Inicio \ Investigación \ Publicaciones científicas \


Ant(6)-I genes encoding aminoglycoside O-nucleotidyltransferases are widely spread among streptomycin resistant strains of Campylobacter jejuni and Campylobacter coli

Frontiers in Microbiology publica este artículo de investigación

23 de octubre de 2018

Thermotolerant Campylobacter species C. jeuni and C. coli are actually recognized as the major bacterial agent responsible for food-transmitted gastroenteritis. The most effective antimicrobials against Campylobacter are macrolides and some, but not all aminoglycosides. Among these, susceptibility to streptomycin is reduced by mutations in the ribosomal RPSL protein or by expression of ANT(6)-I aminoglycoside O-nucleotidyltransferases. The presence of streptomycin resistance genes was evaluated among streptomycin-resistant Campylobacter isolated from humans and animals by using PCR with degenerated primers devised to distinguish ant(6)-Ia, ant(6)-Ib and other ant-like genes. Genes encoding ANT(6)-I enzymes were found in all possible combinations with a major fraction of the isolates carrying a previously described ant-like gene, distantly related and belonging to the new ant(6)-I sub-family ant(6)-Ie. Among Campylobacter isolates, ant(6)-Ie was uniquely found functional in C. coli, as shown by gene transfer and phenotype expression in Escherichia coli, unlike detected coding sequences in C. jejuni that were truncated by an internal frame shift associated to RPSL mutations in streptomycin resistant strains. The genetic relationships of C. coli isolates with ANT(6)-Ie revealed one cluster of strains presented in bovine and humans, suggesting a circulation pathway of Campylobacter strains by consuming contaminated calf meat by bacteria expressing this streptomycin resistance element




Hormeno L., Ugarte-Ruiz M., Palomo G., Borge C., Vadillo S., Piriz S., Dominguez L., Campos MJ. y Quesada A.




Ver artículo
Ant(6)-I genes encoding aminoglycoside O-nucleotidyltransferases are widely spread among streptomycin resistant strains of Campylobacter jejuni and Campylobacter coli

Ver en NLM PubMed
Ant(6)-I genes encoding aminoglycoside O-nucleotidyltransferases are widely spread among streptomycin resistant strains of Campylobacter jejuni and Campylobacter coli

Participantes:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Frontiers in Microbiology
FACTOR YEAR Q
4.109 2017

NLMID: 101548977

PMID: 30405573

ISSN: 1664-302X



TÍTULO: Ant(6)-I genes encoding aminoglycoside O-nucleotidyltransferases are widely spread among streptomycin resistant strains of Campylobacter jejuni and Campylobacter coli


REVISTA: Front Microbiol


NUMERACIÓN: 9:2515


AÑO: 2018


EDITORIAL: Frontiers Research Foundation


AUTORES: Hormeno L., Ugarte-Ruiz M., Palomo G., Borge C., Vadillo S., Piriz S., Dominguez L., Campos MJ. and Quesada A.


PARTICIPANTES VISAVET


2nd
María Ugarte Ruiz
7th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.3389/fmicb.2018.02515


CITA ESTA PUBLICACIÓN:

Hormeno L., Ugarte-Ruiz M., Palomo G., Borge C., Vadillo S., Piriz S., Dominguez L., Campos MJ. y Quesada A. Ant(6)-I genes encoding aminoglycoside O-nucleotidyltransferases are widely spread among streptomycin resistant strains of Campylobacter jejuni and Campylobacter coli. Frontiers in Microbiology. 9:2515. 2018. (A). ISSN: 1664-302X. DOI: 10.3389/fmicb.2018.02515


SERVICIOS: