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Immunopathology of early and advanced epididymis lesions caused by Brucella ovis in rams

Artículo de investigación publicado en Veterinary Immunology and Immunopathology

1 de julio de 2023

Ovine brucellosis is an infectious disease that causes alterations in the reproductive tract in ram and abortion in ewes. Their negative economic impact in ovine production warrants a thorough understanding the interactions between B. ovis and the host. Here, epididymis lesions of rams infected by B. ovis were histopathologically staged into early and advanced. Expression by immunohistochemistry of Brucella antigens, inflammatory cell markers (CD3, CD79αcy) and cytokines (IFN-γ, TNF-α, TGF-β1) was assessed in both stages. Early lesions were characterized by epithelial changes, interstitial inflammation, and mild fibrosis; whereas advanced lesions displayed caseous granulomas containing numerous macrophages, multinucleated giant cells, lymphocytes, and plasma cells. Expression of Brucella antigens were observed in both stages. The cellular response in B. ovis lesions were predominantly of T-cells (CD3+) whereas low numbers of B-cells and plasma cells (CD79αcy+) were present in both early and advanced lesions. IFN-γ was expressed by lymphocytes in early lesions suggesting that the adaptive immune response against B. ovis is initiated by Th1 cells, this response was also preserved in advanced stages. Expression of TNF-α was observed in neutrophils of epithelial microabscesses and intraepithelial T-cells of early lesions suggesting a promotion of neutrophil phagocytosis triggered by TNF-α. On the other hand, advanced lesions showed a reduction of TNF-α expression which may permit B. ovis persistence in granulomas. Lastly, TGF-β1 expression (fibroblast, macrophages and less in lymphocytes) were increased with time, suggesting that B. ovis promotes TGF-β1 secretion promoting chronicity of the lesions




Rebollada A., Garcia-Seco T., Chinchilla-Rodríguez B., Perez-Sancho M., Dominguez L. y Rodriguez-Bertos A..




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Immunopathology of early and advanced epididymis lesions caused by Brucella ovis in rams

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Immunopathology of early and advanced epididymis lesions caused by Brucella ovis in rams



Participantes:

Universidad ComplutenseServicio de Patología y Veterinaria Forense (SAP). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Medicina y Cirugía Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Veterinary Immunology and Immunopathology
FACTOR YEAR Q
1.400 2023

NLMID: 8002006

PMID: 37348444

ISSN: 0165-2427



TÍTULO: Immunopathology of early and advanced epididymis lesions caused by Brucella ovis in rams


REVISTA: Vet Immunol Immunopathol


NUMERACIÓN: 261:110621


AÑO: 2023


EDITORIAL: Elsevier Scientific


AUTORES: Rebollada A., Garcia-Seco T., Chinchilla-Rodríguez B., Perez-Sancho M., Dominguez L. and Rodriguez-Bertos A..


2nd
María Teresa García-Seco Romero
3rd
Blanca Chinchilla Rodríguez
4th
Marta Pérez Sancho
5th
Lucas Domínguez Rodríguez
Last
Antonio Manuel Rodríguez Bertos

DOI: https://doi.org/10.1016/j.vetimm.2023.110621


CITA ESTA PUBLICACIÓN:

Rebollada A., Garcia-Seco T., Chinchilla-Rodríguez B., Perez-Sancho M., Dominguez L. y Rodriguez-Bertos A. Immunopathology of early and advanced epididymis lesions caused by Brucella ovis in rams. Veterinary Immunology and Immunopathology. 261:110621. 2023. (A). ISSN: 0165-2427. DOI: 10.1016/j.vetimm.2023.110621


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