Inicio \ Investigación \ Publicaciones científicas \ 2018

Publicaciones científicas SCI 2018

4 de diciembre de 2018

Antimicrobial Prescriptions for Dogs in the Capital of Spain

Frontiers In Veterinary Science 5:309.

     Objective: To characterize antimicrobial prescription patterns for dogs in veterinary practices in Spain using the city of Madrid as a model.
Design: Retrospective survey.
Settings: Dogs attending veterinary practices in the city of Madrid in 2017 were enrolled.
Subjects: Three hundred dogs from 30 veterinary practices randomly selected from a set of 388 practices grouped by zip code. The inclusion criterion for dogs was treatment with antibiotics within a few days of the data collection day.
Results: For the 300 dogs enrolled, 374 treatments with antimicrobials were recorded, 62.8% (235/374) were veterinary medicinal products and 37.2% (139/374) human medicinal products. The main route of administration was oral… Leer más

Gomez-Poveda B. y Moreno MA.

28 de noviembre de 2018

Spatio-temporal epidemiology of anthrax in Hippopotamus amphibious in Queen Elizabeth Protected Area, Uganda

PLoS ONE 13(11):e0206922.

Anthrax is a zoonotic disease primarily of herbivores, caused by Bacillus anthracis, a bacterium with diverse geographical and global distribution. Globally, livestock outbreaks have declined but in Africa significant outbreaks continue to occur with most countries still categorized as enzootic, hyper endemic or sporadic. Uganda experiences sporadic human and livestock cases. Severe large-scale outbreaks occur periodically in hippos (Hippopotamus amphibious) at Queen Elizabeth Protected Area, where in 2004/2005 and 2010 anthrax killed 437 hippos. Ecological drivers of these outbreaks and potential of hippos to maintain anthrax in the ecosystem remain unknown. This study aimed to describe spatio-temporal patterns of anthrax… Leer más

Dicriu M., Rwego IB., Asiimwe B., Travis DA., Alvarez J., VanderWaal K. y Pelican K.

1 de noviembre de 2018

Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles

Journal Of Veterinary Diagnostic Investigation 30(6):813-820.

     Staphylococcus aureus encompasses 2 subspecies ( aureus and anaerobius) with significant differences in their epidemiology and pathogenicity. We evaluated the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the rapid identification of both subspecies using a panel of 52 S. aureus isolates (30 subsp. anaerobius and 22 subsp. aureus) recovered from different origins, countries, and years. The on-board library identification system correctly identified 42 of 52 (81%) S. aureus isolates at the species level with score values >2.0. Limited performance was observed for differentiation of S. aureus subspecies (particularly subsp. anaerobius). Visual inspection of MALDI-TOF MS profiles … Leer más

Perez-Sancho M., Vela AI., Horcajo P., Ugarte-Ruiz M., Dominguez L., Fernandez-Garayzabal JF. y de la Fuente R.

1 de noviembre de 2018

MultiOrgan Disease and Death Associated With Streptococcus equi spp. zooepidemicus in a 2-Month-Old Foal

Journal Of Equine Veterinary Science 70:112-116.

     A 2-month-old Andalusian colt in central Spain presented dyspnea, diarrhea, and depression. Despite the initial treatment for presumptive pneumonia caused by Rhodococcus equi, after 1 hour, the colt showed signs of shock and died. The necropsy revealed multiple abscesses in the lung, as well as enlarged mesenteric lymph nodes, and hemorrhagic peritonitis. Samples were taken from trachea, lung parenchyma, small intestine, and colon. All samples yielded a pure culture of Streptococcus equi spp. zooepidemicus, whereas R. equi was excluded by using a specific qPCR performed on all samples. Isolates from all samples were characterized by pulse-field gel electrophoresis and the representative strain was identified by MALDI-TOF and qPCR. Its genom… Leer más

Dominguez-Gimbernat M., Florez-Cuadrado D., Steward K., Waller A., Porrero MC., Vela AI., Perez-Sancho M., de Juan L. y Cruz F..

1 de noviembre de 2018

Epidemiological surveillance of Mycobacterium tuberculosis complex in extensively raised pigs in the south of Spain

Preventive Veterinary Medicine 159:87-91.

     The role of domestic pigs in the epidemiology of Mycobacterium tuberculosis complex (MTC) is considered to be limited due to the characteristics of intensive production systems. However, in southwestern Spain, Iberian pigs are usually raised under extensive management systems, sharing their habitat with other domestic and wild species, some of which may act as reservoirs of MTC. Our objective was to determine the seroprevalence, risk factors, spatial distribution and spoligotypes of MTC circulating in extensively farmed pigs in Andalusia (southern Spain), a region with a high prevalence of tuberculosis in both cattle and wild boar populations. Serum samples from 3622 extensively-raised Iberian pigs from 129 randomly selected farms were test… Leer más

Cano-Terriza D., Risalde MA., Rodriguez-Hernandez P., Napp S., Fernandez-Morente M., Moreno I., Bezos J., Fernandez-Molera V., Saez-Llorente JL. y Garcia-Bocanegra I.

1 de noviembre de 2018

Use of processing fluids and serum samples to characterize porcine reproductive and respiratory syndrome virus dynamics in 3 day-old pigs

Veterinary Microbiology 225:149-156.

     Collection of serum samples of pigs at weaning to monitor for porcine reproductive and respiratory syndrome virus (PRRSV) has become a common practice to determine PRRSV herd infection status. Diagnostic sensitivity of this practice is low in herds undergoing PRRSV elimination once prevalence of infection is near zero. Thus, the goal of this study was to characterize the dynamics of PRRSV infection in 3 day-old pigs overtime using serum and serosanguineous fluids obtained as part of castration and tail docking practices (processing fluids (PF)). Secondary goal was to estimate sensitivity and specificity of PF in the 3 day old population. A 6000 breed-to-wean sow herd was monitored every three weeks for 23 weeks after a PRRSV outbreak by col… Leer más

Vilalta C., Sanhueza J., Alvarez J., Murray D., Torremorell M., Corzo C. y Morrison R.

1 de noviembre de 2018

Modeling cost-effectiveness of risk-based bovine tuberculosis surveillance in Minnesota

Preventive Veterinary Medicine 159:1-11.

     In the United States, slaughter surveillance combined with other measures has effectively maintained a very low prevalence of bovine tuberculosis (bTB). However, bTB continues to be sporadically detected, causing substantial economic burden to the government and cattle producers. To detect the infection earlier and reduce sudden economic losses, additional risk-based surveillance of live animals might be more cost-effective than slaughter surveillance alone to detect and prevent bTB infection. The objective of this study was to evaluate alternative risk-based surveillance strategies targeting high-risk herds to complement slaughter surveillance in a region with very low bTB prevalence. We developed an integrated within- and between-herd bTB… Leer más

Zoe Kao SY., VanderWaal K., Enns EA., Craft ME., Alvarez J., Picasso C. y Wells SJ.

24 de octubre de 2018

Current concepts and contents of Veterinary Public Health and Veterinary Health Polic

Revista Española De Salud Pública 92:e201810077.

     Human and veterinary medicine obviously share most concepts and contents. However, both academic and scientific issues and the daily practice of many health disciplines can differ largely between human and veterinary medicine. Such differences can easily go unnoticed among other healthcare collectives or the general public. The aim of the present work was to provide an update, as well as some definitions of what we understand under the terms Veterinary Public Health and Veterinary Health Policy… Leer más

Briones V., Bezos J. y Alvarez J..

23 de octubre de 2018

Ant(6)-I genes encoding aminoglycoside O-nucleotidyltransferases are widely spread among streptomycin resistant strains of Campylobacter jejuni and Campylobacter coli

Frontiers In Microbiology 9:2515.

     Thermotolerant Campylobacter species C. jeuni and C. coli are actually recognized as the major bacterial agent responsible for food-transmitted gastroenteritis. The most effective antimicrobials against Campylobacter are macrolides and some, but not all aminoglycosides. Among these, susceptibility to streptomycin is reduced by mutations in the ribosomal RPSL protein or by expression of ANT(6)-I aminoglycoside O-nucleotidyltransferases. The presence of streptomycin resistance genes was evaluated among streptomycin-resistant Campylobacter isolated from humans and animals by using PCR with degenerated primers devised to distinguish ant(6)-Ia, ant(6)-Ib and other ant-like genes. Genes encoding ANT(6)-I enzymes were found in all possible combina… Leer más

Hormeno L., Ugarte-Ruiz M., Palomo G., Borge C., Vadillo S., Piriz S., Dominguez L., Campos MJ. y Quesada A.

5 de octubre de 2018

Pathologic findings and causes of death of stranded cetaceans in the Canary Islands (2006-2012)

PLoS ONE 13(10): e0204444.

     This study describes the pathologic findings and most probable causes of death (CD) of 224 cetaceans stranded along the coastline of the Canary Islands (Spain) over a 7-year period, 2006-2012. Most probable CD, grouped as pathologic categories (PCs), was identified in 208/224 (92.8%) examined animals. Within natural PCs, those associated with good nutritional status represented 70/208 (33.6%), whereas, those associated with significant loss of nutritional status represented 49/208 (23.5%). Fatal intra- and interspecific traumatic interactions were 37/208 (17.8%). Vessel collisions included 24/208 (11.5%). Neonatal/perinatal pathology involved 13/208 (6.2%). Fatal interaction with fishing activities comprised 10/208 (4.8%). Within anthropoge… Leer más

Diaz-Delgado J., Fernandez A., Sierra E., Sacchini S., Andrada M., Vela AI., Quesada-Canales O., Paz Y., Zucca D., Groch K. y Arbelo M.

1 de octubre de 2018

Response of goats to intramuscular vaccination with heat-killed Mycobacterium bovis and natural challenge

Comparative Immunology, Microbiology And Infectious Diseases 60:28-34.

     Caprine TB causes chronic disease with severe economic and health consequences. We assessed the effect of intramuscularly administered heat-inactivated Mycobacterium bovis (M. bovis) on 20 kid goats (10 vaccinated, 10 controls), naturally exposed to M. caprae through close contact with infected goats. At necropsy, visible TB-compatible lesions were recorded in all animals with the exception of 1 control and 2 vaccinated goats. The median of the total lesion score was 9 (IQR 3-13.5) and 5 (IQR 3-6.75) in control and vaccinated goats, respectively (median lesion reduction 44.4%, p =  0.224). The lung lesions of the vaccinated goats were restricted to the caudal lobes, while 6 controls had additional lung lobes affected (p =&… Leer más

Roy A., Risalde MA., Bezos J., Casal C., Romero B., Sevilla I., Diez-Guerrier A., Rodriguez-Bertos A., Dominguez M., Garrido J., Gortazar C. y Dominguez L..

1 de octubre de 2018

Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level

Journal Of Fish Diseases 41(10):1485-1493.

     The aim of this study was to evaluate the usefulness of the MALDI-TOF MS to identify 151 isolates of Aeromonas obtained mostly from diseased fish. MALDI-TOF MS correctly identified all isolates to the genus level but important differences in the percentage of isolates correctly identified depending on the species were observed. Considering exclusively the first identification option, Aeromonas bestiarum, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas veronii and Aeromonas sobria were the best identified with results >95%. However, considering the first and second identification options, the only species that showed values >90% was A. hydrophila. Overall, when the database was supplemented with 14 new spectra, the number of accurate … Leer más

Perez-Sancho M., Cerda I., Fernandez-Bravo A., Dominguez L., Figueras MJ., Fernandez-Garayzabal JF. y Vela AI..

1 de octubre de 2018

Management of hunting waste as control measure for tuberculosis in wild ungulates in south-central Spain

Transboundary And Emerging Diseases 65(5):1190-1196.

     In recent decades, habitat change and the intensive management of wild ungulates for hunting have led to an increase in their populations in south-central Spain. This implies a higher generation of hunting waste, which can favour the transmission of infectious diseases, including tuberculosis (TB). The aim of this study was to assess the usefulness of the proper disposal of hunting waste as TB control measure in wild boar (Sus scrofa) and red deer (Cervus elaphus) during the 2008/2009 to 2016/2017 hunting seasons. Blood samples from 664 wild boar and 934 red deer were obtained in 14 game estates in two provinces in Andalusia (Area 1), where the disposal of hunting waste was implemented since the 2012/2013 hunting season. Besides, six game e… Leer más

Cano-Terriza D., Risalde MA., Jiménez-Ruiz S., Vicente J., Isla J., Paniagua J., Moreno I., Gortazar C., Infantes-Lorenzo JA. y Garcia-Bocanegra I.

29 de septiembre de 2018

Carriage of antibiotic-resistant bacteria in urban versus rural wild boars

European Journal Of Wildlife Research 64: 60.

     The Western European population of wild boar (Sus scrofa) has increased its distribution over the past several decades, and some populations have colonized areas strongly influenced by human activity. Wild boars are known carriers of antibiotic-resistant bacteria acquired from the environment, and urban populations of wild boars may be more exposed than their rural counterparts. In this work, we compared the frequency of antibiotic resistance in indicator bacteria (Escherichia coli, Enterococcus faecalis, Enterococcus faecium) isolated from urban wild boars with that from rural wild boars in NE Spain. We further assessed whether bacterial isolates from the urban wild boars had a higher probability of showing antibiotic resistance when their… Leer más

Navarro-Gonzalez N., Castillo Contreras R., Casas Diaz E., Morellet N., Porrero MC., Molina-Vacas G., Torres RT., Fonseca C., Mentaberre G., Dominguez L., Lavin S. y Serrano E.

27 de septiembre de 2018

Evaluation of the performance of the IDvet IFN-gamma test for diagnosis of bovine tuberculosis in Spain

Frontiers In Veterinary Science 229(5):1-9.

     In Spain, the national bovine tuberculosis (bTB) eradication program is based on yearly skin testing of every ≥6 weeks old animal using the single or comparative tuberculin test and parallel use of the interferon-gamma (IFN-γ) assay as an ancillary diagnostic test in infected herds. There are several versions of the latter. Recently, a new commercial IDvet IFN-γ assay has been authorized for use in the program, but there is limited scientific evidence about its performance in different epidemiological settings. Therefore, two studies to evaluate the performance of the IDvet assay were conducted. In study 1, a concordance analysis between the new IDvet and the Bovigam IFN-γ assay in use in Spain for over 10 years was con… Leer más

de la Cruz ML., Branscum AJ., Nacar J., Pages E., Pozo P., Perez AM., Grau A., Saez-Llorente JL., de Juan L., Diaz R., Minguez O. y Alvarez J..

1 de septiembre de 2018

Educating in antimicrobial resistance awareness: adaptation of the Small World Initiative program to service-learning

FEMS Microbiology Letters 365(17):1-22.

     The Small World Initiative (SWI) is a consolidated and successful education program rooted in the USA that tackles the antibiotic crisis by a crowdsourcing strategy. Based on active learning, it challenges young students to discover novel bioactive-producing microorganisms form environmental soil samples. Besides its pedagogical efficiency to impart Microbiology contents in academic curricula, SWI promotes vocations on research and development in Experimental Sciences and, at the same time, disseminates the antibiotic awareness guidelines of the World Health Organization (WHO). We have adapted the SWI program to the Spanish academic environment by a pioneering hierarchic strategy based on service-learning that involves two education levels … Leer más

Valderrama MJ., Gonzalez-Zorn B., Calvo de Pablo P., Diez Orejas R., Fernandez Acero T., Gil Serna J., de Juan L., Martin H., Molina M., Navarro-Garcia F., Patino B., Pla J., Prieto D., Rodriguez C., Roman E., Sanz Santamaría AB., de Siloniz MI., Suarez M., Vazquez C. y Cid VJ.

1 de septiembre de 2018

Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguays University Hospital Identifies the First rmtC Gene in the Species

Microbial Drug Resistance (Larchmont, N.Y.) 24(7):1012-1019.

     Carbapenem-resistant Acinetobacter baumannii (CRAB) infections are an increasing concern in intensive care units (ICUs) worldwide. The combination of carbapenemases and 16S rRNA-methyltransferases (16S-RMTases) further reduces the therapeutic options. OXA-carbapenemase/A. baumannii clone tandems in Latin America have already been described; however, no information exists in this region regarding the occurrence of 16S-RMTases in this microorganism. In addition, the epidemiology of A. baumannii in ICUs and its associated resistance profiles are poorly understood. Our objectives were as follows: to study the clonal relationship and antibiotic resistance profiles of clinical and digestive colonizing A. baumannii isolates in an ICU, to character… Leer más

Bado I., Papa-Ezdra R., Delgado-Blas JF., Gaudio M., Gutierrez C., Cordeiro NF., Garcia-Fulgueiras V., Pirez LA., Seija V., Medina JC., Rieppi G., Gonzalez-Zorn B. y Vignoli R.

1 de septiembre de 2018

Novel and highly sensitive sybr® green real-time pcr for poxvirus detection in odontocete cetaceans

Journal Of Virological Methods 259:45-49.

     Poxviruses are emerging pathogens in cetaceans, temporarily named `Cetaceanpoxvirus` (CePV, family Poxviridae), classified into two main lineages: CePV-1 in odontocetes and CePV-2 in mysticetes. Only a few studies performed the molecular detection of CePVs, based on DNA-polymerase gene and/or DNA-topoisomerase I gene amplification. Herein we describe a new real-time PCR assay based on SYBR® Green and a new primer set to detect a 150 bp fragment of CePV DNA-polymerase gene, also effective for conventional PCR detection. The novel real-time PCR was able to detect 5 up to 5 × 106 copies per reaction of a cloned positive control. Both novel PCR methods were 1000 to 100,000-fold more sensitive than those previously described in the l… Leer más

Sacristan C., Luiz Catao-Dias J., Ewbank AC., Machado EF., Neves E., Santos-Neto EB., Avezedo A., Laison-Brito J., De Castillo PV., Daura-Jorge FG., Simoes-Lopes PC., Carballo M., Garcia-Parraga D., Sanchez-Vizcaino JM. y Esperon F.

24 de agosto de 2018

Genetic heterogeneity of dolphin morbilliviruses detected in the Spanish Mediterranean in inter-epizootic period

BMC Veterinary Research 14(1):248.

In the last 20 years, Cetacean Morbillivirus (CeMV) has been responsible for many die-offs in marine mammals worldwide, as clearly exemplified by the three dolphin morbillivirus (DMV) epizootics of 1990-1992, 2006-2008 and 2011 that affected Mediterranean striped dolphins (Stenella coeruleoalba). Systemic infection caused by DMV in the Mediterranean has been reported only during these outbreaks.

We report the infection of five striped dolphins (Stenella coeruleoalba) stranded on the Spanish Mediterranean coast of Valencia after the last DMV outbreak that ended in 2011. Animal 1 stranded in late 2011 and Animal 2 in 2012. Systemic infection affecting all tissues was found based on histopathol… Leer más

Rubio-Guerri C., Jimenez MA., Melero M., Diaz-Delgado J., Sierra E., Arbelo M., Belliere EN., Crespo JL., Garcia-Parraga D., Esperon F. y Sanchez-Vizcaino JM..

13 de agosto de 2018

Development and Evaluation of a Serological Assay for the Diagnosis of Tuberculosis in Alpacas and Llamas

Frontiers In Veterinary Science 5(189):1-7.

     South American camelids are susceptible to tuberculosis, caused mainly by Mycobacterium bovis and M. microti. Despite the tuberculin skin test being the official test for tuberculosis, it has a very low sensitivity in these species (14–20%). Serological tests present the advantages of being rapid, easy to perform and facilitate analysis of large numbers of samples in a short period of time. Novel antigen discovery and evaluation would provide enhanced detection of specific antibodies against members of M. tuberculosis complex. Here, we describe the development and evaluation of an ELISA-type immunoassays to use in the diagnosis of tuberculosis in llamas and alpacas based on P22, a multiprotein complex obtained by affinity chromatography fro… Leer más

Infantes-Lorenzo JA., Whiyehead CE., Moreno I., Bezos J., Roy A., Dominguez L., Dominguez M. y Salguero FJ.

3 de agosto de 2018

Abundance and diversity of the faecal resistome in slaughter pigs and broilers in nine European countries

Nature Microbiology 3(8):898-908.

     EFFORT Group
Haitske Graveland2 , Alieda van Essen4, Bruno Gonzalez-Zorn8, Gabriel Moyano8, Pascal Sanders9, Claire Chauvin9, Julie David9, Antonio Battisti10, Andrea Caprioli10, Jeroen Dewulf11, Thomas Blaha12, Katharina Wadepohl12, Maximiliane Brandt12, Dariusz Wasyl13, Magdalena Skarzyńska13, Magdalena Zajac13, Hristo Daskalov14, Helmut W. Saatkamp4 and Katharina D. C. Stärk15
Antimicrobial resistance (AMR) in bacteria and associated human morbidity and mortality is increasing. The use of antimicrobials in livestock selects for AMR that can subsequently be transferred to humans. This flow of AMR between reservoirs demands
surveillance in livestock and in humans. We quantified and characterized the acquired resis… Leer más

Munk P., Knudsen BE., Lukjacenko O., Ribeiro-Duarte AS., Van Gompel L., Luiken REC., Smit LAM., Schmitt H., Dorado Garcia A., Borup-Hansen R., Nordahl-Petersen T., Bossers A., Ruppe E., EFFORT Group., Lund O., Hald T., Pamp SJ., Vigre H., Heederik D., Wagenaar J., Mevius D. y Aarestrup FM.

1 de agosto de 2018

Exploring the oxidative, antimicrobial and genomic properties of Campylobacter jejuni strains isolated from poultry

Research In Veterinary Science 119:170-175.

     Campylobacter jejuni is the leading cause of food-borne bacterial enteritis in humans, with contaminated poultry products considered the main source of infection. To survive the food chain, C. jejuni utilizes multiple defense mechanisms that counter oxidative and aerobic stresses. In this study, we phenotypically characterised 63 C. jejuni strains with oxidative stress survival and antimicrobial susceptibility testing to investigate correlations between these two phenotypes against the source of the strains and the presence of the MarR regulators RrpA and RrpB which have a role in regulating the response to oxidative and aerobic stress. C. jejuni strains isolated from meat and neck skin displayed the highest resistance to oxidative stress. … Leer más

Ugarte-Ruiz M., Dominguez L., Corcionivoschi N., Brendan WW., Dorrell N. y Gundogdu O.

1 de agosto de 2018

Evaluation of the use of a needle-free injection syringe as a cause of non-specific reactions in the intradermal tuberculin test used for the diagnosis of bovine tuberculosis

Research In Veterinary Science 119:56-60.

     The objective of the study was to elucidate whether the use of the needle-free Dermojet syringe, which is based on a high pressure inoculation and is used to inject tuberculin in cattle in several countries, may, in itself, cause skin reactions that can be interpreted as positive reactions to the intradermal tests that are not, in fact, related to the real infection status of the animals.
Forty-four cattle from an officially tuberculosis-free (OTF) herd were selected, and four single intradermal tuberculin (SIT) tests were performed on each animal, two on each side of the neck. Three different Dermojet (D1, D2 and D3) and one McLintock (M4) syringes were used to carry out sterile phosphate buffer saline (PBS) with 10% of glycerol and… Leer más

Diez-Guerrier A., Roy A., de la Cruz ML., Saez-Llorente JL., Sanz C., Boschiroli ML., Romero B., de Juan L., Dominguez L. y Bezos J..

1 de agosto de 2018

Temporal analysis of the interference caused by paratuberculosis vaccination on the tuberculosis diagnostic tests in goats

Preventive Veterinary Medicine 156:68-75.

     Vaccination against paratuberculosis (PTB) in goats is a cost-effective control strategy, and is also effective as regards preventing the onset of clinical cases. However, it causes interference in the diagnostic tests used in the control of tuberculosis (TB). A group of 99 goats from a herd with no history of TB or PTB infection was vaccinated against PTB at seven months of age. They then underwent consecutive intradermal tests [single (SIT) and comparative (CIT) intradermal tuberculin tests), interferon-gamma release assays (IGRA) and two serological tests (p22_CE and DR-ELISA) every three months, until the interference disappeared. When using the SIT test, a variable number of positive reactors were observed at 3 months (T3; 32.3%, 95% C… Leer más

Roy A., Infantes-Lorenzo JA., Blazquez JC., Venteo A., Mayoral-Alegre F., Dominguez M., Moreno I., Romero B., de Juan L., Grau A., Dominguez L. y Bezos J..

