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Publicaciones científicas SCI 2021

10 de septiembre de 2021

The spatial ecology of red deer under different land use and management scenarios: Protected areas, mixed farms and fenced hunting estates

Science Of The Total Environment 786:147124.

     The knowledge regarding the spatial ecology of red deer (Cervus elaphus) in different environments is crucial if effective management actions are to be designed. However, this knowledge continues to be scarce in the complex contexts of mixed land use and management circumstances. This study describes the spatial ecology of red deer monitored using GPS collars in Mediterranean ecosystems of South-Central Spain, considering the effect of individual and seasonal (food shortage period, rut, hunting season and food abundance period) factors on different land use and management scenarios, namely protected areas, mixed farms and fenced hunting estates. Our results showed less activity (ACT), a shorter daily range (DR) and a smaller home range (HR)… Leer más

Laguna E., Carpio AJ., Vicente J., Barasona JA., Triguero-Ocana R., Jiménez-Ruiz S., Gomez-Manzaneque A. y Acevedo P.

1 de junio de 2021

Expansion of native wild boar populations is a new threat for semi-arid wetland areas

Ecological Indicators 125:107563.

     Wildlife management and conservation requires monitoring of species distribution and population indicators, especially when the unbalanced demographic changes of some species can affect the whole ecosystem functioning. The populations of wild boar (Sus scrofa) have, over the past few decades, undergone an expansion around the world, reaching situations of overabundance that can cause serious economic, ecological and health problems. This numerical increase of wild boar and its new spatial invasion can affect certain vulnerable species in sensitive ecological zones, such as the main inland wetland complex in SW Europe.
In this context, we aim to (i) examine the association of wild boar abundance and that of lagomorphs, and waterbird p… Leer más

Barasona JA., Carpio A., Boadella M., Gortazar C., Pineiro X., Zumalacarregui C., Vicente J. y Vinuela J.

30 de abril de 2021

Spatial and Temporal Distribution of Mycobacterium tuberculosis Complex Infection in Eurasian Badger (Meles meles) and Cattle in Asturias, Spain

Animals 11(5):1294.

     The present work investigated the prevalence, spatial distribution, and temporal distribution of tuberculosis (TB) in free-ranging Eurasian badgers (Meles meles) and cattle in Asturias (Atlantic Spain) during a 13-year follow-up. The study objective was to assess the role of badgers as a TB reservoir for cattle and other sympatric wild species in the region. Between 2008 and 2020, 673 badgers (98 trapped and 575 killed in road traffic accidents) in Asturias were necropsied, and their tissue samples were cultured for the Mycobacterium tuberculosis complex (MTC) isolation. Serum samples were tested in an in-house indirect P22 ELISA to detect antibodies against the MTC. In parallel, data on MTC isolation and single intradermal tuberculin test … Leer más

Blanco-Vazquez C., Doria-Barral T., Romero B., Queipo M., Merediz I., Quiros P., Armenteros JA., Juste R., Dominguez L., Dominguez M., Casais R. y Balseiro A.

19 de abril de 2021

The plasmid-mediated Kluyvera-like arnBCADTEF operon confers colistin (hetero) resistance to Escherichia coli

Antimicrobial Agents And Chemotherapy 65(5):e00091-21.

     The use of colistin as a last resort antimicrobial is compromised by the emergence of resistant enterobacteria with acquired determinants like mcr genes, mutations that activate the PmrAB system and by still unknown mechanisms. This work analyzed 74 E. coli isolates from healthy swine, turkey or bovine, characterizing their colistin resistance determinants. The mcr-1 gene, detected in 69 isolates, was the main determinant found among which 45% were carried by highly mobile plasmids, followed by four strains lacking previously known resistance determinants or two with mcr-4 (one in addition to mcr-1), whose phenotypes were not transferred by conjugation. Although a fraction of isolates carrying mcr-1 or mcr-4 genes also presented missense po… Leer más

Gallardo A., Iglesias M., Ugarte-Ruiz M., Hernandez M., Miguela-Villoldo P., Gutierrez G., Rodriguez-Lazaro D., Dominguez L. y Quesada A.

12 de abril de 2021

Population genomics and antimicrobial resistance dynamics of Escherichia coli in wastewater and river environments

Communications Biology 4(1):457.

