Publicaciones científicas SCI 2015
Efficacy of a typing scheme for campylobacter based on the combination of true and questionable CRISPR
Journal Of Microbiological Methods 119:147-53.
This study evaluates an improved scheme for Campylobacter genotyping based on the combination of true and questionable CRISPR (Clustered regularly interspaced short palindromic repeats) elements. A total of 180 Campylobacter strains (C. jejuni n = 93 and C. coli n = 87), isolated from neck skin and caecal content of broilers, poultry meat and sewage water were analysed. Another 97 C. jejuni DNA samples from cases of human campylobacteriosis were assessed. Sixty-three genotypes were found in C. jejuni considering only true CRISPR, and 16 additional genotypes were identified when questionable CRISPR were also taken into account. Likewise in C. coli the number of genotypes increased from eight for only true CRISPR to 14 after including questio… Leer más
de Cardenas I., Fernandez-Garayzabal JF., de la Cruz ML., Dominguez L., Ugarte-Ruiz M. y Gomez S.
Pelistega suis sp. nov., isolated from domestic and wild animals
International Journal Of Systematic And Evolutionary Microbiology 65(12):4909-14.
Biochemical and molecular genetic studies were performed on three novel Gram-stain-negative, catalase, oxidase-positive bacilli-shaped organisms isolated from tonsils of two pigs and one wild boar. The microorganism was identified as a species associated with the genus Pelistega based on its cellular morphological and biochemical tests. On the basis of 16S rRNA gene sequence similarities, isolates were most closely related to Pelistega indica (98.2%). groEL and gyrB sequence analysis showed interspecies divergence from its closest 16S rRNA phylogenetic relative, P. indica of 87.0% and 69%, respectively. The polyamine pattern contains predominantly putrescine and 2-hydroxyputrescine. The major quinone is ubiquinone Q-8 and in the polar lipid… Leer más
Vela AI., Perez-Sancho M., Dominguez L., Busse HJ. y Fernandez-Garayzabal JF..
First Detection of Antibodies Against African Swine Fever Virus in Faeces Samples
Transboundary And Emerging Diseases 62(6):594-602.
African swine fever (ASF) is a viral, highly lethal haemorrhagic disease of swine with no available vaccine or effective treatment. Introduction of ASF into a country triggers immediate restriction measures that cause significant economic losses and threatens spread to neighbouring countries. Wild boar populations have been recently assigned an essential role in the spread of African swine fever virus (ASFV) to European countries. Therefore, effective surveillance and monitoring of wild boar populations is required, but sampling wild boar is logistically challenging and expensive. This study assessed the feasibility of detecting antibodies against ASFV in faeces for later implementation in surveillance and control programmes. Two groups of … Leer más
Nieto-Pelegrin E., Rivera B. y Sanchez-Vizcaino JM..
Experimental Transmission of African Swine Fever (ASF) Low Virulent Isolate NH/P68 by Surviving Pigs
Transboundary And Emerging Diseases 62(6):612-22.
African swine fever (ASF) has persisted in Eastern Europe since 2007, and two endemic zones have been identified in the central and southern parts of the Russian Federation. Moderate- to low-virulent ASF virus isolates are known to circulate in endemic ASF-affected regions. To improve our knowledge of virus transmission in animals recovered from ASF virus infection, an experimental in vivo study was carried out. Four domestic pigs were inoculated with the NH/P68 ASF virus, previously characterized to develop a chronic form of ASF. Two additional in-contact pigs were introduced at 72 days post-inoculation (dpi) in the same box for virus exposure. The inoculated pigs developed a mild form of the disease, and the virus was isolated from tissue… Leer más
Gallardo C., Soler A., Nieto R., Sanchez MA., Martins C., Pelayo V., Carrascosa AL., Revilla Y., Simon A., Briones V., Sanchez-Vizcaino JM. y Arias M.
Evaluation of the immunogenicity and diagnostic interference caused by M. tuberculosis SO2 vaccination against tuberculosis in goats
Research In Veterinary Science 103:73-79.
The immunogenicity and diagnostic interference caused by M. tuberculosis SO2, a prototype vaccine first time tested in goats was evaluated. Tuberculosis-free goats were distributed in four groups: [1], non-vaccinated; [2], subcutaneously (SC) BCG vaccinated; [3], intranasally (IN) SO2 vaccinated and [4], SC SO2 vaccinated. Intradermal tuberculin and IFN-γ tests using PPDs and alternative antigenic cocktails containing mainly ESAT-6 and CFP-10 (E/C) were applied at different times post-vaccination. Results showed a significant (p < 0.05) increase in the number of reactors detected using both PPD-based intradermal and IFN-γ tests at different times in all the vaccinated groups. No intradermal reactivity was detected in the vaccinate… Leer más
Bezos J., Casal C., Puentes E., Diez-Guerrier A., Romero B., Aguilo N., de Juan L., Martin C. y Dominguez L..
Detection of mycobacterial DNA by a specific and simple lateral flow assay incorporating cadmium selenide quantum dots
Molecular And Cellular Probes 29(6):534-6.
Cadmium selenide quantum dots have been incorporated to a lateral flow assay for the specific and very simple detection of different mycobacterial DNA targets within only a few minutes, bypassing the complexity of conventional DNA hybridization assays. The method extends our previous work on protein detection using an identical procedure… Leer más
Cimaglia F., Liandris E., Gazouli M., Sechi L., Chiesa M., De Lorenzis E., Margarita A., Taka S., Mataraqka A. y Ikonomopoulos J.
Comparative Genomics of Field Isolates of Mycobacterium bovis and M. caprae Provides Evidence for Possible Correlates with Bacterial Viability and Virulence
PLoS Neglected Tropical Diseases 9(11):e0004232.
Mycobacteria of the Mycobacterium tuberculosis complex (MTBC) greatly affect humans and animals worldwide. The life cycle of mycobacteria is complex and the mechanisms resulting in pathogen infection and survival in host cells are not fully understood. Recently, comparative genomics analyses have provided new insights into the evolution and adaptation of the MTBC to survive inside the host. However, most of this information has been obtained using M. tuberculosis but not other members of the MTBC such as M. bovis and M. caprae. In this study, the genome of three M. bovis (MB1, MB3, MB4) and one M. caprae (MB2) field isolates with different lesion score, prevalence and host distribution phenotypes were sequenced. Genome sequence information … Leer más
de la Fuente J., Diez-Delgado I., Contreras M., Vicente J., Cabezas-Cruz A., Tobes R., Manrique M., Lopez V., Romero B., Bezos J., Dominguez L., Sevilla I., Garrido JM., Juste R., Madico G., Jones-Lopez E. y Gortazar C.
First molecular determination of herpesvirus from two mysticete species stranded in the Mediterranean Sea
BMC Veterinary Research 11:283.
Background
Herpesvirus can infect a wide range of animal species: mammals, birds, reptiles, fish, amphibians and bivalves. In marine mammals, several alpha- and gammaherpesvirus have been identified in some cetaceans and pinnipeds species. To date, however, this virus has not been detected in any member of the Balaenoptera genus.
Case presentation
Herpesvirus was determined by molecular methods in tissue samples from a male fin whale juvenile (Balaenoptera physalus) and a female common minke whale calf (Balaenoptera acutorostrata) stranded on the Mediterranean coast of the Region of Valencia (Spain). Samples of skin and penile mucosa from the fin whale and samples of skin, muscle and central nervous system tissue from … Leer más
Melero M., Crespo JL., Rubio-Guerri C., Garcia-Parraga D. y Sanchez-Vizcaino JM..
RmtC and RmtF 16S rRNA Methyltransferase in NDM-1-Producing Pseudomonas aeruginosa
Emerging Infectious Diseases 21(11):2059-62.
We investigated 16S rRNA methyltransferases in 38 blaNDM-1-positive Pseudomonas aeruginosa isolates and found RmtC in 3 isolates, 1 of which also harbored RmtF. The isolates were clonally unrelated; rmtC and rmtF genes were located on a chromosome with the blaNDM-1 gene. Strategies are needed to limit the spread of such isolates… Leer más
Rahman M., Prasad KN., Pathak A., Pati BK., Singh A., Ovejero CM., Ahmad S. y Gonzalez-Zorn B..
Prevalence of Type VI Secretion System in Spanish Campylobacter jejuni Isolates
Zoonoses And Public Health 62(7):497-500.
Infections from Campylobacter jejuni pose a serious public health problem and are now considered the leading cause of foodborne bacterial gastroenteritis throughout the world. Sequencing of C. jejuni genomes has previously allowed a number of loci to be identified, which encode virulence factors that aid survival
and pathogenicity. Recently, a Type VI secretion system (T6SS) consisting of 13 conserved genes was described in C. jejuni strains and recognised to promote pathogenicity and adaptation to the environment. In this study, we determined the presence of this T6SS in 63 Spanish C. jejuni isolates from the food chain and
urban effluents using whole-genome sequencing. Our findings demonstrated that nine (14%) strains harbou… Leer más
Ugarte-Ruiz M., Stabler RA., Dominguez L., Porrero MC., Wren BW., Dorrell N. y Gundogdu O.
Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures
Veterinary Immunology And Immunopathology 167(3-4):185-9.
Tuberculosis (TB) in goats (Capra hircus) is due to infection with members of the Mycobacterium tuberculosis complex (MTC), mainly Mycobacterium bovis and M. caprae. We report a comparative experimental infection of goats with M. bovis, M. caprae and M. tuberculosis strains. We hypothesized that goats experimentally infected with different members of the MTC would display different clinical pictures. Three groups of goats were challenged with either M. bovis SB0134 (group 1, n = 5), M. caprae SB0157 (group 2, n = 5) and M. tuberculosis SIT58 (group 3, n = 4). The highest mean total lesion score was observed in M. bovis challenged goats (mean 15.2, range 9-19), followed by those challenged with M. caprae (10.8, 2-23). The lowest score was re… Leer más
Bezos J., Casal C., Diez-Delgado I., Romero B., Liandris E., Alvarez J., Sevilla I., de Juan L., Dominguez L. y Gortazar C.
Direct Detection of Escherichia coli Virulence Genes by Real-Time PCR in Fecal Samples from Bats in Brazil
Journal Of Wildlife Diseases 51(4):942-945.
Guano samples from 412 Brazilian bats were screened with real-time PCR for the virulence genes (eae, est, elt, stx1, stx2, ehxA, invA, bfpA, aggR) representing five intestinal pathotypes of Escherichia coli. From 82 pooled samples, 22% contained Escherichia coli DNA, and eae, est, bfpA, aggR were detected… Leer más
Cabal A., Pereira MJ., Aguilar LM., Dominguez L., Fonseca C., Alvarez J., Drexler JL. y Gortazar C.
Dietary vitamin A restriction affects adipocyte differentiation and fatty acid composition of intramuscular fat in Iberian pigs
Meat Science 108:9-16.
The aim of this study was to investigate whether dietary vitamin A level is associated with differences in adipocyte differentiation or lipid accumulation in Iberian pigs at early growing (35.8kg live weight) and at finishing (158kg live weight). Iberian pigs of 16.3kg live weight were allocated to two feeding groups, one group received 10,000IU of vitamin A/kg diet (control); the other group received a diet with 0IU of vitamin A (var) for the whole experimental period. The dietary vitamin A level had no effect on growth performance and carcass traits. The early suppression of vitamin A increased the preadipocyte number in Longissimus thoracis (LT) muscle in the early growth period (P<0.001) and the neutral lipid content and composition (hi… Leer más
Ayuso M., Ovilo C., Rodriguez-Bertos A., Rey AI., Daza A., Fernandez A., Gonzalez-Bulnes A., Lopez-Bote CJ. y Isabel B.
Multi-Etiological Nature of Tuberculosis-Like Lesions in Condemned Pigs at the Slaughterhouse
PLoS ONE 10(9):e0139130.
Tuberculosis-like lesions (TBL) in pigs have been associated with microorganisms other than mycobacteria. In this work a histopathological and microbiological evaluation of TBL in pigs is shown. A total of 352 samples belonging to 171 pigs totally condemned at slaughterhouse due to generalized TBL were sampled and selected for analysis. Pyogranulomatous (56.2%) and granulomatous lesions (20.2%) were observed in all analysed organs. Most of the granulomas observed in both lymph nodes and lungs belonged to more advanced stages of development (stages III and IV) whereas in the liver and the spleen most of lesions belonged to intermediate stages (stages II and III). Different microorganisms were simultaneously detected from TBL in the 42.7% of … Leer más
Cardoso-Toset F., Gomez-Laguna J., Amarilla SP., Vela AI., Carrasco L., Fernandez-Garayzabal JF., Astorga R. y Luque I.
Salmonella Typhimurium exploits inflammation to its own advantage in piglets
Frontiers In Microbiology 6:985.
Salmonella Typhimurium (S. Typhimurium) is responsible for foodborne zoonotic infections that, in humans, induce self-limiting gastroenteritis. The aim of this study was to evaluate whether the wild-type strain S. Typhimurium (STM14028) is able to exploit inflammation fostering an active infection. Due to the similarity between human and porcine diseases induced by S. Typhimurium, we used piglets as a model for salmonellosis and gastrointestinal research. This study showed that STM14028 is able to efficiently colonize in vitro porcine mono-macrophages and intestinal columnar epithelial (IPEC-J2) cells, and that the colonization significantly increases with LPS pre-treatment. This increase was then reversed by inhibiting the LPS stimulation … Leer más
Chirullo B., Pesciarioli M., Drumo R., Ruggeri J., Razzouli E., Pistoia C., Petrucci P., Martinelli N., Cucco L., Moscati L., Amadori M., Magistrali CF., Alborali GL. y Pasquali P.
Fatal Erysipelothrix rhusiopathiae septicemia in two Atlantic dolphins (Stenella frontalis and Tursiops truncatus)
Diseases Of Aquatic Organisms 116(1):75-81.
We describe gross, histopathologic, ultrastructural, immunohistochemical, and microbiologic features of acute septicemia by Erysipelothrix rhusiopathiae in an Atlantic spotted dolphin Stenella frontalis and an Atlantic bottlenose dolphin Tursiops truncatus. Generalized lymphadenomegaly and widespread hemorrhages were the most consistent macroscopic findings. Tricavitary effusion and icterus were noted in one individual. Histologically, all organs examined showed numerous variably sized bacillary bacterial emboli (Gram-positive; Ziehl-Neelsen-negative), typically associated with systemic congestion, edema, hemorrhages, and fibrinocellular thrombi. These bacteria were frequently intravascular, either extracellular or intramonocytic/macrophagi… Leer más
Diaz-Delgado J., Arbelo M., Sierra E., Vela AI., Dominguez M., Paz Y., Andrada M., Dominguez L. y Fernandez A.
Thermal reference points as an index for monitoring body temperature in marine mammals
BMC Research Notes 8(1):411.
BACKGROUND:
Monitoring body temperature is essential in veterinary care as minor variations may indicate dysfunction. Rectal temperature is widely used as a proxy for body temperature, but measuring it requires special equipment, training or restraining, and it potentially stresses animals. Infrared thermography is an alternative that reduces handling stress, is safer for technicians and works well for untrained animals. This study analysed thermal reference points in five marine mammal species: bottlenose dolphin (Tursiops truncatus); beluga whale (Delphinapterus leucas); Patagonian sea lion (Otaria flavescens); harbour seal (Phoca vitulina); and Pacific walrus (Odobenus rosmarus divergens).
RESULTS:
The thermogram ana… Leer más
Melero M., Rodriguez-Prieto V., Rubio-Garcia A., Garcia-Parraga D. y Sanchez-Vizcaino JM..
Use of colistin-containing products within the European Union and European Economic Area (EU/EEA): development of resistance in animals and possible impact on human and animal health
International Journal Of Antimicrobial Agents 46(3):297-306.
Since its introduction in the 1950s, colistin has been used mainly as a topical treatment in human medicine owing to its toxicity when given systemically. Sixty years later, colistin is being used as a last-resort drug to treat infections caused by multidrug-resistant (MDR) Pseudomonas aeruginosa, Acinetobacter baumannii and Enterobacteriaceae (e.g., Escherichia coli, Klebsiella pneumoniae), for which mortality can be high. In veterinary medicine, colistin has been used for decades for the treatment and prevention of infectious diseases. Colistin has been administered frequently as a group treatment for animal gastrointestinal infections caused by Gram-negative bacteria within intensive husbandry systems. Given the ever-growing need to reta… Leer más
Catry B., Cavaleri M., Baptiste K., Grave K., Grein K., Holm A., Jukes H., Liebana E., Navas AL., Mackay D., Magiorakos AP., Moreno MA., Moulin G., Madero CM., Pomba MC., Powell M., Pyorala S., Rantala M., Ruzauskas M., Sanders P., Teale C., Threlfall EJ., Torneke K., van Duijkeren E. y Edo JT.
Effect of the inoculation site of bovine purified protein derivative (PPD) on the skin fold thickness increase in cattle from officially tuberculosis free and tuberculosis-infected herds
Preventive Veterinary Medicine 121(1-2):86-92.
