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Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level

Investigación publicada en Journal of Fish Diseases

1 de octubre de 2018

The aim of this study was to evaluate the usefulness of the MALDI-TOF MS to identify 151 isolates of Aeromonas obtained mostly from diseased fish. MALDI-TOF MS correctly identified all isolates to the genus level but important differences in the percentage of isolates correctly identified depending on the species were observed. Considering exclusively the first identification option, Aeromonas bestiarum, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas veronii and Aeromonas sobria were the best identified with results >95%. However, considering the first and second identification options, the only species that showed values >90% was A. hydrophila. Overall, when the database was supplemented with 14 new spectra, the number of accurate identifications increased (41% vs. 55%) and the number of inconclusive identifications decreased (45% vs. 29%), but great differences in the success of species-level identifications were found. Species-distinctive mass peaks were identified only for A. hydrophila and A. bestiarum (5003 and 7360 m/z in 95.5% and 94.1% of their isolates, respectively). This work demonstrates the utility of MALDI-TOF MS for Aeromonas identification to the genus level, but there is no consistency for the accurate identification of some of the most prevalent species implicated in fish disease




Perez-Sancho M., Cerda I., Fernandez-Bravo A., Dominguez L., Figueras MJ., Fernandez-Garayzabal JF. y Vela AI..




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Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level

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Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level


Participantes:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universitat Rovira i VirgiliUnidad de Microbiología. Facultad de Medicina y Ciencias de la Salud. Universitat Rovira i Virgili (URV).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Journal of Fish Diseases
FACTOR YEAR Q
1.988 2018

NLMID: 9881188

PMID: 30105821

ISSN: 0140-7775



TÍTULO: Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level


REVISTA: J. Fish Dis.


NUMERACIÓN: 41(10):1485-1493


AÑO: 2018


EDITORIAL: Blackwell Scientific Publications


AUTORES: Perez-Sancho M., Cerda I., Fernandez-Bravo A., Dominguez L., Figueras MJ., Fernandez-Garayzabal JF. and Vela AI..


PARTICIPANTES VISAVET


First
Marta Pérez Sancho
4th
Lucas Domínguez Rodríguez
6th
José Francisco Fernández-Garayzabal Fernández
Last
Ana Isabel Vela Alonso

DOI: https://doi.org/10.1111/jfd.12837


CITA ESTA PUBLICACIÓN:

Perez-Sancho M., Cerda I., Fernandez-Bravo A., Dominguez L., Figueras MJ., Fernandez-Garayzabal JF. y Vela AI. Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level. Journal of Fish Diseases. 41(10):1485-1493. 2018. (A). ISSN: 0140-7775. DOI: 10.1111/jfd.12837


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