Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis
Investigación publicada en Clinical Proteomics
2 de noviembre de 2017
Bovine purified protein derivative (bPPD) and avian purified protein derivative (aPPD) are widely used for bovine tuberculosis diagnosis. However, little is known about their qualitative and quantitative characteristics, which makes their standardisation difficult. In addition, bPPD can give false-positive tuberculosis results because of sequence homology between Mycobacterium bovis (M. bovis) and M. avium proteins. Thus, the objective of this study was to carry out a proteomic characterisation of bPPD, aPPD and an immunopurified subcomplex from bPPD called P22 in order to identify proteins contributing to cross-reactivity among these three products in tuberculosis diagnosis
Infantes-Lorenzo JA., Moreno I., Risalde MA., Roy A., Villar M., Romero B., Ibarrola N., de la Fuente J., Puentes E., de Juan L., Gortazar C., Bezos J., Dominguez L. y Dominguez M.
 | Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII). |
 | Servicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
 | Sanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM). |
 | MAEVA SERVET, S.L.. |
| Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
 | Unidad de Proteómica. Instituto de Biología Molecular y Celular del Cáncer. Universidad de Salamanca (USAL). |
 | CZ Veterinaria, S.A. (CZV). |
 | Departament of Veterinary Pathobiology. Center for Veterinary Health Sciences (CVHS). Oklahoma State University (OSU). |
