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Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B

Investigation published in Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health

January 1st, 1994

Three monoclonal antibodies (MAbs) which react specifically with staphylococcal enterotoxin B (SEB) were studied for their suitability for use in ELISA. One of them (MAb B14) worked well as a coating antibody; MAb B12 was shown to be a good probing antibody (conjugated with peroxidase) when ELISA plates were coated with MAb B14. This effective pair of MAbs (B14-B12PO) is able to detect 0.625 ng SEB ml, and to distinguish between SEB and other proteins present in food extracts




Goyache J., Orden JA., Blanco JL., Domenech A., Suarez G. and Gomez-Lucia E.




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Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B

See it on NLM PubMed
Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







FACTOR YEAR Q
0.662 1997

NLMID: 100955260

PMID: 7597856

ISSN: 0931-1793



TITLE: Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B


JOURNAL: J. Vet. Med. B Infect. Dis. Vet. Public Health


NUMERACIÓN: 41(10):639-44


AÑO: 1994


PUBLISHER: Blackwell Scientific Publications


AUTHORS: Goyache J., Orden JA., Blanco JL., Domenech A., Suarez G. and Gomez-Lucia E.


VISAVET PARTICIPANTS


First
Joaquín Goyache Goñi
5th
Guillermo Suárez Fernández

DOI: https://doi.org/ 10.1111/j.1439-0450.1994.tb00274.x


CITE THIS PUBLICATION:

Goyache J., Orden JA., Blanco JL., Domenech A., Suarez G. and Gomez-Lucia E. Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health. 41(10):639-44. 1994. (A). ISSN: 0931-1793. DOI: 10.1111/j.1439-0450.1994.tb00274.x


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