Pseudomonas simiae sp. nov., isolated from clinical specimens from monkeys (Callithrix geoffroyi)
International Journal of Systematic and Evolutionary Microbiology publish this investigation article
November 1st, 2006
An unusual Gram-negative, catalase- and oxidase-positive, rod-shaped bacterium isolated from different clinical samples from two monkeys (Callithrix geoffroyi) was characterized by phenotypic and molecular genetic methods. The micro-organism was tentatively identified as a Pseudomonas species on the basis of the results of cellular morphological and biochemical tests. Fatty acid studies confirmed this generic placement and comparative 16S rRNA gene sequencing studies demonstrated that the unknown isolates were phylogenetically closely related to each other (100% sequence similarity) and were part of the `Pseudomonas fluorescens intrageneric cluster`. The novel bacterium, however, was distinguished from other phylogenetically related species of Pseudomonas by DNA-DNA hybridization studies and biochemical tests. On the basis of both phenotypic and phylogenetic findings, it is proposed that the novel Pseudomonas isolates are classified as Pseudomonas simiae sp. nov. The type strain of P. simiae is OLiT (=CCUG 50988T=CECT 7078T)
Vela AI., Gutierrez M., Falsen E., Rollan E., Simarro I., Garcia P., Dominguez L., Ventosa A. and Fernandez-Garayzabal JF..
 | Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
| Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
 | Departamento de Microbiología y Parasitología. Facultad de Farmacia. Universidad de Sevilla (US). |
| Department of Clinical Bacteriology. University of Gothenburg. | |
| Departamento de Medicina y Cirugía Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