     Aquatic environments are key niches for the emergence, evolution and dissemination of antimicrobial resistance. However, the population diversity and the genetic elements that drive the dynamics of resistant bacteria in different aquatic environments are still largely unknown. The aim of this study was to understand the population genomics and evolutionary events of Escherichia coli resistant to clinically important antibiotics including aminoglycosides, in anthropogenic and natural water ecosystems. Here we show that less different E. coli sequence types (STs) are identified in wastewater than in rivers, albeit more resistant to antibiotics, and with significantly more plasmids/cell (6.36 vs 3.72). However, the genomic diversity within E. … Leer más

Delgado-Blas JF., Ovejero CM., David S., Montero N., Calero-Caceres W., Garcillan-Barcia MP., de la Cruz F., Muniesa M., Aanensen DM. y Gonzalez-Zorn B..

10 de abril de 2021

Description and implementation of an On-farm Wildlife Risk Mitigation Protocol at the wildlife-livestock interface: tuberculosis in Mediterranean environments

Preventive Veterinary Medicine 191:105346.

     Animal tuberculosis (TB), which is caused by the Mycobacterium tuberculosis complex (MTC), is a zoonotic disease of global concern, and has a wide variety of wild and domestic reservoirs that can establish complex epidemiological systems. Of all the strategies employed to control TB, reducing the risks of interaction at the wildlife-livestock interface is a cornerstone. However, detailed protocols with which to assess and implement farm-specific preventive actions that can be employed against interactions with wildlife are lacking for extensive production systems. We describe an On-farm Wildlife Risk Mitigation Protocol that is applicable to beef cattle farming in Mediterranean environments in order to control the wildlife-livestock interac… Leer más

Martinez-Guijosa J., Lima-Barbero JF., Acevedo P., Cano-Terriza D., Jiménez-Ruiz S., Barasona JA., Boadella M., Garcia-Bocanegra I., Gortazar C. y Vicente J.

9 de abril de 2021

Effect of Biscuit Flour and Fermented Defatted Alperujo Co-Administration on Intestinal Mucosa Morphology and Productive Performance in Laying Hens

Animals 11:1075.

     In this study, the effects of co‐administration with biscuit flour and fermented defatted “alperujo” (FDA) on gut health were evaluated in a batch of laying hens (Hy‐Line 2015) on a commercial farm. Animals were divided into two groups: control group and treatment group; and histological and morphometric analyses of all sections of the intestine (duodenum, jejunum, ileum, cecum and rectum) were performed at 10, 18, 25, 50 and 75 weeks of age. During the whole productive period, a decrease in the mortality rate (p = 0.01) was observed in treated hens, as well as an increase in the number of eggs produced (p < 0.001), their size (p < 0.025), and weight (p < 0.024). In the early and late stages of production (10, 18 and 50 weeks), … Leer más

Porras-Gonzalez N., Rebollada A., Barcena C., Mayoral-Alegre F., Lomillos JM., Dominguez L. y Rodriguez-Bertos A..

7 de abril de 2021

Spoligotype-specific risk of finding lesions in tissues from cattle infected by Mycobacterium bovis

BMC Veterinary Research 17(1):148.

     Although the pathogenic effect of members of the Mycobacterium tuberculosis complex in susceptible hosts is well known, differences in clinical signs and pathological findings observed in infected animals have been reported, likely due to a combination of host and pathogen-related factors. Here, we investigated whether Mycobacterium bovis strains belonging to different spoligotypes were associated with a higher risk of occurrence of visible/more severe lesions in target organs (lungs and/or lymph nodes) from infected animals. A large collection of 8889 samples belonging to cattle were classified depending on the presence/absence of tuberculosis-like lesions and its degree of severity. All samples were subjected to culture irrespective of th… Leer más

Gómez-Buendía A., Romero B., Bezos J., Lozano F., de Juan L. y Alvarez J..

1 de abril de 2021

Polyresistant Mycobacterium bovis Infection in Human and Sympatric Sheep, Spain, 2017-2018

Emerging Infectious Diseases 27(4):1241-1243.

     The main etiologic agent of tuberculosis (TB) in livestock is Mycobacterium bovis; human TB cases caused by M. bovis are rare. Analysis of a TB outbreak caused by polyresistant M. bovis involving a human and sympatric sheep in Spain suggests local circulation of drug-resistant M. bovis strains among livestock… Leer más

Perez del Val B., Romero B., Tortola MT., Herrera-Leon L., Pozo P., Mercader I., Saez JL., Domingo M. y Vidal E.

1 de abril de 2021

Evaluation of the performance of slaughterhouse surveillance for bovine tuberculosis detection in Castilla y Leon, Spain

Preventive Veterinary Medicine 189:105307.

     Post-mortem inspection (PMI) of routinely slaughtered cattle in abattoirs is an extremely valuable tool for detecting bovine tuberculosis (bTB) infected herds that can supplement active surveillance activities. However, its true performance is difficult to assess due to the multiple factors that may affect it. Here, we determined relative efficiencies in the detection of bTB-compatible lesions and probabilities of subsequent laboratory confirmation of abattoirs located in Castilla y Leon, one of the regions with the largest cattle population in Spain, between 2010 and 2017. The slaughtered animal population was split based on the results of the ante-mortem tests (reactors or non-reactors), and two generalized linear multivariable mixed mode… Leer más

Pozo P., Cespedes-Cardenas N., Bezos J., Romero B., Grau A., Nacar J., Saez JL., Minguez O. y Alvarez J..

1 de abril de 2021

Antibiotic use in the COVID-19 crisis in Spain

Clinical Microbiology And Infection 27(4):646-647.

     Before the coronavirus disease 2019 (COVID-19) pandemic, antimicrobial resistance was the world`s most pressing health crisis. The United Nations, the World Health Organization (WHO) and the European Center for Disease Control and Prevention have made antimicrobial resistance a top priority, establishing a task force in the G-20 and organizing a joint program initiative on antimicrobial resistance including countries from al over the world [1]. However, with the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus pandemic in January 2020, all efforts are now focused on COVID-19, including controlling infections and developing vaccines… Leer más

Gonzalez-Zorn B.

30 de marzo de 2021

Towards a Better and Harmonized Education in Antimicrobial Stewardship in European Veterinary Curricula

Antibiotics 10(4):364.

     Education in antimicrobial stewardship (AMS) in veterinary medicine is essential to foster responsible antimicrobial use and control of antimicrobial resistance (AMR) in animals. AMS is listed by the EU and international organizations among the basic `Day One Competences` required of veterinary students upon graduation. Our aim was to evaluate the quality of education of European veterinary students in AMS. We distributed a 27-item survey addressing the perceptions of preparedness and acquired skills on key topics related to AMS to final-year veterinary students in Europe. We collected 3423 complete answers from 89 veterinary schools in 30 countries. Selection of treatment strategies and awareness of emerging AMR problems were markedly diff… Leer más

Espinosa-Gongora C., Rem Jessen L., James-Dyar O., Bousquet-Melou A., Gonzalez-Zorn B., Pulcini C., Re G., Schwarz S., Timofte D., Toutain PL., Guardabassi L. y PREPARE-VET Working Group.

17 de marzo de 2021

Transmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmer

Antibiotics 10(3):313.

     Colistin use has mostly been stopped in human medicine, due to its toxicity. However, nowadays, it still is used as a last-resort antibiotic to treat hospital infections caused by multi-drug resistant Enterobacteriaceae. On the contrary, colistin has been used in veterinary medicine until recently. In this study, 210 fecal samples from pigs (n = 57), calves (n = 152), and the farmer (n = 1) were collected from a farm where E. coli harboring mcr-1–mcr-3 was previously detected. Samples were plated, and mcr-genes presence was confirmed by multiplex-PCR. Hybrid sequencing which determined the presence and location of mcr-1, other antibiotic resistance genes, and virulence factors. Eighteen colistin resistant isolates (13 from calves, four from… Leer más

Vines J., Cusco A., Napp S., Alvarez J., Saez Llorente JL., Rosas-Rodoreda M., Francino O. y Migura-Garcia L.

10 de marzo de 2021

High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge

Vaccines 9:242.

     Abstract: African swine fever (ASF) is currently the major concern of the global swine industry, as a consequence of which a reconsideration of the containment and prevention measures taken to date is urgently required. A great interest in developing an effective and safe vaccine against ASF virus (ASFV) infection has, therefore, recently appeared. The objective of the present study
is to test an inactivated ASFV preparation under a vaccination strategy that has not previously been tested in order to improve its protective effect. The following have been considered: (i) virus inactivation by using a low binary ethyleneimine (BEI) concentration at a low temperature, (ii) the use of new and strong adjuvants; (iii) the use of very high … Leer más

Cadenas-Fernández E., Sanchez-Vizcaino JM., van den Born E., Kosowska A., van Kilsdonk E., Fernandez-Pacheco P., Gallardo C., Arias M. y Barasona JA..

8 de marzo de 2021

Globetrotting strangles: the unbridled national and international transmission of Streptococcus equi between horses

Microbial Genomics 00.

     The equine disease strangles, which is characterized by the formation of abscesses in the lymph nodes of the head and neck, is one of the most frequently diagnosed infectious diseases of horses around the world. The causal agent, Streptococcus equi subspecies equi , establishes a persistent infection in approximately 10 % of animals that recover from the acute disease. Such ‘carrier’ animals appear healthy and are rarely identified during routine veterinary examinations pre-purchase or transit, but can transmit S. equi to naïve animals initiating new episodes of disease. Here, we report the analysis and visualization of phylogenomic and epidemiological data for 670 isolates of S. equi recovered from 19 different countries using a new … Leer más

Mitchell C., Steward KF., Charbonneau ARL., Walsh S., Wilson H., Timoney JF., Wernery U., Joseph M., Craig D., van Maanen K., Hoogkamer-van Gennep A., Leon A., Witkowski L., Rzewuska M., Stefanska I., Zychska M., van Loon G., Cursons R., Patty O., Acke E., Gilkerson JR., El-Hage C., Allen J., Bannai H., Kinoshita Y., Niwa H., Becu T., Pringle J., Guss B., Bose R., Abbott Y., Katz L., Leggett B., Buckley TC., Blum SE., Cruz F., Fernadez-Ros A., Marotti Campi MC., Preziuso S., Robinson C., Newton JR., Schofield E., Brooke B., Boursnell M., de Brauwere N., Kirton R., Barton CK., Abudahab K., Taylor B., Yeats CA., Goater R., Aanensen DM., Harris SR., Parkhill J., Holden MTG. y Waller AS.

1 de marzo de 2021

Impact of nurse sows on influenza A virus transmission in pigs under field conditions

Preventive Veterinary Medicine 188:105257.

     Piglets prior to weaning play a central role in maintaining influenza infections in breeding herds and the use of nurse sows is a common practice to adopt piglets that fall behind and that otherwise would die. Transmission of influenza A virus (IAV) from nurse sows to adopted pigs has been reported experimentally, however, the importance of this route of transmission under field conditions has not yet been elucidated. A cohort study to assess the IAV status in nurse and control sows and their respective litters was carried out in three influenza positive breed-to-wean farms. A total of 94 control and 90 nurse sows were sampled by collecting udder skin wipes and oral swabs at enrollment (∼ 5–7 days after farrowing) and at weaning. Six … Leer más

Garrido Mantilla J., Sanhueza J., Alvarez J., Culhane M.R., Davies P., Allerson M.W. y Torremorel M.

1 de marzo de 2021

Efficacy of a Salmonella enterica serovar Abortusovis (S. Abortusovis) inactivated vaccine in experimentally infected gestating ewes

Research In Veterinary Science 135:486-494.

     Salmonella enterica serovar Abortusovis (S. Abortusovis) infection is one of the most important causes of infectious late-term abortion as well as birth of weak lambs in sheep in many countries throughout the world. Implementation of protocols based on the application of effective vaccines is one of the most effective approaches for controlling this disease, but variable efficacy has been reported, possibly related to factors associated with the host, the vaccine, the parameters used for determining efficacy and the challenge protocols. In this context, a new commercial inactivated vaccine (INMEVA; Laboratorios Hipra S.A., Spain) was evaluated in 20 control and 17 vaccinated gestating ewes, subcutaneously challenged at 90 days of gest… Leer más

Garcia-Seco T., Montbrau C., Fontseca M., March R., Sitja M., Dominguez L. y Bezos J..

