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Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs


26 de enero de 2017

Artículo de investigación publicado en PLoS ONE


The application of MALDI-TOF MS for identifying streptococcal isolates recovered from clinical specimens of diseased pigs was evaluated. For this proposal, the MALDI BDAL Database (Bruker Daltoniks, Germany) was supplemented with the main spectrum profiles (MSP) of the reference strains of S. porci, S. porcorum and S. plurextorum associated with pneumonia and septicemia. Although these three species showed similar MALDI profiles, several peaks were recognized that can be useful for their differentiation: S. porci (4113, 6133, 7975 and 8228 m/z Da), S. plurextorum (3979, 4078, 4665, 6164, 6491, 6812, 7959 and 9330 m/z Da) and S. porcorum (3385, 3954, 4190, 6772, 7908, and 8381 m/z Da). After adding these MSPs, an evaluation was conducted to determine the accuracy of MALDI-TOF MS for the identification of streptococci from diseased pigs using 74 field isolates. Isolates were identified as S. suis, S. porcinus, S. dysgalactiae, S. hyovaginalis, S. porcorum, S. alactolyticus, S. hyointestinalis and S. orisratti. This is the first time that the latter three species have been reported from clinical specimens of pigs. Overall, there was good concordance (95.9%) between the results obtained from MALDI-TOF MS identification (best hint) and those from genotyping. Our results demonstrate the good performance of MALDI-TOF MS (100% sensitivity and specificity) for identifying most of the species of streptococci that can frequently be isolated from diseased pigs. However, conflicting results were observed in the correct identification of some isolates of S. dysgalactiae and S. alactolyticus




Perez-Sancho M., Vela AI., Garcia-Seco T., Gonzalez S., Dominguez L. y Fernandez-Garayzabal JF..




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Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs

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Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs

Participantes:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Campus MONCLOACampus MONCLOA.

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







PLoS ONE
FACTOR YEAR Q
2.806 2016

NLMID: 101285081

PMID: 28125697

ISSN: 1932-6203



TÍTULO: Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs


REVISTA: PLoS ONE


NUMERACIÓN: 12(1):1-10


AÑO: 2017


EDITORIAL: Public Library Science


AUTORES: Perez-Sancho M., Vela AI., Garcia-Seco T., Gonzalez S., Dominguez L. and Fernandez-Garayzabal JF..


PARTICIPANTES VISAVET


First
Marta Pérez Sancho
2nd
Ana Isabel Vela Alonso
3rd
Teresa García-Seco Romero
4th
Sergio González Domínguez
5th
Lucas Domínguez Rodríguez
Last
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.1371/journal.pone.0170784


CITA ESTA PUBLICACIÓN:

Perez-Sancho M., Vela AI., Garcia-Seco T., Gonzalez S., Dominguez L. y Fernandez-Garayzabal JF. Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs. PLoS ONE. 12(1):1-10. 2017. (A). ISSN: 1932-6203. DOI: 10.1371/journal.pone.0170784


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