Inicio \ Investigación \ Publicaciones científicas \


Phylogenomic analysis of the complete sequence of a gastroenteritis-associated cetacean adenovirus (bottlenose dolphin adenovirus 1) reveals a high degree of genetic divergence


12 de mayo de 2017

Investigación publicada en Infection Genetics and Evolution


Adenoviruses are common pathogens in vertebrates, infecting a wide range of hosts, but only having rarely been detected and correlated with disease in cetaceans. This article describes the first complete genomic sequence of a cetacean adenovirus, bottlenose dolphin adenovirus 1 (BdAdV-1), detected in captive bottlenose dolphin population (Tursiops truncatus) suffering from self-limiting gastroenteritis. The complete genome sequence of BdAdV-1 was recovered from data generated by high-throughput sequencing and validated by Sanger sequencing. The genome is 34,080bp long and has 220 nucleotides long inverted terminal repeats. A total of 29 coding sequences were identified, 26 of which were functionally annotated. Among the unusual features of this genome is a remarkably long 4380bp E3 ORF1, that displays no sequence homology with the corresponding E3 regions of other adenoviruses. In addition, the fiber protein only has 26% identity with fiber proteins described in other adenoviruses. Three hypothetical proteins were predicted. The phylogenetic analysis indicates that the closest known relative to BdAdV-1 is an adenovirus detected in bottlenose dolphin (KR024710), with an amino acid sequence identity between 36 and 79% depending on the protein. Based on the phylogenic analysis, the BdAdV-1 appears to have co-evolved with its host. The results indicate that BdAdV-1 belongs to the Mastadenovirus genus of the Adenoviridae family, however, it is clearly different from other adenoviruses, especially in the 3`-end of the viral genome. The high degree of sequence divergence suggests that BdAdV-1 should be considered as a novel species in the Mastadenovirus genus. The study also demonstrates the usefulness of high-throughput sequencing to obtain full-length genomes of genetically divergent viruses




Malmberg M., Rubio-Guerri C., Hayer J., Garcia-Parraga D., Nieto-Pelegrin E., Melero M., Alvaro T., Valls M., Sanchez-Vizcaino JM., Belak S. y Granberg F.




Ver artículo
Phylogenomic analysis of the complete sequence of a gastroenteritis-associated cetacean adenovirus (bottlenose dolphin adenovirus 1) reveals a high degree of genetic divergence

Ver en NLM PubMed
Phylogenomic analysis of the complete sequence of a gastroenteritis-associated cetacean adenovirus (bottlenose dolphin adenovirus 1) reveals a high degree of genetic divergence

Participantes:

Section of Virology. Department of Biomedical Sciences and Veterinary Public Health. Swedish University of Agricultural Sciences (SLU).

SLU Global Bioinformatics Centre. Department of Animal Breeding and Genetics. Swedish University of Agricultural Sciences (SLU).

National Veterinary InstituteOIE Collaborating Centre Skriv ut - for Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine. National Veterinary Institute (SVA).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Generalitat ValencianaOceanogràfic. Ciudad de las Artes y las Ciencias. Generalitat Valenciana.

Generalitat ValencianaVeterinary Services. Avanqua Oceanogràfic-Ágora. Ciudad de las Artes y las Ciencias. Generalitat Valenciana.







Infection Genetics and Evolution
FACTOR YEAR Q
2.591 2015

NLMID: 101084138

PMID: 28506838

ISSN: 1567-1348



TÍTULO: Phylogenomic analysis of the complete sequence of a gastroenteritis-associated cetacean adenovirus (bottlenose dolphin adenovirus 1) reveals a high degree of genetic divergence


REVISTA: Infect Genet Evol


NUMERACIÓN: S1567-1348(17)30159-4


AÑO: 2017


EDITORIAL: Elsevier Science


AUTORES: Malmberg M., Rubio-Guerri C., Hayer J., Garcia-Parraga D., Nieto-Pelegrin E., Melero M., Alvaro T., Valls M., Sanchez-Vizcaino JM., Belak S. and Granberg F.


PARTICIPANTES VISAVET


6th
Mar Melero Asensio
9th
José Manuel Sánchez-Vizcaíno Rodríguez

DOI: http://dx.doi.org/10.1016/j.meegid.2017.05.008


CITA ESTA PUBLICACIÓN:

Malmberg M., Rubio-Guerri C., Hayer J., Garcia-Parraga D., Nieto-Pelegrin E., Melero M., Alvaro T., Valls M., Sanchez-Vizcaino JM., Belak S. y Granberg F. Phylogenomic analysis of the complete sequence of a gastroenteritis-associated cetacean adenovirus (bottlenose dolphin adenovirus 1) reveals a high degree of genetic divergence. Infection Genetics and Evolution. S1567-1348(17)30159-4. 2017. (A). ISSN: 1567-1348. DOI: 10.1016/j.meegid.2017.05.008


SERVICIOS: