Publicaciones científicas SCI 2026
A dual-gene-deleted ASFV Lv17/WB/Rie1-?CD candidate administered orally to wild boar confers DIVA-compatible protection against virulent challenge
The Veterinary Quarterly 46(1):2649573.
African swine fever (ASF) continues to expand worldwide. Recent detection in wild boar in Spain highlights the urgent need for effective control tools, with oral vaccination as a key priority. Following previous evaluation of the attenuated Lv17/WB/Rie1 strain, we assess an improved derivative, Lv17/WB/Rie1-?CD, lacking EP402R (CD2v) and EP153R, replaced by GFP to abrogate haemadsorption and enable Differentiating-Infected-from-Vaccinated-Animals (DIVA) diagnostics. Vaccinated animals received either a single high dose (104 TCID??) or a prime and re-exposure regimen (102 TCID?? plus a 104 TCID??). Animals were challenged intramuscularly with the virulent Armenia07 genotype II strain. The ?CD vaccine was well tolerated, inducing only transie… Leer más
Barasona JA., Kosowska A., Gonzalez-Garcia G., Diaz-de Frutos M., Franzoni G., Nicolussi P., Porras N., Sanchez-Segovia M., de Antonio Gomez DE., Rueda P. y Barroso-Arevalo S.
Hepatitis E virus in pigs at the moment of slaughter in Spain, 2015 and 2017
Food Microbiology 136:105011.
We investigated the presence of hepatitis E virus (HEV) in pigs at the moment of slaughter in Spain in years 2015 and 2017. A total of 1786 caecal content, liver, and serum samples from animals at slaughterhouses were tested by reverse transcription real-time quantitative PCR (RT-qPCR) and anti-HEV antibodies were evaluated in 623 serum samples by an ELISA test. The overall seroprevalence obtained was 70.9 %. A total of 398 RT-qPCR positive samples were identified in caecal content (26.8 %; 156/583), serum (21.8 %; 136/623) and liver (18.3 %; 106/580). A total of 32 RT-qPCR positive samples were genotyped; 3f (84.4 %) and the 3c (9.4 %) being the most prevalent subgenotypes. This is the first report on detection of HEV in pigs at the moment… Leer más
Garcia N., Hernandez M., Santamaria-Palacios J., Martinez I., Navarro A., Munoz-Chimeno M., Escobar F., Fongaro G., Yeramian N., Trzakowska M., Avellon A., Eiros JM., Dominguez L., Valero A., Goyache J. y Rodriguez-Lazaro D.
Evaluation of four molecular detection assays for the diagnosis of Chlamydia pecorum infection in Victorian koalas (Phascolarctos cinereus) using Bayesian latent class analysis
Preventive Veterinary Medicine 250:106809.
Chlamydiosis poses a significant threat to koala health and population viability. Accurate diagnosis of chlamydial infection in koalas at veterinary hospitals informs patient prognosis, biosecurity and determines treatment. However, there is no standardised approach to the diagnosis of chlamydiosis in wildlife hospitals. The performance of available diagnostic tests has not previously been rigorously assessed, and there is a lack of sufficient well-characterised reference samples. This study aimed to estimate the diagnostic accuracy of four molecular detection assays for Chlamydia pecorum infection in koalas using Bayesian latent class analysis (BLCA). Swabs were collected from koalas presented to a wildlife hospital (n?=?45) and historical… Leer más
Wheelahan JW., Gómez-Buendía A., Legione AR., Firestone SM., Vaz PK., Hartley CA., Rourke NL. y Devlin JM.
Estimating the velocity and direction of African Swine Fever spread in wild boar populations in South Korea using Trend-Surface Analysis
PLoS ONE 21(4):e0346098.
