Publicaciones científicas SCI 2017
Genetic diversity assessment of Tunisian Mycobacterium bovis population isolated from cattle
BMC Veterinary Research 13(1):393.
Background
The genetic diversity of M. bovis in Tunisia is still underestimated despite the implementation of an eradication program. The lack of data about spatial distribution of the M. bovis population hinders the control of bovine tuberculosis (bTB) progress. This study represents the largest molecular analysis of M. bovis isolates in Tunisia. It is aimed to upgrade the understanding of bTB epidemiology and the geographical distribution of the infection. Tuberculosis research was performed in cattle (n = 149) with TB-compatible lesions collected over 5 months from a slaughterhouse located in Sfax, Tunisia.
Results
Ninety-four animals were found to be infected by M. bovis and two others by M. caprae. Spol… Leer más
Djemal Saifeddine., Siala M., Smaoui S., Kammoun S., Marouane C., Bezos J., Messadi-Akrout F., Romero B. y Gdoura R.
Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain
Emerging Infectious Diseases 23(12):2013-2016.
Streptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associated with different clinical backgrounds. We also investigated erythromycin resistance mechanisms and phenotypes and virulence genes. We observed 2 emm types: emm12 (11 isolates) and emm77 (4 isolates). Similarly, we observed 2 genetic linages, sequence type (ST) 26 and ST63. Most isolates exhibited the M macrolide resistance phenotype and the mefA/ermB genotype. Isolates were grouped into 2 clones on the basis of emm-MLST-PFGE-virulence gene pro… Leer más
Vela AI., Villalon P., Saez-Nieto JA., Chacon G., Dominguez L. y Fernandez-Garayzabal JF..
Multi-Laboratory Evaluation of a Novel Lateral Flow Immunochromatographic Assay for Confirming Isolation of Mycobacterium bovis from Veterinary Diagnostic Specimens
Journal Of Clinical Microbiology 55(12):3411-3425.
A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for Mycobacterium bovis and M. caprae cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotyping or qPCR) in each laboratory; liquid (MGIT or BacT/Alert) and/or solid (Stonebrink, Coletsos or Lowenstein-Jensen) cultures were tested. In comparison to spoligotyping of acid-fast positive MGIT cultures, percentage agreement between positive LFD and spoligotyping results was excellent in two UK laboratories (97.7-100%), but lower in the Spanish context (76%) where spoligotyping was applied to MGIT cultures previously confirm… Leer más
Stewart LD., McCallan L., McNair J., McGoldrick A., Morris R., Moyen JL., de Juan L., Romero B., Alonso E., Parsons SDC., van Helden P., Araujo FR. y Grant IR.
Differentiation of Flavobacterium psychrophilum from Flavobacterium psychrophilum-like species by MALDI-TOF mass spectrometry
Research In Veterinary Science 115:345-352.
Rainbow trout fry syndrome (RTFS) is an important infectious disease caused by Flavobacterium psychrophilum affecting farmed salmonids worldwide. Other Flavobacterium psychrophilum-like species (F. plurextorum, F. oncorhynchi, F. tructae, F. collinsii and F. piscis) have been isolated from diseased rainbow trout fry suspected of RTFS although the epidemiological and clinical relevance of these pathogens are unknown. The objective of this study was to evaluate the potential use of MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time of Flight) Mass Spectrometry as method for specific identification of F. psychrophilum and its differentiation from other F. psychrophilum-like species isolated from diseased fish. Fifty-three isolates wer… Leer más
Perez-Sancho M., Vela AI., Wiklund T., Kostrzewa M., Dominguez L. y Fernandez-Garayzabal JF..
Equine viral arteritis in breeding and sport horses in central Spain
Research In Veterinary Science 115:88-91.
Equine viral arteritis (EVA) may have a high economic impact on breeding stud farms due to the occurrence of EVA-associated abortion outbreaks and the ability of the virus to persist in carrier stallions. While the consequences of EVA in premises with sport horses are usually less severe, the first confirmed outbreak of EVA in Spain occurred in a riding club in Barcelona, but no data on the seroprevalence of EVA in sport horses have been reported in Spain. Given the importance of both Spanish Purebred (SP) breeding horses and sport horses for Spain`s equine industry, the aim of this study was to determine and compare the seroprevalence of EVA in these two horse populations in central Spain. Serum samples from 155 SP breeding horses residing… Leer más
Cruz F., Newton R., Sanchez A., Ireland J., Mughini-Gras L., Moreno MA. y Fores P.
Development and evaluation of an interferon gamma assay for the diagnosis of tuberculosis in red deer experimentally infected with Mycobacterium bovis
BMC Veterinary Research 13(1):341.
BACKGROUND:
Red deer (Cervus elaphus) is regarded as an epidemiologically relevant host for Mycobacterium bovis (M. bovis) and closely related members of the Mycobacterium tuberculosis complex that cause animal tuberculosis (TB). The standard antemortem screening test for the detection of TB in deer is the intradermal tuberculin skin test, but the detection of interferon-gamma (IFNγ) produced by white blood cells exposed to M. bovis antigens can be used as an alternative or supplemental assay in most TB eradication/control programs. This study aims to develop an in-house sandwich ELISA for deer IFNγ, based on the cross-reactivity of the antibodies to both cervid and bovine IFNγ, and to evaluate the potential of this as… Leer más
Risalde MA., Thomas J., Sevilla I., Serrano M., Ortiz JA., Garrido J., Dominguez M., Dominguez L., Gortazar C. y Ruiz-Fons JF.
Genome Comparison of Erythromycin Resistant Campylobacter from Turkeys Identifies Hosts and Pathways for Horizontal Spread of erm(B) genes
Frontiers In Microbiology 8:2240.
Pathogens in the genus Campylobacter are the most common cause of food-borne bacterial gastro-enteritis. Campylobacteriosis, caused principally by Campylobacter jejuni and C. coli, is transmitted to humans by food of animal origin, especially poultry. As for many pathogens, antimicrobial resistance in Campylobacter is increasing at an alarming rate. Erythromycin prescription is the treatment of choice for clinical cases requiring antimicrobial therapy but this is compromised by mobility of the erythromycin resistance gene erm(B) between strains. Here we evaluate resistance to six antimicrobials in 170 Campylobacter isolates (133 C. coli and 37 C. jejuni) from turkeys. Erythromycin resistant isolates (n=85; 81 C. coli and 4 C. jejuni) were s… Leer más
Florez-Cuadrado D., Ugarte-Ruiz M., Meric G., Quesada A., Porrero MC., Pascoe B., Saez-Llorente JL., Lopez-Orozco G., Dominguez L. y Sheppard S.
Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis
Clinical Proteomics 14:36.
Bovine purified protein derivative (bPPD) and avian purified protein derivative (aPPD) are widely used for bovine tuberculosis diagnosis. However, little is known about their qualitative and quantitative characteristics, which makes their standardisation difficult. In addition, bPPD can give false-positive tuberculosis results because of sequence homology between Mycobacterium bovis (M. bovis) and M. avium proteins. Thus, the objective of this study was to carry out a proteomic characterisation of bPPD, aPPD and an immunopurified subcomplex from bPPD called P22 in order to identify proteins contributing to cross-reactivity among these three products in tuberculosis diagnosis… Leer más
Infantes-Lorenzo JA., Moreno I., Risalde MA., Roy A., Villar M., Romero B., Ibarrola N., de la Fuente J., Puentes E., de Juan L., Gortazar C., Bezos J., Dominguez L. y Dominguez M.
Streptococcus ovuberis sp. nov., isolated from a subcutaneous abscess in the udder of a sheep
International Journal Of Systematic And Evolutionary Microbiology 67(11):4340-4344.
One unidentified, Gram-stain-positive, catalase-negative coccus-shaped organism was recovered from a subcutaneous abscess of the udder of a sheep and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolate was tentatively assigned to the genus Streptococcus, although the organism did not appear to match any recognized species. 16S rRNA gene sequence comparison studies confirmed its identification as a member of the genus Streptococcus and showed that the nearest phylogenetic relatives of the unknown coccus corresponded to Streptococcus moroccensis and Streptococcus cameli (95.9 % 16S rRNA gene sequence similarity). The sodA sequence analysis showed less than 89.3 % sequenc… Leer más
Zamora L., Perez-Sancho M., Fernandez-Garayzabal JF., Orden JA., Dominguez-Bernal G., de la Fuente R., Dominguez L. y Vela AI..
Approaches and Perspectives for Development of African Swine Fever Virus Vaccines
Vaccines 5(4):35.
African swine fever (ASF) is a complex disease of swine, caused by a large DNA virus belonging to the family Asfarviridae. The disease shows variable clinical signs, with high case fatality rates, up to 100%, in the acute forms. ASF is currently present in Africa and Europe where it circulates in different scenarios causing a high socio-economic impact. In most affected regions, control has not been effective in part due to lack of a vaccine. The availability of an effective and safe ASFV vaccines would support and enforce control-eradication strategies. Therefore, work leading to the rational development of protective ASF vaccines is a high priority. Several factors have hindered vaccine development, including the complexity of the ASF vir… Leer más
Arias M., de la Torre A., Dixon L., Gallardo C., Jori F., Laddomada A., Martins C., Parkhouse RM., Revilla Y., Rodriguez F. y Sanchez-Vizcaino JM..
Body temperature and motion: Evaluation of an online monitoring system in pigs challenged with Porcine Reproductive & Respiratory Syndrome Virus
Research In Veterinary Science 114:482-488.
Highly contagious and emerging diseases cause significant losses in the pig producing industry worldwide. Rapid and exact acquisition of real-time data, like body temperature and animal movement from the production facilities would enable early disease detection and facilitate adequate response. In this study, carried out within the European Union research project RAPIDIA FIELD, we tested an online monitoring system on pigs experimentally infected with the East European subtype 3 Porcine Reproductive & Respiratory Syndrome Virus (PRRSV) strain Lena. We linked data from different body temperature measurement methods and the real-time movement of the pigs. The results showed a negative correlation between body temperature and movement of the … Leer más
Suli T., Halas M., Benyeda Z., Boda R., Belak S., Martinez-Aviles M., Fernandez-Carrion E. y Sanchez-Vizcaino JM..
MCR-2-mediated plasmid-borne polymyxin resistance most likely originates from Moraxella pluranimalium
The Journal Of Antimicrobial Chemotherapy 72(10):2947-2949.
The recent identification of the first plasmid-mediated polymyxin resistance determinants, namely the MCR-1 and MCR-2 enzymes, has constituted an ultimate threat of pandrug resistance in Gram-negative organisms.1–3 These enzymes have been reported only in Enterobacteriaceae, mainly in Escherichia coli and Klebsiella pneumoniae; however, there have been reports from other enterobacterial genera, including Enterobacter, Salmonella and Shigella.1 The mcr-1 and mcr-2 genes have both been identified from cattle and pigs.1–3 In addition, the mcr-1 gene has been identified from chickens,4 but also from river samples and vegetables… Leer más
Poirel L., Kieffer N., Fernandez-Garayzabal JF., Vela AI., Larpin Y. y Nordmann P.
Vaccination as a control strategy against Salmonella infection in pigs: A systematic review and meta-analysis of the literature
Research In Veterinary Science 114:86-94.
Consumption or handling of improperly processed or cooked pork is considered one of the top sources for foodborne salmonellosis, a common cause of intestinal disease worldwide. Asymptomatic carrier pigs may contaminate pork at slaughtering; therefore, pre-harvest reduction of Salmonella load can contribute to reduce public health risk. Multiple studies have evaluated the impact of vaccination on controlling Salmonella in swine farms, but results are highly variable due to the heterogeneity in vaccines and vaccination protocols. Here, we report the results of an inclusive systematic review and a meta-analysis of the peer-reviewed scientific literature to provide updated knowledge on the potential effectiveness of Salmonella vaccination. A to… Leer más
de la Cruz ML., Conrado I., Nault A., Perez AM., Dominguez L. y Alvarez J..
How does temperature influences the development of lactococcosis? Transcriptomic and immunoproteomic in vitro approaches
Journal Of Fish Diseases 40(10):1285-1297.
Lactococcus garvieae is the aetiological agent of lactococcosis, a haemorrhagic septicaemia that affects marine and freshwater fish, with special incidence and economic relevance in farmed rainbow trout. Water temperature is one of the most important predisposing factors in the development of lactococcosis outbreaks. Lactococcosis in trout usually occur when water temperatures rise to about 18 °C, while fish carriers remain asymptomatic at temperatures below 13 °C. The aim of this work was to analyse the differences in the complete transcriptome response of L. garvieae grown at 18 °C and at 13 °C and to identify the immunogenic proteins expressed by this bacterium at 18 °C. Our results show that water temperature influences the expression o… Leer más
Castro R., Reguera-Brito M., Lopez-Campos GH., Blanco MM., Aguado-Urda M., Fernandez-Garayzabal JF. y Gibello A.
Update on the Risk of Introduction of African Swine Fever by Wild Boar into Disease-Free European Union Countries
Transboundary And Emerging Diseases 64(5):1424-1432.
Despite efforts to prevent the appearance and spread of African swine fever (ASF) in the European Union, several Member States are now affected (Lithuania, Poland, Latvia and Estonia). Disease appearance in 2014 was associated with multiple entrances linked to wild boar movement from endemic areas (EFSA Journal, 8, 2015, 1556), but the risk of new introductions remains high (Gallardo et al., Porcine Health Management, 1, and 21) as ASF continues to be active in endemic countries (Russian Federation, Belarus and Ukraine). Since 2014, the number of ASF notifications has increased substantially, particularly in wild boar (WB), in parallel with slow but constant geographical advance of the disease. This situation suggests a real risk of further… Leer más
Bosch J., Rodriguez A., Iglesias I., Munoz MJ., Jurado C., Sanchez-Vizcaino JM. y de la Torre A.
Parenteral Vaccination with Heat-Inactivated Mycobacterium bovis Reduces the Prevalence of Tuberculosis-Compatible Lesions in Farmed Wild Boar
Transboundary And Emerging Diseases 64(5):e18-e21.
In 2012, a wild boar (Sus scrofa) tuberculosis (TB) control programme was set up in a wild boar farm by means of intramuscular (IM) vaccination with a heat-inactivated Mycobacterium bovis vaccine (IV). The goal was to assess safety and efficacy of the parenterally administered IV in a large farm setting with natural M. bovis circulation. Based on preceding results under laboratory conditions, we hypothesized that vaccinated piglets would show smaller scores of TB-compatible lesions (TBCL) than unvaccinated controls. After vaccination, no adverse reactions were detected by visual inspection or at post-mortem examination (n = 668 and 97, respectively). Post-mortem data on TBCL were available for 97 vaccinated wild boar and 182 controls. The o… Leer más
Diez-Delgado I., Rodriguez O., Boadella M., Garrido JM., Sevilla I., Bezos J., Juste R., Dominguez L. y Gortazar C.
Identification of a New Genotype of African Swine Fever Virus in Domestic Pigs from Ethiopia
Transboundary And Emerging Diseases 64(5):1393-1404.
African swine fever (ASF) is an important emerging transboundary animal disease (TAD), which currently has an impact on many countries in Africa, Eastern Europe, the Caucasus and the Russian Federation. The current situation in Europe shows the ability of the virus to rapidly spread, which stands to threaten the global swine industry. At present, there is no viable vaccine to minimize spread of the disease and stamping out is the main source of control. In February 2011, Ethiopia had reported its first suspected outbreaks of ASF. Genomic analyses of the collected ASF virus (ASFV) strains were undertaken using 23 tissue samples collected from domestic swine in Ethiopia from 2011 to 2014. The analysis of Ethiopian ASFVs partial p72 gene seque… Leer más
Achenbach JE., Gallardo C., Nieto-Pelegrin E., Rivera B., Degefa T., Arias M., Jenberie S., Mulisa DD., Gizaw D., Gelaye E., Chibssa TR., Belaye A., Loitsch A., Forsa M., Yami M., Diallo A., Soler A., Lamien CE. y Sanchez-Vizcaino JM..
Serosurvey Reveals Exposure to West Nile Virus in Asymptomatic Horse Populations in Central Spain Prior to Recent Disease Foci
Transboundary And Emerging Diseases 64(5):1387-1392.
West Nile fever/encephalitis (WNF) is an infectious disease affecting horses, birds and humans, with a cycle involving birds as natural reservoirs and mosquitoes as transmission vectors. It is a notifiable disease, re-emerging in Europe. In Spain, it first appeared in horses in the south (Andalusia) in 2010, where outbreaks occur every year since. However, in 2014, an outbreak was declared in horses in central Spain, approximately 200 km away from the closest foci in Andalusia. Before that, evidence of West Nile virus (WNV) circulation in central Spain had been obtained only from wildlife, but never in horses. The purpose of this work was to perform a serosurvey to retrospectively detect West Nile virus infections in asymptomatic horses in … Leer más
Abad-Cobo A., Llorente F., Barbero MD., Cruz F., Fores P. y Jimenez-Clavero MA.
Motion-based video monitoring for early detection of livestock diseases: The case of African swine fever
PLoS ONE 12(9):e0183793.
Early detection of infectious diseases can substantially reduce the health and economic impacts on livestock production. Here we describe a system for monitoring animal activity based on video and data processing techniques, in order to detect slowdown and weakening due to infection with African swine fever (ASF), one of the most significant threats to the pig industry. The system classifies and quantifies motion-based animal behaviour and daily activity in video sequences, allowing automated and non-intrusive surveillance in real-time. The aim of this system is to evaluate significant changes in animals` motion after being experimentally infected with ASF virus. Indeed, pig mobility declined progressively and fell significantly below pre-i… Leer más
Fernandez-Carrion E., Martinez-Aviles M., Ivorra B., Martinez-Lopez B., Ramos AM. y Sanchez-Vizcaino JM..
Genomic Plasticity of Vibrio cholerae
International Microbiology 20(3):138-148.
Vibrio cholerae is one of the deadliest pathogens in the history of humankind. It is the causative agent of cholera, a disease characterized by a profuse and watery diarrhoea that still today causes 95.000 deaths worldwide every year. V. cholerae is a free living marine organism that interacts with and infects a variety of organisms, from amoeba to humans, including insects and crustaceans. The complexity of the lifestyle and ecology of V. cholerae suggests a high genetic and phenotypic plasticity. In this review, we will focus on two peculiar genomic features that enhance genetic plasticity in this bacterium: the division of its genome in two different chromosomes and the presence of the superintegron, a gene capture device that acts as a … Leer más
Escudero JA. y Mazel D.
The response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain
Veterinary Microbiology 208:195-202.
Deer species (family Cervidae) are often part of the Mycobacterium tuberculosis complex maintenance host community, and tuberculosis (TB) control in deer, including vaccination, is consequently an area of ongoing research. However, most research into deer vaccination against TB is focused on using the live bacillus Calmette Guerin (BCG). Oral inactivated vaccines represent an interesting alternative to either oral or parenteral BCG, since neither diagnostic cross-reactions nor vaccine strain survival are likely to occur. In order to describe the red deer response to heat-inactivated M. bovis (IV) as compared to BCG and to unvaccinated controls (n = 5/group), we ran an experiment with five month-old vaccinated red deer, which were challenged… Leer más
Thomas J., Risalde MA., Serrano M., Sevilla I., Geijo M., Ortiz JA., Fuertes M., Ruiz-Fons JF., de la Fuente J., Dominguez L., Juste R., Garrido J. y Gortazar C.