The official technique for diagnosis of bovine tuberculosis (bTB) worldwide is the tuberculin skin test, based on the evaluation of the skin thickness increase after the intradermal inoculation of a purified protein derivative (PPD) in cattle. A number of studies performed on experimentally infected or sensitized cattle have suggested that the relative sensitivity of the cervical test (performed in the neck) may vary depending on the exact location in which the PPD is injected. However, quantitative evidence on the variation of the test accuracy associated to changes in the site of inoculation in naturally infected animals (the population in which performance of the test is most critical for disease eradication) is lacking. Here, the probab… Leer más
Casal C., Alvarez J., Bezos J., Quick H., Diez-Guerrier A., Romero B., Saez-Llorente JL., Liandris E., Navarro A., Perez AM., Dominguez L. y de Juan L..
Testing Eurasian wild boar piglets for serum antibodies against Mycobacterium bovis
Preventive Veterinary Medicine 121(1-2):93-98.
Animal tuberculosis (TB) caused by infection with Mycobacterium bovis and closely related members of the M. tuberculosis complex (MTC), is often reported in the Eurasian wild boar (Sus scrofa). Tests detecting antibodies against MTC antigens are valuable tools for TB monitoring and control in suids. However, only limited knowledge exists on serology test performance in 2-6 month-old piglets. In this age-class, recent infections might cause lower antibody levels and lower test sensitivity. We examined 126 wild boar piglets from a TB-endemic site using 6 antibody detection tests in order to assess test performance. Bacterial culture (n=53) yielded a M. bovis infection prevalence of 33.9%, while serum antibody prevalence estimated by different… Leer más
Che Amat A., Gonzalez-Barrio D., Ortiz JA., Diez-Delgado I., Boadella M., Barasona JA., Bezos J., Romero B., Armenteros JA., Lyashchenko KP., Venteo A., Rueda P. y Gortazar C.
Streptococcus pharyngis sp. nov., a new streptococcal species isolated from the respiratory tract of wild rabbits
International Journal Of Systematic And Evolutionary Microbiology 65(9):2903-7.
Four isolates of an unknown Gram-positive, catalase-negative coccus-shaped organism isolated from pharynx of four wild rabbits, were characterized by phenotypic and molecular genetic methods. The microorganisms were tentatively assigned to the genus Streptococcus based on their cellular morphological and biochemical criteria, although the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequencing confirmed their identification as members of the genus Streptococcus, being phylogenetically closet related to Streptococcus porcorum 682-03T (96.9 % 16S rRNA gene sequence similarity). The rpoB and sodA sequence analysis showed divergence values between the novel isolate and S. porcorum 682-03T, the clos… Leer más
Vela AI., Casas-Diaz E., Lavin S., Dominguez L. y Fernandez-Garayzabal JF..
Livestock-Associated Methicillin Resistant and Methicillin Susceptible Staphylococcus aureus Sequence Type (CC)1 in European Farmed Animals: High Genetic Relatedness of Isolates from Italian Cattle Herds and Humans
PLoS ONE 10(8):e0137143.
Methicillin-resistant Staphylococcus aureus (MRSA) Sequence Type (ST)1, Clonal Complex(CC)1, SCCmec V is one of the major Livestock-Associated (LA-) lineages in pig farming industry in Italy and is associated with pigs in other European countries. Recently, it has been increasingly detected in Italian dairy cattle herds. The aim of this study was to analyse the differences between ST1 MRSA and methicillin-susceptible S. aureus (MSSA) from cattle and pig herds in Italy and Europe and human isolates. Sixty-tree animal isolates from different holdings and 20 human isolates were characterized by pulsed-field gel electrophoresis (PFGE), spa-typing, SCCmec typing, and by micro-array analysis for several virulence, antimicrobial resistance, and st… Leer más
Alba P., Feltrin F., Cordaro G., Porrero MC., Kraushaar B., Arqudin MA., Nykasenoja S., Monaco M., Stegger M., Aarestrup FM., Butaye P., Franco A. y Battisti A.
Assessment of MALDI-TOF MS as alternative tool for Streptococcus suis identification
Frontiers In Public Health 3:202.
The accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identifying Streptococcus suis isolates obtained from pigs, wild animals, and humans was evaluated using a PCR-based identification assay as the gold standard. In addition, MALDI-TOF MS was compared with the commercial multi-tests Rapid ID 32 STREP system. From the 129 S. suis isolates included in the study and identified by the molecular method, only 31 isolates (24.03%) had score values ≥2.300 and 79 isolates (61.24%) gave score values between 2.299 and 2.000. After updating the currently available S. suis MALDI Biotyper database with the spectra of three additional clinical isolates of serotypes 2, 7, and 9, most isolates… Leer más
Perez-Sancho M., Vela AI., Garcia-Seco T., Gottschalk M., Dominguez L. y Fernandez-Garayzabal JF..
A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus
BMC Veterinary Research 11:203.
Elephants are classified as critically endangered animals by the International Union for Conservation of Species (IUCN). Elephant endotheliotropic herpesvirus (EEHV) poses a large threat to breeding programs of captive Asian elephants by causing fatal haemorrhagic disease. EEHV infection is detected by PCR in samples from both clinically ill and asymptomatic elephants with an active infection, whereas latent carriers can be distinguished exclusively via serological assays. To date, identification of latent carriers has been challenging, since there are no serological assays capable of detecting seropositive elephants.
Here we describe a novel ELISA that specifically detects EEHV antibodies circulating in Asian elephant plasma… Leer más
van del Doel PB., Rodriguez-Prieto V., van Rossum-Fikkert SE., Schaftenaar W., Latimer E., Howard L., Chapman S., Masters N., Osterhaus AD., Linq PD., Dastjerdi A. y Martina B.
New insights into the role of ticks in African swine fever epidemiology
Revue Scientifique Et Technique 34(2):503-511.
