Inicio \ Investigación \ Publicaciones científicas \


Time, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples

Veterinary Research Communications publica este artículo de investigación

1 de agosto de 2024

The isolation of Campylobacter fetus subsp. venerealis (Cfv) from clinical samples is the gold standard for confirming cases of bovine genital campylobacteriosis, an important cause of infertility in cattle and a potential public health concern. Furthermore, isolation is also necessary for the development of autologous vaccines, characterization of strains for antimicrobial susceptibility patterns, etc. Nevertheless, the sensitivity of culture methods is usually low, and there is no standardized protocol to maximize the recovery of Cfv from clinical samples. The aim of the current study is to design a protocol for the culture of Cfv from preputial samples by evaluating the combination of different transport, enrichment and culture media considering the impact of certain factors (time between collection and enrichment, temperature, and use of filters). The use of modified Lander`s transport medium and storing the sample for 24 h at 21 ± 2 °C led to the highest recovery of Cfv CFUs. In contrast, the storage of the samples during 24-48 h in PBS and Thomann rarely allowed the recovery of Cfv regardless of the temperature. The enrichment medium yielding the best results was Preston (significantly higher recovery than Brucella medium), while Cfv could not be isolated with Bolton. Regarding our diagnostic assay (using Lander as transport medium and Preston as enrichment medium), the best protocol in terms of maximizing Cfv recovery as well as limiting contaminations is to culture the samples in i) solid media Preston or Skirrow, and ii) using 0.65 μm filters and incubating plates at 37 °C in microaerophilic conditions




Polo C., Garcia-Seco T., Garcia N., Fernandez-Benito V., Briones V., Diez-Guerrier A., Alvarez J., Dominguez L. y Perez-Sancho M..




Ver artículo
Time, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples

Ver en NLM PubMed
Time, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples



Participantes:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Servicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

MAEVA SERVET, S.L.MAEVA SERVET, S.L..

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Zootecnia Análisis Clínicos VeterinariosZootecnia Análisis Clínicos Veterinarios.







Veterinary Research Communications
FACTOR YEAR Q
1.800 2023

NLMID: 8100520

PMID: 38598116

ISSN: 0165-7380



TÍTULO: Time, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples


REVISTA: Vet Res Commun


NUMERACIÓN: 48(4):2109-2119


AÑO: 2024


EDITORIAL: Springer


AUTORES: Polo C., Garcia-Seco T., Garcia N., Fernandez-Benito V., Briones V., Diez-Guerrier A., Alvarez J., Dominguez L. and Perez-Sancho M..


2nd
María Teresa García-Seco Romero
3rd
Nerea García Benzaquén
5th
Víctor Briones Dieste
6th
Alberto Antoine Díez Guerrier
7th
Julio Álvarez Sánchez
8th
Lucas Domínguez Rodríguez
Last
Marta Pérez Sancho

DOI: https://doi.org/10.1007/s11259-024-10362-8


CITA ESTA PUBLICACIÓN:

Polo C., Garcia-Seco T., Garcia N., Fernandez-Benito V., Briones V., Diez-Guerrier A., Alvarez J., Dominguez L. y Perez-Sancho M. Time, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples. Veterinary Research Communications. 48(4):2109-2119. 2024. (A). ISSN: 0165-7380. DOI: 10.1007/s11259-024-10362-8


SERVICIOS: