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Enterococcus, Salmonella y H2S: Control Remoto de la Resistencia a Antibioticos

Daniel Thomas López defended the PhD Thesis at the Faculty of Veterinary Medicine of the Complutense University of Madrid

Enterococcus faecalis and Enterococcus faecium are among the most significant hospital pathogens globally, producing life threatening endocarditis, urinary tract infections and septicemia. This is due, among other reasons, to their high-level of resilience to adverse environmental conditions, and to their resistance to a wide range of antibiotics. Of all the antimicrobial resistance mechanisms harbored by enterococci, their intrinsic resistance to cephalosporins (an invaluable group of antibiotics within β- lactams family) is the most troubling from a clinical perspective, as it leads to … Read more

Mesa redonda sobre Bioseguridad en explotaciones de vacuno de carne

Conference in Bioseguridad en explotaciones de vacuno de carne

e-ColeVet / VetenVIVO. Formación de profesionales veterinarios. 2017 edition

Communications in this event:

- Inspección post-mortem: principales lecciones en la especie porcina

- Inspección post-mortem: Lesiones especificas en caza mayor y menor

- Limpieza y desinfección de explotaciones ganaderas. Cómo y cuándo hacerlas de manera eficientes

- Patología intestinal en pequeños animales

- Diagnóstico de patologías víricas en caballos

- Leyes de Protección Animal

- Agentes zoonóticos en alimentos y su repercusión en la Salud Pública

- Nuevos reservorios de Leishmaniosis

- Zoonosis transmitidas por garrapatas

- Inspección post-mortem: Principales lesiones en ganado vacuno

- Diagnóstico diferencial enfermedades dermatológicas en pequeños animales

- Utilización de antimicrobianos en ganadería: nuevos retos

Highly Tigecycline-Resistant Klebsiella pneumoniae Sequence Type 11 (ST11) and ST147 Isolates from Companion Animals

Investigation published in Antimicrobial Agents and Chemotherapy

In this study we have characterized two tigecycline resistant Klebsiella pneumoniae clones isolated from dog urine samples. The isolates were genetically unrelated, belonging to sequence types ST11 and ST147, both classically related to human isolates. To the best of our knowledge, this is the first identification of tigecycline resistant isolates from animals. We unveil the worrisome circulation among animals of bacterial clones resistant to this last resort antibiotic… Read more

Related news in other media:

- Descubren en perros bacterias resistentes a antibióticos de último recurso - lainformacion.com

- Descubren en perros bacterias resistentes a antibióticos de último recurso - ecodiario.eleconomista.es

- Investigadores de la Universidad Complutense localizan en perros un patógeno humano resistente a un antibiótico de último recurso - ecoaula.eleconomista.es

- Equipo de la UCM halla bacteria resistente al antibiótico de último recurso - elconfidencial.com

- Equipo de la UCM halla bacteria resistente al antibiótico de último recurso - abc.es

- Equipo de la UCM halla bacteria resistente al antibiótico de último recurso - lavanguardia.com

- Resistanace to antibiotic of last resort found in human pathogens infecting dogs - asm.org

Jeotgalibaca porci sp. nov. and Jeotgalibaca arthritidis sp. nov., two new species isolated from pigs and emended description of the genus Jeotgalibaca

Investigation published in International Journal of Systematic and Evolutionary Microbiology

Biochemical and molecular genetic studies were performed on two novel Gram-positive catalase-negative coccus-shaped organisms isolated from liquid joint samples of two pigs. The microorganisms were not identified as a recognized species based on their cellular, morphological and biochemical tests. 16S rRNA gene sequence comparison studies allowed their identifications as members of the genus Jeotgalibaca, but the organisms were different to Jeotgalibaca dankookensis, the single species of the genus. The two microorganisms shared 96.3% and 96.9% 16S rRNA gene sequence similarity values with the… Read more

Nuevos estudios científicos en materia de diagnóstico y control de tuberculosis. Perspectivas de futuro

Oral communication in Curso de Actualización de Tuberculosis Bovina

Nuevos estudios científicos en materia de diagnóstico y control de tuberculosis. Perspectivas de futuro

Oral communication in Curso de actualización en los aspectos teóricos y de base legal en el diagnóstico de la tuberculosis bovina

6th World Congress on Leishmaniasis

Communications in this event:

- Characterization of Leishmania infantum strains by PCR-RFLP in wild Leporidae from the Community of Madrid

- A new website focused on the control and prevention of leishmaniosis: stopleishmania.org

New tools improve tuberculosis diagnosis in high prevalence settings

Results obtained suggest that serology can be included as ancillary tool for diagnosis of bovine tuberculosis.

A Spanish TB-research consortium including VISAVET, SaBio-IREC, ISCIII, SERIDA and NEIKER recently evaluated the sensitivity of the single cervical intradermal tuberculin test, two interferon-gamma assays and three different antibody detection techniques for bovine tuberculosis diagnosis in a cattle herd with high M. bovis infection prevalence. The results were just published in Research in Veterinary Science. The antibody detection techniques yielded a significant higher sensitivity than that achieved with the official diagnostic techniques. In addition, the interpretation in parallel of cellular and antibody detection techniques reached the highest sensitivity: 98%. Thus, the combined use of diagnostic techniques detecting both cellular and humoral responses could become an alternative in the control of bovine TB outbreaks in high prevalence settings.… Read more

Novos estudos científicos en materia de diagnóstico e control da tuberculose. Perspectivas de futuro

Conference in Xornada Técnica Red Alerta de Galicia Actualización sobre a Tuberculose Bovina

Descripción de laboratorios de Microbiología con nivel III de seguridad en la UCM. Descripción de VISAVET

Oral communication in III Seminario sobre Prevención y defensa frente al uso de agentes biológicos como armas de destrucción masiva

Resistencia a los antibióticos: preguntas frecuentes

3tres3.com publish this article

¿Qué son las resistencias a los antibióticos? ¿Se transmiten entre distintos tipos de bacterias? ¿Una misma bacteria puede ser resistente a varios antibióticos? ¿Los genes de la resistencia son ubicuos? ¿Se pueden medir las resistencias?… Read more

Tuberculin Skin Testing Boosts Interferon Gamma Responses to DIVA Reagents in Mycobacterium bovis-Infected Cattle

Clinical and Vaccine Immunology publish this investigation article

BCG vaccination sensitises cattle to bovine tuberculin which compromises the use of the current bovine TB surveillance tests. Although the performance of a blood test (that utilises antigens expressed by Mycobacterium bovis but not by BCG) capable of discriminating infected from vaccinated animals (DIVA interferon-gamma test, DIT) has been evaluated in naturally infected TB field reactors, there is a need to perform similar analysis in a BCG vaccinated/M. bovis infected population. Furthermore, we explored different scenarios under which a DIT may be implemented alongside BCG vaccination: (i) … Read more

Seminars VISAVET 2017

Communications in this event:

- Implantación del Sistema Líquido Automatizado Bactec TM MGIT TM 960 en el Servicio de Micobacterias y proceso de acreditación

- Uso de herramientas STEM para el estudio de patógenos emergentes: Salmonella en Minnesota

- Bacterias resistentes a colistina y su importancia en Salud Pública y Seguridad Alimentaria

- The control of bovine tuberculosis in India: combining traditional and novel methods

- Introducción a la estadística Bayesiana y su aplicación en la evaluación de técnicas diagnósticas

- Caracterización molecular de aislados de Leishmania infantum en lagomorfos salvajes

- Estudio poblacional de aislados de Staphylococcus epidermidis procedentes de mamitis

- Inmunoproteómica de la bPPD para la identificación de biomarcadores serológicos

- Situación actual y rol del Laboratorio veterinario Oficial en la vigilancia de Tuberculosis y Brucelosis bovina en Uruguay

- Tuberculosis por Mycobacterium bovis en Humanos, Bovinos y Caninos, en una Cuenca Lechera de Hidalgo, México

En busca de una vacuna contra las garrapatas

Vacunas contra garrapatas

Un equipo europeo, en el que participan investigadores españoles, espera conseguir una inmunización contra la picadura de estos artrópodos a finales de 2018…

Characterization of Pasteurella multocida associated with ovine pneumonia using multi-locus sequence typing (MLST) and virulence-associated gene profile analysis and comparison with porcine isolates

Investigation article published in Veterinary Microbiology

Pasteurella multocida is a pathogen causing disease in a wide range of hosts including sheep and pigs. Isolates from ovine pneumonia were characterized by MLST (Multi-host and RIRDC databases) and virulence-associated gene (VAG) typing and compared with porcine isolates. Ovine and porcine isolates did not share any STs as determined by both schemes and exhibited different VAG profiles. With the Multi-host database, sixteen STs were identified among 43 sheep isolates with two STs (ST50 and ST19) comprising 53.5% of the isolates, and seven MLST genotypes (ST3, ST11 and ST62 included 75% of the i… Read more

Evaluation of the Mycobacterium tuberculosis SO2 vaccine using a natural tuberculosis infection model in goats

Investigation article published in The Veterinary Journal

The development of new vaccines against animal tuberculosis (TB) is a priority for improving the control and eradication of this disease, particularly in those species not subjected to compulsory eradication programmes. In this study, the protection conferred by the Mycobacterium tuberculosis SO2 experimental vaccine was evaluated using a natural infection model in goats. Twenty-six goats were distributed in three groups: (1) 10 goats served as a control group; (2) six goats were subcutaneously vaccinated with BCG; and (3) 10 goats were subcutaneously vaccinated with SO2. Four months after vac… Read more

Risk mapping of West Nile virus circulation in Spain, 2015

Acta Tropica publish this investigation article

West Nile fever is an emergent disease in Europe. The objective of this study was to conduct a predictive risk mapping of West Nile Virus (WNV) circulation in Spain based on historical data of WNV circulation. Areas of Spain with evidence of WNV circulation were mapped based on data from notifications to the surveillance systems and a literature review. A logistic regression-based spatial model was used to assess the probability of WNV circulation. Data were analyzed at municipality level. Mean temperatures of the period from June to October, presence of wetlands and presence of Special Protec… Read more

Prevalence of Escherichia coli virulence genes in patients with diarrhea and a subpopulation of healthy volunteers in Madrid, Spain

The book Applications of STEM (Science, Technology, Engineering and Mathematics) tools in microbiology of infectious diseases edited by Frontiers includes this chapter

Etiological diagnosis of diarrheal diseases may be complicated by their multi-factorial nature. In addition, Escherichia coli strains present in the gut can occasionally harbor VGs without causing disease, which complicates the assessment of their clinical significance in particular. The aim of this study was to detect and quantify nine VGs (stx1, stx2, eae, aggR, ehxA, invA, est and elt) typically present in five E. coli enteric pathotypes (EHEC, ETEC, EPEC, EAEC and EIEC) in fecal samples collected from 49 patients with acute diarrhea and 32 healthy controls from Madrid, Spain. In addition,… Read more

Assessment of MALDI-TOF MS as alternative tool for Streptococcus suis identification

Chapter included in Applications of STEM (Science, Technology, Engineering and Mathematics) tools in microbiology of infectious diseases edited by Frontiers

The accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identifying Streptococcus suis isolates obtained from pigs, wild animals, and humans was evaluated using a PCR-based identification assay as the gold standard. In addition, MALDI-TOF MS was compared with the commercial multi-tests Rapid ID 32 STREP system. From the 129 S. suis isolates included in the study and identified by the molecular method, only 31 isolates (24.03%) had score values ≥2.300 and 79 isolates (61.24%) gave score values between 2.299 and 2.000. After updating … Read more

Vigilancia Epidemiológica de la Leishmaniosis mediante la Técnica de Inmunofluorescencia Indirecta (IFI)

The book Brote de Leishmaniasis en Fuenlabrada y Otros Municipios de la Comunidad de Madrid. El papel de las liebres y los conejos como reservorios edited by Comunidad de Madrid includes this chapter

La epidemiología de la leishmaniasis es muy compleja. En la leishmaniasis producida por L.infantum son muchos los animales, tanto salvajes como domésticos, que contribuyen junto al perro, considerado el principal reservorio, al mantenimiento del parásito. Prueba de ello es que durante el último brote que ha tenido lugar en la Comunidad de Madrid (2009-2015) se han descrito por vez primera como reservorios del parásito a los lagomorfos (liebres y conejos). Es pues de gran importancia un buen programa de vigilancia epidemiológica para atajar este problema de salud pública. Ello pasaría por detec… Read more