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Differentiation of Flavobacterium psychrophilum from Flavobacterium psychrophilum-like species by MALDI-TOF mass spectrometry


1 de diciembre de 2017

Investigación publicada en Research in Veterinary Science


Rainbow trout fry syndrome (RTFS) is an important infectious disease caused by Flavobacterium psychrophilum affecting farmed salmonids worldwide. Other Flavobacterium psychrophilum-like species (F. plurextorum, F. oncorhynchi, F. tructae, F. collinsii and F. piscis) have been isolated from diseased rainbow trout fry suspected of RTFS although the epidemiological and clinical relevance of these pathogens are unknown. The objective of this study was to evaluate the potential use of MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time of Flight) Mass Spectrometry as method for specific identification of F. psychrophilum and its differentiation from other F. psychrophilum-like species isolated from diseased fish. Fifty-three isolates were analyzed after the creation of the Main Spectrum Profile (MSP) of reference strains of each of abovementioned species. F. psychrophilum exhibited a mass spectra very different from those of F. psychrophilum-like species, with five peaks (m/z 3654, 4585, 5388, 6730 and 7310) present only in F. psychrophilum isolates, and three peaks (m/z 6170, 7098 and 9241) absent in F. psychrophilum but present in all F. psychrophilum-like species. All F. psychrophilum isolates were correctly identified and differentiated from the F. psychrophilum-like species by MALDI-TOF. Although this approach showed a limited ability to differentiate among F. psychrophilum-like species, its complementation with a few simple biochemical tests may represent an alternative approach for the routine identification of the Flavobacterium psychrophilum-like species




Perez-Sancho M., Vela AI., Wiklund T., Kostrzewa M., Dominguez L. y Fernandez-Garayzabal JF..




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Differentiation of Flavobacterium psychrophilum from Flavobacterium psychrophilum-like species by MALDI-TOF mass spectrometry

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Differentiation of Flavobacterium psychrophilum from Flavobacterium psychrophilum-like species by MALDI-TOF mass spectrometry

Participantes:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Laboratory of Aquatic Pathobiology. Environmental and Marine Biology. Abo Akademi University.

Bruker Daltonik GmbHBruker Daltonik GmbH (BRUKER).







Research in Veterinary Science
FACTOR YEAR Q
1.298 2016

NLMID: 401300

PMID: 28688366

ISSN: 0034-5288



TÍTULO: Differentiation of Flavobacterium psychrophilum from Flavobacterium psychrophilum-like species by MALDI-TOF mass spectrometry


REVISTA: Res. Vet. Sci.


NUMERACIÓN: 115(2017):345-352


AÑO: 2017


EDITORIAL: Elsevier


AUTORES: Perez-Sancho M., Vela AI., Wiklund T., Kostrzewa M., Dominguez L. and Fernandez-Garayzabal JF..


PARTICIPANTES VISAVET


First
Marta Pérez Sancho
2nd
Ana Isabel Vela Alonso
5th
Lucas Domínguez Rodríguez
Last
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.1016/j.rvsc.2017.06.022


CITA ESTA PUBLICACIÓN:

Perez-Sancho M., Vela AI., Wiklund T., Kostrzewa M., Dominguez L. y Fernandez-Garayzabal JF. Differentiation of Flavobacterium psychrophilum from Flavobacterium psychrophilum-like species by MALDI-TOF mass spectrometry. Research in Veterinary Science. 115(2017):345-352. 2017. (A). ISSN: 0034-5288. DOI: 10.1016/j.rvsc.2017.06.022


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