Publicaciones científicas 2002

DNA macrorestriction analysis by pulsed-field gel electrophoresis of Pseudomonas aeruginosa isolates from mastitis in dairy sheep

The Veterinary Record 151(22):670-2.

     A BROAD range of bacteria are responsible for mastitis in small ruminants, but Staphylococcus aureus and Staphylococcus epidermidis are the most prevalent species (Las Heras and others 1999a). Of the Gram-negative bacteria, species such as Mannheimia haemolytica and Pseudomonas aeruginosa are also responsible for a significant proportion of cases of mastitis in dairy sheep (Lafi and others 1998, Scott and Jones 1998, Rapoport and others 1999). P aeruginosa represents less than 10 per cent of the isolates from subclinical mastitis (Lafi and others 1998), but it is responsible for outbreaks of clinical mastitis with significant sanitary and economic repercussions (Las Heras and others 1999b, Rapoport and others 1999).… Leer más

Las Heras A., Vela AI., Fernandez E., Casamayor A., Dominguez L. y Fernandez-Garayzabal JF..

Metastatic Listeria monocytogenes infection of the peritoneum in mice with cyclosporine a-induced peritonitis

Journal Of Comparative Pathology 127(2-3):178-85.

     Inoculation of mice with Listeria monocytogenes intragastrically or by parenteral routes has not been reported to cause peritonitis. In this study, however, severe listerial peritonitis was induced in mice infected subcutaneously and treated intraperitoneally with cyclosporin A (Cs A) in an oil carrier. In both uninfected and listeria-infected mice, intraperitoneal administration of Cs A consistently produced overexpression of P-selectin in the peritoneal microvasculature and pyogranulomatous inflammation of the peritoneum, suggesting that Cs A causes endothelial damage. We suggest that in listeria-infected mice the non-specific irritant peritonitis induced by the intraperitoneal administration of Cs A results in transfer of listeria-infect… Leer más

Prats N., Lopez S., Domingo M., Briones V., Dominguez L. y Marco AJ.

Antimicrobial resistance among enterococci from pigs in three European countries

Applied And Environmental Microbiology 68(8):4127-9.

     Enterococci from pigs in Denmark, Spain, and Sweden were examined for susceptibility to antimicrobial agents and copper and the presence of selected resistance genes. The greatest levels of resistance were found among isolates from Spain and Denmark compared to those from Sweden, which corresponds to the amounts of antimicrobial agents used in food animal production in those countries. Similar genes were found to encode resistance in the different countries, but the tet(L) and tet(S) genes were more frequently found among isolates from Spain. A recently identified transferable copper resistance gene was found in all copper-resistant isolates from the different countries… Leer más

Aarestrup F., Hasman H., Jensen LB., Moreno MA., Herrero IA., Dominguez L., Finn M. y Franklin A.

Genotyping of Francisella tularensis strains by pulsed-field gel electrophoresis, amplified fragment length polymorphism fingerprinting, and 16S rRNA gene sequencing

Journal Of Clinical Microbiology 40(8):2964-72.

     We evaluated three molecular methods for identification of Francisella strains: pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) analysis, and 16S rRNA gene sequencing. The analysis was performed with 54 Francisella tularensis subsp. holarctica, 5 F. tularensis subsp. tularensis, 2 F. tularensis subsp. novicida, and 1 F. philomiragia strains. On the basis of the combination of results obtained by PFGE with the restriction enzymes XhoI and BamHI, PFGE revealed seven pulsotypes, which allowed us to discriminate the strains to the subspecies level and which even allowed us to discriminate among some isolates of F. tularensis subsp. holarctica. The AFLP analysis technique produced some degree of discriminat… Leer más

Garcia del Blanco N., Dobson M., Vela AI., de la Puente VA., Gutierrez CB., Hadfield TL., Kuhnert P., Frey J., Dominguez L. y Rodriguez-Ferri E.

Salmonella septicaemia in a beauty snake (Elaphe taeniura taeniura)

The Veterinary Record 151(1):28-9.

     The presence of Salmonella species in the intestines of reptiles has been documented since 1944 (Hinshaw and McNeil 1944). For many years it has been thought that diverse serotypes of salmonella represent part of the normal microbiota of the intestines of reptiles (Chiodini and Sundberg 1981, Dorrestein and others 2000). Nevertheless, it became obvious that besides being mere faecal carrier status or just minor subclinical infections in reptiles, salmonella can also result in severe, often fatal, diseases (Johnson-Delaney 1996), including gastroenteritis and septicaemia (Hinshaw and McNeil 1946, Boever and Williams 1975, Jacobson 1978, Frye 1991). This depends upon the virulence of the serotype, the nature and chronicity of the lesions, and… Leer más

Tellez S., Briones V., Gonzalez S., Garcia-Pena FJ., Altimira J., Vela AI., Blanco MM., Ballesteros C., Fernandez-Garayzabal JF., Dominguez L. y Goyache J..

Genomic deletions suggest a phylogeny for the Mycobacterium tuberculosis complex

The Journal Of Infectious Diseases 186(1):74-80.

     To better understand the evolution of the Mycobacterium tuberculosis complex, subspecies were tested for large sequence polymorphisms. Samples with greater numbers of deletions, without exception, were missing all the same regions that were deleted from samples with lesser numbers of deletions. Principal genetic groups based on single-nucleotide polymorphisms were restricted to one of the deletion-based groups, and isolates that shared genotypes based on molecular epidemiological markers were assigned almost exclusively to the same deletion type. The data provide compelling evidence that human tuberculosis did not originate from the present-day bovine form. Genomic deletions present themselves as an attractive modality to study the evolutio… Leer más

Mostowy S., Cousins DV., Brinkman J., Aranaz A. y Behr MA.

