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Evaluation of five serologic assays for bovine tuberculosis surveillance in domestic free-range pigs from southern Spain


1 de febrero de 2017

Preventive Veterinary Medicine publica este artículo de investigación


In countries where bovine tuberculosis (bTB) is still prevalent the contact among different animal species in extensive systems contributes to the circulation of Mycobacterium bovis (M. bovis) and other members of the Mycobacterium tuberculosis complex (MTC). Thus, free-range pigs can develop subclinical infections and may contribute to disease spread to bovine and wildlife. Serodiagnosis has been proposed as a screening tool for detecting infected pig herds; however, the value of this method to obtain an accurate diagnosis in this species is still not clear. In this study, sensitivity (Se) and specificity (Sp) estimates of four ELISAs and a lateral flow immunochromatographic antibody assay based on different M. bovis antigens, including MPB70 and MPB83 proteins, were evaluated in naturally infected domestic free-range pigs. For this purpose, submandibular lymph nodes and blood samples from 217 pigs from both TB-infected and historically negative farms were sampled at slaughterhouse and analysed by gross examination, histopathology, bacteriological culture and qPCR. Se and Sp estimates of the 5 evaluated assays ranged from 66.1% to 78% (CI95 from 52.6 to 87.7%) and from 98.9% to 100% (CI95 from 93.8 to 100%), respectively. Results of our study suggest that all the evaluated assays could be used as a first screening tool to conduct bTB surveillance in domestic pigs at population level; however, animals from seropositive herds should later be surveyed by other methods in order to reduce false negative results




Cardoso-Toset F., Luque I., Carrasco L., Jurado-Martos F., Risalde MA., Venteo A., Infantes-Lorenzo JA., Bezos J., Rueda P., Tapia I., Gortazar C., Dominguez L., Dominguez M. y Gomez-Laguna J.




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Evaluation of five serologic assays for bovine tuberculosis surveillance in domestic free-range pigs from southern Spain

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Evaluation of five serologic assays for bovine tuberculosis surveillance in domestic free-range pigs from southern Spain

Participantes:

Centro de Investigación y Calidad AgroalimentariaCentro de Investigación y Calidad Agroalimentaria (CICAP).

Universidad de CórdobaDepartamento de Anatomía y Anatomía Patológica Comparadas. Facultad de Veterinaria. Universidad de Córdoba (UCO).

Gobierno de Castilla-La ManchaInstituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Inmunología y Genética Aplicada, S.A.Inmunología y Genética Aplicada, S.A. (INGENASA).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).







Preventive Veterinary Medicine
FACTOR YEAR Q
2.182 2015

NLMID: 8217463

PMID: 28089289

ISSN: 0167-5877



TÍTULO: Evaluation of five serologic assays for bovine tuberculosis surveillance in domestic free-range pigs from southern Spain


REVISTA: Prev. Vet. Med.


NUMERACIÓN: 137(2017):101-104


AÑO: 2017


EDITORIAL: Elsevier Science Publishers


AUTORES: Cardoso-Toset F., Luque I., Carrasco L., Jurado-Martos F., Risalde MA., Venteo A., Infantes-Lorenzo JA., Bezos J., Rueda P., Tapia I., Gortazar C., Dominguez L., Dominguez M. and Gomez-Laguna J.


PARTICIPANTES VISAVET


7th
Jose Antonio Infantes Lorenzo
8th
Javier Bezos Garrido
12th
Lucas Domínguez Rodríguez

DOI: http://dx.doi.org/10.1016/j.prevetmed.2016.12.016


CITA ESTA PUBLICACIÓN:

Cardoso-Toset F., Luque I., Carrasco L., Jurado-Martos F., Risalde MA., Venteo A., Infantes-Lorenzo JA., Bezos J., Rueda P., Tapia I., Gortazar C., Dominguez L., Dominguez M. y Gomez-Laguna J. Evaluation of five serologic assays for bovine tuberculosis surveillance in domestic free-range pigs from southern Spain. Preventive Veterinary Medicine. 137(2017):101-104. 2017. (A). ISSN: 0167-5877. DOI: 10.1016/j.prevetmed.2016.12.016


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