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How does temperature influences the development of lactococcosis? Transcriptomic and immunoproteomic in vitro approaches


1 de octubre de 2017

Investigación publicada en Journal of Fish Diseases


Lactococcus garvieae is the aetiological agent of lactococcosis, a haemorrhagic septicaemia that affects marine and freshwater fish, with special incidence and economic relevance in farmed rainbow trout. Water temperature is one of the most important predisposing factors in the development of lactococcosis outbreaks. Lactococcosis in trout usually occur when water temperatures rise to about 18 °C, while fish carriers remain asymptomatic at temperatures below 13 °C. The aim of this work was to analyse the differences in the complete transcriptome response of L. garvieae grown at 18 °C and at 13 °C and to identify the immunogenic proteins expressed by this bacterium at 18 °C. Our results show that water temperature influences the expression of L. garvieae genes involved in the lysis of part of the bacterial cell population and in the cold response bacterial adaptation. Moreover, the surface immunogenic protein profile at 18 °C suggests an important role of the lysozyme-like enzyme, WxL surface proteins and some putative moonlighting proteins (proteins with more than one function, usually associated with different cellular locations) as virulence factors in L. garvieae. The results of this study could provide insights into the understanding of the virulence mechanisms of L. garvieae in fish




Castro R., Reguera-Brito M., Lopez-Campos GH., Blanco MM., Aguado-Urda M., Fernandez-Garayzabal JF. y Gibello A.




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How does temperature influences the development of lactococcosis? Transcriptomic and immunoproteomic in vitro approaches

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How does temperature influences the development of lactococcosis? Transcriptomic and immunoproteomic in vitro approaches

Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

University of MelbourneHealth & Biomedical Informatics Research Unit. Medical School. University of Melbourne.

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Journal of Fish Diseases
FACTOR YEAR Q
2.138 2016

NLMID: 9881188

PMID: 28093775

ISSN: 0140-7775



TÍTULO: How does temperature influences the development of lactococcosis? Transcriptomic and immunoproteomic in vitro approaches


REVISTA: J. Fish Dis.


NUMERACIÓN: 40(10):1285-1297


AÑO: 2017


EDITORIAL: Blackwell Scientific Publications


AUTORES: Castro R., Reguera-Brito M., Lopez-Campos GH., Blanco MM., Aguado-Urda M., Fernandez-Garayzabal JF. and Gibello A.


PARTICIPANTES VISAVET


José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.1111/jfd.12601


CITA ESTA PUBLICACIÓN:

Castro R., Reguera-Brito M., Lopez-Campos GH., Blanco MM., Aguado-Urda M., Fernandez-Garayzabal JF. y Gibello A. How does temperature influences the development of lactococcosis? Transcriptomic and immunoproteomic in vitro approaches. Journal of Fish Diseases. 40(10):1285-1297. 2017. (A). ISSN: 0140-7775. DOI: 10.1111/jfd.12601


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