1 de agosto de 2018

Genetic dissection of the BRCA2 promoter and transcriptional impact of DNA variants

Breast Cancer Research And Treatment 171(1):53-63.

Promoter mutations may affect transcription and can be associated with human diseases. However, the promoters of the breast cancer (BC) genes are not regularly screened. Our goal was to investigate the BRCA2 promoter in order to study a possible correlation between impaired transcription and disease.
The proximal and core promoter of the BRCA2 gene was sequenced in 95 high-risk BC patients. A BRCA2-promoter insert [− 938 to + 312 from the transcription start site (TSS)] was generated and cloned into the firefly luciferase vector pGL4.10. Promoter variants and deletions were introduced by site-directed mutagenesis and quantified by Dual-Luciferase assays and semi-quantitative RT-PCR.
Re… Leer más

Fraile-Bethencourt E., Valenzuela-Palomo A., Diez-Gomez B., Infante M., Duran M., Marcos G., Lastra E., Gomez S. y Velasco EA.

1 de agosto de 2018

Epidemiological situation of the exposure to agents causing Equine Piroplasmosis in Spanish Purebred Horses in Spain: seroprevalence and associated risk factors

Journal Of Equine Veterinary Science 67:81-86.

     Equine piroplasmosis (EP) is a tick-borne disease caused by Babesia caballi (B. caballi) and Theileria equi (T. equi) which causes severe economic losses to the equine industry, represented by the Spanish Purebred (SP) horse in Spain. The seroprevalence of EP is known in certain regions of Spain but up to date there are no data in SP horses. The aim of this study was to determine the seroprevalence of EP in the SP breeding horse population in central Spain and to evaluate risk factors associated with the occurrence of the infection. A total of 536 horse serum samples were tested for antibodies against T. equi and B. caballi by competitive inhibition ELISA. Data on possible risk factors were examined using two logistic regression models with… Leer más

Camino E., de la Cruz ML., Dominguez L., Carvajal KA., Fores P., de Juan L. y Cruz F..

27 de julio de 2018

Different lesion distribution in calves orally or intratracheally challenged with Mycobacterium bovis: implications for diagnosis

Veterinary Research 49(1):74.

     Animal tuberculosis (TB) remains a major problem in some countries despite the existence of control programmes focused mainly on cattle. In this species, aerogenous transmission is accepted as the most frequent infection route, affecting mainly the respiratory system. Under the hypothesis that the oral route could be playing a more relevant role in transmission, diagnosis and disease persistence than previously thought, this study was performed to assess the course of TB infection in cattle and its effects on diagnosis depending on the route of entry of Mycobacterium bovis. Two groups of five calves each were either endotracheally (EC) or orally (OC) challenged. Necropsies were carried out 12 weeks after challenge except for three OC calves… Leer más

Serrano M., Sevilla AI., Fuertes M., Geijo M., Risalde MA., Ruiz-Fons F., Gortazar C., Juste RA., Dominguez L., Elguezabal N. y Garrido JM.

15 de julio de 2018

Detection of Toxoplasma gondii in three common bottlenose dolphins (Tursiops truncatus); A first description from the Eastern Mediterranean Sea

Veterinary Parasitology 258:74-78.

     Toxoplasma gondii has been described in several marine mammals around the world including numerous species of cetaceans, yet infection and transmission mechanisms in the marine environment are not clearly defined. The Israel Marine Mammal Research and Assistance Center has been collating a database of all marine mammal stranding events along the country’s national coastlines since 1993. In this study, we describe the molecular detection and characterisation of T. gondii in three common bottlenose dolphins (Tursiops truncatus) including one case of coinfection with herpesvirus. The animals were found stranded on the Mediterranean coast of Israel in May and November 2013. In one of the three cases, the dolphin was found alive and admitted to … Leer más

Bigal E., Morick D., Scheinin AP., Salant H., Berkowitz A., King R., Levy Y., Melero M., Sanchez-Vizcaino JM., Goffman O., Hadar N., Roditi-Elasar M. y Tchernov D.

1 de julio de 2018

Impact of piglet oral vaccination against tuberculosis in endemic free-ranging wild boar populations

Preventive Veterinary Medicine 155:11-20.

     The Eurasian wild boar (Sus scrofa) is the main wild reservoir of the Mycobacterium tuberculosis complex in Mediterranean woodlands and a key risk factor for cattle tuberculosis (TB) breakdowns. Wild boar vaccination therefore has the potential to be a valuable tool for TB control. We tested two orally delivered vaccines, heat-inactivated Mycobacterium bovis (IV) and BCG, in four sites (two per vaccine type: one Managed and one Natural or unmanaged) during four years. TB was also monitored in 15 unvaccinated sites (spatial control), as well as in all sites from one year prior to intervention (temporal control). The rationale is that by vaccinating 2–6 month old wild boar piglets we can reduce disease at the population level during the study… Leer más

Diez-Delgado I., Sevilla IA., Romero B., Tanner E., Barasona JA., White AR., Lurz PWW., Boots M., de la Fuente J., Dominguez L., Vicente J., Garrido JM., Juste RA., Aranaz A. y Gortazar C.

1 de junio de 2018

Human Pasteurella multocida Infection with Likely Zoonotic Transmission from a Pet Dog, Spain

Emerging Infectious Diseases 24(6):1145-1146.

     We report a case of urinary tract infection caused by an unusual genotype (sequence type 211) of Pasteurella multocida associated with human infection. Molecular genetic analysis of P. multocida isolates obtained from the human patient and his pet strongly suggests a zoonotic transmission of this bacterium… Leer más

Abreu F., Rodriguez-Lucas C., Rodicio MR., Vela AI., Fernandez-Garayzabal JF., Leiva PS., Cuesta F., Cid D. y Fernandez J.

