Investigation published in Journal of AOAC International

January 1^st, 1994

An economical, fast, and simple method for the extraction and liquid chromatographic determination of chloramphenicol (CAP) in milk is described. CAP is extracted by using a recently developed membrane-based method named "diphasic dialysis." CAP is detected and quantitated in the organic solvent used in dialysis without additional cleanup steps by reversed-phase liquid chromatography and UV detection (270 nm). The determination limit of CAP in milk was about 5 micrograms/L, although as little as 1 microgram/L could be detected under optimal working conditions

Bayo FJ., Moreno MA., Prieta J., Diaz S., Suarez G. and Dominguez L..

Chloramphenicol extraction from milk by using the diphasic dialysis method followed by liquid chromatographic determination

	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Servicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).