Inicio \ Investigación \ Publicaciones científicas \


A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus

BMC Veterinary Research publica este artículo de investigación

14 de agosto de 2015

Elephants are classified as critically endangered animals by the International Union for Conservation of Species (IUCN). Elephant endotheliotropic herpesvirus (EEHV) poses a large threat to breeding programs of captive Asian elephants by causing fatal haemorrhagic disease. EEHV infection is detected by PCR in samples from both clinically ill and asymptomatic elephants with an active infection, whereas latent carriers can be distinguished exclusively via serological assays. To date, identification of latent carriers has been challenging, since there are no serological assays capable of detecting seropositive elephants.

Here we describe a novel ELISA that specifically detects EEHV antibodies circulating in Asian elephant plasma/serum. Approximately 80 % of PCR positive elephants display EEHV-specific antibodies. Monitoring three Asian elephant herds from European zoos revealed that the serostatus of elephants within a herd varied from non-detectable to high titers. The antibody titers showed typical herpes-like rise-and-fall patterns in time which occur in all seropositive animals in the herd more or less simultaneously.

This study shows that the developed ELISA is suitable to detect antibodies specific to EEHV. It allows study of EEHV seroprevalence in Asian elephants. Results confirm that EEHV prevalence among Asian elephants (whether captive-born or wild-caught) is high




van del Doel PB., Rodriguez-Prieto V., van Rossum-Fikkert SE., Schaftenaar W., Latimer E., Howard L., Chapman S., Masters N., Osterhaus AD., Linq PD., Dastjerdi A. y Martina B.




Ver artículo
A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus

Ver en NLM PubMed
A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus


Participantes:

Erasmus Medical CenterViroScience Lab.. Erasmus Medical Center (Erasmus MC).

Artemis One Health Research Institute.

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Erasmus Medical CenterDepartment of Genetics. Erasmus Medical Center (Erasmus MC).

Rotterdam ZooVeterinary services. Rotterdam Zoo.

Smithsonian Conservation Biology Institute. Smithsonian’s National Zoo.

Department of Animal Health. Houston Zoo.

East-Midland Zoological Society. Twycross Zoo.

Zoological Society of London. Institute of Zoology (ZSL).

Department of Molecular Virology and Microbiology. Baylor College of Medicine (BCM).

Animal and Plant Health AgencyAnimal and Plant Health Agency (APHA).







BMC Veterinary Research
FACTOR YEAR Q
1.643 2015

NLMID: 101249759

PMID: 26268467

ISSN: 1746-6148



TÍTULO: A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus


REVISTA: BMC Vet. Res


NUMERACIÓN: 11:203


AÑO: 2015


EDITORIAL: BioMed Central


AUTORES: van del Doel PB., Rodriguez-Prieto V., van Rossum-Fikkert SE., Schaftenaar W., Latimer E., Howard L., Chapman S., Masters N., Osterhaus AD., Linq PD., Dastjerdi A. and Martina B.


DOI: https://doi.org/10.1186/s12917-015-0522-6


CITA ESTA PUBLICACIÓN:

van del Doel PB., Rodriguez-Prieto V., van Rossum-Fikkert SE., Schaftenaar W., Latimer E., Howard L., Chapman S., Masters N., Osterhaus AD., Linq PD., Dastjerdi A. y Martina B. A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus. BMC Veterinary Research. 11:203. 2015. (A). ISSN: 1746-6148. DOI: 10.1186/s12917-015-0522-6


SERVICIOS: