Publicaciones científicas SCI 2023
A useful tool for the safe diagnosis and control of the two main pandemics of the XXI century: COVID-19 and African Swine Fever disease
PLoS ONE 18(3):e0282632.
The COVID-19 pandemic and the disease triggered by the African Swine Fever virus are currently two of the main problems regarding public and animal health, respectively. Although vaccination seems to be the ideal tool for controlling these diseases, it has several limitations. Therefore, early detection of the pathogen is critical in order to apply preventive and control measures. Real-time PCR is the main technique used for the detection of both viruses, which requires previous processing of the infectious material. If the potentially infected sample is inactivated at the time of sampling, the diagnosis will be accelerated, impacting positively on the diagnosis and control of the disease. Here, we evaluated the inactivation and preservatio… Leer más
Barroso-Arevalo S., Diaz-de Frutos M., Kosowska A., Perez-Sancho M., Dominguez L. y Sanchez-Vizcaino JM..
A Maximum Entropy Species Distribution Model to Estimate the Distribution of Bushpigs on Madagascar and Its Implications for African Swine Fever
Transboundary And Emerging Diseases 7976252.
Bushpigs (Potamochoerus larvatus) play a major role in the socio-ecosystem of Madagascar, particularly in rural areas. They are largely hunted by rural populations as a major source of income and protein. They can also represent a potential source of pathogens for domestic animals and people. For example, it is hypothesized that bushpigs might compromise African swine fever (ASF) eradication programs by sporadically transmitting the virus to domestic pigs. However, available knowledge on the distribution of bushpigs in Madagascar is limited. In this study, we estimated the distribution of bushpigs on Madagascar using a species distribution model (SDM). We retrieved 206 sightings of bushpigs in Madagascar during 1990–2016 and predicted the d… Leer más
Diaz-Cao JM., Grossmann N., Goodman SM., Bosch J., Guis H., Rasamoelina M., Rakotoarivony R., Jori F. y Martinez-Lopez B.
Mycobacterium bovis in Egyptian mongoose, Spain
Zoonoses And Public Health 00:1-4.
Tuberculosis caused by Mycobacterium bovis and other related mycobacteria has been reported in a wide range of mammals worldwide. In the case of the Herpestidae family, Mycobacterium mungi and M. bovis, both belonging to the Mycobacterium tuberculosis Complex, have been reported in banded mongooses (Mungos mungo) in Africa and in Egyptian mongooses (Herpestes ichneumon) in Portugal, respectively. Thus, we hypothesized that Tuberculosis may occur in Egyptian mongooses from Spain. Twenty-five found dead Egyptian mongooses were necropsied in order to detect macroscopic TB-compatible lesions and mandibular lymph nodes and lungs were cultured onto mycobacteria-specific growth media. We isolated M. bovis in 3/25 Egyptian mongooses (12.00%, IC95: … Leer más
Ferreras-Colino E., Descalzo E., Romero B., Gortazar C. y Ferreras P.
Development of an Effective Oral Vaccine Dissemination Strategy against Classical Swine Fever for Wild Boar in Gifu Prefecture, Japan
Transboundary And Emerging Diseases 1-13.
In September 2018, classical swine fever (CSF) reemerged in Japan after more than a quarter of a century. After the first notification on a pig farm, wild boars positive for CSF were found continuously in the surrounding area. Gifu was the first prefecture in Japan to disseminate oral vaccines to wild boars in March 2019, with vaccines spread to approximately 14,000 sites between 2019 and 2020. While these diligent measures seemed to have shown some effectiveness, several vaccine spray sites remained without wild boar emergence. Based on the vaccine dissemination records from these periods, this study conducted a statistical analysis to propose more effective vaccine dissemination sites. First, a generalized linear mixed model was used to i… Leer más
Ito S., Bosch J., Aguilar-Vega C., Isoda N., Martinez-Aviles M. y Sanchez-Vizcaino JM..
Accuracy of Tests for Diagnosis of Animal Tuberculosis: Moving Away from the Golden Calf (and towards Bayesian Models)
Transboundary And Emerging Diseases 1-18.
Te last decades have seen major eforts to develop new and improved tools to maximize our ability to detect tuberculosis-infected animals and advance towards the objective of disease control and ultimately eradication. Nevertheless, there is still uncertainty regarding test performance due to the wide range of specifcity and especially sensitivity estimates published in the scientifc literature. Here, we performed a systematic review of the literature on studies that evaluated the performance of tuberculosis diagnostic tests used in animals through Bayesian Latent Class Models (BLCMs), which do not require the application of a (fallible) reference procedure to classify animals as infected with tuberculosis or not. BLCM-based sensitivity and … Leer más
Gómez-Buendía A., Pozo P., Picasso-Risso C., Branscum AJ., Perez A. y Alvarez J..
A tool to enhance antimicrobial stewardship using similarity networks to identify antimicrobial resistance patterns across farms
Scientific Reports 13(1):2931.
Antimicrobial resistance (AMR) is one of the major challenges of the century and should be addressed with a One Health approach. This study aimed to develop a tool that can provide a better understanding of AMR patterns and improve management practices in swine production systems to reduce its spread between farms. We generated similarity networks based on the phenotypic AMR pattern for each farm with information on important bacterial pathogens for swine farming based on the Euclidean distance. We included seven pathogens: Actinobacillus suis, Bordetella bronchiseptica, Escherichia coli, Glaesserella parasuis, Pasteurella multocida, Salmonella spp., and Streptococcus suis; and up to seventeen antibiotics from ten classes. A threshold crite… Leer más
Aguilar-Vega C., Scoglio C., Clavijo MJ., Robbins R., Karriker L., Liu X. y Martinez-Lopez B.
Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
Scientific Reports 13:2936.
The single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than Mycobacterium tuberculosis complex (MTC) can result in nonspecific reactions to these tests. We evaluated the performance of the skin test with PPDs and new defined antigens in the guinea pig model. A standard dose (SD) of Rhodococcus equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis, M. avium subsp. avium, M. avium subsp. hominissuis, M. scrofulaceum, M. persicum, M. microti, M. caprae and M. bovis, and a higher dose (HD) of M. nonchromogenicum… Leer más
Fernandez-Veiga L., Fuertes M., Geijo M., Perez del Val B., Vidal E., Michelet L., Boschiroli ML., Gómez-Buendía A., Bezos J., Jones GJ., Vordermeier M., Juste RA., Garrido JM. y Sevilla AI.
Portable Differential Detection of CTX-M ESBL Gene Variants, blaCTX-M-1 and blaCTX-M-15, from Escherichia coli Isolates and Animal Fecal Samples Using Loop-Primer Endonuclease Cleavage Loop-Mediated Isothermal Amplification
Microbiology Spectrum 11(1):e0331622.
Cefotaximase-Munich (CTX-M) extended-spectrum beta-lactamase (ESBL) enzymes produced by Enterobacteriaceae confer resistance to clinically relevant third-generation cephalosporins. CTX-M group 1 variants, CTX-M-1 and CTX-M-15, are the leading ESBL-producing Enterobacteriaceae associated with animal and human infection, respectively, and are an increasing antimicrobial resistance (AMR) global health concern. The blaCTX-M-1 and blaCTX-M-15 genes encoding these variants have an approximate nucleotide sequence similarity of 98.7%, making effective differential diagnostic monitoring difficult. Loop-primer endonuclease cleavage loop-mediated isothermal amplification (LEC-LAMP) enables rapid real-time multiplex pathogen detection with single-base … Leer más
Higgins O., Chueiri A., O´Connor L., Lahiff S., Burke L., Morris D., Pfeifer NM., Gonzalez-Santamarina B., Berens C., Menge C., Canica M., Manageiro V., Kisand V., Hassan MM., Gardner B., van Vliet AHM., La Ragione R., Gonzalez-Zorn B. y Smith TJ.
Treatment with the senolytics dasatinib/quercetin reduces SARS-CoV-2-related mortality in mice
Aging Cell e13771.
The enormous societal impact of the ongoing COVID-19 pandemic has been particularly harsh for some social groups, such as the elderly. Recently, it has been suggested that senescent cells could play a central role in pathogenesis by exacerbating the pro-inflammatory immune response against SARS-CoV-2. Therefore, the selective clearance of senescent cells by senolytic drugs may be useful as a therapy to ameliorate the symptoms of COVID-19 in some cases. Using the established COVID-19 murine model K18-hACE2, we demonstrated that a combination of the senolytics dasatinib and quercetin (D/Q) significantly reduced SARS-CoV-2-related mortality, delayed its onset, and reduced the number of other clinical symptoms. The increase in senescent markers… Leer más
Pastor-Fernandez A., Rodriguez-Bertos A., Sierra-Ramirez A., del Moral-Salmoral J., Merino J., de Avila AI., Olague C., Villares R., Gonzalez-Aseguinolaza G., Rodriguez MA., Fresno M., Girones N., Bustos M., Smerdou C., Fernandez-Marcos PJ. y von Kobbe C.
Engineered live bacteria suppress Pseudomonas aeruginosa infection in mouse lung and dissolve endotracheal-tube biofilms
Nature Biotechnology 000.