African swine fever (ASF), one of the most important diseases of swine, is present in many African countries, as well as in eastern Europe, Russia and Sardinia. It is caused by a complex virus, ASF virus (ASFV), for which neither vaccine nortreatment is available. ASFV affects swine of all breeds and ages, and also replicates in soft ticks of the genus Ornithodoros, facilitating ASFV persistence
and reocurrence of disease. Depending on the involvement of these ticks, and the presence or not of sylvatic asymptomatic animals, several epidemiological cycles have been identified. The disease persists in East and southern African countries in a sylvatic cycle between O. porcinus (of the O. moubata species complex) and common warthogs. In … Leer más
Sanchez-Vizcaino JM., Mur L., Bastos ADS. y Penrith ML.
Detection of virulence-associated genes characteristic of intestinal Escherichia coli pathotypes, including the Enterohemorrhagic / Enteroaggregative O104:H4 in bovines from Germany and Spain
Microbiology And Immunology 59(8):433-42.
Cattle are reservoirs of Enterohemorrhagic Escherichia coli but their role in the epidemiology of other pathogenic E. coli remains undefined. We describe a new set of quantitative real-time PCR assays for the direct detection and quantification of nine virulence associated genes (VAGs) characteristic of the most important human E. coli pathotypes and four serotype-related genes (wzxO104 , fliCH4 , rbfO157 , fliCH7 ) that can be used as a surveillance tool for detection of pathogenic strains. A total of 970 cattle fecal samples were collected in slaughterhouses from Germany and Spain and 134 pooled samples and analyzed with this tool. Results showed that stx1, eae, and invA were more prevalent in Spanish samples while bfpA, stx2, ehxA, elt, … Leer más
Cabal A., Geue L., Gomez S., Barth S., Barcena C., Hamm K., Porrero MC., Valverde A., Canton R., Menge C., Gortazar C., Dominguez L. y Alvarez J..
Distinguishing Intestinal Lymphoma From Inflammatory Bowel Disease in Canine Duodenal Endoscopic Biopsy Samples
Veterinary Pathology 52(4):668-75.
Inflammatory bowel disease (IBD) and intestinal lymphoma are intestinal disorders in dogs, both causing similar chronic digestive signs, although with a different prognosis and different treatment requirements. Differentiation between these 2 conditions is based on histopathologic evaluation of intestinal biopsies. However, an accurate diagnosis is often difficult based on histology alone, especially when only endoscopic biopsies are available to differentiate IBD from enteropathy-associated T-cell lymphoma (EATL) type 2, a small cell lymphoma. The purpose of this study was to evaluate the utility of histopathology; immunohistochemistry (IHC) for CD3, CD20, and Ki-67; and polymerase chain reaction (PCR) for antigen receptor rearrangement (T… Leer más
Carrasco V., Rodriguez-Bertos A., Rodriguez-Franco F., Wise AG., Maes R., Mullaney T. y Kiupel M.
Systematic review of surveillance systems and methods for early detection of exotic, new and re-emerging diseases in animal populations
Epidemiology And Infection 143(10):2018-42.
In this globalized world, the spread of new, exotic and re-emerging diseases has become one of the most important threats to animal production and public health. This systematic review analyses conventional and novel early detection methods applied to surveillance. In all, 125 scientific documents were considered for this study. Exotic (n = 49) and re-emerging (n = 27) diseases constituted the most frequently represented health threats. In addition, the majority of studies were related to zoonoses (n = 66). The approaches found in the review could be divided in surveillance modalities, both active (n = 23) and passive (n = 5); and tools and methodologies that support surveillance activities (n = 57). Combinations of surveillance modalities … Leer más
Rodriguez-Prieto V., Vicente-Rubiano M., Sanchez-Matamoros A., Rubio-Guerri C., Melero M., Martinez-Lopez B., Martinez-Aviles M., Hoinville L., Vergne T., Comin A., Schauer B., Dorea F., Pfeiffer DU. y Sanchez-Vizcaino JM..
Complete Genome Sequences of Field Isolates of Mycobacterium bovis and Mycobacterium caprae
Genome Announcements 3(3):e00247-15.
Here we report the complete genome sequences of field isolates of Mycobacterium bovis and the related mycobacterial species, Mycobacterium caprae. The genomes of three M. bovis (MB1, MB3, MB4) and one M. caprae (MB2) field isolates with different virulence, prevalence, and host distribution phenotypes were sequenced… Leer más
de la Fuente J., Diez-Delgado I., Contreras M., Vicente J., Cabezas-Cruz A., Manrique M., Tobes R., Lopez V., Romero B., Dominguez L., Garrido JM., Juste R. y Gortazar C.
First Stranding Event of a Common Minke Whale Calf, Balaenoptera acutorostrata Lacépède, 1804, Reported in Spanish Mediterranean Waters
Mammal Study 40(2):95-100.
At least six mysticete species have been reported to date in the Mediterranean Sea (Notarbartolo di Sciara and Birkun 2010). The fin whale, Balaenoptera physalus, is the only mysticete species with a stable population in the Mediterranean Sea, which differs genetically from Atlantic populations (Giménez et al. 2013 and references therein). Other mysticete species have been previously recorded as either sighted or stranded in this area (Notarbartolo di Sciara and Birkun 2010). The humpback whale, Megaptera novaeangliae, and the common minke whale, B. acutorostrata, are considered rare visitors to the Mediterranean, with less than a one sighting or stranded record per species, per year (IUCN 2012). The sei whale, B. borealis, the North Atlant… Leer más
Fraija-Fernandez N., Crespo JL., Domenech F., Míguez-Lozano R., Palacios-Abella JF., Rodriguez-Gonzalez A., Villar-Torres M. y Gozalbes P.
Lack of interference with diagnostic testing for tuberculosis in goats experimentally exposed to Corynebacterium pseudotuberculosis
The Veterinary Journal 205(1):113-5.
