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Tuberculin skin testing boosts interferon-gamma responses to DIVA reagents in Mycobacterium bovis-infected cattle


5 de mayo de 2017

Clinical and Vaccine Immunology publica este artículo de investigación


BCG vaccination sensitises cattle to bovine tuberculin which compromises the use of the current bovine TB surveillance tests. Although the performance of a blood test (that utilises antigens expressed by Mycobacterium bovis but not by BCG) capable of discriminating infected from vaccinated animals (DIVA interferon-gamma test, DIT) has been evaluated in naturally infected TB field reactors, there is a need to perform similar analysis in a BCG vaccinated/M. bovis infected population. Furthermore, we explored different scenarios under which a DIT may be implemented alongside BCG vaccination: (i) serial testing to resolve potential false positive skin test results; or (ii) standalone test to replace the single intradermal comparative cervical tuberculin skin test (SICCT). Our results demonstrated significantly better relative test sensitivity when the DIT was evaluated in a serial test scenario. Direct comparison of pre and post skin test blood samples revealed that the SICCT test induced significant boosting of the interferon-gamma response in M. bovis-infected animals to both the ESAT-6/CFP-10 and Rv3615c peptide cocktails that comprise the DIT, which persisted for the ESAT-6/CFP-10 reagent for at least 14 days. Importantly, no similar boosting effects were observed in non-infected BCG vaccinates, suggesting that DIVA blood testing after a recent skin test would have minimal impact on test specificity




Jones GJ., Coad M., Khatri B., Bezos J., Parlane NA., Buddle BM., Villareal-Ramos B., Hewinson RG. y Vordermeier M.




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Tuberculin skin testing boosts interferon-gamma responses to DIVA reagents in Mycobacterium bovis-infected cattle

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Tuberculin skin testing boosts interferon-gamma responses to DIVA reagents in Mycobacterium bovis-infected cattle

Participantes:

Animal and Plant Health AgencyTB Immunology and Vaccinology. Department of Bacteriology. Animal and Plant Health Agency (APHA).

MAEVA SERVET, S.L.MAEVA SERVET, S.L..

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Hopkirk Research Institute. AgResearch. National Centre for Biosecurity and Infectious Disease (NCBID).







Clinical and Vaccine Immunology
FACTOR YEAR Q
2.425 2016

NLMID: 101252125

PMID: 28331078

ISSN: 1556-6811



TÍTULO: Tuberculin skin testing boosts interferon-gamma responses to DIVA reagents in Mycobacterium bovis-infected cattle


REVISTA: Clin. Vaccine Immunol


NUMERACIÓN: 24(5). pii: e00551-16


AÑO: 2017


EDITORIAL: American Society for Microbiology


AUTORES: Jones GJ., Coad M., Khatri B., Bezos J., Parlane NA., Buddle BM., Villareal-Ramos B., Hewinson RG. and Vordermeier M.


PARTICIPANTES VISAVET


Javier Bezos Garrido

DOI: https://doi.org/10.1128/CVI.00551-16


CITA ESTA PUBLICACIÓN:

Jones GJ., Coad M., Khatri B., Bezos J., Parlane NA., Buddle BM., Villareal-Ramos B., Hewinson RG. y Vordermeier M. Tuberculin skin testing boosts interferon-gamma responses to DIVA reagents in Mycobacterium bovis-infected cattle. Clinical and Vaccine Immunology . 24(5). pii: e00551-16. 2017. (A). ISSN: 1556-6811 . DOI: 10.1128/CVI.00551-16


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