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16S-23S rRNA Internal Transcribed Spacer Region ( ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri

Microorganisms publish this investigation article

May 17th, 2023

Lactococcus garvieae is the etiological agent of lactococcosis, a clinically and economically significant infectious disease affecting farmed rainbow trout. L. garvieae had been considered the only cause of lactococcosis for a long time; however, L. petauri, another species of the genus Lactococcus, has lately been linked to the same disease. The genomes and biochemical profiles of L. petauri and L. garvieae have a high degree of similarity. Traditional diagnostic tests currently available cannot distinguish between these two species. The aim of this study was to use the transcribed spacer (ITS) region between 16S rRNA and 23S rRNA as a potential useful molecular target to differentiate L. garvieae from L. petauri, saving time and money compared to genomics methods currently used as diagnostic tools for accurate discrimination between these two species. The ITS region of 82 strains was amplified and sequenced. The amplified fragments varied in size from 500 to 550 bp. Based on the sequence, seven SNPs were identified that separate L. garvieae from L. petauri. The 16S-23S rRNA ITS region has enough resolution to distinguish between closely related L. garvieae and L. petauri and it can be used as a diagnostic marker to quickly identify the pathogens in a lactococcosis outbreak




Stoppani N., Colussi S., Pastorino P., Prearo M., Sciuto S., Altinok I., Ozturk RC., Ture M., Vela AI., Blanco MM., Kotzamanidis C., Bitchava K., Malousi A., Fariano L., Volpatti D., Acutis PL. and Fernandez-Garayzabal JF..




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16S-23S rRNA Internal Transcribed Spacer Region ( ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri

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16S-23S rRNA Internal Transcribed Spacer Region ( ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri



Participants:

Istituto Zooprofilattico Sperimentale del Piemonte (IZSTO).

Surmene Faculty of Marine Sciences. Karadeniz Technical University (KTU).

Central Fisheries Research Institute (SUMAE).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Veterinary Research Institute. Elgo-Dimitra Thessaloniki.

Agricultural University of AthensSchool of Animal Biosciences. Agricultural University of Athens (AUA).

Laboratory of Biological Chemistry. Medical School. Aristotle University of Thessaloniki.

Azienda Agricola Canali Cavour S.S (AACC).

Department of Agricultural, Food, Environmental and Animal Sciences. Università degli Studi di Udine (UNIUD).







Microorganisms
FACTOR YEAR Q
4.500 2022

PMID: 37317294

ISSN: 2076-2607



TITLE: 16S-23S rRNA Internal Transcribed Spacer Region ( ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri


JOURNAL: Microorganisms


NUMERACIÓN: 11(5):1320


AÑO: 2023


PUBLISHER: MDPI AG


AUTHORS: Stoppani N., Colussi S., Pastorino P., Prearo M., Sciuto S., Altinok I., Ozturk RC., Ture M., Vela AI., Blanco MM., Kotzamanidis C., Bitchava K., Malousi A., Fariano L., Volpatti D., Acutis PL. and Fernandez-Garayzabal JF..


9th
Ana Isabel Vela Alonso
Last
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.3390/microorganisms11051320


CITE THIS PUBLICATION:

Stoppani N., Colussi S., Pastorino P., Prearo M., Sciuto S., Altinok I., Ozturk RC., Ture M., Vela AI., Blanco MM., Kotzamanidis C., Bitchava K., Malousi A., Fariano L., Volpatti D., Acutis PL. and Fernandez-Garayzabal JF. 16S-23S rRNA Internal Transcribed Spacer Region ( ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri. Microorganisms. 11(5):1320. 2023. (A). ISSN: 2076-2607. DOI: 10.3390/microorganisms11051320


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