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Publicaciones científicas SCI 2024

1 de junio de 2024

Mycobacterium tuberculosis complex in domestic goats in Southern Spain

Preventive Veterinary Medicine 227:106204.

     Tuberculosis (TB) is a zoonotic infectious disease caused by bacteria belonging to the Mycobacterium tuberculosis complex (MTC), which can affect a wide variety of domestic and wild animal species. Although the role of goats as a reservoir of MTC bacteria has been evidenced, information about the circulation of MTC strains in this species is still very scarce. The aim of the present study was to determine the seroprevalence, spatial distribution, risk factors and MTC spoligotypes circulating in goats from Andalusia (Southern Spain), the Spanish region with the largest goat census and a hotspot area of TB in both cattle and wild ungulates. A total of 2155 serum samples from 80 goat flocks were analyzed by an in-house ELISA using the P22 prot… Leer más

Jimenez-Martin D., Garcia-Bocanegra I., Risalde MA., Napp S., Dominguez M., Romero B., Moreno I., Martinez R. y Cano-Terriza D.

1 de mayo de 2024

Detection of equine herpesvirus-1 (EHV-1) in urine samples during outbreaks of equine herpesvirus myeloencephalopathy

Equine Veterinary Journal 56(3):456-463.

     Background: Real-time PCR is the diagnostic technique of choice for the diagnosis and control of equine herpesvirus-1 (EHV-1) in an outbreak setting. The presence of EHV-1 in nasal swabs (NS), whole blood, brain and spinal cord samples has been extensively described; however, there are no reports on the excretion of EHV-1 in urine, its DNA detection patterns, and the role of urine in viral spread during an outbreak.
Objectives: To determine the presence of EHV-1 DNA in urine during natural infection and to compare the DNA detection patterns of EHV-1 in urine, buffy coat (BC) and NS.
Study design: Descriptive study of natural infection.
Methods: Urine and whole blood/NS samples were collected at different time points dur… Leer más

Velloso-Alvarez A., Jose-Cunilleras E., Dorrego-Rodriguez A., Santiago-Llorente I., de las Cuesta-Torrado M., Troya-Portillo L., Rivera B., Vitale V., de Juan L. y Cruz F..

2 de abril de 2024

Active surveillance of antimicrobial resistance in companion animals: A pilot study in a Spanish Veterinary Teaching Hospital

Comparative Immunology, Microbiology And Infectious Diseases 108:102169.

     The role of small animal veterinary hospitals in the onset and dissemination of antimicrobial-resistant organisms (AMROs) is still not clear, and the implementation of an internal surveillance systems is a cost-effective tool to better understand their impact. The aim of this study was to describe a pilot program of active surveillance in a Spanish Veterinary Teaching Hospital, developed to estimate the detection frequency of AMROs in the commensal flora of patients and in the environment. Surveillance was focused on Methicillin-resistant Staphylococci (MRS), third generation cephalosporins resistant gram-negative bacteria (3GCR-GNB), and carbapenems-resistant gram-negative bacteria (CR-GNB). Oral and perirectal swabs were collected in the … Leer más

Scarpellini R., Leal Velez de Mendizábal L., Quevedo-Caraballo S., Blanco JL., Garcia ME., Perez-Sancho M., Portero-Fuentes M., Penelo S., Esposito E., Mondo E. y Piva S.

2 de abril de 2024

Forensic findings in urban red fox mortality in the metropolitan area of Madrid, 2014-2022

Journal Of Veterinary Diagnostic Investigation 1-5.

     The expanding presence of red foxes (Vulpes vulpes) in urban and suburban regions could potentially lead to increased instances of human aggression towards this species. We studied 10 deceased red foxes that were submitted by law enforcement agencies in the metropolitan area of Madrid in 2014-2022 because of suspected abuse. Forensic autopsies were performed to establish the cause and manner of death. In 4 of the 10 cases, the cause of death was deemed unnatural, involving blunt-force trauma (n = 2), asphyxia resulting from hanging (n = 1), and firearm injury (n = 1). Among the remaining cases, most had succumbed to natural causes (n = 4), often marked by severe emaciation and a high burden of parasites, primarily Sarcoptes scabiei. In 2 ca… Leer más

Rebollada A., Barcena C., Porras N., Dominguez L. y Rodriguez-Bertos A..

27 de marzo de 2024

Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus

The Veterinary Quarterly 44(1):1-13.

     Despite the great interest in the development of a vaccine against African swine fever (ASF) in wild boar, the immunological mechanisms that induce animal protection are still unknown. For this purpose, tertiary lymphoid organs (TLOs) of wild boar were characterised and compared with mucosa-associated lymphoid tissues (MALTs) by histopathology, histomorphometry and immunohistochemistry (CD3, CD79, PAX5, LYVE1, fibronectin). In addition, real-time polymerase chain reaction (qPCR) and immunohistochemistry (p72) were used to evaluate the presence of ASF virus (ASFV) in blood and tissues samples, respectively. TLOs were observed in animals infected with a low-virulent ASFV isolate (LVI), animals co-infected with low and high-virulent ASFV isola… Leer más

Porras N., Sanchez-Vizcaino JM., Rodriguez-Bertos A., Kosowska A. y Barasona JA..

22 de marzo de 2024

Eco-Sanitary Regionalization of Wild Boar (Sus scrofa) in the Western Palearctic Realm as a Tool for the Stewardship of African Swine Fever

Transboundary And Emerging Diseases 8080496.

     African swine fever (ASF) is a viral hemorrhagic disease affecting domestic and wild suids, with catastrophic socioeconomic impact worldwide. In the European scenario, wild boar (Sus scrofa) actively contributes to the maintenance and spread of the disease. In this study, we applied a multivariate clustering method to define ecoregions in the western Palearctic realm based on ecological and sanitary aspects of ASF in wild boar. Beforehand, we performed a principal components analysis for the eight selected variables. An analysis of the countries was carried out in terms of the extent of ecoregions and ASF notifications. After clustering, we identified nine eco-regions that showed important differences based on the used eco-sanitary variable… Leer más

Aguilar-Vega C., Munoz-Perez C., Sanchez-Vizcaino JM., Martinez-Aviles M. y Bosch J.

9 de marzo de 2024

Molecular detection of herpesvirus in a skin lesion of a humpback whale (Megaptera novaeangliae) from the Western Mediterranean Sea

European Journal Of Wildlife Research 70:31.

     Herpesvirus has the potential to infect a wide variety of animal species. In cetaceans, Alpha- and/or Gammaherpesvirinae have been identified in eight families of odontocetes, and one family of mysticetes. In May 2022, an adult humpback whale (Megaptera novaeangliae) was found stranded in Valencia, Spain. The whale was emaciated, in poor body condition, with multiple lacerations on the dorsal fin and a high number of epibionts of the Cyamidae family, known as whale lice. The individual had been previously released from a ghost net entanglement 5 days before becoming stranded. In a closer examination, various skin lesions were observed, including chronic, proliferative, and erosive dermatitis and a large ulcer extending to the deep dermis. A… Leer más

Vargas-Castro I., Crespo-Picazo JL., Jimenez-Martinez MA., Munoz-Baquero M., Marco-Cabedo V., Garcia-Parraga D. y Sanchez-Vizcaino JM..

8 de marzo de 2024

Pancreatic adenocarcinoma treated with surgical resection, toceranib phosphate and firocoxib in a dog: a case report

Veterinary Research Communications 1-7.

     Exocrine pancreatic carcinomas are rarely reported in dogs. A ductal pancreatic adenocarcinoma in a 10-year-old intact beagle is described in this report. The diagnosis was made based on clinical signs, imaging (abdominal ultrasound and CT scan) and histopathology. Treatment consisted of partial right lobe pancreatectomy followed by adjuvant therapy with toceranib phosphate (Palladia®) and firocoxib (Previcox®) for six months. The treatment was well tolerated, and the survival time was 445 days. To our knowledge, this is the longest survival reported in the literature for a dog diagnosed with exocrine pancreatic adenocarcinoma. The results described here may contribute to provide a better understanding about this neoplasia and potential tre… Leer más

Fuertes-Recuero M., Vazquez-Fernandez E., Lizasoain-Sanz G., Arroba-Alonso A., Sanchez-Lopez A., Martinez-de Merlo E., Suarez-Redondo M. y Ortiz-Diez G.

4 de marzo de 2024

Application of machine learning with large-scale data for an effective vaccination against classical swine fever for wild boar in Japan

Scientific Reports 14(1):5312.

     Classical swine fever has been spreading across the country since its re-emergence in Japan in 2018. Gifu Prefecture has been working diligently to control the disease through the oral vaccine dissemination targeting wild boars. Although vaccines were sprayed at 14,000 locations between 2019 and 2020, vaccine ingestion by wild boars was only confirmed at 30% of the locations. Here, we predicted the vaccine ingestion rate at each point by Random Forest modeling based on vaccine dissemination data and created prediction surfaces for the probability of vaccine ingestion by wild boar using spatial interpolation techniques. Consequently, the distance from the vaccination point to the water source was the most important variable, followed by elev… Leer más

Ito S., Aguilar-Vega C., Bosch J., Isoda N. y Sanchez-Vizcaino JM..

28 de febrero de 2024

Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats

Veterinary Sciences 11(3):105.

     Paratuberculosis (PTB) and tuberculosis (TB) are two mycobacterial diseases with a severe economic and health impact on domestic ruminants. The ante mortem diagnosis of PTB is hampered, among other factors, by the limited sensitivity of all the available diagnostic techniques. Since TB-infected goats subjected to the comparative intradermal tuberculin test (CITT) may experience a booster effect on their antibody titer and a potential enhancement to the sensitivity of humoral techniques for tuberculosis, in the present study we aimed to evaluate this diagnostic strategy on the humoral diagnosis of PTB in serum and milk samples collected from a caprine herd that was TB free and PTB infected. The results from 120 goats indicated a significant … Leer más

Velasco-Reinaldos C., Ortega J., Gómez-Buendía A., Grau A., Lopez M., Alvarez J., Romero B., de Juan L. y Bezos J..

16 de febrero de 2024

Phenotypic and genetic characterization of antimicrobial resistance in Salmonella enterica serovar Choleraesuis isolates from humans and animals in Spain from 2006 to 2021

The Journal Of Antimicrobial Chemotherapy 1-11.

     Objectives
While an increase in the levels of MDR in Salmonella enterica sevorar Choleraesuis has been reported in Europe, little is known about the situation in Spain. Therefore, we first aimed to assess the phenotypic resistance profile and to determine the presence of genetic determinants of resistance of S. Choleraesuis isolates collected in animal and human. Our second objective was to identify and characterize clusters of highly related isolates.

Methods
We analysed 50 human and 45 animal isolates retrieved from 2006 to 2021 using the disc diffusion method and performed WGS followed by analyses of genetic determinants and phylogenetic analysis.

Results
All isolates were of ST145 and c… Leer más

Jacqueline C., Samper-Cativiela C., Monzon S., Ugarte-Ruiz M., Cuesta I., Alvarez J. y Herrera-Leon S.

12 de febrero de 2024

Landscape connectivity for predicting the spread of ASF in the European wild boar population

Scientific Reports 10;14(1):3414.

     African swine fever (ASF) is an infectious and highly fatal disease affecting wild and domestic swine, which is unstoppably spreading worldwide. In Europe, wild boars are one of the main drivers of spread, transmission, and maintenance of the disease. Landscape connectivity studies are the main discipline to analyze wild-species dispersal networks, and it can be an essential tool to predict dispersal-wild boar movement routes and probabilities and therefore the associated potential ASF spread through the suitable habitat. We aimed to integrate wild boar habitat connectivity predictions with their occurrence, population abundance, and ASF notifications to calculate the impact (i.e., the capacity of a landscape feature to favor ASF spread) an… Leer más

Goicolea T., Cisneros-Araujo P., Aguilar-Vega C., Sanchez-Vizcaino JM., Mateo-Sanchez MC. y Bosch J.

12 de febrero de 2024

Forensic investigations of suspected livestock depredation by vultures: scientific tools for compensation programmes

Journal Of Comparative Pathology 209:22-30.

     Human–wildlife conflicts may have devastating consequences for fauna due to targeting by humans of wildlife populations suspected to have predated livestock. Suspicion of depredation of extensively raised livestock by vultures in Europe has triggered public administration-led forensic investigations intended to distinguish between predation and scavenging in order to compensate farmers for attacks on their livestock. In this study, gross and histological analyses were carried out on suspected cases of domestic animal depredation by griffon vultures (Gyps fulvus) over a 1-year period. Fifty-eight animals were affected (n = 41, suspected depredation), including domestic cattle (75.9%), sheep (22.4%) and a goat (1.7%). All the adults affected … Leer más

Rebollada A., Gómez-Buendía A., Dominguez L. y Rodriguez-Bertos A..

8 de febrero de 2024

Effect of a recent intradermal test on the specificity of P22 ELISA for the diagnosis of caprine tuberculosis

Frontiers In Veterinary Science 11:1-7.

     Caprine tuberculosis (TB) is a zoonotic disease caused by members of the Mycobacterium tuberculosis complex. TB eradication programs in goats are based on the single and comparative intradermal tuberculin tests (SITT and CITT, respectively). Antibody-based diagnostic techniques have emerged as potential diagnostic tools for TB. P22 ELISA has been previously evaluated using samples collected after the intradermal tuberculin tests to maximize the sensitivity, a phenomenon known as booster effect. However, there is no information available on whether the use of this diagnostic strategy could lead to a decrease of its specificity (Sp). The aim of the present study was to elucidate the interference effect of a recent CITT on the Sp of the P22 EL… Leer más

Velasco-Reinaldos C., Ortega J., Rincón-Fernández de la Puente J., Romero B., de Juan L., Dominguez L., Dominguez M., Moreno I., Alvarez J. y Bezos J..

31 de enero de 2024

Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds

Frontiers In Veterinary Science 11:1361788.