1 de junio de 2018

Ovine Mannheimia haemolytica isolates from lungs with and without pneumonic lesions belong to similar genotypes

Veterinary Microbiology 219:80-86.

     This study investigated the genetic characteristics of 121 ovine Mannheimia haemolytica isolates from lungs with (n = 75) and without pneumonic lesions (n = 46) using multilocus sequence typing (MLST), virulence-associated gene typing and pulsed-field gel electrophoresis (PFGE). Twelve STs were identified with most isolates (81%) belonged to ST16, ST28 and ST8. Analysis of the M. haemolytica MLST Database indicate a wide distribution of these genotypes in small ruminants, never reported in bovine isolates. This could suggest the adaptation of certain genetic lineages of M. haemolytica to small ruminants. e-BURST analysis grouped most STs into three clonal complexes (CC2, CC8 and CC28), consistent with a clonal popula… Leer más

Garcia-Alvarez A., Fernandez-Garayzabal JF., Chaves F., Pinto C. y Cid D.

2 de mayo de 2018

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Journal Of Visualized Experiments : JoVE (135):57386.

     Multicopy plasmids are extremely abundant in prokaryotes but their role in bacterial evolution remains poorly understood. We recently showed that the increase in gene copy number per cell provided by multicopy plasmids could accelerate the evolution of plasmid-encoded genes. In this work, we present an experimental system to test the ability of multicopy plasmids to promote gene evolution. Using simple molecular biology methods, we constructed a model system where an antibiotic resistance gene can be inserted into Escherichia coli MG1655, either in the chromosome or on a multicopy plasmid. We use an experimental evolution approach to propagate the different strains under increasing concentrations of antibiotics and we measure survival of ba… Leer más

Escudero JA., MacLean RC. y San Millan A.

1 de mayo de 2018

The use of serological tests in combination with the intradermal tuberculin test maximizes the detection of tuberculosis infected goats

Veterinary Immunology And Immunopathology 199:43-52.

     The diagnosis of tuberculosis (TB) in goats is based mainly on the single and comparative intradermal tuberculin (SIT and CIT) tests and, exceptionally, on the interferon-gamma (IFN-γ) assay, however they are not perfect in terms of sensitivity and specificity. Nevertheless, various serological assays that provide a potential cost-effective approach for the control of TB are also available or under development, and a variety of results have been reported regarding the ability of these tests to detect infected animals, particularly in the early stages of infection.
In the present study, SIT/CIT and IFN-γ tests and three different serological assays were evaluated during two consecutive herd testing events in a recently infec… Leer más

Bezos J., Roy A., Infantes-Lorenzo JA., Gonzalez I., Venteo A., Romero B., Grau A., Minguez O., Dominguez L. y de Juan L..

1 de mayo de 2018

Streptococcus penaeicida sp. nov., isolated from a diseased farmed Pacific white shrimp (Penaeus vannamei)

International Journal Of Systematic And Evolutionary Microbiology 68(5):1490-1495.

     Strain CAIM 1838T, isolated from the hepatopancreas of a cultured diseased Pacific white shrimp (Penaeusvannamei), was subjected to characterization by a polyphasic taxonomic approach. On the basis of 16S rRNA gene sequence analysis, strain CAIM 1838T was most closely related to Streptococcus bovimastitidis 99.3 % and to other species of the Pyogenes clade of Streptococcus with lower similarity values. Average nucleotide identity values and the genome-to-genome distance of strain CAIM 1838T, as compared with the type strains, confirmed the separate species status with closely related species of the genus Streptococcus and were all below the thresholds to delimit a species, 93.1 and 49.4 %, respectively. The DNA G+C content… Leer más

Morales-Covarrubias MS., del Carmen Bolan-Mejía M., Vela AI., Fernandez-Garayzabal JF. y Gomez-Gil B.

1 de mayo de 2018

Plasmids carrying antimicrobial resistance genes in Enterobacteriaceae

The Journal Of Antimicrobial Chemotherapy 73(5):1121-1137.

     Bacterial antimicrobial resistance (AMR) is constantly evolving and horizontal gene transfer through plasmids plays a major role. The identification of plasmid characteristics and their association with different bacterial hosts provides crucial knowledge that is essential to understand the contribution of plasmids to the transmission of AMR determinants. Molecular identification of plasmid and strain genotypes elicits a distinction between spread of AMR genes by plasmids and dissemination of these genes by spread of bacterial clones. For this reason several methods are used to type the plasmids, e.g. PCR-based replicon typing (PBRT) or relaxase typing. Currently, there are 28 known plasmid types in Enterobacteriaceae distinguished by PBRT.… Leer más

Rozwandowicz M., Brouwer MSM., Fischer J., Wagenaar J., Gonzalez-Zorn B., Guerra B., Mevius DJ. y Hordijk J.

1 de mayo de 2018

Gaps in African swine fever: Analysis and priorities

Transboundary And Emerging Diseases 65 Suppl 1:235-247.

     African swine fever (ASF) causes greater sanitary, social and economic impacts on swine herds than many other swine diseases. Although ASF was first described in 1921 and it has affected more than fifty countries in Africa, Europe and South America, several key issues about its pathogenesis, immune evasion and epidemiology remain uncertain. This article reviews the main characteristics of the causative virus, its molecular epidemiology, natural hosts, clinical features, epidemiology and control worldwide. It also identifies and prioritizes gaps in ASF from a horizontal point of view encompassing fields including molecular biology, epidemiology, prevention, diagnosis and vaccine development. The purpose of this review is to promote ASF resea… Leer más

Arias M., Jurado C., Gallardo C., Fernandez-Pinero J. y Sanchez-Vizcaino JM..

16 de abril de 2018

Relevant Measures to Prevent the Spread of African Swine Fever in the European Union Domestic Pig Sector

Frontiers In Veterinary Science 5:77.

     During the past decade, African swine fever (ASF) has spread from the Caucasus region to eastern European Union countries affecting domestic pig and wild boar populations. In order to avert ASF spread, mitigation measures targeting both populations have been established. However, despite these efforts, ASF has been reported in thirteen different countries (Georgia, Azerbaijan, Armenia, the Russian Federation, Ukraine, Belarus, Estonia, Latvia, Lithuania, Poland, Moldova, Czech Republic, and Romania). In the absence of an effective vaccine or treatment to ASF, introduction and spread of ASF onto domestic pig farms can only be prevented by strict compliance to control measures. This study systematically reviewed available measures to prevent … Leer más

Jurado C., Martinez-Aviles M., de la Torre A., Stukelj M., Cardoso de Carvalho-Ferreira H., Cerioli M., Sanchez-Vizcaino JM. y Bellini S.

1 de abril de 2018

Why is African swine fever still present in Sardinia?

Transboundary And Emerging Diseases 65(2):557-566.

     African swine fever (ASF) is an infectious disease of swine that has been present in Sardinia since 1978. Soon after introduction of the disease, several control and eradication programmes were established with limited success. Some researchers attributed the persistence of the disease in central and eastern areas to certain socio-economic factors, the existence of some local and traditional farming practices (i.e., unregistered free-ranging pigs known as brado animals) and the high density of wild boar in the region. In the past, scarcity of swine data in Sardinia complicated the evaluation and study of ASF on the island. More complete, accurate and reliable information on pig farms has become available as a result of the most recent eradi… Leer más

Jurado C., Fernandez-Carrion E., Mur L., Rolesu S., Laddomada A. y Sanchez-Vizcaino JM..

27 de marzo de 2018

In vivo transfer and microevolution of avian native IncA/C2blaNDM-1-carrying plasmid pRH-1238 during a broiler chicken infection study

Antimicrobial Agents And Chemotherapy 62(4):e02128-17.

     The emergence and spread of carbapenemase-producing Enterobacteriaceae (CPE) in wildlife and livestock animals poses an important safety concern for public health. With our in vivo broiler chicken infection study we investigated transfer and experimental microevolution of the blaNDM-1-carrying IncA/C2 plasmid (pRH-1238) introduced by avian native Salmonella (S.) Corvallis, without inducing antibiotic selection pressure. We evaluated dependency of the time point of inoculation on donor [S Corvallis (12-SA01738)] and a plasmid-free Salmonella spp. recipient [S Paratyphi B (dTa+), 13-SA01617] excretion by quantifying their excretion dynamics. Using S1-PFGE plasmid profiling we gained insight into the variability of native plasmid content among… Leer más

Hadziabdic S., Fischer J., Malorny B., Borowiak M., Guerra B., Kaesbohrer A., Gonzalez-Zorn B. y Szabo I.

22 de marzo de 2018

An advection-deposition-survival model to assess the risk of introduction of vector-borne diseases through the wind: Application to bluetongue outbreaks in Spain

PLoS ONE 13(3):e0194573.

     This work develops a methodology for estimating risk of wind-borne introduction of flying insects into a country, identifying areas and periods of high risk of vector-borne diseases incursion. This risk can be characterized by the role of suitable temperatures and wind currents in small insects` survival and movements, respectively. The model predicts the number density of introduced insects over space and time based on three processes: the advection due to wind currents, the deposition on the ground and the survival due to climatic conditions. Spanish livestock has suffered many bluetongue outbreaks since 2004 and numerous experts point to Culicoides transported by wind from affected areas in North Africa as a possible cause. This work imp… Leer más

Fernandez-Carrion E., Ivorra B., Ramos AM., Martinez-Lopez B., Aguilar-Vega C. y Sanchez-Vizcaino JM..

16 de marzo de 2018

PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samples Reveals Their Importance as Gene Capture Platforms

Frontiers In Microbiology 9(469):1-15.

     ColE1 plasmids are important vehicles for the spread of antibiotic resistance in the Enterobacteriaceae and Pasteurellaceae families of bacteria. Their monitoring is essential, as they harbor important resistant determinants in humans, animals and the environment. In this work, we have analyzed ColE1 replicons using bioinformatic and experimental approaches. First, we carried out a computational study examining the structure of different ColE1 plasmids deposited in databases. Bioinformatic analysis of these ColE1 replicons revealed a mosaic genetic structure consisting of a host-adapted conserved region responsible for the housekeeping functions of the plasmid, and a variable region encoding a wide variety of genes, including multiple antib… Leer más

Ares M., Bernabe-Balas C., Santos-Lopez A., Baquero MR., Prasad KN., Cid D., Martin-Espada C., San Millan A. y Gonzalez-Zorn B..

28 de febrero de 2018

Phylodynamics and evolutionary epidemiology of African swine fever p72-CVR genes in Eurasia and Africa

PLoS ONE 13(2):e0192565.

     African swine fever (ASF) is a complex infectious disease of swine that constitutes devastating impacts on animal health and the world economy. Here, we investigated the evolutionary epidemiology of ASF virus (ASFV) in Eurasia and Africa using the concatenated gene sequences of the viral protein 72 and the central variable region of isolates collected between 1960 and 2015. We used Bayesian phylodynamic models to reconstruct the evolutionary history of the virus, to identify virus population demographics and to quantify dispersal patterns between host species. Results suggest that ASFV exhibited a significantly high evolutionary rate and population growth through time since its divergence in the 18th century from East Africa, with no signs … Leer más

Alkhamis MA., Gallardo C., Jurado C., Soler A., Arias M. y Sanchez-Vizcaino JM..

1 de febrero de 2018

Understanding Q Fever Risk to Humans in Minnesota Through the Analysis of Spatiotemporal Trends

Vector Borne And Zoonotic Diseases 18(2):89-95.

     Q fever is a widely distributed, yet, neglected zoonotic disease, for which domestic ruminants are considered the main reservoirs in some countries. There are still many gaps in our knowledge of its epidemiology, and the source of sporadic cases is often not determined. In this study, we show how Q fever surveillance data in combination with information routinely collected by government agencies in Minnesota during 1997 to 2015 can be used to characterize patterns of occurrence of Q fever illnesses and detect variables potentially associated with increased human illness. Cluster analysis and Bayesian spatial regression modeling revealed the presence of areas in Southern Minnesota at higher risk of Q fever. The number of sheep flocks at the … Leer más

Alvarez J., Whitten T., Branscum AJ., Garcia-Seco T., Bender JB., Scheftel J. y Perez AM.