5 de febrero de 2021

Assessing red deer hunting management in the Iberian Peninsula: the importance of longitudinal studies

PeerJ 00.

     Understanding the dynamics of a wildlife population in relation to hunting strategies is essential to achieve sustainable management. We used monitoring data over 25 years from two red deer (Cervus elaphus) populations with different management (with and without supplemental feeding) in South Central Spain to: (i) characterise the density dependence of population dynamics under contrasted management, and (ii) provide the basis for sustainable extraction by considering the theoretical maximum sustainable yield (MSYt) as the reference. The red deer population displayed a typical management reactive culling approach (‘saw-tooth-like’ curves), with occasional strong annual harvests but not occurring on a regular basis. Interestingly, we found r… Leer más

Carpio-Camargo AJ., Barasona JA., Acevedo O., Fierro Y., Gortazar C., Vigal C., Moreno A. y Vicente J.

27 de enero de 2021

African Swine Fever

Frontiers In Veterinary Science 7:632292.

     The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest… Leer más

Sanchez-Vizcaino JM., Laddomada A. y Martinez-Aviles M.

22 de enero de 2021

Use of Network Analysis and Spread Models to Target Control Actions for Bovine Tuberculosis in a State from Brazil

Microorganisms 9(2), 227.

     Livestock movements create complex dynamic interactions among premises that can be represented, interpreted, and used for epidemiological purposes. These movements are a very important part of the production chain but may also contribute to the spread of infectious diseases through the transfer of infected animals over large distances. Social network analysis (SNA) can be used to characterize cattle trade patterns and to identify highly connected premises that may act as hubs in the movement network, which could be subjected to targeted control measures in order to reduce the transmission of communicable diseases such as bovine tuberculosis (TB). Here, we analyzed data on cattle movement and slaughterhouse surveillance for detection of TB-l… Leer más

Cespedes-Cardenas N., Pozo P., Nunes Lopes FP., Grisi-Filho JHH. y Alvarez J..

15 de enero de 2021

Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System

Sensors 21:584.

     More effective methods to detect bovine tuberculosis, caused by Mycobacterium bovis, in wildlife, is of paramount importance for preventing disease spread to other wild animals, livestock, and human beings. In this study, we analyzed the volatile organic compounds emitted by fecal samples collected from free-ranging wild boar captured in Doñana National Park, Spain, with an electronic nose system based on organically-functionalized gold nanoparticles. The animals were separated by the age group for performing the analysis. Adult (>24 months) and sub-adult (12–24 months) animals were anesthetized before sample collection, whereas the juvenile (<12 months) animals were manually restrained while collecting the sample. Good accuracy was obtain… Leer más

Beleno-Saenz KJ., Caceres-Tarazona JM., Nol P., Jaimes-Mogollon AL., Gualdron-Guerrero OE., Duran-Acevedo CM., Barasona JA., Vicente J., Torres MJ., Welearegay TG., Osterlund L., Rhyan J. y Ionescu F.

Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ZTA 221 SUAT 71 ZTA 15
2 ICM 202 ZTA 58 SUAT 12
3 MYC 197 MYC 57 MYC 10
4 SUAT 195 ICM 31 SAP 5
  VISAVET 941 290 65

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.616 ZTA 4.215 ZTA 4.074
2 SAP 2.905 SAP 3.127 SEVISEQ 3.377
3 DICM 2.776 SUAT 2.917 SUAT 3.128
4 SUAT 2.634 DICM 2.776 ICM 3.038
5 MYC 2.565 ICM 2.722 MYC 2.999
VISAVET 2.804 3.209 4.265

ISI Scientific Publications
Total Last 60 mo.
1 Vet. Microbiol. 83 Transbound. Emerg. Dis. 49
2 Transbound. Emerg. Dis. 81 Front Vet Sci 38
3 Prev. Vet. Med. 73 PLoS ONE 24
4 PLoS ONE 54 Prev. Vet. Med. 22
5 Res. Vet. Sci. 46 Res. Vet. Sci. 18
  All journals 327   All journals 39