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Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis

Veterinary Research publish this investigation article

July 1st, 2006

The intradermal tuberculin (IDTB) test and the interferon-gamma (IFN-gamma) assay are used worldwide for detection of bovine tuberculosis in cattle, but little is known about the effect of co-infecting agents on the performance of these diagnostic tests. This report describes a field trial conducted in a cattle herd with dual infection (bovine tuberculosis and paratuberculosis) during 3.5 years. It has been based on a strategic approach encompassing serial parallel testing (comparative IDTB test, the IFN-gamma assay and serology of paratuberculosis) that was repeated 8 times over the period, and segregation of animals into two herds. The IDTB test detected 65.2% and the IFN-gamma test detected 69.6% of the Mycobacterium bovis culture-positive cattle. However, the IDTB test performed better during the first part of the trial, while the IFN-gamma test was the only method that detected infected animals during the following three samplings. The number of false positive reactors with the IDTB and/or the IFN-gamma tests was remarkably high compared to other reports, and could be caused by cross-reactivity with M. avium subsp. paratuberculosis. Also, the M. bovis isolates from cattle and wildlife from the same property were characterised using molecular techniques to disclose an epidemiological link. The IDTB test may not be appropriate to eradicate bovine tuberculosis in herds with dual mycobacterial infections. This report highlights the need to use several diagnostic techniques for the accurate detection of M. bovis infected animals in these herds




Aranaz A., de Juan L., Bezos J., Alvarez J., Romero B., Lozano F., Paramio JL., Lopez-Sanchez J., Mateos A. and Dominguez L..




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Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis

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Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis


Participants:

Ministerio de Agricultura, Pesca y AlimentaciónMinisterio de Agricultura, Pesca y Alimentación (MAPA).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Laboratorio Regional Pecuario. Consejería de Agricultura y Desarrollo Rural.

Gobierno de Castilla-La ManchaLaboratorio Regional Pecuario. Gobierno de Castilla-La Mancha (JCCM).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Veterinary Research
FACTOR YEAR Q
3.152 2006

NLMID: 9309551

PMID: 16701065

ISSN: 0928-4249



TITLE: Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis


JOURNAL: Vet. Res.


NUMERACIÓN: 37(4):593-606


AÑO: 2006


PUBLISHER: BMC


AUTHORS: Aranaz A., de Juan L., Bezos J., Alvarez J., Romero B., Lozano F., Paramio JL., Lopez-Sanchez J., Mateos A. and Dominguez L..


VISAVET PARTICIPANTS


2nd
Lucía de Juan Ferré
3rd
Javier Bezos Garrido
4th
Julio Álvarez Sánchez
5th
Beatriz Romero Martínez
6th
Francisco Javier Lozano Barrilero
Last
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1051/vetres:2006021


CITE THIS PUBLICATION:

Aranaz A., de Juan L., Bezos J., Alvarez J., Romero B., Lozano F., Paramio JL., Lopez-Sanchez J., Mateos A. and Dominguez L. Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis. Veterinary Research. 37(4):593-606. 2006. (A). ISSN: 0928-4249. DOI: 10.1051/vetres:2006021


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