1 de febrero de 2018

Understanding African Swine Fever infection dynamics in Sardinia using a spatially explicit transmission model in domestic pig farms

Transboundary And Emerging Diseases 65(1):123-134.

     African swine fever virus (ASFV) has been endemic in Sardinia since 1978, resulting in severe losses for local pig producers and creating important problems for the island`s veterinary authorities. This study used a spatially explicit stochastic transmission model followed by two regression models to investigate the dynamics of ASFV spread amongst domestic pig farms, to identify geographic areas at highest risk and determine the role of different susceptible pig populations (registered domestic pigs, non-registered domestic pigs [brado] and wild boar) in ASF occurrence. We simulated transmission within and between farms using an adapted version of the previously described model known as Be-FAST. Results from the model revealed a generally l… Leer más

Mur L., Sanchez-Vizcaino JM., Fernandez-Carrion E., Jurado C., Rolesu S., Feliziani F., Laddomada A. y Martinez-Lopez B.

25 de enero de 2018

Emergence of chromosome borne colistin resistance gene mcr-1 in clinical isolates of Klebsiella pneumoniae from India

Antimicrobial Agents And Chemotherapy 62(2):e01885-17.

     Transmissible colistin resistance gene, mcr-1 has breached the one of the last line of defense for treatment of infections caused by carbapenem-resistant Enterobacteriaceae… Leer más

Singh S., Pathak A., Kumar A., Rahman M., Singh A., Gonzalez-Zorn B. y Prasad KN.

15 de enero de 2018

In-depth resistome analysis by targeted metagenomics

Microbiome 6(1):11.

Antimicrobial resistance is a major global health challenge. Metagenomics allows analyzing the presence and dynamics of "resistomes" (the ensemble of genes encoding antimicrobial resistance in a given microbiome) in disparate microbial ecosystems. However, the low sensitivity and specificity of available metagenomic methods preclude the detection of minority populations (often present below their detection threshold) and/or the identification of allelic variants that differ in the resulting phenotype. Here, we describe a novel strategy that combines targeted metagenomics using last generation in-solution capture platforms, with novel bioinformatics tools to establish a standardized framework that allows both quantitative … Leer más

Lanza VF., Baquero F., Martinez JL., Ramos-Ruiz R., Gonzalez-Zorn B., Andremont A., Sanchez-Valenzuela A., Ehrlich SD., Kennedy S., Ruppe E., van Schaik W., Willems RJ., de la Cruz F. y Coque TM.

9 de enero de 2018

Genomic comparisons of Streptococcus suis serotype 9 strains recovered from diseased pigs in Spain and Canada

Veterinary Research 49(1):1.

     Streptococcus suis is one of the most important bacterial pathogens in the porcine industry and also a zoonotic agent. Serotype 9 is becoming one of the most prevalent serotypes within the S. suis population in certain European countries. In the present study, serotype 9 strains isolated from a country where infection due to this serotype is endemic (Spain), were compared to those recovered from Canada, where this serotype is rarely isolated from diseased pigs. For comparison purposes, strains from Brazil and the only strain isolated from a human case, in Thailand, were also incorporated. Firstly, sequence types (STs) were obtained followed by detection of putative virulence factors. Phylogenetic trees were constructed using the non-recombi… Leer más

Zheng H., Du P., Qiu X., Kerdsin A., Roy D., Bai X., Xu J., Vela AI. y Gottschalk M.

1 de enero de 2018

The relevance of host overcrowding in wildlife epidemiology: A new spatially explicit aggregation index

Ecological Indicators 84:695-700.

     Species distribution and population abundance are keystone patterns in ecology, and currently also in epidemiology. The aggregation of individuals in the population is closely related to distribution and abundance, but they are not totally equivalent patterns. Despite the great efforts made in recent decades to harmonise the sampling protocols used to collect distribution and abundance data, studies regarding the development and testing of aggregation indices are scarce, even when individuals’ aggregation is quite relevant and necessary for the design of effective wildlife management policies. One of the most popular aggregation indices is the overcrowding index (m*), which quantifies the number of individuals per group, and does not, there… Leer más

Laguna E., Barasona JA., Triguero-Ocana R., Mulero-Pazmany M., Negro JJ., Vicente J. y Acevedo P.

1 de enero de 2018

Recoding of synonymous genes to expand evolutionary landscapes requires control of secondary structure affecting translation

Biotechnology And Bioengineering 115(1):184-191.

     Synthetic DNA design needs to harness the many information layers embedded in a DNA string. We previously developed the Evolutionary Landscape Painter (ELP), an algorithm that exploits the degeneracy of the code to increase protein evolvability. Here, we have used ELP to recode the integron integrase gene (intI1) in two alternative alleles. Although synonymous, both alleles yielded less IntI1 protein and were less active in recombination assays than intI1. We spliced the three alleles and mapped the activity decrease to the beginning of alternative sequences. Mfold predicted the presence of more stable secondary structures in the alternative genes. Using synonymous mutations, we decreased their stability and recovered full activity. Followi… Leer más

Escudero JA., Nivina A., Cambray G., Lopez-Igual R., Loot C. y Mazel D.

Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ZTA 249 SUAT 61 SUAT 15
2 ICM 237 ZTA 52 MYC 11
3 MYC 227 MYC 48 SAP 11
4 SUAT 219 ICM 40 ZTA 5
5 NED 75 SAP 31 ICM 4
  VISAVET 1123 316 54

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.903 ZTA 5.366 ZTA 6.42
2 SAP 3.818 SUAT 4.227 SAP 6.06
3 SUAT 2.987 SAP 4.095 NED 4.3
4 MYC 2.8 NED 3.858 SUAT 4.287
5 DICM 2.776 ICM 3.798 MYC 3.118
VISAVET 3.282 4.869 6.827

ISI Scientific Publications
Total Last 60 mo.
1 Transbound Emerg Dis 93 Front Vet Sci 35
2 Vet Microbiol 88 Transbound Emerg Dis 32
3 Prev Vet Med 77 Animals 16
4 Front Vet Sci 61 Res Vet Sci 14
5 PLoS ONE 56 Sci Rep 14
  All journals 354   All journals 38