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Human influence and biotic homogenization drive the distribution of Escherichia coli virulence genes in natural habitats


1 de junio de 2017

Investigación publicada en MicrobiologyOpen


Cattle are the main reservoirs for Shiga-toxin-producing Escherichia coli (STEC), the only known zoonotic intestinal E. coli pathotype. However, there are other intestinal pathotypes that can cause disease in humans, whose presence has been seldom investigated. Thus, our aim was to identify the effects of anthropic pressure and of wild and domestic ungulate abundance on the distribution and diversity of the main human E. coli pathotypes and nine of their representative virulence genes (VGs). We used a quantitative real-time PCR (qPCR) for the direct detection and quantification of the genus-specific gene uidA, nine E. coli VGs (stx1, sxt2, eae, ehxA, aggR, est, elt, bfpA, invA), as well as four genes related to O157:H7 (rfbO157 , fliCH7 ) and O104:H4 (wzxO104 , fliCH4 ) serotypes in animals (feces from deer, cattle, and wild boar) and water samples collected in three areas of Doñana National Park (DNP), Spain. Eight of the nine VGs were detected, being invA, eae, and stx2 followed by stx1, aggR, and ehxA the most abundant ones. In quantitative terms (gene copies per mg of sample), stx1 and stx2 gave the highest values. Significant differences were seen regarding VGs in the three animal species in the three sampled areas. The serotype-related genes were found in all but one sample types. In general, VGs were more diverse and abundant in the northern part of the Park, where the surface waters are more contaminated by human waste and farms. In the current study, we demonstrated that human influence is more relevant than host species in shaping the E. coli VGs spatial pattern and diversity in DNP. In addition, wildlife could be potential reservoirs for other pathotypes different from STEC, however further isolation steps would be needed to completely characterize those E. coli




Cabal A., Vicente J., Alvarez J., Barasona JA., Boadella M., Dominguez L. y Gortazar C.




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Human influence and biotic homogenization drive the distribution of Escherichia coli virulence genes in natural habitats

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Human influence and biotic homogenization drive the distribution of Escherichia coli virulence genes in natural habitats

Participantes:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

University of MinnesotaDepartment of Veterinary Population Medicine. College of Veterinary Medicine. University of Minnesota (UMM).







MicrobiologyOpen
FACTOR YEAR Q
2.747 2016

NLMID: 101588314

PMID: 28213899

ISSN: 2045-8827



TÍTULO: Human influence and biotic homogenization drive the distribution of Escherichia coli virulence genes in natural habitats


REVISTA: Microbiologyopen


NUMERACIÓN: 6(3):1-10


AÑO: 2017


EDITORIAL: Oxford : Wiley-Blackwell


AUTORES: Cabal A., Vicente J., Alvarez J., Barasona JA., Boadella M., Dominguez L. and Gortazar C.


PARTICIPANTES VISAVET


4th
José Ángel Barasona García-Arévalo
6th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1002/mbo3.445


CITA ESTA PUBLICACIÓN:

Cabal A., Vicente J., Alvarez J., Barasona JA., Boadella M., Dominguez L. y Gortazar C. Human influence and biotic homogenization drive the distribution of Escherichia coli virulence genes in natural habitats. MicrobiologyOpen . 6(3):1-10. 2017. (A). ISSN: 2045-8827. DOI: 10.1002/mbo3.445


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