance of infection between other species. Our models pointed to China and India, countries with the highest population of goats, as the likely ancestral country for BTV emergen… Leer más

Alkhamis M., Aguilar-Vega C., Fountain-Jones NM., Lin K., Perez AM. y Sanchez-Vizcaino JM..

4 de diciembre de 2020

Histopathological and microbiological study of porcine lymphadenitis: contributions to diagnosis and control of the disease

Porcine Health Management 6(1):36.

     Tuberculosis like lesions (TBL) in free-range pigs are characterised by presenting a marked heterogeneity in pathology and microbiology features, with a notorious role of Mycobacterium tuberculosis complex (MTC), Trueperella pyogenes and different Streptococcus species. However, the capacity of these microorganism to spread to different organic cavities leading to a generalised disease is unknown. Therefore, this study evaluated the organic distribution of these agents in free-range pig carcasses whole condemned due to generalised TBL.A total of 37 totally condemned animals were analysed, and samples of lymph nodes and organs were obtained (n = 262) and subjected to histopathological and microbiological examination. In addition, T. pyogenes… Leer más

Cardoso-Toset F., Gomez-Laguna J., Gomez-Gascon L., Rodriguez-Gomez IM., Galan-Relaño A., Carrasco L., Tarradas C., Vela AI. y Luque I.

3 de diciembre de 2020

Molecular detection of extended spectrum B-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria

PLoS ONE 15(12):e0243130.

     The increase in antimicrobial resistance in developed and developing countries is a global public health challenge. In this context β-lactamase production is a major contributing factor to resistance globally. The aim of this study was to determine the prevalence of phenotypic and genotypic extended spectrum β-lactamases (ESBLs) in 296 E. coli isolates recovered from diarrhoeic children younger than five years in Kano whose susceptibility profile against 7 antimicrobials had been determined. The E. coli isolates were subjected to double disc synergy test for phenotypic ESBLs detection and ESBL associated genes (blaCTX-M, blaTEM and blaSHV) were detected using conventional PCR. Phenotypically, 12.8% (38/296) E. coli isolates presen… Leer más

Saka, K.H., Garcia-Soto S., Dabo NT., Lopez-Chavarrias V., Muhammad B., Ugarte-Ruiz M. y Alvarez J..

29 de noviembre de 2020

Computer Vision Applied to Detect Lethargy through Animal Motion Monitoring: A Trial on African Swine Fever in Wild Boar

Animals 10(12):E2241.

     Early detection of infectious diseases is the most cost-effective strategy in disease surveillance for reducing the risk of outbreaks. Latest deep learning and computer vision improvements are powerful tools that potentially open up a new field of research in epidemiology and disease control. These techniques were used here to develop an algorithm aimed to track and compute animal motion in real time. This algorithm was used in experimental trials in order to assess African swine fever (ASF) infection course in Eurasian wild boar. Overall, the outcomes showed negative correlation between motion reduction and fever caused by ASF infection. In addition, infected animals computed significant lower movements compared to uninfected animals. The … Leer más

Fernandez-Carrion E., Barasona JA., Sanchez A., Jurado C., Cadenas-Fernández E. y Sanchez-Vizcaino JM..

26 de noviembre de 2020

A one health framework to estimate the cost of antimicrobial resistance

Antimicrobial Resistance And Infection Control 9(1):187.

     Objectives/purpose: The costs attributable to antimicrobial resistance (AMR) remain theoretical and largely unspecified. Current figures fail to capture the full health and economic burden caused by AMR across human, animal, and environmental health; historically many studies have considered only direct costs associated with human infection from a hospital perspective, primarily from high-income countries. The Global Antimicrobial Resistance Platform for ONE-Burden Estimates (GAP-ON€) network has developed a framework to help guide AMR costing exercises in any part of the world as a first step towards more comprehensive analyses for comparing AMR interventions at the local level as well as more harmonized analyses for quantifying the full e… Leer más

Morel CM., Alm RA., Ardal C., Bandera A., Bruno GM., Carrara E., Colombo GL., de Kraker MEA., Essack S., Frost I., Gonzalez-Zorn B., Goossens H., Guardabassi L., Harbarth S., Jorgensen PS., Kanj SS., Kostyanev T., Laxminarayan R., Leonard F., Hara GL., Mendelson M., Mikulska M., Mutters NT., Outterson K., Rodriguez-Bano J., Tacconelli E., Scudeller L. y GAP-ONE network.

25 de noviembre de 2020

Direct PCR on tissue samples to detect Mycobacterium tuberculosis complex: an alternative to the bacteriological culture

Journal Of Clinical Microbiology 01404-20.

     Bovine tuberculosis (bTB) is an ongoing issue in several countries within the European Union (EU). Microbiological culture is the official confirmation technique for the presence of Mycobacterium tuberculosis complex (MTBC) members in bovine tissues, but several methodological issues, such as moderate sensitivity and long incubation times, require the development of more sensitive and rapid techniques. This study evaluates the analytical and diagnostic performance, comparatively to culture, of a real-time PCR targeting the MTBC-specific IS6110 transposon using a panel of bovine tissue samples sourced from the Spanish bTB eradication campaign. Robustness and repeatability were evaluated in an inter-laboratory trial between EU National Refere… Leer más

Lorente-Leal V., Liandris E., Pacciarini M., Botelho A., Kenny K., Loyo B., Fernandez R., Bezos J., Dominguez L., de Juan L. y Romero B..

13 de noviembre de 2020

Quantifying Antimicrobial Exposure in Dogs From a Longitudinal Study

Frontiers In Veterinary Science 7:545.

     Bacterial resistance to antimicrobials (AMR) is a growing public health threat, and exposure to antimicrobials (AMs) is, on the whole, a major risk factor for the occurrence of AMR. During the past decade, a limited number of studies about AM exposure in dogs have been published, showing a noticeable diversity regarding numerators (AMU), denominators (population at risk), and indicators. The aim of this study is to show that metrics based on the most easily recorded data about treatments and a follow-up design are a promising method for a preliminary assessment of AM exposure in companion animals when more detailed data are not available. To quantify AM exposure, two simple indicators were used: the number of treatments (Ts) per 100 dogs an… Leer más

Mendez M. y Moreno MA..

Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ZTA 218 SUAT 74 SUAT 19
2 ICM 202 ZTA 65 ZTA 15
3 MYC 193 MYC 55 MYC 10
4 SUAT 192 ICM 33 SAP 4
5 NED 70 NED 12 ICM 4
  VISAVET 923 295 73

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.606 ZTA 3.948 ZTA 3.45
2 SAP 2.947 SAP 3.075 NED 3.423
3 DICM 2.776 SUAT 2.867 ICM 3.036
4 SUAT 2.612 DICM 2.776 SUAT 2.994
5 MYC 2.553 ICM 2.731 SEVISEQ 2.749
VISAVET 2.790 3.138 3.910

ISI Scientific Publications
Total Last 60 mo.
1 Vet. Microbiol. 84 Transbound. Emerg. Dis. 51
2 Transbound. Emerg. Dis. 80 Front Vet Sci 39
3 Prev. Vet. Med. 71 PLoS ONE 27
4 PLoS ONE 54 Prev. Vet. Med. 20
5 Res. Vet. Sci. 46 Res. Vet. Sci. 18
  All journals 319   All journals 37