Short communication: Isolation frequency of bacteria causing lymphadenitis and abscesses in small ruminants in central Spain
Small Ruminant Research 154:5-8.
Infectious lymphadenitis in small ruminants caused by Corynebacterium pseudotuberculosis and Staphylococcus aureus subsp. anaerobius are widely distributed throughout the world, and result in significant economic losses. Trueperella pyogenes has also been associated with lymphadenitis in sheep and goats. In order to determinate the isolation frequency of the different agents associated with lymphadenitis and abscesses, 171 pus samples (135 from sheep and 36 from goats) from 46 flocks were investigated. Isolated bacteria were identified by MALDI-TOF method. S. aureus subsp. anaerobius was the most frequently detected agent. It was identified in 76 animals (59 sheep and 17 goats) from 24 of the surveyed flocks. Of these infected animal, 25 (3… Leer más
de la Fuente R., de las Heras M., Torrijos C., Diez de Tejada P., Perez-Sancho M., Carrion FJ., Orden JA. y Dominguez-Bernal G.
The mobile dso-gene-sso element in rolling circles plasmids of staphylococci reflects the evolutionary history of its resistance gene
Letters In Applied Microbiology 65(3):192-198.
The qacC and lnuA genes of Staphylococcus species were recently proposed to comprise a mobile element when residing on rolling-circle plasmids. Here we present other examples of resistance genes on staphylococcal rolling-circle plasmids, including fosB producing resistance to fosfomycin, cat resulting in resistance to chloramphenicol, and cadB for resistance to the toxic heavy metal cadmium. For three of these genes (qacC, lnuA and fosB) evidence was obtained that the genes have spread between different plasmid backgrounds. The lack of mutations in qacC suggests that the spread occurred relatively recently, while the build up of mutations in lnuA and fosB suggests their mobilization occurred in the more distant past. These observations can … Leer más
Wassenaar TM. y Cabal A.
Phylogenomic analysis of the complete sequence of a gastroenteritis-associated cetacean adenovirus (bottlenose dolphin adenovirus 1) reveals a high degree of genetic divergence
Infection Genetics And Evolution 53:47-55.
Adenoviruses are common pathogens in vertebrates, infecting a wide range of hosts, but only having rarely been detected and correlated with disease in cetaceans. This article describes the first complete genomic sequence of a cetacean adenovirus, bottlenose dolphin adenovirus 1 (BdAdV-1), detected in captive bottlenose dolphin population (Tursiops truncatus) suffering from self-limiting gastroenteritis. The complete genome sequence of BdAdV-1 was recovered from data generated by high-throughput sequencing and validated by Sanger sequencing. The genome is 34,080bp long and has 220 nucleotides long inverted terminal repeats. A total of 29 coding sequences were identified, 26 of which were functionally annotated. Among the unusual features of … Leer más
Malmberg M., Rubio-Guerri C., Hayer J., Garcia-Parraga D., Nieto-Pelegrin E., Melero M., Alvaro T., Valls M., Sanchez-Vizcaino JM., Belak S. y Granberg F.
Application of a specific quantitative real-time PCR (qPCR) to identify Leishmania infantum DNA in spleen, skin and hair samples of wild Leporidae
Veterinary Parasitology 243:92-99.
The aim of this study was to compare a quantitative real-time PCR (qPCR) validated for the detection of Leishmania infantum in dogs with a nested PCR but in wild Leporidae. Additionally, L. infantum results from indirect immunofluorescent antibody test (IFAT) and in vitro culture were also compared with qPCR. Different samples (spleen, skin and hair) recovered from 224 European rabbits and 70 Iberian hares from two green areas of Madrid Council were analysed for the detection of L. infantum. The presence of Leishmania kDNA was detected by qPCR in 58 out of 221 (26.24%), 162 out of 203 (79.8%) and 22 out of 33 (66.67%) analysed rabbits on spleen, skin and hair samples, respectively; and in 7 out of 69 (10.14%), 39 out of 70 (55.71%) and 17 o… Leer más
Ortega-García MV., Moreno I., Dominguez M., de la Cruz ML., Martin AB., Rodriguez-Bertos A., Lopez R., Navarro A., Gonzalez S., Mazariegos M., Goyache J., Dominguez L. y Garcia N..
Oral Vaccination with Heat-Inactivated Mycobacterium bovis Does Not Interfere with the Antemortem Diagnostic Techniques for Tuberculosis in Goats
Frontiers In Veterinary Science 4(124):1-7.
Vaccination against tuberculosis (TB) is prohibited in cattle or other species subjected to specific TB eradication campaigns, due to the interference that it may cause with the official diagnostic tests. However, immunization with a heat-inactivated (HI) Mycobacterium bovis vaccine via the oral route has been suggested to overcome this issue. In this study, the main goal was to assess the interference of the HI vaccine by different routes of administration using a previous vaccination and re-vaccination (boosting) protocol. TB-free kid goats were divided into three groups: oral (n = 16), intramuscular (IM; n = 16), and control (n = 16). Results showed that there was a significant difference in the percentage of animals positive to the sing… Leer más
Roy A., Risalde MA., Casal C., Romero B., de Juan L., Menshawy AMS., Diez-Guerrier A., Juste R., Garrido JM., Sevilla I., Gortazar C., Dominguez L. y Bezos J..
Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015
Eurosurveillance 22(31):30586.
Colistin resistance genes mcr-3 and mcr-1 have been detected in an Escherichia coli isolate from cattle faeces in a Spanish slaughterhouse in 2015. The sequences of both genes hybridised to same plasmid band of ca 250 kb, although colistin resistance was non-mobilisable. The isolate was producing extended-spectrum beta-lactamases and belonged to serotype O9:H10 and sequence type ST533. Here we report an mcr-3 gene detected in Europe following earlier reports from Asia and the United States.… Leer más
Hernandez M., Iglesias MR., Rodriguez-Lazaro D., Gallardo A., Quijada NM., Miguela-Villoldo P., Campos MJ., Piriz S., Lopez G., De Frutos C., Saez-Llorente JL., Ugarte-Ruiz M., Dominguez L. y Quesada A.