     Non-tuberculous mycobacteria (NTM) are considered a relevant cause of non-specific reactions to the most widely applied bovine tuberculosis (bTB) test, the intradermal tuberculin test. In order to establish which NTM species might act as a potential source of such diagnostic interference, a collection of 373 isolates obtained from skin test positive cows from 359 officially tuberculosis-free (OTF) herds, culled in the framework of the bTB eradication campaign in Spain, were identified at the species level through PCR and Sanger sequencing of the 16S rDNA, hsp65 and rpoB genes. Of the 308 isolates for which a reliable identification was achieved, 32 different mycobacterial species were identified, with certain species being most represented:… Leer más

Gómez-Buendía A., Alvarez J., Bezos J., Mourelo J., Amado J., Saez-Llorente JL., de Juan L. y Romero B..

16 de enero de 2024

Detection of Brucella in Dermacentor ticks of wild boar with brucellosis

Transboundary And Emerging Diseases 2024:5517000, 12 pag.

     Brucellosis is a sanitary and economically relevant disease affecting humans, livestock, and wildlife. Ticks have been suggested as vectors, long-term carriers, and amplifiers of Brucella. In this study, ticks from wildlife ungulate hosts living in hunting reserves of a central region of Spain were collected during a 6-year period, pooled, and screened for Brucella spp. by PCR. Aiming to correlate Brucella spp. DNA presence in ticks with Brucella spp. infections in wildlife ungulate hosts, liver samples from deceased wildlife ungulates coming from the hunting reserves showing a positive result for Brucella in ticks were tested using a commercial ELISA. In total, 229 tick pools from wild boar (Sus scrofa, n = 176; 76.8%, 95% CI 7… Leer más

Rebollada A., Martinez I., Duque C., Garcia-Seco T., Escacena C., Dominguez L., Rodriguez-Bertos A. y Garcia N..

12 de enero de 2024

Identification of promoter activity in gene-less cassettes from Vibrionaceae superintegrons

Nucleic Acids Research 1-16.

     Integrons are genetic platforms that acquire new genes encoded in integron cassettes (ICs), building arrays of adaptive functions. ICs generally encode promoterless genes, whose expression relies on the platform-associated Pc promoter, with the cassette array functioning as an operon-like structure regulated by the distance to the Pc. This is relevant in large sedentary chromosomal integrons (SCIs) carrying hundreds of ICs, like those in Vibrio species. We selected 29 gene-less cassettes in four Vibrio SCIs, and explored whether their function could be related to the transcription regulation of adjacent ICs. We show that most gene-less cassettes have promoter activity on the sense strand, enhancing the expression of downstream cassettes. Ad… Leer más

Blanco P., Hipolito A., Garcia-Pastor L., Trigo de Roza F., Toribio-Celestino L., Ortega AC., Vergara E., San Millan A. y Escudero JA..

6 de enero de 2024

Heat-inactivated mycobacteria activate the toll-like receptor 2 and 4 pathways in the zebrafish model of tuberculosis

Vaccine S0264-410X(23)01544-X.

     Based on previous evidence demonstrating the efficacy of inactivated mycobacteria for the control of fish mycobacteriosis, we explored the protective efficacy of two inactivated Mycobacterium bovis administered via parenteral and mucosal routes against Mycobacterium marinum infection mimicking natural conditions in the zebrafish model of tuberculosis. Although we did not observe a clear effect of any of the immunostimulants on mycobacterial burden, the results showed a significant increase in TLR2 and TLR4 gene expression levels in fishes parenterally immunized with inactivated Bacillus Calmette-Guérin (BCG). Our findings demonstrated that the TLR2 and the TLR4 signaling pathways are involved in the immune response elicited by inactivated m… Leer más

Ferreras E., Contreras M., Risalde MA., Sevilla IA., Delgado E., Dominguez L., Gortazar C. y de la Fuente J.






Science Publication
Indicators
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ZTA 248 SUAT 64 SUAT 16
2 ICM 237 ZTA 52 MYC 11
3 MYC 227 MYC 49 SAP 11
4 SUAT 219 ICM 42 ZTA 5
5 NED 75 SAP 31 ICM 5
  VISAVET 1121 321 55

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 SAP 3.898 ZTA 5.366 ZTA 6.42
2 ZTA 3.875 SUAT 4.187 SAP 6.109
3 SUAT 2.987 SAP 4.172 NED 4.3
4 MYC 2.8 NED 3.858 SUAT 4.25
5 DICM 2.776 ICM 3.769 MYC 3.118
VISAVET 3.278 4.848 6.822

ISI Scientific Publications
Total Last 60 mo.
1 Transbound Emerg Dis 93 Front Vet Sci 36
2 Vet Microbiol 88 Transbound Emerg Dis 33
3 Prev Vet Med 77 Animals 16
4 Front Vet Sci 61 Res Vet Sci 14
5 PLoS ONE 56 Sci Rep 14
  All journals 354   All journals 37