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Pathogenesis of lymphoid lesions in murine experimental listeriosis

Investigation article published in Journal of Comparative Pathology

July 1st, 1991

Adult female Swiss albino mice were infected intraperitoneally or subcutaneously with Listeria monocytogenes Serovar 4b or 1/2a and killed at intervals. Thymus, spleen, Peyer`s patches and a variety of lymph nodes, including the jejunal (mesenteric), mediastinal, lumbar, mandibular and superficial inguinal, were examined by histopathology and by immunocytochemistry for detection of L. monocytogenes antigen. Similar results were obtained with both Serovars and by both routes of inoculation used. In the spleen, L. monocytogenes was detected, by immunoperoxidase staining, as soon as 4 h after inoculation, inside phagocytic cells located predominantly in the marginal zone of the white pulp. This was followed by inflammation, necrosis and depletion of lymphoid cells, which extended in extreme cases to the whole organ. Inflammatory lesions diminished progressively at 5 to 6 days after inoculation. In animals dying of the infection, a severe necrotizing splenitis was present. Depletion of lymphoid cells and inflammatory changes were widespread in the lymph nodes and to a lesser extent in the Peyer`s patches. An extensive necrotizing lymphadenitis was the prominent lesion in severely affected nodes. Inflammatory lesions and detection of L. monocytogenes antigen started around the venules of high endothelium. A thymus depletion, not associated with the multiplication of bacteria in the organ, was also a constant feature of the infection. This study suggests that L. monocytogenes (1) is transported to the spleen and to the lymph nodes by phagocytes, entering the organs by the marginal sinus in the spleen and by the venules of high endothelium in the lymph nodes; (2) multiplies in these cells as well as in neutrophilic granulocytes (the latter rapidly migrate to the affected zones); and (3) induce a splenitis and lymphadenitis, involving predominantly T cell-dependent areas, with a necrotizing component in severe cases. From our observations it is concluded that infection of the lymphoid system is a major feature in the pathogenesis of murine listeriosis




Marco A., Domingo M., Prats M., Briones V., Pumarola M. and Dominguez L..




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Pathogenesis of lymphoid lesions in murine experimental listeriosis

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Pathogenesis of lymphoid lesions in murine experimental listeriosis



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Journal of Comparative Pathology
FACTOR YEAR Q
1.030 2005

NLMID: 102444

PMID: 1918447

ISSN: 0021-9975



TITLE: Pathogenesis of lymphoid lesions in murine experimental listeriosis


JOURNAL: J Comp Pathol


NUMERACIÓN: 105(1):1-15


AÑO: 1991


PUBLISHER: Academic Press


AUTHORS: Marco A., Domingo M., Prats M., Briones V., Pumarola M. and Dominguez L..


4th
Víctor Briones Dieste
Last
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/S0021-9975(08)80057-6


CITE THIS PUBLICATION:

Marco A., Domingo M., Prats M., Briones V., Pumarola M. and Dominguez L. Pathogenesis of lymphoid lesions in murine experimental listeriosis. Journal of Comparative Pathology. 105(1):1-15. 1991. (A). ISSN: 0021-9975. DOI: 10.1016/S0021-9975(08)80057-6


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