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MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates From Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage

Pathogens publica este artículo de investigación

22 de junio de 2020

Brucellosis is a common zoonotic disease in Egypt. However, there are limited data available on the genetic diversity of brucellae circulating in Egypt and other Mediterranean areas. One hundred and nine Brucella (B.) strains were isolated from different animal species in thirteen Egyptian governorates. Multi-locus variable number tandem repeats (VNTRs) analysis (MLVA-16) was employed to determine the geographical relatedness and the genetic diversity of a panel of selected Egyptian strains (n = 69), with strains originating from Italy (n = 49), Portugal (n = 52), Greece (n = 63), and Tunisia (n = 4). Egyptian B. melitensis strains clustered into two main clusters containing 21 genotypes. Egyptian B. abortus strains clustered into three main clusters containing nine genotypes. The genotypes were irregularly distributed over time and space in the study area. Egyptian strains of B. melitensis showed MLVA-16 patterns closer to that of Italian strains. Egyptian B. abortus strains isolated from cattle share the same genotype with strains from Portugal and similar to strains from Italy with low genetic diversity. Strains with similar MLVA patterns isolated from different governorates highlight the movement of the pathogen among governorates. Hence, it may also reflect the long endemicity of brucellosis in Egypt with earlier dispersal of types and great local genetic diversity. Open markets may contribute to cross-species transmission and dissemination of the new types nationwide. The presence of West Mediterranean lineages of B. melitensis and relatedness of B. abortus strains from the studied countries is a result of the socio-historical connections among the Mediterranean countries. Transnational eradication of brucellosis in the Mediterranean basin is highly demanded




Wareth G., El-Diasty M., Melzer F., Schmoock G., Moustafa SA., El-Beskawy M., Khater DF., Hamdy MER., Zaki HM., Ferreira AC., Ekateriniadou LV., Boukouvala E., Abdel-Glil MY., Menshawy AMS., Perez-Sancho M., Sakhria S., Pletz MW. y Neubauer H.




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MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates From Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage

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MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates From Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage


Participantes:

Friedrich Loeffler Institut-Bundesforschungsinstitut fuer TiergesundheitInstitute of Bacterial Infections and Zoonoses. Friedrich Loeffler Institut-Bundesforschungsinstitut fuer Tiergesundheit (FLI).

Jena University HospitalInstitute for Infectious Diseases and Infection Control. Jena University Hospital.

Benha UniversityFaculty of Veterinary Medicine. Benha University (BU).

Animal Health Research InstituteDepartment of Brucellosis. Animal Health Research Institute (AHRI).

Matrouh UniversityDepartment of Animal Medicine. Faculty of Veterinary Medicine. Matrouh University.

National Institute of Agrarian and Veterinary ResearchNational Reference Laboratory for Brucellosis. National Institute of Agrarian and Veterinary Research.

Universidade de LisboaFaculdade de Ciências. Universidade de Lisboa (ULisboa).

Universidade de LisboaBiosystems and Integrative Sciences Institute (BioISI). Universidade de Lisboa (ULisboa).

Hellenic Agricultural Organisation DEMETERVeterinary Research Institute. Hellenic Agricultural Organisation DEMETER.

Beni-Suef UniversityFaculty of Veterinary Medicine. Beni-Suef University.

Universidad ComplutenseCentro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

University of Tunis El ManarInstitute of Veterinary Research of Tunisia. University of Tunis El Manar.







Pathogens
FACTOR YEAR Q
3.018 2019

NLMID: 101596317

PMID: 32580472

ISSN: 2076-0817



TÍTULO: MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates From Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage


REVISTA: Pathogens


NUMERACIÓN: 9(6):E498


AÑO: 2020


EDITORIAL: MDPI


AUTORES: Wareth G., El-Diasty M., Melzer F., Schmoock G., Moustafa SA., El-Beskawy M., Khater DF., Hamdy MER., Zaki HM., Ferreira AC., Ekateriniadou LV., Boukouvala E., Abdel-Glil MY., Menshawy AMS., Perez-Sancho M., Sakhria S., Pletz MW. and Neubauer H.


PARTICIPANTES VISAVET


Marta Pérez Sancho

DOI: https://doi.org/10.3390/pathogens9060498


CITA ESTA PUBLICACIÓN:

Wareth G., El-Diasty M., Melzer F., Schmoock G., Moustafa SA., El-Beskawy M., Khater DF., Hamdy MER., Zaki HM., Ferreira AC., Ekateriniadou LV., Boukouvala E., Abdel-Glil MY., Menshawy AMS., Perez-Sancho M., Sakhria S., Pletz MW. y Neubauer H. MLVA-16 Genotyping of Brucella abortus and Brucella melitensis Isolates From Different Animal Species in Egypt: Geographical Relatedness and the Mediterranean Lineage. Pathogens. 9(6):E498. 2020. (A). ISSN: 2076-0817. DOI: 10.3390/pathogens9060498