It has been suggested that infection with Corynebacterium pseudotuberculosis, the pathogen responsible for caseous lymphadenitis (CLA), might interfere with diagnostic testing for tuberculosis (TB), due to antigenic similarities between this particular type of bacterium and those expressed by mycobacteria. The aim of the present study was to investigate whether experimental infection with C. pseudotuberculosis in goats impacted on TB testing, using single and comparative intradermal tuberculin (SIT and SCIT respectively) tests and interferon (IFN)-γ assay. No positive reactors were detected among the CLAaffected goats using the SIT/SCIT tests or the interferon IFN-γ assay. A proportion of goats showed inconclusive results to the S… Leer más
Bezos J., Casal C., Romero B., Liandris E., Sanchez-Coppel N., Vigo V., Dominguez L. y de Juan L..
Small-Plasmid-Mediated Antibiotic Resistance Is Enhanced by Increases in Plasmid Copy Number and Bacterial Fitness
Antimicrobial Agents And Chemotherapy 59(6):3335-41.
Plasmids play a key role in the horizontal spread of antibiotic resistance determinants among bacterial pathogens. When an antibiotic-resistance plasmid arrives to a new bacterial host it produces a fitness cost, causing a competitive disadvantage for the plasmid-bearing bacterium in the absence of antibiotics. On the other hand, in the presence of antibiotics the plasmid promotes the survival of the clone. The adaptations experienced by plasmid and bacteria in the presence of antibiotics during the first generations of coexistence will be crucial for the progress of the infection and the maintenance of plasmid-mediated resistance once the treatment is over. Here we developed a model system using the human pathogen Haemophilus influenzae ca… Leer más
San Millan A., Santos-Lopez A., Ortega-Huedo R., Bernabe-Balas C., Kennedy SP. y Gonzalez-Zorn B..
Escherichia coli O157:H7 in wild boars (Sus scrofa) and Iberian ibex (Capra pyrenaica) sharing pastures with free-ranging livestock in a natural environment in Spain
The Veterinary Quarterly 35(2):102-6.
Background: Wild ungulates have greatly increased in abundance and range throughout Europe. This new situation presents a concern for public health because many wild ungulates are known reservoirs of zoonotic pathogens.
Objectives: In this work, we tested for the presence of the zoonotic pathogen Escherichia coli O157:H7 in free-ranging livestock and sympatric wild boars (Sus scrofa) and Iberian ibex (Capra pyrenaica) in NE Spain from 2009 to 2011. In addition, antimicrobial resistance and virulence factors were assessed.
Animals and methods: In total, individual fecal samples were obtained from 117 hunter-harvested wild boars and 160 Iberian ibexes. Fifty-five samples were obtained from cattle (5 herds, 380 animals in total) … Leer más
Navarro-Gonzalez N., Porrero MC., Mentaberre G., Serrano E., Mateos A., Cabal A., Dominguez L. y Lavin S.
Assessing the risk of African swine fever introduction into the European Union by wild boar
Transboundary And Emerging Diseases 62(3):272-9.
The presence of African swine fever (ASF) in the Caucasus region and Russian Federation has increased concerns that wild boars may introduce the ASF virus into the European Union (EU). This study describes a semi-quantitative approach for evaluating the risk of ASF introduction into the EU by wild boar movements based on the following risk estimators: the susceptible population of (1) wild boars and (2) domestic pigs in the country of origin; the outbreak density in (3) wild boars and (4) domestic pigs in the countries of origin, the (5) suitable habitat for wild boars along the EU border; and the distance between the EU border and the nearest ASF outbreak in (6) wild boars or (7) domestic pigs. Sensitivity analysis was performed to identif… Leer más
de la Torre A., Bosch J., Iglesias I., Munoz MJ., Mur L., Martinez-Lopez B., Martinez-Aviles M. y Sanchez-Vizcaino JM..
Comparative analysis of cellular immune responses and cytokine levels in sheep experimentally infected with bluetongue virus serotype 1 and 8
Veterinary Microbiology 177(1-2):95-105.
Protective immunity in sheep with bluetongue virus (BTV) infection as well as the role of BTV-induced cytokines during immune response remains unclear. Understanding the basis immunological mechanisms in sheep experimentally infected with serotypes 1 and 8 (BTV-1 and -8) was the aim of this study. A time-course study was carried out in order to evaluate cell-mediated immune response and serum concentrations of cytokines (IL-1β, TNFα, IL-12, IFNγ, IL-4 and IL-10) with inflammatory and immunological functions. Depletion of T cell subsets (mainly CD4+, γδ and CD25+) together with the absence of cytokines (IFNγ and IL-12) involved in the regulation of cell-mediated antiviral immunity at the first stage of the disea… Leer más
Sanchez-Cordon PJ., Perez de Diego AC., Gomez-Villamandos JC., Sanchez-Vizcaino JM., Pleguezuelos FJ., Garfia B., del Carmen P. y Pedrera M.
Novel blaROB-1-Bearing Plasmid Conferring Resistance to B-Lactams in Haemophilus parasuis Isolates from Healthy Weaning Pigs
Applied And Environmental Microbiology 81(9):3255-67.
Haemophilus parasuis, the causative agent of Glässer`s disease, is one of the early colonizers of the nasal mucosa of piglets. It is prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are commonly used in disease control, and resistance to several antibiotics has been described in H. parasuis. Prediction of H. parasuis virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with H. parasuis virulence, such as lsgB and group 1 vtaA, while biofilm growth has been associated to non-virulent strains. In this study, 86 H. parasuis nasal isolates from farms without disease for more than 10 years were obtained by sampli… Leer más
Moleres J., Santos-Lopez A., Lazaro I., Labairu J., Prat C., Ardanuy C., Gonzalez-Zorn B., Aragon V. y Garmendia J.
Antibiotic-resistant Klebsiella pneumoniae and Escherichia coli high-risk clones and an IncFII(k) mosaic plasmid hosting Tn1 (blaTEM-4) in isolates from 1990 to 2004
Antimicrobial Agents And Chemotherapy 59(5):2904-8.
We describe the genetic background of blaTEM-4 and the complete sequence of pRYC11::blaTEM-4, a mosaic plasmid highly similar to pKpQIL-like variants, predominant among TEM-4 producers in a Spanish hospital (1990-2004) which belong to Klebsiella pneumoniae and Escherichia coli high-risk clones responsible for the current spread of different antibiotic resistance genes. Predominant populations of plasmids and host adapted clonal lineages seem to have greatly contributed to the spread of resistance to extended-spectrum cephalosporins… Leer más
Rodriguez I., Novais A., Lira F., Valverde A., Curiao T., Martinez JL., Baquero F., Canton R. y Coque TM.
Polymyxin resistance caused by mgrB inactivation is not associated with significant biological cost in Klebsiella pneumoniae
Antimicrobial Agents And Chemotherapy 59(5):2898-900.
Inactivation of the mgrB gene, encoding a negative feed-back regulator of the PhoPQ signaling system, was recently shown to be a common mutational mechanism responsible for acquired polymyxin resistance among carbapenemase-producing Klebsiella pneumoniae from clinical sources. In this work we show that mgrB mutants can easily be selected in vitro from different K. pneumoniae lineages, and that mgrB inactivation is not associated with a significant biological cost… Leer más
Cannatelli A., Santos-Lopez A., Giani T., Gonzalez-Zorn B. y Rossolini GM.
Evaluation of the risk factors contributing to the African swine fever occurrence in Sardinia, Italy
Frontiers In Microbiology 6:314.
This study assesses the relation between hypothesized risk factors and African swine fever virus (ASFV) distribution in Sardinia (Italy) after the beginning of the eradication program in 1993, using a Bayesian multivariable logistic regression mixed model. Results indicate that the probability of ASFV occurrence in Sardinia was associated to particular socio-cultural, productive and economical factors found in the region, particularly to large number of confined (i.e., closed) farms (most of them backyard), high road density, high mean altitude, large number of open fattening farms, and large number of pigs per commune. Conversely, large proportion of open farms with at least one census and large proportion of open farms per commune, were f… Leer más
Martinez-Lopez B., Perez AM., Feliziani F., Rolesu S., Mur L. y Sanchez-Vizcaino JM..
Estimation of Cultivable Bacterial Diversity in the Cloacae and Pharynx in Eurasian Griffon Vultures (Gyps fulvus)
Microbial Ecology 69:597-607.
In this work, we describe the biodiversity of cloacal and pharynx culture-based bacteria (commensal and pathogenic), in 75 Eurasian griffon vultures (Gyps fulvus) from two geographic areas. We address the question of whether the cultivable microbiota of vultures is organised into assemblages occurring by chance. In addition, we assess bacterial diversity in both anatomic regions and geographic areas. Bacterial diversity was represented by 26 Gram-negative and 20 Gram-positive genera. The most common genera were Escherichia, Enterococcus, Staphylococcus, Clostridium and Lactococcus. Escherichia coli and Enterococcus faecalis were the most common species in cloacal and pharyngeal samples. Staphylococcus and Erysipelothrix were isolated from t… Leer más
Vela AI., Casas-Diaz E., Fernandez-Garayzabal JF., Serrano E., Agustí S., Porrero MC., Sanchez V., Marco I., Lavin S. y Dominguez L..
Culturable aerobic and facultative bacteria from the gut of the polyphagic dung beetle Thorectes lusitanicus Jeckel
Insect Science 22:178-190.
Unlike other dung beetles, the Iberian geotrupid Thorectes lusitanicus exhibits polyphagous behavior; for example, it is able to eat acorns, fungi, fruits, and carrion in addition to the dung of different mammals. This adaptation to digest a wider diet has physiological and developmental advantages and requires key changes in the composition and diversity of the beetle`s gut microbiota. In this study, we isolated aerobic, facultative anaerobic, and aerotolerant microbiota amenable to grow in culture from the gut contents of T. lusitanicus and resolved isolate identity to the species level by sequencing 16S rRNA gene fragments. Using BLAST similarity searches and maximum likelihood phylogenetic analyses, we were able to reveal that the analy… Leer más
Hernandez N., Escudero JA., San Millan A., Gonzalez-Zorn B., Lobo JM., Verdu JR. y Suarez M.
Novel adenovirus detected in captive bottlenose dolphins (Tursiops truncatus) suffering from self-limiting gastroenteritis
BMC Veterinary Research 11(53):1-7.
Adenoviruses are common pathogens in vertebrates, including humans. In marine mammals, adenovirus has been associated with fatal hepatitis in sea lions. However, only in rare cases have adenoviruses been detected in cetaceans, where no clear correlation was found between presence of the virus and disease status.
A novel adenovirus was identified in four captive bottlenose dolphins with self-limiting gastroenteritis. Viral detection and identification were achieved by: PCR-amplification from fecal samples; sequencing of partial adenovirus polymerase (pol) and hexon genes; producing the virus in HeLa cells, with PCR and immunofluorescence detection, and with sequencing of the amplified pol and hexon gene fragments. A causative role of … Leer más
Rubio-Guerri C., Garcia-Parraga D., Nieto-Pelegrin E., Melero M., Alvaro T., Valls M., Crespo JL. y Sanchez-Vizcaino JM..
Method comparison for enhanced recovery, isolation and qualitative detection of C. jejuni and C. coli from wastewater effluent samples
International Journal Of Environmental Research And Public Health 12(3): 2749-2764.
