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June, 2014 news

June 27th, 2014

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María  Concepción Porrero Calonge

Detección y caracterización de Staphylococcus aureus procedentes de animales y aguas

PhD Thesis defense by María Concepción Porrero Calonge at the Faculty of Veterinary Medicine of the Complutense University of Madrid

Staphylococcus aureus es un microorganismo comensal del hombre y los animales, que puede dar lugar a procesos patológicos de diferente gravedad, desde infecciones cutáneas a neumonía, endocarditis, septicemia, etc. La aparición de aislados de S. aureus resistentes a meticilina (MRSA) debido a la captación de mecanismos de resistencia a meticilina por parte de aislados sensibles (MSSA) aumenta la gravedad de los procesos clínicos debido a las dificultades en el tratamiento terapéutico. Aunque la detección de MRSA en animales se describió ya en 1972, la detección de MRSA perteneciente a una l… Read more

June 27th, 2014

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XIX ANEMBE International Congress

Risk factors associated to false negative results in bovine tuberculosis diagnosis in-vivo tests in the infected cattle

Oral communication in XIX Congreso Internacional ANEMBE de Medicina Bovina

June 25th, 2014

Servicio de Informática y Comunicación

Technologies Applied to Food Quality and Safety

Technologies Applied to Food Quality and Safety

From 2 to 4 July 2014 will be held in San Lorenzo de El Escorial The Technologies Applied to Food Quality and Safety Course in the Summer Courses of the Complutense University.

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June 25th, 2014

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Veterinary Microbiology

Experimental Lactococcus garvieae infection in zebrafish and first evidence of its ability to invade non-phagocytic cells

Veterinary Microbiology publish this investigation article

Zebrafish has been used for studying infections and host-pathogen interactions in different bacterial fish pathogens. In the present study we evaluated the ability of Lactococcus garvieae to infect zebrafish when inoculated intraperitoneally with 2×107UFC of this pathogen. L. garvieae can colonize and invade zebrafish at multiple anatomical sites causing a lethal acute septicemic infection with clinical signs and lesions consistent with those observed in lactococcosis outbreaks. Immunohistochemical studies showed the presence of L. garvieae into macrophages as well as into non-phagocytic zebra… Read more

June 24th, 2014

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Natalia Sánchez Coppel

Identificación y caracterización molecular de micobacterias no tuberculosas en aislados humanos

Presentation by Natalia Sánchez Coppel to obtain the Master of Research. Faculty of Veterinary Medicine, Complutense University of Madrid

June 23rd, 2014

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Belén Gutiérrez Soriano

Aminoglycoside resistance 16s rRNA methyltransferases: Mechanism of action, biological cost and interference with intrinsic methylations

Belén Gutiérrez Soriano defended the PhD Thesis at the Faculty of Veterinary Medicine of the Complutense University of Madrid

In this work we strive to elucidate this rapidly disseminating resistance mechanism as it is emerging in more and more bacterial pathogens globally. On the one hand we have studied the mechanism of action, biological cost and the relationship between acquired methylations and adjacent intrinsic methylations present in the bacterial ribosome. Furthermore, we have also studied the post-transcriptional methylations of both endogenous and acquired methylases in the aminoglycoside target-site within the rRNAs of Pseudomonas aeruginosa. This microbe was chosen, as aminoglycosides are the first choic… Read more

June 21st, 2014

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The Veterinary Record

Comparison of depopulation and S19-RB51 vaccination strategies for control of bovine brucellosis in high prevalence areas

Investigation published in The Veterinary Record

RB51 vaccination can minimize the diagnostic problems associated with S19 vaccination of adult cattle, but its use for bovine brucellosis (BB) control remains controversial. Here, we describe the evolution of BB prevalence in five high- revalence areas in Spain subjected to different control measures: herd-depopulation of infected herds (I-III) or mass vaccination with RB51 and S19/RB51 vaccination of replacement heifers (IV-V). Annual data from the eradication campaigns was analyzed at a Special Incidence Area (SIA) level and the time to obtain herd-prevalence levels of<1% (“control status”) … Read more

June 11st, 2014

Servicio de Informática y Comunicación



Mass spectrometry better communication at the Tenth Meeting of the SEM Group in Molecular Microbiology 

The VISAVET Centre and Bruker Daltonik GmbH awarded a prize of 300 euros for the mass spectrometry best communication presented in the Tenth Meeting of Molecular Microbiology Group of the Spanish Society for Microbiology. … Read more

June 4th, 2014

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Veterinary Microbiology

Strategic use of serology for the diagnosis of bovine tuberculosis after intradermal skin testing

Investigation published in Veterinary Microbiology

Diagnostic tests based on cell-mediated immunity are used in programs for eradication of bovine tuberculosis (Mycobacterium bovis). Serological assays could be applied as ancillary methods to detect infected animals. Our objective was to evaluate two serological techniques: M. bovis Ab Test (IDEXX, USA) and Enferplex™ TB assay (Enfer, Ireland) in animals tested simultaneously with the single and comparative intradermal tests and the interferon-gamma assay. This work was performed at two stages. First, a preliminary panel of samples collected prior to intradermal tests from tuberculosis-free (n… Read more

June 2nd, 2014

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Workshop on Laboratory Diagnosis of African and Classical Swine Fever

African Swine fever (ASF); what can we do?

Conference in Taller sobre Diagnóstico de laboratorio de África y la peste porcina clásica

June 1st, 2014

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The Veterinary Journal

Characterisation of Streptococcus suis isolates from wild boars (Sus scrofa)

Investigation article published in The Veterinary Journal

Wild boar are widely distributed throughout the Iberian Peninsula and can carry potentially virulent strains of Streptococcus suis. The objective of this study was to determine the prevalence of S. suis in wild boars from two large geographical regions of Spain. Serotypes 1, 2, 7 and 9 identified were further genetically characterised by virulence-associated genotyping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) to determine the population structure of S. suis carried by these animals. Streptococcus suis was isolated from 39.1% of the wild boars examined: ser… Read more

June 1st, 2014

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Research in Veterinary Science

High-throughput multiplex MIRU-VNTR typing of Mycobacterium bovis

Research in Veterinary Science publish this investigation article

Spoligotyping is the most widely used method for genotyping Mycobacterium bovis (M. bovis). However, its discriminatory power varies widely between countries. MIRU-VNTR typing could be a promising alternative, although it generally requires the time consuming and laborious simplex PCR assays using standard agarose gel electrophoresis. The accuracy of this approach depends on good standardization and a certain degree of expertise. This study presents a version of MIRU-VNTR based on three triplex PCRs with automatic allelic assignation of the products analyzed in capillary electrophoresis. The t… Read more

June 1st, 2014

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Journal of Fish Diseases

Lesional patterns associated with mycobacteriosis in an Atlantic horse mackerel, Trachurus trachurus (L.), aquarium population

Investigation published in Journal of Fish Diseases

Mycobacteria are ubiquitous bacteria in the environment, particularly in aquatic reservoirs. They are responsible for a chronic or acute systemic granulomatous disease, named mycobacteriosis, which occurs in wild, aquarium and culture fish (Jacobs et al. 2009). Three Mycobacterium spp. have dominated the literature on fish diseases: Mycobacterium marinum, Mycobacterium fortuitum and Mycobacterium chelonae (Gauthier & Rhodes 2009). Generally, mycobacteriosis in fish displays granulomas produced in multiple organs or tissues, although chronic disease may not produce clinical signs (Gauthier & Rh… Read more

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