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Short communication. First detection of Israeli Acute ParalysisVirus (IAPV) in Spanish honeybees

Investigation published in Spanish Journal of Agricultural Research

June 1st, 2010

Spanish bee samples were analyzed for the presence of Israel Acute Paralysis Virus (IAPV). Some of these samples were collected from colonies presenting compatible symptoms with the colony collapse disorder (CCD, 240 out of 484) and the rest were asymptomatic. Only one of these samples was diagnosed as positive to IAPV by employing a one step RT-PCR that targets the ORF 2 of the IAPV genome. Specificity of the RT-PCR assay was evaluated by sequence analysis of size specific amplification products. IAPV nucleotide sequences already published in GeneBank were used to construct a phylogenetic tree that included the new Spanish IAPV sequence (FJ821506). They segregated in two main lineages and the Spanish isolate was mainly related with the American ones. As IAPV was detected in Spain in a very low frequency, no causal relation between IAPV and CCD in Spain was found




Kukielka D. and Sanchez-Vizcaino JM..




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Short communication. First detection of Israeli Acute ParalysisVirus (IAPV) in Spanish honeybees





Participants:

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







FACTOR YEAR Q
0.646 2010

NLMID: 101189412

ISSN: 1695-971X



TITLE: Short communication. First detection of Israeli Acute ParalysisVirus (IAPV) in Spanish honeybees


JOURNAL: Span J Agric Res


NUMERACIÓN: 8(2):308-311


AÑO: 2010


PUBLISHER: Spanish National Institute for Agricultural and Food Research and Technology (INIA)


AUTHORS: Kukielka D. and Sanchez-Vizcaino JM..


José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/10.5424/sjar/2010082-1218


CITE THIS PUBLICATION:

Kukielka D. and Sanchez-Vizcaino JM. Short communication. First detection of Israeli Acute ParalysisVirus (IAPV) in Spanish honeybees. Spanish Journal of Agricultural Research. 8(2):308-311. 2010. (A). ISSN: 1695-971X. DOI: 10.5424/sjar/2010082-1218


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