PCR detection and PFGE DNA macrorestriction analyses of clinical isolates of Pseudomonas anguilliseptica from winter disease outbreaks in sea bream Sparus aurata

Diseases Of Aquatic Organisms 50(1):19-27.

     A PCR-based detection system for Pseudomonas anguilliseptica was evaluated. The primer combination PAF-PAR (forward primer PAF = 5`-GACCTCGCCATTA-3`, reverse primer PAR = 5`-CTCAGCAGTTTTGAAAG-3`) gave a unique and specific amplification product of 439 bp at an annealing temperature of 46 degrees C with all the P. anguilliseptica isolates and strains (n = 56) but no amplification products were observed with any other Pseudomonas species or phylogenetically related bacteria tested. The PCR assay had a detection limit of 170 to 200 cells per PCR tube, which was improved 8-fold when the PCR amplification product was used as a nonradioacfive probe in blotting hybridization experiments. The PCR assay allowed the specific and reliable detection of… Leer más

Blanco MM., Gibello A., Vela AI., Moreno MA., Dominguez L. y Fernandez-Garayzabal JF..

Molecular characterization of pigmented and nonpigmented isolates of Mycobacterium avium subsp. paratuberculosis

Journal Of Clinical Microbiology 40(5):1798-804.

     Five pigmented isolates of Mycobacterium avium subsp. paratuberculosis were examined by pulsed-field gel electrophoresis (PFGE), IS900 restriction fragment length polymorphism (IS900-RFLP), and IS1311 polymorphism analysis using PCR. All of the pigmented isolates exhibited one of three distinct PFGE profiles with SnaBI, designated 9, 10, and 11, and with SpeI, designated 7, 8, and 9, which generated three multiplex profiles designated [9-7], [10-8], and [11-9]. All of the pigmented isolates had the same IS900-RFLP BstEII and PvuII profiles. The IS900-RFLP BstEII profile was new, but the IS900-RFLP PvuII profile corresponded to PvuII type 6 of a sheep strain described by Cousins and colleagues (D. V. Cousins, S. N. Williams, A. Hope, and G. … Leer más

Stevenson K., Hughes VM., de Juan L., Inglis NF., Wright F. y Sharp JM.

Streptococcus entericus sp. nov., isolated from cattle intestine

International Journal Of Systematic And Evolutionary Microbiology 52(Pt 2):665-9.

     Biochemical, molecular chemical and molecular genetic studies were performed on an unknown gram-positive, catalase-negative, coccus-shaped organism isolated from the intestine of a cow affected with catarrhal enteritis. The organism was tentatively identified as a streptococcal species based on results of cellular morphological and biochemical tests. 16S rRNA gene sequencing studies confirmed its provisional identification as a member of the genus Streptococcus, but the organism did not correspond to any recognized species of this genus. The nearest phylogenetic relatives of the unknown coccus from a calf were Streptococcus acidominimus and Streptococcus suis. The unknown bacterium, however, was distinguished from these species and other an… Leer más

Vela AI., Fernandez E., Lawson P., Latre MV., Falsen E., Dominguez L., Collins MD. y Fernandez-Garayzabal JF..

Unusual outbreak of clinical mastitis in dairy sheep caused by Streptococcus equi subsp. zooepidemicus

Journal Of Clinical Microbiology 40(3):1106-8.

     This work describes an outbreak of clinical mastitis affecting 13 of 58 lactating ewes due to Streptococcus equi subsp. zooepidemicus. S. equi subsp. zooepidemicus was isolated in pure culture from all milk samples. All the clinical isolates had identical biochemical profiles and antimicrobial susceptibility patterns and also exhibited indistinguishable macrorestriction patterns by pulsed-field gel electrophoresis, indicating that all cases of mastitis were produced by a single strain… Leer más

Las Heras A., Vela AI., Fernandez E., Legaz E., Dominguez L. y Fernandez-Garayzabal JF..

Mortality of rainbow trout [Oncorynchus mykiss (Walbaum)] associated with freshwater dinoflagellate bloom [Peridinium polonicum (Woloszynska)] in a fish farm

Aquaculture Research 33(2):141-145.

Roset J., Gibello A., Aguayo S., Dominguez L., Alvarez M., Fernandez-Garayzabal JF., Zapata A. y Munoz MJ.

Hpt, a bacterial homolog of the microsomal glucose- 6-phosphate translocase, mediates rapid intracellular proliferation in Listeria

Proceedings Of The National Academy Of Sciences Of The United States Of America 99(1):431-6.

     Efficient replication in vivo is essential for a microparasite to colonize its host and the understanding of the molecular mechanisms by which microbial pathogens grow within host tissues can lead to the discovery of novel therapies to treat infection. Here we present evidence that the foodborne bacterial pathogen Listeria monocytogenes, a facultative intracellular parasite, exploits hexose phosphates (HP) from the host cell as a source of carbon and energy to fuel fast intracellular growth. HP uptake is mediated by Hpt, a bacterial homolog of the mammalian translocase that transports glucose-6-phosphate from the cytosol into the endoplasmic reticulum in the final step of gluconeogenesis and glycogenolysis. Expression of the Hpt permease is… Leer más

Chico I., Suarez M., Gonzalez-Zorn B., Scortti M., Slaughuis J., Goebel W. y Vazquez-Boland JA.