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Antibody detection tests improve the sensitivity of tuberculosis diagnosis in cattle


1 de junio de 2017

Investigación publicada en Research in Veterinary Science


We evaluated the sensitivity (Se) of the single cervical intradermal tuberculin (SIT) test, two interferon-gamma (IFN-γ) assays and three different antibody detection techniques for bovine tuberculosis (bTB) diagnosis in 131 mixed beef breed cattle. The results of the diagnostic techniques performed over the whole herd, and over the animals confirmed as infected based on the presence of lesions compatible with the disease and/or M. bovis isolation were compared to determine apparent prevalence (AP) and Se.
The Se of the SIT test (severe interpretation) was 63.7% (95% CI, 54.54–72.00), while the Se of the IFN-γ assays ranged between 60.2% and 92%. The proportion of infected cattle detected by the different antibody detection techniques ranged from 65.5% to 87.6%. Three of the antibody detection techniques yielded a significant higher (p < 0.05) Se than that achieved with the official diagnostic techniques. In addition, the interpretation in parallel of cellular and antibody detection techniques reached the highest Se: 98.2% (95% CI, 93.78–99.51) suggesting that the use of diagnostic techniques detecting both cellular and humoral responses could be considered as an alternative in the control of bTB outbreaks in high prevalence settings




Casal C., Infantes-Lorenzo JA., Risalde MA., Diez-Guerrier A., Dominguez M., Moreno I., Romero B., de Juan L., Saez JL., Juste R., Gortazar C., Dominguez L. y Bezos J..




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Antibody detection tests improve the sensitivity of tuberculosis diagnosis in cattle

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Antibody detection tests improve the sensitivity of tuberculosis diagnosis in cattle

Participantes:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

MAEVA SERVET, S.L.MAEVA SERVET, S.L..

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Department for Environment, Food and Rural AffairsFood and Environment Research Agency (FERA). Department for Environment, Food and Rural Affairs (Defra).

Gobierno del Principado de AsturiasServicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA). Gobierno del Principado de Asturias.







Research in Veterinary Science
FACTOR YEAR Q
1.298 2016

NLMID: 401300

PMID: 28521256

ISSN: 0034-5288



TÍTULO: Antibody detection tests improve the sensitivity of tuberculosis diagnosis in cattle


REVISTA: Res. Vet. Sci.


NUMERACIÓN: 112(2017):214-221


AÑO: 2017


EDITORIAL: Elsevier


AUTORES: Casal C., Infantes-Lorenzo JA., Risalde MA., Diez-Guerrier A., Dominguez M., Moreno I., Romero B., de Juan L., Saez JL., Juste R., Gortazar C., Dominguez L. and Bezos J..


PARTICIPANTES VISAVET


2nd
Jose Antonio Infantes Lorenzo
4th
Alberto Antoine Díez Guerrier
7th
Beatriz Romero Martínez
8th
Lucía de Juan Ferré
12th
Lucas Domínguez Rodríguez
Last
Javier Bezos Garrido

DOI: http://dx.doi.org/10.1016/j.rvsc.2017.05.012


CITA ESTA PUBLICACIÓN:

Casal C., Infantes-Lorenzo JA., Risalde MA., Diez-Guerrier A., Dominguez M., Moreno I., Romero B., de Juan L., Saez JL., Juste R., Gortazar C., Dominguez L. y Bezos J. Antibody detection tests improve the sensitivity of tuberculosis diagnosis in cattle. Research in Veterinary Science. 112(2017):214-221. 2017. (A). ISSN: 0034-5288. DOI: 10.1016/j.rvsc.2017.05.012


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