Engineered live bacteria could provide a new modality for treating lung infections, a major cause of mortality worldwide. In the present study, we engineered a genome-reduced human lung bacterium, Mycoplasma pneumoniae, to treat ventilator-associated pneumonia, a disease with high hospital mortality when associated with Pseudomonas aeruginosa biofilms. After validating the biosafety of an attenuated M. pneumoniae chassis in mice, we introduced four transgenes into the chromosome by transposition to implement bactericidal and biofilm degradation activities. We show that this engineered strain has high efficacy against an acute P. aeruginosa lung infection in a mouse model. In addition, we demonstrated that the engineered strain could dissolv… Leer más
Mazzolini R., Rodriguez-Arce I., Fernandez-Barat L., Pinero-Lambea C., Garrido V., Rebollada A., Motos A., Torres A., Grillo MJ., Serrano L. y Lluch-Senar M.
Characterization of the Fecal and Mucosa-Associated Microbiota in Dogs with Chronic Inflammatory Enteropathy
Animals 13:326.
Chronic inflammatory enteropathies are the most common cause of chronic vomiting and diarrhea in dogs. The pathogenesis of this disease is known to be multifactorial, where intestinal barrier dysfunction, immunological dysregulation and gut microbiota changes play a central role. Most sequencing studies assessing the intestinal microbiota in canine species have been made to evaluate fecal samples. Therefore, in this study, we aimed to characterize the intestinal bacterial microbiota from duodenal biopsies and fecal samples in dogs with inflammatory bowel disease at the diagnosis time and to compare it to healthy dogs. Our study results demonstrate that
dogs with inflammatory bowel disease have significantly different gut microbiota w… Leer más
Diaz-Reganon D., Garcia-Sancho M., Villaescusa A., Sainz A., Agulla B., Reyes-Prieto M., Rodriguez-Bertos A. y Rodriguez-Franco F.
Struggling to improve farm biosecurity: Do free advice and subsidies hit the target?
Preventive Veterinary Medicine 212:105839.
Biosafety measures (BSMs) often aim at reducing the likelihood of cross-species interactions at the wildlife-livestock interface. Examples include means to segregate wild ungulates from cattle at waterholes or at feeders. Subsidies or incentives for BSM implementation are expected to contribute to improved BSM acceptance. However, several recent experiences led us to write a cautionary commentary on the variable success of incentives in farm biosafety promotion. We list examples where, after offering farm-specific biosecurity action plans for free or subsidizing 100% of the cost of a given BSM, 25-40% of the farmers remained unwilling to invest efforts in farm biosafety and BSM maintenance. We suggest seeking a better understanding of farme… Leer más
Preite L., Barroso P., Romero B., Balseiro A. y Gortazar C.
What about the bull? A systematic review about the role of males in bovine infectious infertility within cattle herds
Veterinary And Animal Science 19:100284.
Numerous pathogens affect cow fertility. Nevertheless, little information has been published about microorganisms associated with cattle infertility focusing on bulls. The present review offers a current analysis and highlights potential key aspects on the relevance of bulls in the emergence of infertility problems of infectious origin within herds that are still not completely determined. The present systematic review was conducted using the PubMed, Web of Science, and Scopus databases on December 9, 2022. In total, 2,224 bibliographic records were reviewed and, according to strict inclusion criteria, 38 articles were selected from 1966 to 2022, from which we ranked more than 27 different microorganisms (fungi were not identified). The mos… Leer más
Polo C., Garcia-Seco T., Diez-Guerrier A., Briones V., Dominguez L. y Perez-Sancho M..
First report and molecular characterization of cases of natural Taylorella asinigenitalis infection in three donkey breeds in Spain
Veterinary Microbiology 276:109604.
Taylorella asinigenitalis is a non-pathogenic bacteria isolated from the genital tract of donkeys but also a cause of metritis and vaginal discharge in mares. It is closely related to Taylorella equigenitalis, the cause of Contagious Equine Metritis (CEM) in horses, and has been present in different countries in Europe since 1995. Up to date, there are no studies on the prevalence of T. asinigenitalis in the equine or asinine populations in Spain; this is the first report of the presence of T. asinigenitalis in donkeys (Equus asinus) from different breeds in three regions of Spain. A total of 106 healthy animals of three different Spanish donkey breeds: Andaluza (26), Majorera (12) and Zamorano-Leonés (68) were sampled between June and July… Leer más
Dorrego-Rodriguez A., Herranz-Benito C., Perez-Sancho M., Camino E., Gomez-Arrones V., Carrasco JJ., de Gabriel-Perez J., Serres C. y Cruz F..
