Integron Identification in Bacterial Genomes and Cassette Recombination Assays
El libro Horizontal Gene Transfer editado por Humana Press Inc. recoge este capítulo
5 de octubre de 2019
Vit C., Loot C., Escudero JA., Nivina A. y Mazel D.
Integrons are genetic elements involved in bacterial adaptation to the environment. Sedentary chromosomal integrons (SCIs) can stockpile and rearrange a myriad of different functions encoded in gene cassettes. Through their association with transposable elements and conjugative plasmids, some SCIs have acquired mobility and are now termed Mobile Integrons (MIs). MIs have reached the hospitals and are involved in the rise and spread of antibiotic resistance genes through horizontal gene transfer among numerous bacterial species. Here we aimed at describing methods for the detection of integrons in sequenced bacterial genomes as well as for the experimental characterization of the activity of their different components: The integrase and the recombination sites
 | Bacterial Genome Plasticity (BGP). Department of Genomes and Genetics. Institut Pasteur. |
 | UMR3525. Centre National de la Recherche Scientifique (CNRS). |
 | Collège doctoral. Université Sorbonne Paris Descartes. |
| Université Sorbonne Paris Descartes. |
