Home \ Research \ Scientific publications \


Use of processing fluids and serum samples to characterize porcine reproductive and respiratory syndrome virus dynamics in 3 day-old pigs

Investigation published in Veterinary Microbiology

November 1st, 2018

Collection of serum samples of pigs at weaning to monitor for porcine reproductive and respiratory syndrome virus (PRRSV) has become a common practice to determine PRRSV herd infection status. Diagnostic sensitivity of this practice is low in herds undergoing PRRSV elimination once prevalence of infection is near zero. Thus, the goal of this study was to characterize the dynamics of PRRSV infection in 3 day-old pigs overtime using serum and serosanguineous fluids obtained as part of castration and tail docking practices (processing fluids (PF)). Secondary goal was to estimate sensitivity and specificity of PF in the 3 day old population. A 6000 breed-to-wean sow herd was monitored every three weeks for 23 weeks after a PRRSV outbreak by collecting both PF and individual serum samples from all pigs in the selected litters. Out of the 77 litters tested, 23 (29.8%) were identified as positive using the PF and the serum samples, with a Cohen’s kappa statistic of 0.81 (95% CI: 0.59–1) between the results obtained in each sample type. The sensitivity and specificity of the PF relative to the results in serum was 87% (95% CI: 66%–97%) and 94% (95% CI: 85%–99%) respectively. The percentage of PRRSV positive litters decreased over time and litters from gilts were more likely to test positive than those from older sows. Overall, the study demonstrates that PF can be a convenient and reliable specimen to monitor PRRSV infection in breeding herds




Vilalta C., Sanhueza J., Alvarez J., Murray D., Torremorell M., Corzo C. and Morrison R.




See this article
Use of processing fluids and serum samples to characterize porcine reproductive and respiratory syndrome virus dynamics in 3 day-old pigs

See it on NLM PubMed
Use of processing fluids and serum samples to characterize porcine reproductive and respiratory syndrome virus dynamics in 3 day-old pigs



Participants:

University of MinnesotaDepartment of Veterinary Population Medicine. College of Veterinary Medicine. University of Minnesota (UMM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

New Fashion PorkNew Fashion Pork.

Universidad ComplutenseCentro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Veterinary Microbiology
FACTOR YEAR Q
2.791 2018

NLMID: 7705469

PMID: 30293648

ISSN: 0378-1135



TITLE: Use of processing fluids and serum samples to characterize porcine reproductive and respiratory syndrome virus dynamics in 3 day-old pigs


JOURNAL: Vet. Microbiol.


NUMERACIÓN: 225:149-156


AÑO: 2018


PUBLISHER: Elsevier Science Publishers


AUTHORS: Vilalta C., Sanhueza J., Alvarez J., Murray D., Torremorell M., Corzo C. and Morrison R.


VISAVET PARTICIPANTS


Julio Álvarez Sánchez

DOI: https://doi.org/ 10.1016/j.vetmic.2018.09.006


CITE THIS PUBLICATION:

Vilalta C., Sanhueza J., Alvarez J., Murray D., Torremorell M., Corzo C. and Morrison R. Use of processing fluids and serum samples to characterize porcine reproductive and respiratory syndrome virus dynamics in 3 day-old pigs. Veterinary Microbiology. 225:149-156. 2018. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2018.09.006