African swine fever (ASF) is a lethal disease of swine that has spread across Asia since its introduction in 2018. South Korea first reported the disease in September 2019 in domestic pigs, and since then, more than 4,000 cases have been reported in wild boars during its expansion up to August 2024. Due to the high number of ASF notifications in wild boars in South Korea, contrasted with their scarcity in most Asian countries, analyzing the spatiotemporal spread of the disease in a setting with active surveillance provides valuable insights. In this study, we performed a trend-surface analysis on temporally gridded case data to characterize the overall geographic spread and direction of ASF in wild boars across South Korea, from its emergen… Leer más
Aguilar-Vega C., Bosch J., Ito S., Ivorra B., Jeong H. y Sanchez-Vizcaino JM.
Development of an in situ hybridization assay for the diagnosis of Mycobacteriaceae infections of veterinary importance
Veterinary Pathology 63(2):223-232.
Mycobacteria (Mycobacteriaceae family) comprise five genera (Mycobacterium, Mycolicibacterium, Mycolicibacter, Mycolicibacillus, and Mycobacteroides), which include relevant animal and human pathogens. Histology is a rapid method for preemptively diagnosing mycobacteriosis, contributing to surveillance, control, and eradication. A constraint on histology is the limited sensitivity and specificity of acid-fast stains, as the number of detectable bacilli in formalin-fixed paraffin-embedded (FFPE) tissue varies and other microorganisms are acid-fast positive. Immunohistochemistry has low specificity and is cross-reactive with other bacteria. We developed an RNAscope probe-based in situ hybridization (ISH) assay, targeting a conserved sequence … Leer más
Rebollada A., McDonough SP., Uzal FA., Rodriguez-Bertos A., Getchell RG., Nakagun S. y Demeter EA.
Histological and Immunohistochemical Characterization of a Histiocytic Sarcoma Associated With a Follicular Lymphoma in a Ring-tailed Lemur (Lemur catta)
Veterinary Medicine And Science 12(2):e70800.
A 20-year-old neutered male ring-tailed lemur (Lemur catta) diagnosed with an intrathoracic mass died during surgical excision. Morphological characteristics of the mass during necropsy were consistent with a mediastinal abscess in the left hemithorax. Histological examination identified a disseminated histiocytic sarcoma involving spleen, kidneys, multiple lymph nodes, diaphragm and parietal pleura, as well as a concurrent follicular lymphoma in a separate lymph node. A mixed metastatic infiltrate composed of both neoplasms was found within the capsule of the intrathoracic abscess. Immunohistochemical analysis (Iba-1, CD3, PAX-5, IRF-4, pancytokeratin AE1/AE3 and Ki-67) confirmed both neoplasms and revealed mild IRF-4 immunoreactivity for … Leer más
Aradilla-Macias N., Canales R., Lorenzo-Bermejo T., Mainez M., Aguiló-Gisbert J., Marbella D., De Pablo-Moreno J. y Rodriguez-Bertos A..
Exploring specific biomarkers in blood for in vitro diagnosis of caprine tuberculosis
Frontiers In Microbiology 17:1765857.
Introduction: Caprine tuberculosis (cTB) is a zoonotic disease with significant implications for animal and public health and economic impact. Therefore, accurate ante-mortem diagnosis is essential. Diagnosis in goats rely on the single and comparative intradermal tuberculin test (SITT and CITT, respectively), both of which showing limited diagnostic performance. The aim of the study was to
characterize the immune and physiological responses to cTB in naturally infected goats and to identify potential in vitro biomarkers that could improve the reliability of cTB diagnosis.
Methodology: The study was conducted in a cTB infected herd and goats were classified as reactors (positive to SITT or IFN-? release assay and considered as… Leer más
Velasco-Reinaldos C., Roy A., Cruz F., Gómez-Buendía A., Ortega J., Mendez-Lopez S., de Juan L., Dominguez L., Romero B., Alvarez J. y Bezos J..
Editorial: One Health approach to mycobacterial infections in veterinary science
Frontiers In Veterinary Science 13:1778469.
Mycobacteria are ubiquitous pathogens that affect livestock, companion animals, wildlife, and humans, posing major challenges for veterinary medicine. Accurate diagnosis and targeted treatment are crucial to prevent antimicrobial resistance, yet integrated actions across the human–animal–environment interface remain limited, highlighting the need for a One Health approach (1, 2).
This Research Topic compiles 11 articles that advance the One Health understanding of veterinary-relevant mycobacteria by examining their epidemiology, diagnosis, treatment, zoonotic transmission dynamics, and by proposing integrated strategies to strengthen surveillance and disease control across interconnected systems.
Epidemiolo… Leer más
Marfil MJ., Traversa MJ., Barandiaran S., Ferrara-Muniz X., Zumarraga MJ. y Eirin ME.
Ineffective oral immunization of wild boar with the attenuated African swine fever virus NH/P68 grown in MA104 cell line
The Veterinary Journal 315:106554.
African swine fever (ASF) is a highly contagious and lethal hemorrhagic disease affecting domestic pigs and wild boar, with serious consequences for animal health, the swine industry, and socio-economic stability. Given the lack of effective treatments and the limited success of conventional control measures, live attenuated vaccines (LAVs) have emerged as the most promising option, particularly for oral administration in free-ranging wild boar. The naturally attenuated genotype I isolate NH/P68 has shown protective potential in domestic pigs but retains residual virulence and persistence risks. To increase attenuation and facilitate large-scale production, the isolate was adapted to the MA104 continuous cell line. In this study, we assesse… Leer más
Kosowska A., Sanchez-Segovia M., Fernandez-Pinero J., Porras N., Barroso-Arevalo S., Sánchez-Morales L., Diaz-de Frutos M. y Barasona JA..
Editorial: Pathogen transmission at the domestic-wildlife interface: a growing challenge that requires integrated solutions, volume II
Frontiers In Veterinary Science 13:1780843.
Domestic-wildlife interfaces constitute dynamic and heterogeneous systems in which ecological, epidemiological, and socio-economic processes converge, creating opportunities for pathogen transmission between wildlife, domestic animals, humans, and the shared environment. These interfaces evolve in response to several factors including land-use change, agricultural intensification, wildlife population recovery, animal trade, globalization, or human mobility, among others, all of which reshape pathogen transmission pathways across spatial and temporal scales (1, 2).
Volume I emphasized the need for integrative and interdisciplinary research to characterize eco-epidemiological drivers in these complex epidemiological systems to inform d… Leer más
Jiménez-Ruiz S., Jori F., Santos N., Barasona JA. y Fine AE.
Molecular Detection of Theileria equi, Babesia caballi, and Borrelia burgdorferi Sensu Lato in Hippobosca equina from Horses in Spain
Pathogens 15(1):94.
The forest fly (Hippobosca equina) is an obligate haematophagous dipteran insect (order Diptera) that primarily infests horses and may contribute to the circulation of vector-borne pathogens. This study aimed to investigate the presence of Anaplasma phagocytophilum, Borrelia burgdorferi s.l., Babesia caballi, and Theileria equi, important vector-borne pathogens of equids, in forest flies collected from horses in endemic areas of Spain. A total of 170 forest flies were collected from 39 equids across four geographical regions in Spain (Segovia, Madrid, Toledo, and Menorca) and blood samples were collected from 27 of these horses. All flies were morphologically and molecularly identified as H. equina, and DNA extracted from flies and equine b… Leer más
Dorrego-Rodriguez A., Olvera-Maneu S., Jose-Cunilleras E., Gago-Munoz P., Raez-Casarrubios A., Rivera B., Oporto-Paita A., Gonzalez S. y Cruz F..
Towards Integrated Surveillance of Marine Brucellosis: Diagnostic and Phylogenetic Assessment of Brucella ceti in Stranded Dolphins of the Western Mediterranean Sea
Transboundary And Emerging Diseases 31:2026:2075116.
Reports of brucellosis in free-ranging cetaceans are increasing worldwide, particularly in the Mediterranean Sea. To enhance diagnostic accuracy and epidemiological understanding of cetacean brucellosis in the Western Mediterranean Sea, we analyzed bacteriological, serological, and molecular data from 30 cetaceans belonging to three different species stranded along the coast of the Valencian Community (Spain) between 2011 and 2021. Brucella ceti infection was confirmed by bacteriological isolation in 14 animals (46.7%) and by genus-specific qPCR in 15 cases (50%), with some discrepancies between methods. When feasible, serological analyses were performed using a commercial blocking ELISA (bELISA) and/or the Rose Bengal agglutination test (R… Leer más
Vargas-Castro I., Andres-Barranco S., Crespo-Picazo JL., Torre-Fuentes L., Jimenez-Martinez MA., Hernandez M., Arbelo M., Alvarez J., Munoz PM., Marco-Cabedo V., de Miguel MJ., López-Gutiérrez D., Munoz-Baquero M., Garcia-Parraga D. y Barasona JA..
Pathological and microbiological assessment of hemorrhagic septicemia in cultured juvenile Russian sturgeons (Acipenser gueldenstaedtii)
Aquaculture Reports 46:10334600.
Aquaculture has experienced sustained growth, now surpassing capture fisheries in global production. Among cultured species, sturgeon aquaculture has likewise expanded recently, prompting increased research and surveillance of diseases affecting this taxon. In this context, fifteen farmed Russian sturgeons (Acipenser gueldenstaedtii) were submitted for pathological investigation. A comprehensive study was conducted, encompassing autopsy, histopathology, parasitology, microbiology, and antimicrobial resistance analysis. Macroscopic examination revealed ulcerative lesions on the scutes, multiorgan hemorrhages, and multifocal black lesions in the liver. Histological analysis revealed a systemic granulocytic inflammatory response with associate… Leer más
De Pablo-Moreno J., Chinchilla-Rodríguez B., Blazquez JA., Aradilla-Macias N., Santos-Lopez S., Garcia-Saorin AJ. y Rodriguez-Bertos A..
Evaluation of trained immunity as a potential strategy for a universal Streptococcus suis vaccine using immunogenic proteins in a murine model
BMC Veterinary Research 22(1):66.
Background: Universal vaccine against Streptococcus suis (S. suis) remains a challenge due to the high number of serotypes/strains, the absence of cross-protection among them and the existence of different immune escape strategies. In this study, the immunomodulatory potential of live and inactivated porcine derived Bacillus Calmette-Guérin (dpB) in combination with specific immunogenic proteins S. suis is evaluated in murine model.
Results: Results revealed different immune responses depending on dpB formulation. Live-dpB administered intravenously induced consistent results with trained immunity, including elevated proinflammatory cytokines and enhanced phagocytosis activity, reflecting stronger innate immune activation. However, … Leer más
Sánchez-Morales L., Zamora L., Perez-Sancho M., Garcia-Seco T., Moreno I., Perez-Domingo A., Diaz-de Frutos M., Ciordia S., Buendia A., Risalde MA., Dominguez M. y Dominguez L..
Abattoir Surveillance: Identifying Risk Factors Associated with Bovine Tuberculosis Lesion Detection in a Low Prevalence Region
Preventive Veterinary Medicine 246:106710 .
Abattoir surveillance is a key component in control and eradication programs against bovine tuberculosis (bTB). In low-prevalence or officially tuberculosis-free (OTF) regions in which active surveillance is typically limited or non-existent, postmortem inspection at the abattoir constitutes one of the main diagnostic tool to guarantee the absence of disease transmission. Here, we evaluated the performance of abattoir postmortem inspection in Galicia, a low-prevalence region in Spain (now OTF). Between 2014 and 2019, 1,784,261 animals were culled in 41 abattoirs, of which a small proportion (0.74%, n=13,200) were reactors in bTB antemortem tests. Two mixed-effects logistic regression models assessed the risk of detecting bTB-compatible lesi… Leer más
Gómez-Buendía A., Triguero-Ocana R., Pozo P., Munoz-Mendoza M., Alvarez-Fidalgo M., Mourelo J., Romero B. y Alvarez J..
