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Serosurvey Reveals Exposure to West Nile Virus in Asymptomatic Horse Populations in Central Spain Prior to Recent Disease Foci


1 de octubre de 2017

Artículo de investigación publicado en Transboundary and Emerging Diseases


West Nile fever/encephalitis (WNF) is an infectious disease affecting horses, birds and humans, with a cycle involving birds as natural reservoirs and mosquitoes as transmission vectors. It is a notifiable disease, re-emerging in Europe. In Spain, it first appeared in horses in the south (Andalusia) in 2010, where outbreaks occur every year since. However, in 2014, an outbreak was declared in horses in central Spain, approximately 200 km away from the closest foci in Andalusia. Before that, evidence of West Nile virus (WNV) circulation in central Spain had been obtained only from wildlife, but never in horses. The purpose of this work was to perform a serosurvey to retrospectively detect West Nile virus infections in asymptomatic horses in central Spain from 2011 to 2013, that is before the occurrence of the first outbreaks in the area. For that, serum samples from 369 horses, collected between September 2011 and November 2013 in central Spain, were analysed by ELISA (blocking and IgM) and confirmed by virus neutralization, proving its specificity using parallel titration with another flavivirus (Usutu virus). As a result, 10 of 369 horse serum samples analysed gave positive results by competitive ELISA, 5 of which were confirmed as positive to WNV by virus neutralization (seropositivity rate: 1.35%). One of these WNV seropositive samples was IgM-positive. Chronologically, the first positive samples, including the IgM-positive, corresponded to sera collected in 2012 in Madrid province. From these results, we concluded that WNV circulated in asymptomatic equine populations of central Spain at least since 2012, before the first disease outbreak reported in this area




Abad-Cobo A., Llorente F., Barbero MD., Cruz F., Fores P. y Jimenez-Clavero MA.




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Serosurvey Reveals Exposure to West Nile Virus in Asymptomatic Horse Populations in Central Spain Prior to Recent Disease Foci

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Serosurvey Reveals Exposure to West Nile Virus in Asymptomatic Horse Populations in Central Spain Prior to Recent Disease Foci

Participantes:

Instituto Nacional de Investigación y Tecnología Agraria y AlimentariaCentro de Investigación en Sanidad Animal (CISA). Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA).

Universidad ComplutenseServicio de Vigilancia Sanitaria Equina (SEVISEQ). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Medicina y Cirugía Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto de Salud Carlos IIICentro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP). Instituto de Salud Carlos III (ISCIII).







Transboundary and Emerging Diseases
FACTOR YEAR Q
3.585 2016

NLMID: 101319538

PMID: 27156847

ISSN: 1865-1674



TÍTULO: Serosurvey Reveals Exposure to West Nile Virus in Asymptomatic Horse Populations in Central Spain Prior to Recent Disease Foci


REVISTA: Transbound. Emerg. Dis.


NUMERACIÓN: 64(5):1387-1392


AÑO: 2017


EDITORIAL: Blackwell Verlag


AUTORES: Abad-Cobo A., Llorente F., Barbero MD., Cruz F., Fores P. and Jimenez-Clavero MA.


PARTICIPANTES VISAVET


Fátima Cruz López

DOI: https://doi.org/10.1111/tbed.12510


CITA ESTA PUBLICACIÓN:

Abad-Cobo A., Llorente F., Barbero MD., Cruz F., Fores P. y Jimenez-Clavero MA. Serosurvey Reveals Exposure to West Nile Virus in Asymptomatic Horse Populations in Central Spain Prior to Recent Disease Foci. Transboundary and Emerging Diseases. 64(5):1387-1392. 2017. (A). ISSN: 1865-1674. DOI: 10.1111/tbed.12510


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