Technological advances in veterinary diagnostics: opportunities to deploy rapid decentralised tests to detect pathogens affecting livestock
Revue Scientifique Et Technique 36(2):479-498.
Sustainable food production capable of feeding a growing human population is a significant global challenge, and is a priority encompassed within the United Nations Millennium Development Goal to `eradicate extreme poverty and hunger`. Infectious diseases reduce the productivity of farm animals, and the globalised trade of animals and their products increases the threat of disease incursion. Accurate and rapid diagnostic tests are an essential component of contingency plans to detect, control and eradicate such diseases. Diagnosis involves a `pipeline` that normally starts with clinical suspicion, followed by collecting samples, transporting specimens to a centralised laboratory setting (e.g. national/international Reference Laboratories), … Leer más
Howson ELA., Soldan A., Webster K., Beer M., Zientara S., Belak S., Sanchez-Vizcaino JM., Van Borm S., King DP. y Fowler VL.
Isolation of Brachyspira species from farmed wild boar in Spain
The Veterinary Record 181(6):115.
Bacteria belonging to the genus Brachyspira are gram-negative, anaerobic, spirochaetes adapted to occupy specialised niches in the large intestines of animals, including swine.1 Brachyspira species cause important digestive diseases such as swine dysentery (Brachyspira hyodysenteriae) and porcine intestinal spirochetosis (Brachyspira pilosicoli), which have a significant economic impact on the swine industry. Spain has major problems associated with swine dysentery, as demonstrated by Carvajal and others, who showed that more than 30% of Spanish farms and 12% of porcine faecal specimens tested positive for B hyodysenteriae. 2 Domestic pigs and wild boars belong to the same species, and are susceptible to the same pathogens.3 In fact, increa… Leer más
Vadillo S., San-Juan C., Calderon M., Risco D., Fernandez-Llario P., Perez-Sancho M., Redondo E., Hurtado MA. y Igeno I.
Escherichia coli ST167 carrying plasmid mobilisable mcr-1 and blaCTX-M-15 resistance determinants isolated from a human respiratory infection
International Journal Of Antimicrobial Agents 50(2):285-286.
The emergence of polymyxin resistance in Enterobacteriaceae is a matter of major concern and has instigated the recommendation for its surveillance since it compromises the use of colistin, a last-resort antibiotic.… Leer más
Sanchez-Benito R., Iglesias MR., Quijada NM., Campos MJ., Ugarte-Ruiz M., Hernandez M., Pazos C., Rodriguez-Palenzuela P., Garduno E., Dominguez L. y Quesada A.
Effect of seafood peptones on biomass and metabolic activity by Enterococcus faecalis DM19
LWT - Food Science And Technology 81:94-100.
Eight seafood protein hydrolysates (SPHs) obtained from squid, shrimp and fish gelatin were incorporated as substitutes of peptones in culture media in order to evaluate its effect on survival and metabolic activity (lactic acid, acetic acid and bacteriocins production) of Enterococcus faecalis DM19. The substitution of commercial peptones in culture media by either a shrimp hydrolysate prepared with Protamex, or by squid protein hydrolysates prepared with Esperase or Alkaline protease, stimulated E. faecalis DM19 growth up to 16%. The incorporation of SPHs, mainly from shrimp, in the culture media significantly increased production of lactic and acetic acids in more than 60%. Furthermore, the media containing SPHs stimulated antimicrobial … Leer más
Djellouli M., Martinez-Alvarez O., Arancibia MY., Florez-Cuadrado D., Ugarte-Ruiz M., Dominguez L., Zadi-Karam H., Karam N. y Roudj S.
Serological Surveillance and Direct Field Searching Reaffirm the Absence of Ornithodoros Erraticus Ticks Role in African Swine Fever Cycle in Sardinia
Transboundary And Emerging Diseases 64(4):1322-1328.
African swine fever (ASF), one of the most important diseases of swine, has been endemic in the Italian island of Sardinia for more than 35 years. During these decades, several strategies and eradication efforts have been implemented in the island with limited success. Strong climatic and ecological similarities exist between Sardinia and one area of the Iberian Peninsula where Ornithodoros erraticus ticks were involved in the persistence of ASF from 1960 to 1995. This fact leads to the hypothesis that, potentially, Ornithodoros ticks could be also involved in the ASF cycle in Sardinia, thus accounting for some of the reoccurring ASF outbreaks in this island. Initial efforts aimed at detection of Ornithodoros ticks in Sardinia were performe… Leer más
Mur L., Iscaro C., Cocco M., Jurado C., Rolesu S., de Mia GM., Feliziani F., Perez-Sanchez R., Oleaga A. y Sanchez-Vizcaino JM..
Insights into a novel blaKPC-2 -encoding IncP-6 plasmid reveal carbapenem-resistance circulation in several Enterobacteriaceae species from wastewater and a hospital source in Spain
Frontiers In Microbiology 8:1143.
Untreated wastewater, particularly from hospitals and other healthcare facilities, is considered to be a reservoir for multidrug-resistant bacteria. However, its role in the spread of antibiotic resistances in the human population remains poorly investigated. We used whole genome sequencing (WGS) to analyze 25 KPC-2-producing Enterobacteriaceae isolates from sewage water collected during a 3-year period and three clinical Citrobacter freundii isolates from a tertiary hospital in the same collection area in Spain. We detected a common, recently described, IncP-6 plasmid carrying the gene blaKPC-2 in 21 isolates from both sources. The plasmid was present in diverse environmental bacterial species of opportunistic pathogens such as C. freundii… Leer más
Yao Y., Lazaro-Perona F., Falgenhauer L., Valverde A., Imizalioglu C., Dominguez L., Canton R., Mingorance J. y Chakraborty T.
Antibody detection tests improve the sensitivity of tuberculosis diagnosis in cattle
Research In Veterinary Science 112(2017):214-221.
We evaluated the sensitivity (Se) of the single cervical intradermal tuberculin (SIT) test, two interferon-gamma (IFN-γ) assays and three different antibody detection techniques for bovine tuberculosis (bTB) diagnosis in 131 mixed beef breed cattle. The results of the diagnostic techniques performed over the whole herd, and over the animals confirmed as infected based on the presence of lesions compatible with the disease and/or M. bovis isolation were compared to determine apparent prevalence (AP) and Se.
The Se of the SIT test (severe interpretation) was 63.7% (95% CI, 54.54–72.00), while the Se of the IFN-γ assays ranged between 60.2% and 92%. The proportion of infected cattle detected by the different antibody detection… Leer más
Casal C., Infantes-Lorenzo JA., Risalde MA., Diez-Guerrier A., Dominguez M., Moreno I., Romero B., de Juan L., Saez-Llorente JL., Juste R., Gortazar C., Dominguez L. y Bezos J..
First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis
Journal Of Equine Veterinary Science 53:30-37.
Strangles is one of the most frequently diagnosed equine respiratory infectious diseases in the world. It is caused by Streptococcus equi subsp. equi (S. equi), and it is an acute infection characterized by pyrexia, nasal discharge, pharyngitis, and abscessation of lymph nodes. Frequently, healthy horses might continue to harbor S. equi after clinical recovery. Although the genetic distance between S. equi isolates is short, strains can be differentiated by pulsed-field gel electrophoresis (PFGE) and single locus sequence typing for epidemiological studies. The aim of this study was to characterize by PFGE Argentine isolates of S. equi obtained from horses with acute strangles and those that had recovered. Bacterial isolation and identifica… Leer más
Bustos CP., Guida N., Casamayor A., Munoz AJ., Fernandez-Garayzabal JF. y Vela AI..
Human influence and biotic homogenization drive the distribution of Escherichia coli virulence genes in natural habitats
MicrobiologyOpen 6(3):1-10.
Cattle are the main reservoirs for Shiga-toxin-producing Escherichia coli (STEC), the only known zoonotic intestinal E. coli pathotype. However, there are other intestinal pathotypes that can cause disease in humans, whose presence has been seldom investigated. Thus, our aim was to identify the effects of anthropic pressure and of wild and domestic ungulate abundance on the distribution and diversity of the main human E. coli pathotypes and nine of their representative virulence genes (VGs). We used a quantitative real-time PCR (qPCR) for the direct detection and quantification of the genus-specific gene uidA, nine E. coli VGs (stx1, sxt2, eae, ehxA, aggR, est, elt, bfpA, invA), as well as four genes related to O157:H7 (rfbO157 , fliCH7 ) a… Leer más
Cabal A., Vicente J., Alvarez J., Barasona JA., Boadella M., Dominguez L. y Gortazar C.
Highly Tigecycline-Resistant Klebsiella pneumoniae Sequence Type 11 (ST11) and ST147 Isolates from Companion Animals
Antimicrobial Agents And Chemotherapy 61(6):e02640-16.
In this study we have characterized two tigecycline resistant Klebsiella pneumoniae clones isolated from dog urine samples. The isolates were genetically unrelated, belonging to sequence types ST11 and ST147, both classically related to human isolates. To the best of our knowledge, this is the first identification of tigecycline resistant isolates from animals. We unveil the worrisome circulation among animals of bacterial clones resistant to this last resort antibiotic… Leer más
Ovejero CM., Escudero JA., Thomas-Lopez D., Hoefer A., Moyano G., Montero N., Martin-Espada C. y Gonzalez-Zorn B..
Jeotgalibaca porci sp. nov. and Jeotgalibaca arthritidis sp. nov., two new species isolated from pigs and emended description of the genus Jeotgalibaca
International Journal Of Systematic And Evolutionary Microbiology 67(5):1473-1477.
Biochemical and molecular genetic studies were performed on two novel Gram-positive catalase-negative coccus-shaped organisms isolated from liquid joint samples of two pigs. The microorganisms were not identified as a recognized species based on their cellular, morphological and biochemical tests. 16S rRNA gene sequence comparison studies allowed their identifications as members of the genus Jeotgalibaca, but the organisms were different to Jeotgalibaca dankookensis, the single species of the genus. The two microorganisms shared 96.3% and 96.9% 16S rRNA gene sequence similarity values with their nearest phylogenetic relative, J. dankookensis. The novel bacterial isolates were distinguished from J. dankookensis using biochemical tests. Based… Leer más
Vela AI., Perez-Sancho M., Zamora L., Dominguez L. y Fernandez-Garayzabal JF..
Tuberculin Skin Testing Boosts Interferon Gamma Responses to DIVA Reagents in Mycobacterium bovis-Infected Cattle
Clinical And Vaccine Immunology 24(5):e00551-16.
BCG vaccination sensitises cattle to bovine tuberculin which compromises the use of the current bovine TB surveillance tests. Although the performance of a blood test (that utilises antigens expressed by Mycobacterium bovis but not by BCG) capable of discriminating infected from vaccinated animals (DIVA interferon-gamma test, DIT) has been evaluated in naturally infected TB field reactors, there is a need to perform similar analysis in a BCG vaccinated/M. bovis infected population. Furthermore, we explored different scenarios under which a DIT may be implemented alongside BCG vaccination: (i) serial testing to resolve potential false positive skin test results; or (ii) standalone test to replace the single intradermal comparative cervical t… Leer más
Jones GJ., Coad M., Khatri B., Bezos J., Parlane NA., Buddle BM., Villareal-Ramos B., Hewinson RG. y Vordermeier M.
Characterization of Pasteurella multocida associated with ovine pneumonia using multi-locus sequence typing (MLST) and virulence-associated gene profile analysis and comparison with porcine isolates
Veterinary Microbiology 204:180-187.
Pasteurella multocida is a pathogen causing disease in a wide range of hosts including sheep and pigs. Isolates from ovine pneumonia were characterized by MLST (Multi-host and RIRDC databases) and virulence-associated gene (VAG) typing and compared with porcine isolates. Ovine and porcine isolates did not share any STs as determined by both schemes and exhibited different VAG profiles. With the Multi-host database, sixteen STs were identified among 43 sheep isolates with two STs (ST50 and ST19) comprising 53.5% of the isolates, and seven MLST genotypes (ST3, ST11 and ST62 included 75% of the isolates) among the 48 pig isolates. The most frequent VAG profile among sheep isolates was tbpA+/toxA+ (69.8% of isolates) and pfhA+ (62.5%) and hgbB+… Leer más
Garcia-Alvarez A., Vela AI., San Martin E., Chaves F., Fernandez-Garayzabal JF., Dominguez L. y Cid D.
Evaluation of the Mycobacterium tuberculosis SO2 vaccine using a natural tuberculosis infection model in goats
The Veterinary Journal 223:60-67.
The development of new vaccines against animal tuberculosis (TB) is a priority for improving the control and eradication of this disease, particularly in those species not subjected to compulsory eradication programmes. In this study, the protection conferred by the Mycobacterium tuberculosis SO2 experimental vaccine was evaluated using a natural infection model in goats. Twenty-six goats were distributed in three groups: (1) 10 goats served as a control group; (2) six goats were subcutaneously vaccinated with BCG; and (3) 10 goats were subcutaneously vaccinated with SO2. Four months after vaccination, all groups were merged with goats infected with Mycobacterium bovis or Mycobacterium caprae, and tested over a 40 week period using a tuberc… Leer más
Bezos J., Casal C., Alvarez J., Roy A., Romero B., Rodriguez-Bertos A., Barcena C., Diez-Guerrier A., Juste R., Gortazar C., Puentes E., Aguilo N., Martin C., de Juan L. y Dominguez L..
Risk mapping of West Nile virus circulation in Spain, 2015
Acta Tropica 169:163-169.
West Nile fever is an emergent disease in Europe. The objective of this study was to conduct a predictive risk mapping of West Nile Virus (WNV) circulation in Spain based on historical data of WNV circulation. Areas of Spain with evidence of WNV circulation were mapped based on data from notifications to the surveillance systems and a literature review. A logistic regression-based spatial model was used to assess the probability of WNV circulation. Data were analyzed at municipality level. Mean temperatures of the period from June to October, presence of wetlands and presence of Special Protection Areas for birds were considered as potential predictors. Two predictors of WNV circulation were identified: higher temperature [adjusted odds rat… Leer más
Sanchez A., Amela C., Fernandez-Carrion E., Martinez-Aviles M., Sanchez-Vizcaino JM. y Sierra-Moros MJ.
In-Depth Characterization and Functional Analysis of Clonal Variants in a Mycobacterium tuberculosis Strain Prone to Microevolution
Frontiers In Microbiology 8:694.
The role of clonal complexity has gradually been accepted in infection by Mycobacterium tuberculosis (MTB), although analyses of this issue are limited. We performed an in-depth study of a case of recurrent MTB infection by integrating genotyping, whole genome sequencing, analysis of gene expression and infectivity in in vitro and in vivo models. Four different clonal variants were identified from independent intrapatient evolutionary branches. One of the single-nucleotide polymorphisms in the variants mapped in mce3R, which encodes a repressor of an operon involved in virulence, and affected expression of the operon. Competitive in vivo and in vitro co-infection assays revealed higher infective efficiency for one of the clonal variants. A … Leer más
Navarro Y., Perez-Lago L., Herranz M., Sierra O., Comas I., Sicilia J., Bouza E. y Garcia de Viedma D.
Spread of mcr-1-carrying Enterobacteriaceae in sewage water from Spain
The Journal Of Antimicrobial Chemotherapy 72(4):1050-1053.
OBJECTIVES:
The mobile colistin resistance gene mcr-1 has been identified worldwide in human and animal sources, while its occurrence in the environment is still largely unknown. The aim of this study was to investigate the presence of mcr-1-harbouring Enterobacteriaceae in water samples obtained from rivers and waste water treatment plants in the area of Barcelona, Spain.
METHODS:
The presence of mcr-1 was detected by PCR. Bacterial identification was performed via MALDI-TOF MS. Resistance to colistin was determined by a broth dilution method. The epidemiological relationship between the positive isolates was assessed with PFGE and ST was determined by MLST. Plasmid characterization was performed by transformation expe… Leer más
Ovejero CM., Delgado-Blas JF., Calero-Caceres W., Muniesa M. y Gonzalez-Zorn B..
Early Detection of Infection in Pigs through an Online Monitoring System
Transboundary And Emerging Diseases 64(2):364-373.
Late detection of emergency diseases causes significant economic losses for pig producers and governments. As the first signs of animal infection are usually fever and reduced motion that lead to reduced consumption of water and feed, we developed a novel smart system to monitor body temperature and motion in real time, facilitating the early detection of infectious diseases. In this study, carried out within the framework of the European Union research project Rapidia Field, we tested the smart system on 10 pigs experimentally infected with two doses of an attenuated strain of African swine fever. Biosensors and an accelerometer embedded in an eartag captured data before and after infection, and video cameras were used to monitor the anima… Leer más
Martinez-Aviles M., Fernandez-Carrion E., Lopez Garcia-Baones JM. y Sanchez-Vizcaino JM..
A Comparison of Deformed Wing Virus in Deformed and Asymptomatic Honey Bees
Insects 8(1):1-12.
Deformed wing virus (DWV) in association with Varroa destructor is currently attributed to being responsible for colony collapse in the western honey bee (Apis mellifera). The appearance of deformed individuals within an infested colony has long been associated with colony losses. However, it is unknown why only a fraction of DWV positive bees develop deformed wings. This study concerns two small studies comparing deformed and non-deformed bees. In Brazil, asymptomatic bees (no wing deformity) that had been parasitised by Varroa as pupae had higher DWV loads than non-parasitised bees. However, we found no greater bilateral asymmetry in wing morphology due to DWV titres or parasitisation. As expected, using RT-qPCR, deformed bees were found … Leer más
Brettell LE., Mordecai GJ., Schroeder DC., Jones IM., de Silva JR., Vicente-Rubiano M. y Martin SJ.
Prediction of Pig Trade Movements in Different European Production Systems Using Exponential Random Graph Models
Frontiers In Veterinary Science 4(27):1-12.
In most European countries, data regarding movements of live animals are routinely collected and can greatly aid predictive epidemic modeling. However, the use of complete movements’ dataset to conduct policy-relevant predictions has been so far limited by the massive amount of data that have to be processed (e.g., in intensive commercial systems) or the restricted availability of timely and updated records on animal movements (e.g., in areas where small-scale or extensive production is predominant). The aim of this study was to use exponential random graph models (ERGMs) to reproduce, understand, and predict pig trade networks in different European production systems. Three trade networks were built by aggregating movements of pig batches … Leer más
Relun A., Grosbois V., Alexandrov T., Sanchez-Vizcaino JM., Waret-Szkuta A., Molia S., Charles-Etter EM. y Martinez-Lopez B.
Active animal health surveillance in European Union Member States: gaps and opportunities
Epidemiology And Infection 145(4):802-817.
