Investigation published in FEMS Microbiology Letters

November 15^th, 1994

A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 x 10(2) to 2.5 x 10(4) transformants per 10(6) viable protoplasts and microgram DNA. The stability of the plasmids in the transformants was also studied. This transformation system offers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants

Moreno MA., Pascual C., Gibello A., Ferrer S., Bos CJ., Debets AJ. and Suarez G.

Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans

Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans

	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Servicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).