Seeking a sensitive protocol, culture-dependent methods were compared to detect thermophilic Campylobacter species in untreated urban effluents. We evaluated various combinations of selective media, with and without an enrichment steps, as well as an extra filtration step. Culture-independent real-time quantitative PCR was also included and all detected isolates underwent antimicrobial susceptibility testing. All tested water samples contained Campylobacter DNA, but only 64% were positive after culture. Although enrichment using Preston broth resulted in better recovery of potentially stressed Campylobacter than Bolton or Campyfood broth (CFB), there was no significant increase in efficiency compared to direct plating. The type of selective… Leer más
Ugarte-Ruiz M., Florez-Cuadrado D., Wassenaar TM., Porrero MC. y Dominguez L..
Impact of the use of β-lactam antimicrobials on the emergence of Escherichia coli isolates resistant to cephalosporins under standard pig-rearing conditions
Applied And Environmental Microbiology 81(5):1782-7.
The aim of this study was to evaluate if the treatments with ceftiofur and amoxicillin are risk factors for the emergence of cephalosporin resistant (CR) E. coli in a pig farm during the rearing period. One hundred 7-day-old piglets were divided into two groups, a control (n = 50) group and a group parenterally treated with ceftiofur (n = 50). During the fattening period, both groups were subdivided in two. A second treatment with amoxicillin was administered in feed to two of the four groups, as follows: group 1 (untreated, n = 20), group 2 (treated with amoxicillin, n = 26), group 3 (treated with ceftiofur, n = 20), and group 4 (treated with ceftiofur and amoxicillin, n = 26). During treatment with ceftiofur, fecal samples were collected … Leer más
Cameron-Veas K., Sola-Gines M., Moreno MA., Fraile L. y Migura-Garcia L.
Freedom from equine infectious anaemia virus infection in Spanish Purebred horses
Veterinary Record Open 2(1):e000074.
Introduction: No cases of equine infectious anaemia (EIA) have been reported in Spain since 1983. Factors that could increase the risk of reintroducing equine infectious anaemia virus (EIAV) into Spain include the recent occurrence of the disease in Europe and the absence of compulsory serological testing before importation into Spain.
Aims and objectives: Given the importance of the Spanish Purebred (SP) horse breeding industry in Spain, the aim of this cross-sectional study was to provide evidence of freedom from EIAV in SP stud farms in Central Spain.
Materials and methods: Serum samples from 555 SP horses, collected between September 2011 and November 2013, were tested using a commercially available EIAV ELISA with a publi… Leer más
Cruz F., Fores P., Ireland J., Moreno MA. y Newton R.
Multiple sampling and discriminatory fingerprinting reveals clonally complex and compartmentalized infections by M. bovis in cattle
Veterinary Microbiology 175(1):99-104.
The combination of new genotyping tools and a more exhaustive sampling policy in the analysis of infection by Mycobacterium tuberculosis has shown that infection by this pathogen is more complex than initially expected. Mixed infections, coexistence of clonal variants from a parental strain, and compartmentalized infections are all different modalities of this clonal complexity. Until recently, genotyping of Mycobacterium bovis in animal populations was based on spoligotyping and analysis of a single isolate per infection; therefore, clonal complexity is probably underdetected. We used multiple sampling combined with highly discriminatory MIRU-VNTR to study compartmentalized infections by M. bovis in a low-tuberculosis prevalence setting. W… Leer más
Navarro Y., Romero B., Copano MF., Bouza E., Dominguez L., de Juan L. y Garcia de Viedma D.
Histopathological findings in cetaceans stranded on the spanish mediterranean coast between 2011 and 2014
Journal Of Comparative Pathology 152(1):89.
Introduction: A histopathological study was carried out on 11 cetaceans stranded on the southeastern Spanish Mediterranean coast between 2011 and 2014. The purpose of this study was to contribute to the surveillance of the health status of the free-ranging cetacean population.
Materials and Methods: Formalin-fixed tissue samples were submitted for examination to the Pathology Service of the Complutense University Veterinary Hospital from eight striped dolphins (Stenella coeruleoalba), two Risso’s dolphins (Grampus griseus) and one Cuvier’s beaked whale (Ziphius cavirostris).
Results: Mortality was due to trauma in 46% of the animals, infectious disease in 36% and was of unknown cause in 18% of the cases. The mo… Leer más
Jimenez MA., Rubio-Guerri C., Melero M., Crespo JL., Gallardo F., Garcia D. y Sanchez-Vizcaino JM..
An Update on the Epidemiology and Pathology of African Swine Fever
Journal Of Comparative Pathology 152(1):9-21.
African swine fever (ASF) is one of the most important infectious diseases of swine and has major negative consequences for affected countries. ASF is present in many sub-Saharan countries, Sardinia and several countries of eastern and central Europe, where its continuous spread has the swine industry on heightened alert. ASF is a complex disease for which no vaccine or treatment is available, so its control is based on early detection and rapid control of spread. For a robust and reliable early detection programme it is essential to be able to recognize the clinical signs and pathological changes of ASF, keeping in mind that in most cases the first introductions don`t show high mortality nor characteristic clinical signs or lesions, but fe… Leer más
Sanchez-Vizcaino JM., Mur L., Gomez-Villamandos JC. y Carrasco L.
Polymorphism of genes encoding PmrAB in colistin-resistant strains of Escherichia coli and Salmonella enterica isolated from poultry and swine
The Journal Of Antimicrobial Chemotherapy 70(1):71-4.
Objectives: To detect the occurrence of low susceptibility to colistin (polymyxin E), a last-resort antimicrobial, among enterobacteria isolated from samples of animal origin (poultry and swine) and to find out the molecular basis of colistin resistance. Methods: Salmonella enterica and Escherichia coli were isolated from eggs and swine samples. Bacterial strains were screened for colistin resistance by using MIC determinations interpreted according to EUCAST recommendations. pmrAB genes were amplified by PCR from bacterial isolates and their sequences were characterized.
Results: Nine colistin-resistant strains were detected in a collection of 739 enterobacteria (S. enterica and E. coli) isolated from animal samples taken in differ… Leer más
Quesada A., Porrero MC., Tellez S., Palomo G., Garcia M. y Dominguez L..
