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August, 2013 news

August 31st, 2013

Via publiDB

Enfermedades Infecciosas y Microbiología Clínica

First report of colistin-resistant KPC-2 producing ST258-Klebsiella pneumoniae in Spain

Investigation published in Enfermedades Infecciosas y Microbiología Clínica

The rapid spread of Klebsiella pneumoniae carbapenemases (KPCs) in the last years represents a serious public health threat. KPCs have a great potential for spread due to their location on mobile genetic elements that are horizontally transferred and associated with widespread clones.1 Many KPC producing K. pneumoniae isolates are associated to a single sequence type (ST)258, a clonal lineage that has been causing epidemics of great proportions in various regions of USA, Israel, Greece and recently in other European countries.2, 3 Organisms carrying carbapenemases usually harbours resistance d… Read more

August 30th, 2013

Via publiDB

Veterinary Microbiology

Genetic analysis of Streptococcus suis isolates from wild rabbits

Veterinary Microbiology publish this investigation article

This work aims to investigate the presence of Streptococcus suis in wild rabbits. A total of 65 S. suis isolates were recovered from 33.3% of the wild rabbits examined. Most isolates (86.2%) belong to genotype cps9. These isolates were further characterized by pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and virulence genotyping. Overall, S. suis exhibited a low genetic diversity. Only 5 genetic profiles were obtained by PFGE and most isolates (71.4%) were included in two pulsotypes that were also widely distributed among the wild rabbit population. MLST analysis … Read more

August 28th, 2013

Via publiDB

Early detection of mortality in poultry production using high resolution thermography

Oral communication in VII Congreso Ibérico de Agroingeniería y Ciencias Hortícolas

Modern poultry production facilities grouped together a very high number of individuals in battery cages. This concentration represents a challenge for the temperature control inside the buildings, to ensure that animals are within their range of thermal comfort (12-24 ° C in the case of laying hens). Similarly, early detection of potential problems or dead individuals is an essential task to ensure the welfare of the remaining animals. Continuous monitoring of the temperature in poultry houses may contribute to the first mentioned aspect, while early detection of dead animals or those affecte… Read more

August 1st, 2013

Via publiDB

Indigenous and acquired modifications in the aminoglycoside binding sites of Pseudomonas aeruginosa rRNAs

Investigation published in RNA Biology

Aminoglycoside antibiotics remain the drugs of choice for treatment of Pseudomonas aeruginosa infections, particularly for respiratory complications in cystic-fibrosis patients. Previous studies on other bacteria have shown that aminoglycosides have their primary target within the decoding region of 16S rRNA helix 44 with a secondary target in 23S rRNA helix 69. Here, we have mapped P. aeruginosa rRNAs using MALDI mass spectrometry and reverse transcriptase primer extension to identify nucleotide modifications that could influence aminoglycoside interactions. Helices 44 and 45 contain indigeno… Read more

August 1st, 2013

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Transboundary and Emerging Diseases

Development of a suspension microarray for the genotyping of African Swine Fever Virus targeting the SNPs in the c-terminal end of the p72 gene region of the genome

Investigation published in Transboundary and Emerging Diseases

African swine fever virus (ASFV) causes one of the most dreaded transboundary animal diseases (TADs) in Suidae. African swine fever (ASF) often causes high rates of morbidity and mortality, which can reach 100% in domestic swine. To date, serological diagnosis has the drawback of not being able to differentiate variants of this virus. Previous studies have identified the 22 genotypes based on sequence variation in the C-terminal region of the p72 gene, which has become the standard for categorizing ASFVs. This article describes a genotyping assay developed using a segment of PCR-amplified geno… Read more

August 1st, 2013

Via publiDB

Los biofilms y su repercusión en la industria alimentaria

New article published in Tecnoalimen

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