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Detection of virulence-associated genes characteristic of intestinal Escherichia coli pathotypes, including the Enterohemorrhagic / Enteroaggregative O104:H4 in bovines from Germany and Spain

Investigación publicada en Microbiology and Immunology

1 de agosto de 2015

Cattle are reservoirs of Enterohemorrhagic Escherichia coli but their role in the epidemiology of other pathogenic E. coli remains undefined. We describe a new set of quantitative real-time PCR assays for the direct detection and quantification of nine virulence associated genes (VAGs) characteristic of the most important human E. coli pathotypes and four serotype-related genes (wzxO104 , fliCH4 , rbfO157 , fliCH7 ) that can be used as a surveillance tool for detection of pathogenic strains. A total of 970 cattle fecal samples were collected in slaughterhouses from Germany and Spain and 134 pooled samples and analyzed with this tool. Results showed that stx1, eae, and invA were more prevalent in Spanish samples while bfpA, stx2, ehxA, elt, est, and the rbfO157 /fliCH7 combination were observed in similar proportions in both countries. Genes characteristic of the hybrid O104:H4 strain of the 2011 German outbreak (stx2/aggR/wzxO104 /fliCH4 ) were simultaneously detected in six fecal pools from one German abattoir, near the outbreak epicenter. Although no isolate harboring the full stx2/aggR/wzxO104 /fliCH4 combination was cultured, sequencing of the aggR positive PCR products revealed 100% homology to the aggR from the outbreak strain. Concomitant detection of VAGs from a novel human pathogenic E. coli strain in cattle samples by this direct approach implies that the E. coli gene pool in these animals can be implicated in de novo formation of such highly-virulent strains. The application of this set of qPCRs in surveillance studies can be an efficient early-warning tool for the emergence of zoonotic E. coli in livestock




Cabal A., Geue L., Gomez S., Barth S., Barcena C., Hamm K., Porrero MC., Valverde A., Canton R., Menge C., Gortazar C., Dominguez L. y Alvarez J..




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Detection of virulence-associated genes characteristic of intestinal Escherichia coli pathotypes, including the Enterohemorrhagic / Enteroaggregative O104:H4 in bovines from Germany and Spain

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Detection of virulence-associated genes characteristic of intestinal Escherichia coli pathotypes, including the Enterohemorrhagic / Enteroaggregative O104:H4 in bovines from Germany and Spain


Participantes:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Friedrich Loeffler Institut-Bundesforschungsinstitut fuer TiergesundheitInstitute of Molecular Pathogenesis. Friedrich Loeffler Institut-Bundesforschungsinstitut fuer Tiergesundheit (FLI).

Comunidad de MadridHospital Universitario Ramón y Cajal. Consejería de Sanidad. Comunidad de Madrid.

Consejo Superior de Investigaciones CientíficasUnidad asociada de Resistencia a Antibióticos y Virulencia Bacteriana. Consejo Superior de Investigaciones Científicas (CSIC).

University of MinnesotaDepartment of Veterinary Population Medicine. College of Veterinary Medicine. University of Minnesota (UMM).

Comunidad de MadridInstituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Universidad Complutense (UCM). Universidad Autónoma de Madrid (UAM). Universidad de Alcalá (UAH). Salud Madrid. Comunidad de Madrid.







Microbiology and Immunology
FACTOR YEAR Q
1.428 2015

NLMID: 7703966

PMID: 26085084

ISSN: 1348-0421



TÍTULO: Detection of virulence-associated genes characteristic of intestinal Escherichia coli pathotypes, including the Enterohemorrhagic / Enteroaggregative O104:H4 in bovines from Germany and Spain


REVISTA: Microbiol. Immunol.


NUMERACIÓN: 59(8):433-42


AÑO: 2015


EDITORIAL: Wiley-Blackwell Publishing, inc


AUTORES: Cabal A., Geue L., Gomez S., Barth S., Barcena C., Hamm K., Porrero MC., Valverde A., Canton R., Menge C., Gortazar C., Dominguez L. and Alvarez J..


PARTICIPANTES VISAVET


3rd
Susana Gómez Barrero
5th
Carmen Bárcena Asensio
12th
Lucas Domínguez Rodríguez
Last
Julio Álvarez Sánchez

DOI: https://doi.org/10.1111/1348-0421.12275


CITA ESTA PUBLICACIÓN:

Cabal A., Geue L., Gomez S., Barth S., Barcena C., Hamm K., Porrero MC., Valverde A., Canton R., Menge C., Gortazar C., Dominguez L. y Alvarez J. Detection of virulence-associated genes characteristic of intestinal Escherichia coli pathotypes, including the Enterohemorrhagic / Enteroaggregative O104:H4 in bovines from Germany and Spain. Microbiology and Immunology. 59(8):433-42. 2015. (A). ISSN: 1348-0421. DOI: 10.1111/1348-0421.12275


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