Animal health surveillance enables the detection and control of animal diseases including zoonoses. Under the EU-FP7 project RISKSUR, a survey was conducted in 11 EU Member States and Switzerland to describe active surveillance components in 2011 managed by the public or private sector and identify gaps and opportunities. Information was collected about hazard, target population, geographical focus, legal obligation, management, surveillance design, risk-based sampling, and multi-hazard surveillance. Two countries were excluded due to incompleteness of data. Most of the 664 components targeted cattle (26·7%), pigs (17·5%) or poultry (16·0%). The most common surveillance objectives were demonstrating freedom from disease (43·8%) and case det… Leer más
Bisdorff B., Schauer B., Rodriguez-Prieto V., Comin A., Brouwer A., Dorea F., Drewe J., Hoinville L., Lindberg A., Martinez-Aviles M., Martinez-Lopez B., Peyre M., Pinto-Ferreira J., Rushton J., Van Schaik G., Stark KD., Staubach C., Vicente-Rubiano M., Witteveen G., Pfeiffer D. y Hasler B.
CHROMagar mSuperCARBA performance in carbapenem-resistant Enterobacteriaceae isolates characterized at molecular level and routine surveillance rectal swab specimens
Diagnostic Microbiology And Infectious Disease 87(3):207-209.
Performance of the CHROMagar mSuperCARBA media was assessed in both well-characterized carbapenem-resistant Enterobacteriaceae (n=52) and routine surveillance rectal swab specimens (n=211). Limit of detection ranged between 101 and 102CFU/mL except for OXA-48 producers with low-carbapenem MICs (106CFU/mL). High sensitivity (100%) and specificity (100%) were obtained with rectal swabs… Leer más
Garcia-Fernandez S., Hernandez M., Valverde A., Ruiz-Garbajosa P., Morosini MI. y Canton R.
Evaluation of five serologic assays for bovine tuberculosis surveillance in domestic free-range pigs from southern Spain
Preventive Veterinary Medicine 137(Pt A):101-104.
In countries where bovine tuberculosis (bTB) is still prevalent the contact among different animal species in extensive systems contributes to the circulation of Mycobacterium bovis (M. bovis) and other members of the Mycobacterium tuberculosis complex (MTC). Thus, free-range pigs can develop subclinical infections and may contribute to disease spread to bovine and wildlife. Serodiagnosis has been proposed as a screening tool for detecting infected pig herds; however, the value of this method to obtain an accurate diagnosis in this species is still not clear. In this study, sensitivity (Se) and specificity (Sp) estimates of four ELISAs and a lateral flow immunochromatographic antibody assay based on different M. bovis antigens, including MP… Leer más
Cardoso-Toset F., Luque I., Carrasco L., Jurado-Martos F., Risalde MA., Venteo A., Infantes-Lorenzo JA., Bezos J., Rueda P., Tapia I., Gortazar C., Dominguez L., Dominguez M. y Gomez-Laguna J.
Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
Research In Veterinary Science 110:34-39.
Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-α and to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of f… Leer más
Canal AM., Pezzone N., Cataldi A., Zumarraga M., Larzabal M., Garbaccio S., Fernandez A., Dominguez L., Aranaz A. y Rodriguez-Bertos A..
Effectiveness and practicality of control strategies for African swine fever: what do we really know?
The Veterinary Record 180(4):97.
African swine fever (ASF) is a major pig health problem, and the causative virus is moving closer to Western European regions where pig density is high. Stopping or slowing down the spread of ASF requires mitigation strategies that are both effective and practical. Based on the elicitation of ASF expert opinion, this study identified surveillance and intervention strategies for ASF that are perceived as the most effective by providing the best combination between effectiveness and practicality. Among the 20 surveillance strategies that were identified, passive surveillance of wild boar and syndromic surveillance of pig mortality were considered to be the most effective surveillance strategies for controlling ASF virus spread. Among the 22 i… Leer más
Guinat C., Vergne T., Jurado C., Sanchez-Vizcaino JM., Dixon L. y Pfeiffer DU.
Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs
PLoS ONE 12(1):e0170784.
The application of MALDI-TOF MS for identifying streptococcal isolates recovered from clinical specimens of diseased pigs was evaluated. For this proposal, the MALDI BDAL Database (Bruker Daltoniks, Germany) was supplemented with the main spectrum profiles (MSP) of the reference strains of S. porci, S. porcorum and S. plurextorum associated with pneumonia and septicemia. Although these three species showed similar MALDI profiles, several peaks were recognized that can be useful for their differentiation: S. porci (4113, 6133, 7975 and 8228 m/z Da), S. plurextorum (3979, 4078, 4665, 6164, 6491, 6812, 7959 and 9330 m/z Da) and S. porcorum (3385, 3954, 4190, 6772, 7908, and 8381 m/z Da). After adding these MSPs, an evaluation was conducted to … Leer más
Perez-Sancho M., Vela AI., Garcia-Seco T., Gonzalez S., Dominguez L. y Fernandez-Garayzabal JF..
A naturally occurring single nucleotide polymorphism in a multicopy plasmid produces a reversible increase in antibiotic resistance
Antimicrobial Agents And Chemotherapy 61(2):e01735-16.
ColE1 plasmids are small mobilizable replicons that play an important role in the spread of antibiotic resistance in Pasteurellaceae. In this study we describe how a natural single nucleotide polymorphism (SNP) near the origin of replication of the ColE1-type plasmid pB1000 found in a Pasteurella multocida clinical isolate, generates two independent plasmid variants able to coexist in the same cell simultaneously. Using Haemophilus influenzae Rd strain as a model system, we combined antibiotic susceptibility tests, quantitative PCRs, competition assays and experimental evolution to characterize the consequences of the coexistence of the pB1000 plasmid variants. This coexistence produced an increase of the total plasmid copy number (PCN) in … Leer más
Santos-Lopez A., Bernabe-Balas C., Ares M., Ortega-Huedo R., Hoefer A., San Millan A. y Gonzalez-Zorn B..
Canine Nervous System Lymphoma Subtypes Display Characteristic Neuroanatomical Patterns
Veterinary Pathology 54(1):53-60.
Primary and secondary nervous system involvement occurs in 4% and 5%-12%, respectively, of all canine non-Hodgkin lymphomas. The recent new classification of canine malignant lymphomas, based on the human World Health Organization classification, has been endorsed with international acceptance. This histological and immunocytochemical classification provides a unique opportunity to study the histologic anatomic distribution patterns in the central and peripheral nervous system of these defined lymphoma subtypes. In this study, we studied a cohort of 37 dogs with lymphoma, which at necropsy had either primary (n = 1, 2.7%) or secondary (n = 36; 97.3%) neural involvement. These T- (n = 16; 43.2%) or B-cell (n = 21; 56.8%) lymphomas were furth… Leer más
Siso S., Marco-Salazar P., Moore PF., Sturges BK., Vernau W., Wisner ER., Bollen AW., Dickinson PJ. y Higgins RJ.
